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Objective Network pharmacology, molecular docking, metabolomics and experimental verification were used to investigate the effect of Gegen Decoction on epileptic seizure under cerebral hypoxia. Methods The core targets of Gegen Decoction, epilepsy and cerebral hypoxia were screened by network pharmacology, and the correlation between ligand and receptor was verified by molecular docking. Further animal experiments were carried out to verify the effect of Gegen Decoction on the rat model with epilepsy accompanied by cerebral hypoxia, which was established by lithium chlorine-pilocarpine method combined with unilateral common carotid artery ligation and placing in a closed chamber with oxygen content of 10%-15%. Sixty rats were randomly divided into blank group, model group, phenytoin sodium (26 g.kg-1) group, high, medium and low-dose (10.62, 5.31, 2.655 g.kg-1) Gegen Decoction groups. After treatment, the number of epileptic seizures rats in each group was counted, and the recovery of nerve function and epileptic seizures were evaluated. HE staining was used to observe the morphological changes of the CAl region of hippocampus in rats. The expression of interleukin-6 (IL-6) and interleukin-17 (IL-17) were detected by immunohistochemistry. The changes of brain neurotransmitters were analyzed by targeted metabolomics. Results Network pharmacological results suggested that 128 active components and 228 targets were obtained. A PPI network map of drug-disease was constructed by STRING. The results showed that STAT3, IL-6 and AKTI were the core targets. GO enrichment for biological processes (BP) involves responses to external irritant, oxygen levels, hypoxia and metabolic processes of reactive oxygen species. Cell components (CC) mainly involve in the impact on cell membrane and protein kinase complex, while molecular function (MF) involves in influence on the binding of ubiquitin-like protein and ubiquitin-like protein ligase. The key pathways of Gegen Decoction in the treatment of hypoxic-induced epilepsy in the brain, which were analyzed by KEGG, involved lipid and atherosclerosis signaling pathway, IL-17 signaling pathway, Th-17 signaling pathway and so on. The experimental results showed that the number of seizures in the Gegen Decoction group was significantly decreased compared with that in the model group (P<0.01), the morphology and structure of hippocampal cells were improved to varying degrees, and cell vacuolation was reduced. The expression of IL-17A and IL-6 in all treatment groups was lower than those in model group, and there was statistical significance in medium-dose Gegen Decoction group (P<0.01, P<0.05). Brain metabolomics test showed that compared with model group, monoamine neurotransmitters including 5-hydroxytryptophan (5-HTP) and norepinephrine (NE) were increased in high-dose Gegen Decoction group, while tryptophan metabolite kynurenine (Kyn) was decreased. Conclusion Gegen Decoction can reduce the levels of serum inflammatory factors IL-6 and IL-17A in epileptic rats under hypoxia. Moreover, Gegen Decoction can reduce epileptic seizures by improving hypoxia-induced inflammatory response, increasing the content of monoamine neurotransmitter (5-HTP and NE), as well as reducing the content of tryptophan metabolite Kyn and the toxin of tryptophan metabolism pathway. [ABSTRACT FROM AUTHOR]

Epilepsy is a kind of brain dysfunction caused by abnormal synchronous discharge of brain neurons, and is one of the common diseases of the nervous system. Patients need to take anti-epileptic drugs regularly for a long time to achieve seizures-free. For pregnant women, antiepileptic drugs can enter the fetus through the placental barrier causing adverse effects. Lamotrigine and levetiracetam have the lowest potential teratogenic risk, oxcarbazepine has a low risk, topiramate has high risk, while valproic acid has the most severe teratogenic effect and negative effects on the neuropsychological development of children exposed in utero, and should be avoided as far as possible. At present, the management of antiepileptic drugs is still a difficult clinical problem. Pre-pregnancy preparation and rational decision-making of antiepileptic drugs usage during pregnancy are very important. [ABSTRACT FROM AUTHOR]

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Objective: To investigate the relationship between serum neurofilament light chain protein (NFL), homocysteine (Hcy), irisin (Irisin) and cognitive function and seizure control after treatment in patients with epilepsy. Methods: 196 patients with epilepsy admitted to The Affiliated Hospital of Xuzhou Medical University from February 2021 to February 2023 were selected,according to the evaluation results of the Montreal Cognitive Assessment Scale (MoCA), patients were divided into cognitive impairment group and non-cognitive impairment group, with 70 cases and 126 cases respectively. All patients with epilepsy were treated with antiepileptic drugs, the levels of serum NFL, Hcy and Irisin were detected before treatment, the seizure control was evaluated 6 months after treatment, the patients were divided into complete control group (139 cases) and incomplete control group (57cases) according to this. The factors affecting the incomplete control of seizures in patients with epilepsy after treatment were analyzed by multivariate Logistic regression analysis. Results: The level of Irisin in cognitive impairment group was lower than that in non-cognitive impairment group, and the levels of serum NFL and Hcy were higher than those in non-cognitive impairment group (P＜0.05). The levels of serum NFL and Hcy in incomplete control group were higher than those in incomplete control group (P＜0.05), and the level of Irisin was lower than that in incomplete control group (P＜0.05). Electroencephalogram abnormalities, generalized seizures, high NFL and Hcy were risk factors for incomplete control after antiepileptic treatment in patients with epilepsy (P＜0.05), and high Irisin was a protective factor (P＜0.05). Conclusion: The levels of serum NFL and Hcy in patients with epilepsy and cognitive impairment are increase, and the level of Irisin is decrease, which are relate to the control of seizures after antiepileptic treatment. [ABSTRACT FROM AUTHOR]

