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目的探究磷酸二酯酶5（PDE5）抑制剂西地那非（SIL）或LW1646对单侧输尿管结扎（UUO）诱导的肾间质纤维化的保护作用。方法C57BL/6雄性小鼠被随机分为假手术Sham（n=6）、7UUO（n=6）、7UUO+SIL（n=6）或7UUO+LW1646（n=6）组，术前1h给予SIL或LW1646、或溶剂灌胃给药，连续给药7 d后处死小鼠，收集梗阻侧肾脏标本。对肾组织进行H&E和Masson’s染色，使用免疫印迹和RT-qPCR检测肾脏中的纤维化、内质网应激、自噬、凋亡相关蛋白表达水平以及纤维化相关基因表达水平。使用人近端小管上皮细胞（HK-2）给予TGF-β1 48 h，检测cGMP对内质网应激和纤维化相关蛋白水平的影响。使用衣霉素孵育HK-2细胞24 h，检测PDE5抑制剂对内质网应激、纤维化相关蛋白、自噬和凋亡蛋白水平的影响。结果在行UUO术后7 d小鼠体质量相对于假手术组显著下降（P P

目的探讨激活胆汁酸受体TGR5在单肾缺血再灌注损伤合并对侧肾摘除（uIRIx）模型诱导的肾脏纤维化中的作用。方法体内实验：将C57BL/6J小鼠随机分成假手术（Sham）组、uIRIx组及uIRIx+石胆酸（LCA）组，每组6只，使用uIRIx模型诱导肾脏纤维化，通过血和尿生化指标评估肾脏功能，利用HE染色评估肾脏损伤程度，使用Masson染色及免疫组化对肾脏纤维化程度进行评估，并用Western Blotting检测肾脏皮质纤维化相关指标蛋白表达；分别在TGR5+/+小鼠及TGR5-/-小鼠中设置Sham组及uIRIx组，每组6只，利用Western Blotting检测各组肾脏纤维化程度。体外实验：在人源肾上皮细胞系HK2细胞中给予TGF-β1诱导促纤维化反应，给予LCA进行药物干预，利用鬼笔环肽染色标记细胞骨架，使用Western Blotting检测HK2细胞内纤维化相关指标蛋白表达。结果体内实验：与Sham组相比，uIRIx组小鼠血浆肌酐水平（P=0.007）及尿白蛋白/肌酐比（P=0.041）明显增加，肾脏皮质蛋白TGR5表达（P=0.002）下降，Fibronectin表达（P=0.020）及COL1A1表达（P<0.001）上升，同时伴有肾脏结构受损及胶原沉积加重，LCA干预有效改善肾脏功能，缓解肾脏损伤及纤维化程度；TGR5+/+小鼠与TGR5-/-小鼠相比，uIRIx诱导引起的Fibronectin表达（P<0.001）及COL1A1表达（P=0.001）增加。体外实验：TGF-β1诱导HK2细胞形态改变，细胞骨架解聚重组，促纤维化指标蛋白上调；LCA可有效抑制TGF-β1诱导的细胞形态改变及骨架解聚重组，呈浓度依赖性下调纤维化相关指标蛋白表达。结论LCA缓解uIRIx模型诱导的肾脏纤维化，TGR5基因敲除加重uIRIx诱导的肾脏纤维化；在HK2细胞中，LCA缓解TGF-β1诱导的细胞促纤维化反应。研究结果提示激活TGR5缓解缺血再灌注后肾脏纤维化损伤进程。

Objective To investigate the effect of crisis management in patient complaint management in the pediatric dentistry clinic. Methods Totally 200 cases of pediatric patients who visited the pediatric dentistry clinic between January and December 2021 were included as the control group, and routine nursing management was carried out during the treatment. Another 200 cases of pediatric patients who visited the pediatric dentistry clinic between January and December 2022 were recruited as the study group, and a crisis management model was adopted. The complaint rate, praise rate and satisfaction with nursing service were measured and compared between two groups. Results The complaint rate in the study group was lower than that in the control group (1. 00% vs. 5. 50%, P＜0. 05). The praise rate in the study group was higher than that in the control group (12. 00% vs. 4. 50%, P＜0. 01). The rate of satisfaction with working attitude, humanistic care, clinic procedure, health education and overall satisfaction rate in the study group were higher than those in the control group(P＜0. 05). Conclusion The crisis management is effective to reduce the rate of patient complaint and improve the nursing quality in the pediatric dentistry clinic. It is an effective strategy to facilitate the healthy and stable development of pediatric dentistry clinic (目的 探讨危机管理理论在儿童口腔科门诊患者投诉管理中的应用效果。方法 选取2021年1月—12月就诊于儿童口腔科的200例患儿为对照组, 实施常规护理管理; 2022年1月—12月就诊的200例患儿为研究组, 实施危机管理。对比实施危机管理前后护理投诉发生率、表扬率以及患儿家属满意度。结果 实施护理危机管理后, 研究组护理投诉率1. 00%(2/200), 较对照组5. 50%(11/200)降低, 差异有统计学意义(P＜0. 05)。研究组受到患儿家属表扬24(12. 00%)人次, 较对照组9(4. 50%)人次上升, 差异有统计学意义(P＜0. 01)。工作态度、人文关怀、就诊流程、健康教育及总满意评价研究组均高于对照组, 差异有统计学意义(P＜0. 05)。结论 应用危机管理理论可以有效降低儿童口腔科门诊患者投诉事件的发生率, 提升儿童口腔专科护理质量, 有利于促进科室护理工作的健康、稳定发展。)

With the advancement of vitrification freezing technology, frozen-thawed embryo transfer (FET) plays a crucial role in assisted reproductive technology by preventing ovarian hyperstimulation syndrome, reducing ectopic pregnancy rates, and enhancing cumulative pregnancy rates. The proportion of FET cycles in assisted reproduction has been increasing annually, establishing FET as a key component of reproductive technologies. The outcome of FET pregnancies is influenced by various factors, including age, embryo quality, endometrial thickness, endometrial preparation protocols, DNA fragmentation, and Y chromosome microdeletions. Age, embryo quality, and endometrial receptivity are pivotal factors determining the success of FET treatments, directly impacting embryo development, implantation, and pregnancy success rates. Therefore, identifying the influencing factors of FET pregnancy outcome, so as to implement more precise, personalized and targeted prevention to improve pregnancy rates, has become a research hotspot. This review aims to summarize the relevant factors affecting the outcome of FET pregnancies, providing a reference for clinical decision. [ABSTRACT FROM AUTHOR]

In the background of the new agricultural science, universities need to focus on the fundamental task of cultivating morality and talents, focusing on national major strategies such as rural revitalization, and cultivating a group of applied innovative talents with solid professional knowledge, practical and innovative abilities, as well as understanding agriculture, loving rural areas, and loving farmers. The cultivation of talents relies on the construction of high-quality professional courses. Horticultural plant breeding is a major backbone course of the horticulture major. Through preliminary exploration and practice, this course has constructed a "Trinity" ideological and political teaching system, which has certain reference significance for the ideological and political construction of agricultural related courses. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

