In recent years, deep eutectic solvents (DESs) have attracted increasing attention due to their ionic liquid analogue. Compared with ionic liquids, deep eutectic solvents had more advantages such as low-cost, easy synthesis and wide application. In the present work. deep eutectic solvents were prepared from choline chloride and ethylene glycol, choline chloride and 1.3-propanediol, choline chloride and 1,4-butanediol, respectively. At the same time their hydrogen bonding interactions were investigated by the FTIR and 'H NMR. The effects of different temperatures (303.15~343.15 K), molar ratio and alkyl chain length on the physicochemical properties of deep eutectic solvents were researched. The results showed that the hydrogen bonding of choline chloride is weakened with the increase of polyol content in choline chloride/alcohol deep eutectic solvent, and the hydrogen bonding of polyol is enhanced. It can form suitable hydrogen bonds when the choline chloride to ethylene glycol molar ratio of is 1: 3. Also, with the increasing of temperature, the viscosity of deep eutectic solvent notably decreases while the conductivity increases. On the other hand, density is slight affected by the temperature change. Under the same temperature, the viscosity and polarity of the deep eutectic solvent increases, and the conductivity, surface tension, and pH decreases, as the length of the alkyl chain increases. As glycol content increases, the viscosity decreases, and the pH increases first and then decreases, while the polarity and surface tension does not change significantly. The results of this study reveal the hydrogen bonding interaction of deep eutectic solvents based on choline chloride and different glycol. Furthermore, the physicochemical properties of DESs can provide scientific guidance for its application in the extraction field, which is of great significance and value. [ABSTRACT FROM AUTHOR]