Objective To master the information of patients with convulsion epilepsy in phenobarbital in rural epilepsy prevention and management project of Heilongjiang Province - understand the reasons for the withdrawal, analyze the influencing factors, and provide reference for improving the patient retention rate and work quality. Methods From 2011 to 2020, 3 890 convulsion epilepsy patients received phenobarbital treatment in Heilongjiang Province, of which 807 patients were enrolled. For 807 patients, EpiData, EXCEL and SPSS 19. 0 software were used for data entry, processing and statistical analysis of the influencing factors in the withdrawal group, including analysis of variance, univariate analysis, and multivariate survival analysis. Results 3 890 patients with convulsive epilepsy enrolled in phenobarbital treatment group in rural epilepsy project of Heilongjiang Province, 807 cases dropped out successively, the dropout rate was 20.75%, the primary factors contributing to dropout were relocation, noncompliance with medication, and self - perceived or actual remission. Multivariate Cox regression analysis showed that female patients (HR = 1. 278, 95% CI: 1. 112 - 1. 468) were the risk factors for dropping out, and the probability of dropping out in female patients was 1. 278 times that of male patients. Conclusion The withdrawal rate of phenobarbital epilepsy treatment in rural areas of Heilongjiang Province is relatively high. In the later management work, the publicity and education of epilepsy prevention and treatment knowledge should be strengthened to provide personalized treatment programs and improve the treatment efficiency, especially for female epilepsy patients. [ABSTRACT FROM AUTHOR]

Objective: To investigate whether quercetin reduces neuroinflammation in epileptic rats by regulating high mobility group protein B1 （HMGB1）, receptor for advanced glycation end products （RAGE） and nuclear factor kappa-B （NF-κB）, and to explore the possibility of HMGB1/RAGE/NF-κB pathway as a new target of quercetin. Methods: Twelve SD rats were randomly selected from 60 SD rats as healthy group, and the remaining rats were used to construct experimental models of epilepsy rats. Rats that were successfully modeled were divided into model group, high-dose quercetin group, low-dose quercetin group, quercetin+pathway activator group, with 12 rats in each group. Pathological changes of hippocampal tissue of rats were observed; neurobehavioral function of rats was evaluated; levels of tumor necrosis factor-α （TNF-α）, IL-6 and IL-1β, and mRNA and protein expression levels of HMGB1, RAGE, NF-κB in hippocampal tissue of rats were detected. Results: Structure of hippocampus of rats in healthy group was complete; compared with healthy group, structure of hippocampal tissue of rats in model group was scattered, the number of surviving neurons was observably reduced, apoptotic index was observably increased, the Racine grade was observably increased, TNF-α, IL-1β, IL-6 contents and mRNA and protein expression levels of HMGB1, RAGE, NF-κB in hippocampal tissue were significantly increased; compared with model group, structure of hippocampal tissue of rats in low- and high-dose quercetin groups was relatively complete, the number of surviving neurons was observably increased, apoptotic index was observably decreased, the Racine grade was significantly decreased, TNF-α, IL-1β, IL-6 contents and mRNA and protein expression levels of HMGB1, RAGE, NF-κB were significantly reduced, and the improvement effect of high-dose quercetin group was better; compared with high-dose quercetin group, the number of surviving neurons in quercetin+pathway activator group was significantly reduced, apoptotic index was significantly increased, the Racine grade was significantly increased, TNF-α, IL-1β, IL-6 contents and mRNA and protein expression levels of HMGB1, RAGE, NF-κB in hippocampal tissue were significantly increased. Conclusion: Quercetin can effectively reduce neuroinflammation in epileptic rats by inhibiting the HMGB1/RAGE/NF-κB pathway and reducing the mRNA and protein expressions of related genes. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the correlation between the polymorphism of glutamate receptor ionized NMDA1 receptor gene (GRINI) and the susceptibility and drug resistance of epilepsy in Han population in western Guangdong. Methods A total of 200 with epilepsy patients (case group) and 200 healthy adults (control group) were included. Peripheral venous blood was extracted to extract whole genome DNA, and the gene polymorphisms of rs2301363 and rs11146020 loci of GRIN1 gene were detected. The case group was further divided into the drug resistant group (52 cases) and the non-drug resistant group (148 cases). The differences in genotype and allele frequency distribution of rs2301363 and rs11146020 loci of GRINI gene were compared among the groups. Results There were significant differences in the genotype and allele frequency distribution of GRINI gene rs2301363 and rs11146020 between the case group and the control group (P< 0.05). The frequency of TT and CT genotypes of GRINI gene rs2301363 locus in the case group was significantly higher than that in the control group. Compared with CC genotype, TT and CT genotypes increased the risk of epilepsy (OR>1). The frequencies of AG and AA genotypes at rs11146020 locus of GRINI gene in the case group were significantly higher than those in the control group. Compared with GG genotype, AG and AA genotypes increased the the risk of epilepsy (OR>1). There were no significant difference in the distribution of genotypes and allele frequencies of GRINI gene between the drug resistant and the non-drug resistant groups (P>0.05). Conclusion The polymorphisms of rs2301363 and rs11146020 in GRINI gene are related to the susceptibility to epilepsy, but not to the drug resistance of epilepsy. [ABSTRACT FROM AUTHOR]