An environmental strategy has been initiated, called “ecological protection red lines”, in China. The population, resources and environment can be balanced to promote the economic, social and ecological benefits. The ecological restoration zoning of national land space can be implemented to improve the habitat quality, the regional ecological security and the sustainable development. It is very necessary to identify the ecological source areas with the ecological protection red line as the initial scope, in order to build a scientific and reliable ecological security network. The “production -living-ecological” space can be classified into the land-space ecological restoration zoning. Therefore, taking Sanming City of Fujian Province as the research area, this study aims to construct a more scientific and effective technical paradigm of land-space ecological restoration zoning. Firstly, the importance of ecological protection was assessed to verify the boundaries of prohibited development zones and protected areas, according to the importance of ecosystem services and ecological vulnerability. Then the ecological source areas were identified to delimit the ecological protection red line. Secondly, the resistance factors were considered to construct the evaluation system of expanding resistance for the ecological source land. And the minimum cumulative resistance model was used to evaluate the ecological corridors and nodes. The ecological fracture points were then extracted to construct a scientific and reliable pattern of ecological security. Finally, the land-space ecological restoration zoning was carried out, according to the ecological security level and the scope of “production-living-ecological” space. Then the targeted countermeasures were proposed for the ecological restoration and protection. The research results showed that: 1) There were 34 ecological sources with an area of 2 185.44 km², accounting for about 9.5% of the national territorial area of the City. These ecological sources were evenly distributed in the surrounding hills and mountains around the central basin, which were mainly concentrated in the North of City. 2) 12 important ecological corridors and 70 general ecological corridors were identified to extract 30 important ecological nodes and 47 general ecological nodes, together with 66 ecological breaking points. There were the full pattern and high level of ecological security in general. However, the corridors in the southern region were sparse with the dense ecological breaking points and low network connectivity. 3) The grade of ecological security was divided to superimpose the scope of “production-living-ecological” space. 12 types of ecological restoration areas were divided into four categories, including the core key zone (16.13%), monitoring and early warning zone (25.04%), buffer transitional zone (35.53%), and conservation protective zone (23.30%). The ecological protection was proposed for the different restoration zones. As such, the regional ecological security was achieved in the high-quality development of cities in the southern hilly areas. Ecological restoration planning can be expected to effectively construct in the technical paradigm of ecological restoration zoning of land space, The finding can provide the technical reference for the decision-making on the land space and ecological protection in the hilly areas of South China. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[ Objective/Meaning] Based on the theory of industrial correlation, the analysis on the correlation mechanism of agriculture between Fujian and Taiwan could provide theoretical and practical reference for the development of agricultural integration between Fujian and Taiwan. [Methods/Procedures]By taking the Phalaenopsis aphrodite industry in Zhangpu County of Fujian Province as an example, the analysis framework of “ production linkage-institutional linkage-market linkage” was constructed to analyze the correlation mechanism and practical dilemma of agriculture between Fujian and Taiwan through the methods of field investigation and in-depth interview. And then the strategies for promoting the integration of agriculture between Fujian and Taiwan were explored. [ Results/Conclusions] The study found that: (1) The Phalaenopsis aphrodite industry in Zhangpu could provide a typical case for the expansion and extension of industrial relevancy theory. The industrial relevancy theory could provide theoretical support for the further research on the strategy of agricultural integration between Fujian and Taiwan. (2) The agriculture in Fujian and Taiwan has established the linkages of production, institution and market at the regional and enterprise levels, and the knowledge spillover effect based on the technological diffusion has promoted the development of local agriculture. However, there were some practical difficulties in the integration of agriculture between Fujian and Taiwan, such as the lack of key technologies for breeding, the insignificant policy effect and the lock-in of product market evolution path. (3) Comprehensively supporting to carry out the breeding work, exerting the remarkable external policy effect and accelerating the breakthrough of evolution path for product market were the effective measures to get out of the current dilemma of agricultural integrated development between Fujian and Taiwan. