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[Objective] To develop a process for separating and purifying total flavonoids from licorice waste residue using macroporous resin. [Method] Comparing the adsorption capacity and desorption rate of D101B, AB-8 and DM-130 for total flavonoids to screen the optimal resin. Single factor experiments were used to screen the sample concentration, sample flow rate, sample volume, 20% ethanol elution volume, eluent concentration and eluent volume during the dynamic adsorption process. [Result] AB-8 macroporous resin had the best separation and purification effect for total flavonoids in licorice waste residue. The sample concentration was 2.345-3.126 mg/mL, the sample flow rate was 1.01.5 mL/min, and the sample volume was 64 mL, 20% ethanol elution volume was 5 BV, and 80% ethanol elution volume was 4 BV. After this purification process, the total flavonoid concentration increased from 15.63% to 65.68%. [Conclusion] This method is suitable for the preliminary separation and purification of total flavonoids from licorice waste residue. [ABSTRACT FROM AUTHOR]