Objective To observe the changes in the expression levels of serum LncRNA-ZFAS1 and miR-15a-5p in patients with status epilepticus (SE), and to analyze the predictive efficacy of the relative expression of serum LncRNAZFAS1 and miR-15a-5p for the poor prognosis of SE patients. Methods Totally 100 cases of SE patients (SE group) and 68 cases of healthy people with physical examination in the same period (control group) were selected, and real-time fluorescence quantitative polymerase chain reaction was used to detect serum LncRNA-ZFAS1 and miR-15a-5p levels in the subjects, and the binding sites of LncRNA-ZFAS1 and miR-15a-5p were predicted by starBase database. General data such as gender, age, disease duration, seizure type, underlying disease, in-hospital complications, brain tissue abnormalities, cerebrospinal fluid abnormalities, electroencephalogram abnormalities, concomitant fever, history of ≥2 previous seizures, duration of epilepsy, mechanical ventilation, status epilepticus severity score (STESS), time in the intensive care unit, length of hospital stay, and types of therapeutic medications were collected from the patients with SE. According to the Glasgow Prognostic Score, the SE patients were divided into the poor prognosis group of 31 cases and the good prognosis group of 69 cases. We compared the general data and the relative expression levels of serum LncRNA-ZFAS1 and miR-15a-5p of the patients in the poor prognosis group and the good prognosis group. Risk factors for poor prognosis in SE patients were analyzed by multifactorial Logistic regression analysis. Using poor prognosis in SE patients as the dependent variable, and variables that were statistically different in univariate analyses as independent variables. The risk factors for poor prognosis in SE patients were analyzed using multivariate Logistic regression analysis. The predictive efficacy of the combination of relative expression of serum LncRNA-ZFAS1 and miR-15a-5p on poor prognosis in SE patients was analyzed using receiver operating characteristic (ROC) curve. Results The relative expression of serum LncRNA-ZFAS1 in the SE group was higher than that of the control group, and the relative expression of miR-15a-5p was lower than that of the control group (both P<0. 05) . There was a negative correlation between the relative expression of serum LncRNA-ZFAS1 and miR-15a-5p in the SE patients (r＝–0. 742，P<0. 05) . LncRNA-ZFAS1 had a complementary sequence to the 3' untranslated end of miR-15a-5p. The convulsive SE, seizure duration ≥1 h, the proportion of mechanical ventilation, STESS score, and serum LncRNA-ZFAS1 were higher in the poor prognosis group than in the good prognosis group, and the relative expression of serum miR-15a-5p was lower than that of the good prognosis group (all P<0. 05) . The AUC of serum LncRNA-ZFAS1 in predicting poor prognosis of SE patients was 0. 786, with a sensitivity of 67. 74% and a specificity of 75. 36%； the AUC of serum miR-15a-5p in predicting poor prognosis of SE patients was 0. 781, with a sensitivity of 90. 32% and a specificity of 53. 62%； and the AUC of serum LncRNA-ZFAS1 combined with miR-15a-5p in predicting poor prognosis of SE patients was 0. 879, with the sensitivity of 87. 10% and specificity of 73. 91%. Conclusions Serum LncRNA-ZFAS1 is highly expressed and miR-15a-5p is low expressed in SE patients, which shows a negative correlation. Elevated relative expression of serum LncRNA-ZFAS1 is an independent risk factor for poor prognosis of SE patients, and elevated relative expression of serum miR-15a-5p is an independent protective factor. Detection of serum LncRNAZFAS1 and miR-15a-5p can be used for the prediction of poor prognosis in SE patients, and the predictive value of the two combined is higher [ABSTRACT FROM AUTHOR]

