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Copyright of Journal of Ordnance Equipment Engineering is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The MoGAD gene in Moringa oleifera was identified, and subsequent cloning, bioinformatics analysis, and expression level analysis were conducted. These investigations have established a solid foundation for exploring the functional relationship between the MOGAD genes and γ-aminobutyric acid (GABA) biosynthesis in M. oleifera. (Method) Using the GAD protein sequences of Arabidopsis thaliana (NP_197235.1) and Vitis vinifera (XP_002285268.1) to be as references, MOGAD family genes were identified from M. oleifera tran-scriptome data through local BLAST method and TBtools software. The genes were subsequently cloned using PCR technology. Bioinformatics online analysis websites and molecular docking technology were employed to analyze the physical and chemical properties, as well as the catalytic active sites of the MoGAD protein. Additionally, the real-time fluorescence quantitative RT-qPCR technology was utilized to assess the expression levels of MoGAD genes in different tissues. [Result] The four MoGAD genes were excavated from transcriptome data and cloned successfully. The length of the CDS region sequenced was 1494, 1212, 1470 and 942 bp respectively. MOGADI, MoGAD3 and MoGAD4 proteins had no transmembrane domains and belonged to extracellular proteins. The MoGAD2 gene had the transmembrane structure at amino acid positions 5-27 and 60-82, which encoded a kind of secreted proteins. The phylogenetic analysis showed that the largest number of motifs was in the branch of MoGADI gene family. The molecular docking revealed that four MoGAD proteins had binding sites for pyridoxal phosphate (PLP) and L-glutamate, and each protein had multiple amino acid residues that formed hydrogen bonds with PLP and L-glutamate. The results of fluorescence quantitative analysis showed that the expression levels of MoGADI and MoGAD2 genes in tender leaves, mature leaves and flowers were significantly higher than those of MoGAD3 and MoGAD4 genes, but the expression levels of these genes were relatively low in the stem, suggesting that MoGAD enzymes were mainly involved in GABA biosynthesis in the leaves of M. oleifera. Conclusion] MoGAD gene is successfully cloned from M. oleifera, and the physical and chemical properties of its encoded protein and the functional active sites of substrate binding are analyzed, which provide a theoretical basis for further study on the function of MoGAD genes. [ABSTRACT FROM AUTHOR]

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Ballistics / Dandao Xuebao is the property of Journal of Ballistics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Na-Se battery is consider ed to have high research value because of its high theoretical capacity. However, there is still problems of weak large rate performance and low capacity of charge and discharge. It is necessary to develop materials that could resolve the above problems. In this research, nickel was used to catalyze the in-situ growth of carbon nanotubes by one-step pyrolysis, and the product (Ni/CTs) was used to modify the polypropylene membrane to improve the electrochemical performance of Na-Se battery. As a result, membranes modified with Ni/CT s showed good cycling and rate performance in Na-Se battery, it still h as 354 m A h g-1 specific capacity aft er 100 cycles at 0. 2 C and 213 m A h g-1 specific capacity at 20 C. And it still has a specific capacity of 283.7mAh g-1 at 1 C. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Mass Spectrometry Society is the property of Journal of Chinese Mass Spectrometry Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tea Science is the property of Journal of Tea Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oncology is the property of Chinese Journal of Oncology / Zhonghua Zhongliu Zazhi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Taken 10 low molecular compounds( thymol, cinnamyl aldehyde, salicylic acid( SA), sulfosalicylic acid, ethyl p-hydroxybenzoate, 3-amino-butyricacid( BABA), benzothiadiazole( BTH), 2, 5-dihydroxybenzoic acid, methyl jasmonate, harpin) as activators, characteristics of the induced changes of protect enzymes as well as the main bio-chemical components in tea plant were checked.The results showed that all activators could induce the increase of soluble protein content in tea plant except for 3-amino-butyricacid, and all other activators could also induce the increase of amino acid in tea leaves. On the other side though salicylic acid, sulfosalicylic acid, ethyl p-hydroxybenzoate, 2, 5-dihydroxybenzoic acid were similar in molecular structure but induced a much different change of the main bio-chemicals in tea plant. Compared with salicylic acid, the variation amplitude of bio-chemicals induced by sulfosalicylic acid, ethyl p-hydroxybenzoate, 2, 5-dihydroxybenzoic acid was greater, and 2, 5-dihydroxybenzoic acid also induced the increase of the enzyme activity of POD and PPO obviously. Harpin inducing activity of PAL decreased sharply, while BTH inducing the PAL activity increased distinctly, but the polyphenol contents changed a little under the treatments of both activators, which indicated that there still were a lot of things to be understand between the relationship of PAL and tea polyphenols. With thymol, cinnamyl aldehyde inducing, the POD, PPO activities of tea plant decreased but the coffine content in tea leaves increased, which indicated that these two materials might have some special physiology function on tea plant. [ABSTRACT FROM AUTHOR]