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】 The study aims to explore the effects of reducing nitrogen fertilizer combined with microbial agent application on flue-cured tobacco yield and soil microbial diversity. 【Method】 Using Yunyan 87 as the experimental material, four treatments were set up: T1 local conventional fertilization (CK), T2 conventional fertilization + microbial agent, T3 conventional fertilization + 10% reduction of nitrogen fertilizer+ microbial agent, and T4 conventional fertilization + 20% reduction of nitrogen fertilizer + microbial agent. The agronomic traits of flue-cured tobacco at different stages, the economic traits of flue-cured tobacco leaves, and the diversity of rhizosphere soil microbial communities at maturity were measured for each treatment. 【Result】 There was no significant difference in the agronomic traits of flue-cured tobacco among different treatments and periods. This indicates that nitrogen reduction treatment did not affect the growth of flue-cured tobacco. The economic traits of flue-cured tobacco among different treatments showed no significant difference in the output value of T2 and T3 treatments compared to T1 treatment. The output value of T4 treatment was 1 351.56 yuan/667 m², which was a significant difference compared to T2 treatment. Compared with T1 treatment, there was no significant difference in soil physicochemical properties or indicators among different treatments. (2) Each treatment with microbial agents increased the total and unique OTUs of bacteria and fungi. Compared to T1 treatment, the T2 treatment increased the unique OTUs of bacteria by 68.4%, the total OTUs of fungi treated with T2 and T3 increased by 60.2% and 54.9%, respectively, T2 and T3 increased the unique OTUs of fungi by 85.2% and 78.2%, respectively, as well as the fungal richness (Chao index) of T2 and T3 treatments. (3) At the phylum level, the relative abundance of Proteobacteria in T4 treatment was significantly higher than that in other treatments, and there was no significant difference in the level of fungi under different treatments. (4) The relative abundance of s_ Fusarium_equiseti in T1 treatment was higher than that in other treatments. T4 treatment increased the number of beneficial microorganisms, such as c_Alphaproteobacteria, o_Sphingosine, g_Gemmatimonas, and c_Bacteroidota, significantly. 【Conclusion】 The combination of microbial agents and a moderate reduction of nitrogen fertilizer application (10%) increased the output value of flue-cured tobacco by 12.5% compared to conventional fertilization, while also improving the composition of soil microbial communities. [ABSTRACT FROM AUTHOR]

Cropland is one of the most important resources in a complex ecosystem with the highest degree of human dependence, in order to ensure the regional food and ecological security for the better settlement environment. The coordinated development can be expected to promote in the multi-functions of cropland for the scientific protection and rational utilization of cropland. Therefore, it is very necessary to scientifically evaluate the spatiotemporal changes of multi-functions of cropland, and to clarify the change of trade-off and synergy among various functions of cropland, due to the natural supply and the demand of human society. Taking Fujian Province in southeastern China as an example, this study aims to construct a performance metrics, according to four dimensions of cropland production, ecological, social, and landscape functions. The multi-functional level of cropland was evaluated on the county scale. The comprehensive analysis was introduced to evaluate the multi-functions of cropland, and then to explore the spatial-temporal variation from 2005 to 2020. Pearson product-moment correlation coefficient was selected to analyze the trade-off and synergy relationships among the functions of each dimension. The results showed that: 1) The multi-functional level of cropland decreased slightly in the most areas. Specifically, the high-value areas were concentrated in the eastern and western regions, with the superior productive and ecological function of cropland. The higher ability was found to guarantee the living and employment of farmers. Among them, the production function showed an upward trend, while the ecological function decreased slightly. Both social and landscape functions were improved significantly. The spatial change of social function was weakened from the mountain to the coastal area, whereas, there was the strong spatial change of landscape function. 2) Synergy relationships were dominated among cropland multi-functions. Only the production-ecological and production-landscape function showed the synergy relationship in more than 50% of the areas. The proportion of grid quantity with the trade-off relationship was ranked in the descending order of the ecological-social, production-social, social-landscape, and ecological-landscape function. A spatial heterogeneity was observed in the trade-off and synergy relationship of each function combination. 