Objective: To investigate the impacts of miR-125a-5p on neurological dysfunction and inflammatory response in pentylenetetrazole （PTZ）-induced epileptic rats by targeting signal transducer and activator of transcription 3 （STAT3）. Methods: Rats epilepsy models were established by PTZ ignition method and randomly grouped into six groups: Con group， PTZ group， PTZ+ agomir NC group， PTZ+miR-125a-5p agomir group， PTZ+miR-125a-5p agomir+pcDNA group and PTZ+miR-125a-5p agomir+pc- STAT3 group. Impacts of miR-125a-5p on epilepsy duration， latency， number and severity of seizures in rats were evaluated. qRTPCR was applied to detect miR-125a-5p and STAT3 mRNA expressions； Modified Nervous System Severity Score （mNSS）， open field test and Rotarod test were applied to evaluate neurological function in epileptic rats； Morris water maze test was applied to evaluate cognitive function of epileptic rats； immunostaining was applied to observe neuronal apoptosis in hippocampal CA1 and CA3 regions； ELISA was applied to detect levels of inflammatory factors （TNF-α， IL-6， IL-1β and IL-18） in hippocampus； Western blot was applied to detect STAT3 protein expression in hippocampus； dual-luciferase assay was applied to verify the relationship between miR-125a-5p and STAT3. Results: In PTZ-induced epilepsy rats， expression of miR-125a-5p was up-regulated， while expression of STAT3 was down-regulated， and miR-125a-5p was targeted and inhibited expression of STAT3. Compared with PTZ group， PTZ+miR-125a-5p agomir group had shorter latency， lower epilepsy score， fewer seizure duration and times， lower mNSS， increased fall latency and movement distance in peripheral areas and total open area， greatly decreased escape latency， increased number of platform crossings and time spent in the target quadrant and swimming distance， reduced expressions of TNF-α， IL-6， IL-1β， IL-18 in hippocampus， and increased NenN+ cells in CA3 and CA1 regions （P<0.05）； overexpression of STAT3 could reverse the alleviating effects of miR- 125a-5p agomir on neurological dysfunction and inflammatory response in epileptic rats （P<0.05）. Conclusion: miR-125a-5p attenu-ates neurological dysfunction and inflammatory response in PTZ-induced epilepsy rats by targeting down-regulation of STAT3. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between serum neuromodulin-1 (NRG1), fibrinogen-like protein 2 (FGL2), fibroblast growth factor 9 (FGF9) and epilepsy after acute ischemic stroke (AIS) and its predictive value. Methods: 450 AIS patients admitted to Longquan Hospital of Sichuan University West China Hospital from January 2021 to March 2022 were selected, and the patients were divided into epilepsy group(n=41) and non-epilepsy group(n=409) according to whether epilepsy occurred after 1 year of follow-up. The levels of serum NRG1, FGL2 and FGF9 were detected by enzyme-linked immunosorbent assay(ELISA). The factors affecting epilepsy after AIS were analyzed by multivariate Logistic regression analysis, and the predictive value of serum NRG1, FGL2 and FGF9 levels for epilepsy after AIS were analyzed by receiver operating characteristic(ROC) curve. Results: Compared with non-epileptic group, the serum levels of NRG1 and FGL2 in epileptic group increased, and the FGF9 level decreased, the difference was statistically significant(P<0.05). Multivariate Logistic regression analysis showed that anterior circulation infarction, the increased infarct area, hemorrhagic transformation, the increase of National Institute of Health stroke scale(NIHSS) score, NRG1 and FGL2 were independent risk factors for epilepsy after AIS, and the increase of FGF9 was an independent protective factor(P<0.05). ROC curve analysis showed that the area under the curve of serum NRG1, FGL2 and FGF9 levels combined to predict epilepsy after AIS was 0.921, which was greater than 0.789,0.779 and 0.795 predicted by serum NRG1, FGL2 and FGF9 levels alone. Conclusion: The increased serum NRG1 and FGL2 levels and decreased FGF9 levels are closely associated with epilepsy after AIS, and the combined detection of serum NRG1, FGL2, and FGF9 levels is of high value in predicting epilepsy after AIS. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of P2X7 receptor (P2X7R) inhibitor on kainic acid-induced epilep-togenesis and hippocampal damage in mice. Methods Ninety-six male C57BL/6 mice were randomly divided into the control group of 36, the model group of 36, and the P2X7R inhibitor group of 24. Mice in the P2X7R inhibitor group were injected intraperitoneally with A438079, a P2X7R-specific inhibitor, 30 min before modeling, and mice in the model and control groups were injected with the equal volume of normal saline at the same time point. The epilepsy model was estab⁃ lished by intraperitoneal injection of kainic acid in the model group and the P2X7R inhibitor group, and an equal volume of normal saline was injected intraperitoneally in mice of the control group. The severity of seizures was assessed according to the electroencephalography (EEG), Western blotting was used to determine the level of hippocampal P2X7R expression, and Nissl staining was used to assess the extent of hippocampal damage in the CA1 region. Anxiety-like behaviors of mice in the control and model groups were observed in the open-field test. If anxiety-like behaviors existed in the model group, the model group was further divided into the anxiety control subgroup and P2X7R-inhibited subgroup; mice in the P2X7Rinhibited subgroup were injected with A438079 intraperitoneally every day, and mice in the anxiety control subgroup were injected with an equal volume of normal saline every day intraperitoneally, and open-field test was conducted again after 14 days. The morris water maze was used to observe the cognitive function of the mice, and if there was cognitive impairment in the model group, the model group was further divided into the cognitive control subgroup and P2X7R-inhibited subgroup. Mice in the P2X7R-inhibited subgroup were injected intraperitoneally with 15 mg/kg of A438079 every day, while mice in the cognitive control subgroup were injected with the equal volume of normal saline every day, and then the morris water maze was performed again after 14 days. Results The EEG did not show any epileptiform activity in the control group. Mice in the model group developed status epilepticus, in which EEG showed strong discharge power and high ampli⁃ tude. Seizures occurred in the P2X7R inhibitor group, but the power and amplitude of EEG were attenuated compared with those in the model group; total power and mean amplitude were as follows： the model group > P2X7R inhibitor group > con⁃ trol group (both P<0. 05), The expression of P2X7R protein in the hippocampal tissues was higher than that in the control group, and the expression of P2X7R protein was lower in the P2X7R inhibitor group than in the model group (both P< 0. 05), The results of nissl staining showed that the structure of hippocampal CA1 region was intact, the number of surviving neuronal cells was large, and the nissl body was big and its number was large in the control group; the structural integrity of hippocampal CA1 region was disrupted, the number of neuronal cells was small, and the number of nissl body decreased in the model group; the structure of the hippocampal CA1 region, the number of neuronal cells, and the number of nissl body in the P2X7R inhibitor group improved in comparison with those in the model group. The results of the open-field test showed that the number of entering the central region and the length of stay in the central region were less in the model group than in the control group, and the number of entering the central region and the length of stay in the central region were less in the anxiety control subgroup than in the P2X7R-inhibited subgroup (all P<0. 05), The results of the morris water maze showed that there were no statistically significant differences in the number of platform crossings or the length of stay in target quadrant between the model and control groups (all P>0. 05), Conclusion P2X7R inhibitor reduces the severity and frequency of kainic acid-induced seizures, ameliorates epilepsy-induced hippocampal damage, and alleviates anxiety-like behavior in epileptic mice. [ABSTRACT FROM AUTHOR]

