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Copyright of Progress in Earthquake Sciences is the property of China Earthquake Administration, Institute of Geophysics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The thermal conductivity of thermal insulation materials is generally not greater than 0.2 W/(m • K). Because of its good thermal insulation performance-it is widely used in the field of building internal and external walls, roofs and thermal insulation mortar. Fiber reinforced composite thermal insulation foams with different fiber contents (0-3wt% -6wt% and 9wt%) were prepared with phenolic resin as matrix and chopped glass fiber as modified mate rial. The mechanical properties, micro morphology and thermal conductivity of composite thermal insulation foaming materials were analyzed by electronic universal testing machine, SEM and thermal conductivity tester. The results showed that with the increased of fiber content, the compressive strength and tensile strength of fiber-reinforced composite thermal insulation foaming materials increased first and then decreased. When the content of fiber was 6wt%, the compressive strength and tensile strength of composite thermal insulation foaming material reached the maximum, which were 0.241 and ().115 MPa respectively. With the increased of fiber content, the water absorption of composite thermal insulation foaming materials decreased first and then increased, when the content of fiber was 6wt%, the water absorption reached the minimum value of 27.9%. With the increased of fiber content, the brittleness of composite thermal insulation foaming material gradually decreased and the toughness gradually increased, when the content of fiber was 9wt%, the embrittlement rate of composite thermal insulation foaming material was the lowest, which was 1().2%. When the content of fiber was 6wt%, the minimum pore size of composite thermal insulation foaming material was about 3()()/.xm. when the fiber content increased to 9wt%, the number of pores tended to increased, and the size distribution was uneven, with large pores. With the increased of fiber content, the thermal conductivity of composite thermal insulation foams decreased first and then increased. when the content of fiber was 6wt%, the thermal conductivity of composite thermal insulation foaming material reached the lowest value of ().033 W / (m • K). The overall analysis showed that when the content of fiber was 6wt%, the comprehensive performance of fiber-reinforced composite thermal insulation foaming material was the best. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Computer Engineering & Applications is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To construct lentiviral vector carrying the rp-mt gene based on a ratiometric-pericam-mt (rp-mt) fluorescent protein modified by a circularly permuted yellow fluorescent protein (cpEYFP), verify the function of lentiviral vector in the mitochondrial calcium detection, and provide a reference for the further study on the role of mitochondrial calcium homeostasis in the cell life. Method: A recombinant lentivirus vector carrying the rp-mt gene was constructed with the lentivirus vector EF-1琢F-puro and the rp-mt plasmid, and was co-transfection with package plasmid in 293T cells. The constructed recombinant lentivirus was collected and infected the HCC cells SNU-739 in vitro. The function of recombinant lentivirus was validated. Results: Are combinant lentivirus vector carrying rp-mt gene with the lentivirus vector EF-1琢F-puro was successfully constructed, the titer of recombinant lentivirus was 4× 106TU/mL. More than 85% of the SNU-739 cells were observed to emit green fluorescence under a fluorescence microscope after infected by the recombinant lentivirus vector for 48 hours. The fluorescence located in the mitochondria of cells and was brighter or weaker as the variation of the concentration of calcium in mitochondria with Ru360 or CGP37157 treated of the cells. Conclusion: Are combinant lentivirus vector carrying rp-mt gene with the liposomes packing method was successfully constructed. The cell can stably express the fluorescigenic protein which located in the mitochondrial after infected by the recombinant lentivirus, and the calcium of the mitochondrial can be accurately detected. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the expression of ATPase family AAA Domain-containing protein 3A（ATAD3A）in colorectal cancer (CRC), and its influence on cell growth in CRC cell lines RKO and HCT116. Methods: 115 paired CRC tissues were collected to detect the expression of ATAD3A by immunochemistry staining. Lentivirus transfection and siRNA interference were used to overexpression and knockdown ATAD3A in CRC cells, and western blot was used to determine the ATAD3A expression in these cells. Cell proliferation was measured by MTS assay. Flow cytometry was used to detect the cell cycle and apoptosis. Results: The expression of ATAD3A in CRC was significantly upregulated than adjacent normal tissues (P＜0.001). Overexpression of ATAD3A in RKO and HCT116 significantly promoted cell proliferation, upregulated the S phase fractions and reduced cell apoptosis. In contrast, knockdown ATAD3A in CRC cells obviously inhibited cell proliferation, G1 phase arrest and enhanced cell apoptosis. Conclusion: ATAD3A was frequently upregulated in CRC tissues, and promoted CRC cell proliferation, advanced cell cycle procession and inhibited cell apoptosis. [ABSTRACT FROM AUTHOR]

Copyright of Petroleum Refinery Engineering is the property of Petroleum Refinery Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)