3) The present condition of cropland was used to determine the characteristics of agricultural production in the different regions. The single function of cultivated land was combined with the trade-off between different functions. The cropland functional areas were divided into four types, including the superiority protection area (38.52%), strict control area (36.71%), remediation area (6.98%), and transition or exit area (17.79%). Some differentiated management and control suggestions were proposed as well. The superiority protection areas were distributed mainly in the eastern and western regions, while the strict control areas were distributed mainly in the coastal areas of northern and southern regions, and the remediation areas were in the Zhangzhou region of Southern regions, as well as the transition or exit areas were scattered throughout the cities. The findings can provide an important decision-making support to protect the cropland resources. The coordinated development can be expected to promote the multi-functions of cropland for the high efficiency of cropland land use. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guangdong Agricultural Sciences is the property of South China Agricultural University, Guangdong Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Agricultural Outlook (1673-3908) is the property of Institute of Agricultural Information, Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Mountain Research (10082786) is the property of Mountain Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bulletin of Soil & Water Conservation is the property of Bulletin of Soil & Water Conservation Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effect of His-bundle pacing (HBP) on left ventricular structure and function in patients. Methods 47 patients who underwent permanent pacemaker implantation due to high grade atrioventricular block or sick sinus syndrome in the department of cardiology of the hospital from September 2018 to September 2019 were selected and divided into HBP group (23 cases) and right ventricular pacing group (24 cases) . The left ventricular end diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF), output per minute (CO), New York Heart Association (NYHA) cardiac function grade, QRS interval, ventricular electrode parameters and complications of the two groups were compared. Results There was significant difference in impedance between the two groups at the time of pacemaker implantation and one year after operation (P<0. 05) . There was significant difference in the perception between the two groups at the time of pacemaker implantation (P<0. 05), but there was no significant difference one year after operation (P>0. 05) . There was no significant difference in the threshold between the two groups at the time of pacemaker implantation and one year after operation (P>0. 05) . There were significant differences in LVEF, CO levels and QRS interval between the two groups one year after operation (P<0. 05), but there were no significant differences in LVEDD level and NYHA grade (P>0. 05) . There was no significant difference in the incidence of complications between the two groups (P>0. 05) . Conclusion HBP can significantly reduce the level of LVEDD, shorten the QRS interval and improve the left ventricular systolic function. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Computational Mechanics / Jisuan Lixue Xuebao is the property of Chinese Journal of Computational Mechanics Editorial Office, Dalian University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao is the property of Journal of Nanjing Agricultural University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To lay the foundation for hemophilia A gene therapy and real-time monitoring of human embryonic stem cells differentiation to endothelial cells, endothelial cell-specific promoters were cloned and its specificity was validated. Methods: A stable method of obtaining endothelial cells from umbilical vein was established. Then the expression of endothelial cell specific marker gene v WF and VE-cadherin were verified via RT-PCR and immunofluorescence. Promoter fragments of v WF and VE-cadherin gene were amplified using PCR. The EGFP expression vector originated from p EGFP-N1. The cloned promoters were substituted for the endogenous CMV promoter. The plasmids: pv WF-1, pv WF-2, p VE-1 and p VE-2 were constructed in total, which were then transfected into HUVECs and h ESCs. In order to find the strongest and most specific promoter, the expression of GFP 48 h after transfection were observed and compared. Results: Endothelial cells were isolated from umbilical vein and the markers of their specific genes were detectable. Four plasmids were confirmed by restriction enzyme digestion and sequencing. HUVECs and h ESCs were transfected by the constructed plasmids, and the GFP expression under fluorescence microscopy showed that p VE-2 promoter was of most specificity and transcription activity. Conclusions: An endothelial cell specific promoter with the highest GFP specificity and activity in endothelial cells was successfully cloned. [ABSTRACT FROM AUTHOR]