目的 观察SCN2A基因突变相关癲痫患儿外周血miRNA-15a-5p水平变化, 分析外周血miRNA-15a-5p相 对表达量与 SCN2A基因突变相关癲痫患儿病情的相关性。方法 纳入同期住院癫痫患儿 20例, 其中 SCN2A基因 突变的癫痫患儿10例 (突变组)、非SCN2A基因突变的癫痫患儿 (非突变组)10例；突变组中, 癫痫控制良好4例、难 治性癫痫6例；SCN2A基因突变位点在钠离子通道的关键区6例、连接区4例。另择同期体检健康儿童10例作为对 照组。采用qRT-PCR检测上述三组外周血miRNA-15a-5p。采用Spearman相关分析外周血miRNA-15a-5p水平与癫 痫患儿病情的相关性。结果 与非突变组和对照组相比, 突变组患儿外周血 miRNA-15a-5p 相对表达量升高 (P< 0. 001)；在突变组中, 与SCN2A基因突变位点在钠离子通道的连接区患儿相比, 在关键区的患儿外周血miRNA-15a-5p 表达升高, 且表达量与突变位点是否在关键区相关 (r=0. 71, P<0. 05)。与癫痫控制良好患儿相比, 难治性癫痫患儿 外周血miRNA-15a-5p相对表达量升高, 且表达量与患儿病情相关 (r=0. 85, P<0. 05)。结论 SCN2A基因突变相关 癫痫患儿外周血中miRNA-15a-5p相对表达量升高, 其表达量高的癫痫患儿病情重。 [ABSTRACT FROM AUTHOR]

Functional near-infrared spectroscopy (fNIRS) is widely used in neurology and epilepsy is one of them. Combined with the electroencephalogram (EEG), fNIRS could record the whole state of seizure, which is beneficial to the understanding of the pathophysiological mechanism of epilepsy. In addition, fNIRS contributes to the diagnosis and treatment of epilepsy with the assistance of the epileptic focus localization and the evaluation of lateralization of language function. This paper aims to review the application and research progress of fNIRS in epilepsy so as to provide the reference for researchers. [ABSTRACT FROM AUTHOR]

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Objective: To investigate the changes in peripheral blood T lymphocyte subsets and cytokines in drug-resistant epilepsy patients and their relationship with cognitive function and prognosis. Methods: 100 drug-resistant epilepsy patients admitted to our hospital from April 2022 to May 2023 were selected and divided into drug-resistant epilepsy group (45 cases) and drug-sensitive epilepsy group (55 cases) based on drug sensitivity. 50 healthy individuals who underwent physical examination during the same period were selected as the control group. Using the Montreal Cognitive Function Assessment Scale (MoCA), Clinical Memory Scale (MQ), and Number Symbol Conversion Test to assess overall intelligence, memory function, and attention in patients, while also detecting the distribution of peripheral blood T lymphocyte subpopulations and interleukin-1 in patients β(IL-1β), IL-6 and tumor necrosis factor α (TNF-α) levels. The correlation between cognitive function indicators, T cell subpopulations, and cytokines in drug-resistant epilepsy patients was analyzed using Spearman correlation analysis. Results: (1) The MoCA score, MQ and DST scores of the drug resistant and drug sensitive groups were lower than those of the control group (P<0.05), while the MoCA score, MQ and DST scores of the drug resistant group were lower than those of the drug sensitive group (P<0.05); (2) The percentages of CD4+ and CD8+ in peripheral blood of the drug resistant and drug sensitive groups were lower than those of the control group, the levels of IL-1β、IL-6 and TNF-α The levels were higher than those of the control group(P<0.05), and the percentages of CD4+ and CD8+ in peripheral blood of the drug resistant group were lower than those of the drug sensitive group, the levels of IL-1β, IL-6 and TNF-α were higher than those of the drug sensitive group(P<0.05); (3) Spearman analysis results showed that the distribution of CD4+ cells in peripheral blood was significantly positively correlated with MoCA score, MQ and DST score in drug-resistant epilepsy patients, while IL-1β was negatively correlated with MoCA score, MQ and DST score, IL-6 and TNF-α were negatively correlated with MoCA score and MQ score(P<0.05). The distribution of CD8+ cells was not significantly correlated with MoCA score, MQ and DST score, as well as IL-6 and TNF-α with DST score in drug-resistant epilepsy patients(P>0.05); (4) The proportion of CD4+ and CD8+ cells in peripheral blood of drug-resistant epilepsy patients with poor prognosis was lower than that of patients with good prognosis, the levels of IL-1β, IL-6 and TNF-α were higher than that of patients with good prognosis(P<0.05). Conclusion: Patients with drug-resistant epilepsy exhibit abnormal distribution of T lymphocyte subpopulations and cytokine expression, which are associated with decreased intelligence, memory function, and attention. [ABSTRACT FROM AUTHOR]

癫痫是世界卫生组织重点防治的五大神经精神疾病之一。2017年6月至今，我国国家神经系统疾病医疗质量控制中心癫痫质控专病组在充分参考国内外循证证据及既往经验的基础上，经过3批试点，逐步启动全国癫痫医疗质量控制指标体系工作，构建了覆盖我国31个省级行政区，130家质量控制哨点医院的国家癫痫及惊厥性癫痫持续状态医疗质量控制体系，并依托哨点医院反馈的诊疗指标数据，对既往医疗质量控制指标体系进行修订，最终纳入20个经过验证的癫痫与惊厥性癫痫持续状态医疗质量控制指标。2020年，国家卫生健康委员会办公厅发布了该指标体系。单病种医疗质量控制指标依托全国质量控制网络体系落实医疗质量控制与管理，旨在改进医疗质量、促进病种诊疗同质化。目前该指标体系已被纳入三级医院评审标准，在持续加强指标信息培训、分析和反馈的同时，不断推进各级医疗机构癫痫和惊厥性癫痫持续状态的规范诊治。 Abstract: Epilepsy is one of the five major neuropsychiatric diseases that the World Health Organization (WHO) focuses on prevention and treatment. From June 2017 to the present, the Epilepsy Working Group of National Center for Healthcare Quality Management in Neurological Diseases has launched the national epilepsy medical quality indicator system after three batches of pilots, based on full reference to domestic and overseas evidence-based evidence and previous indicator experience. It has built a medical quality control system for epilepsy and convulsive status epilepticus covering 130 sentinel hospitals in 31 provincial administrative regions across China. Regarding on the diagnosis and treatment indicator data feedback from sentinel hospitals, the system was revised based on the published quality control indicators. Twenty validated indicators in epilepsy and convulsive status epilepticus were included. In 2020, the General Office of the National Health Commission issued the indicator system. Disease quality control indicators rely on the national quality control network system to implement medical quality control and management, aiming at improving medical quality and promoting the homogenization of diagnosis and treatment. Currently, this indicator system has been incorporated into the accreditation standards for tertiary hospitals. In addition to strengthening the training, analysis and feedback of information on the indicators, the standardized diagnosis and treatment of epilepsy and convulsive status epilepticus in medical institutions at all levels are constantly promoted.

Objective To explore the status and influencing factors of psychological resilience in epilepsy patients. Methods A total of 80 patients with epilepsy admitted to the hospital from June 2021 to December 2022 were selected. The Connor-Davidson resilience scale（CD-RISC） was used to evaluate their psychological resilience. The baseline data questionnaire was designed, and the clinical data of the patients were counted in detail. The CD-RISC scores of patients with different characteristics were compared and the influencing factors of psychological resilience of patients with epilepsy were analyzed. Results The average CD-RISC score of 80 patients was（59. 55±7. 14）scores. There were significant differences in CD-RISC scores among epilepsy patients with different seizure frequency, age, social support, family care degree and education level（P＜0. 05）. There were no significant differences in CD-RISC scores among epilepsy patients with different disease course, family history of epilepsy, family monthly per capita income, gender, combined basic diseases and marital status（P＞0. 05）. The influencing factors of psychological resilience of epilepsy patients were young age, low family care, high seizures frequency, low education level and low social support. Among them, age had the greatest impact on psychological resilience of epilepsy patients, and education level had the least impact on psychological resilience of epilepsy patients. The differences were statistically significant（P＜0. 05）. Conclusion The level of psychological resilience in patients with epilepsy is low, which may be related to age, seizure frequency, education level and family care. [ABSTRACT FROM AUTHOR]

儿童继发性枕叶癫痫常伴有脑组织结构异常, 经癫痫术前评估后, 一般采用切除或离断致癫相关脑组织的治疗方法。近年来, 随着立体脑电图(stereo electroencephalogram, SEEG)植入技术在国内的开展, 通过收集患儿癫痫发作症状学资料、视频脑电图和影像学资料, 经分析讨论后植入SEEG监测, 明确致癫灶及放电传播网络, 进而在SEEG引导下行射频热凝(radiofrequency thermocoagulation, RF-TC)治疗, 可获得满意疗效。本文报道1例儿童继发性枕叶癫痫的微创诊治经验。 [ABSTRACT FROM AUTHOR]

Epilepsy is one of the five major neuropsychiatric diseases that the World Health Organization (WHO) focuses on prevention and treatment. From June 2017 to the present, the Epilepsy Working Group of National Center for Healthcare Quality Management in Neurological Diseases has launched the national epilepsy medical quality indicator system after three batches of pilots, based on full reference to domestic and overseas evidence-based evidence and previous indicator experience. It has built a medical quality control system for epilepsy and convulsive status epilepticus covering 130 sentinel hospitals in 31 provincial administrative regions across China. Regarding on the diagnosis and treatment indicator data feedback from sentinel hospitals, the system was revised based on the published quality control indicators. Twenty validated indicators in epilepsy and convulsive status epilepticus were included. In 2020, the General Office of the National Health Commission issued the indicator system. Disease quality control indicators rely on the national quality control network system to implement medical quality control and management, aiming at improving medical quality and promoting the homogenization of diagnosis and treatment. Currently, this indicator system has been incorporated into the accreditation standards for tertiary hospitals. In addition to strengthening the training, analysis and feedback of information on the indicators, the standardized diagnosis and treatment of epilepsy and convulsive status epilepticus in medical institutions at all levels are constantly promoted. [ABSTRACT FROM AUTHOR]

BACKGROUND: The mechanism mining of Alzheimer’s disease is very important and bioinformatics is an effective prediction technology for the potential targets of Alzheimer’s disease. OBJECTIVE: To screen the genes related to the pathogenesis of Alzheimer’s disease and verify them at the animal level by bioinformatics analysis. METHODS: Differentially expressed genes were screened through the GEO online database. GO/KEGG enrichment analysis was performed using the DAVID online database. A protein-protein interaction network was constructed using the STRING database. Target protein distribution in human was identified using the HPA database. Finally, protein expression was analyzed using immunofluorescence and western blot techniques. RESULTS AND CONCLUSION: Gene chips for Alzheimer’s disease and healthy populations were obtained using GSE48350, a dataset within the GEO online database, and the GEO2R online analysis platform was used to analyze the differentially expressed genes between the two populations, including 42 upregulated genes and 131 down-regulated genes. Results of the GO enrichment analysis suggested that the differentially expressed genes were mainly located in presynaptic, transport vesicles and extracellular vesicles, to mediate biological processes such as neurotransmitter release and synaptic vesicle function. KEGG pathway analysis results showed that differentially expressed genes were mainly enriched in synaptic function-related signaling pathways such as neuroactive ligand-receptor interactions, γ-aminobutyric acid synapses and retrograde endogenous cannabinoid signaling. Based on the protein interaction network, five up-regulated hub genes (CLU, GFAP, CD44, FOS, ANXA1) and five down-regulated hub genes (GABRG2, SYT1, SYN2, KCNC1, SLC32A1) were identified. It was confirmed that the expression of GABRG2, KCNC1, and SLC32A1 in the hippocampal tissue in a mouse model of Alzheimer’s disease were significantly downregulated. To conclude, these three genes can be used as potential genes for the treatment and diagnosis of Alzheimer’s disease and provide important clues for the treatment of Alzheimer’s disease. GABRG2, KCNC1 and SLC32A1 are also co-therapeutic targets for Alzheimer’s disease and epilepsy. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

癫痫是世界卫生组织重点防治的五大神经精神疾病之一，在我国有超1000万人受累，疾病负担沉重。经规范治疗，70%以上的癫痫患者可达到控制发作、重返社会的治疗目标。提高癫痫诊疗质量可有效改善我国癫痫医疗的可及性。2017年，在国家卫生健康委员会的支持下，国家神经系统疾病医疗质量控制中心成立了癫痫质控专病组，启动了癫痫及惊厥性癫痫持续状态医疗质量控制体系建设。癫痫及惊厥性癫痫持续状态医疗质量控制体系建设强化了我国的癫痫诊疗体系，调查了我国癫痫诊治现状，建设了数据来源互补医疗质量体系动态反馈机制。应用癫痫及惊厥性癫痫持续状态医疗质量工具可持续推动我国癫痫诊疗质量的同质化发展。 Abstract: Epilepsy is one of the five major neuropsychiatric diseases that the World Health Organization focuses on. There are more than 10 million people with epilepsy in China, which lead to tremendous burden of disease. Fortunately, over 70% of epilepsy patients can achieve the treatment goal of seizure control and reintegration into society under standardized treatment. Improving the qualityof diagnosis and treatment of epilepsy can effectively improve the accessibility of medical care in people with epilepsy in China. In 2017, with the support of the National Health Commission, the National Center for Healthcare Quality Management in Neurological Diseases set up the Epilepsy Medical Working Group to start the construction of the medical quality control system in epilepsy and convulsive status epilepticus. The construction of medical quality control system for epilepsy and convulsive status epilepticus strengthened the national epilepsy management system, found out the status of diagnosis and treatment in China, built a dynamic feedback mechanism for the medical quality system with multiple data sources complementing each other. Applying medical quality tools for epilepsy and convulsive status epilepticus, which have continued to promote the homogenization of epilepsy diagnosis and treatment in China.

青少年多形性低级别神经上皮肿瘤(PLNTY)是-种罕见的惰性脑肿瘤. 本文报道2例PLNTY 病例,主要临 床影像及病理表现为与癫痫发作强相关、弥漫性生长模式、频繁出现少突胶质细胞瘤样成分、特征性钙化、CD34(+)和丝裂原 活化蛋白激酶通路激活基因异常. 本文旨在探讨PLNTY的临床表现、影像学、病理及免疫组化特点,以提高对该病的诊断. [ABSTRACT FROM AUTHOR]

儿童和青少年疫苗接种是预防和控制传染性疾病的有效策略。然而，临床报告显示，该年龄阶段疫苗接种可能与癫痫或癫痫发作有关，因而可能会增加公众对疫苗接种的忧虑意识，引起“疫苗犹豫”，降低疫苗接种的覆盖率。目前，尚没有研究证据表明疫苗接种影响儿童和青少年神经发育及诱发癫痫或癫痫发作。在本综述中，我们探讨了儿童和青少年常见疫苗如百日咳疫苗、麻疹-腮腺炎-风疹疫苗、新型冠状病毒疫苗、乙型肝炎疫苗、轮状病毒疫苗等多种疫苗与癫痫或癫痫发作的相关性分析，综合归纳分析结果，为正确认识疫苗接种的安全性，提高我国疫苗覆盖率提供科学依据。

Objective To explore the application effect of diversified health education inpatients with brain tumor-related epilepsy and their family members . Methods A total of 84 patients with brain tumor-related epilepsy and their family members admitted to hospital from January 2019 to December 2021 were selected as the research objects. According to the order of admission, patients were divided into control group and experimental group, with 42 cases in each group. The control group received routine health education, and the experimental group received diversified health education. The knowledge of epilepsy disease, medication compliance and family caregivers’ first aid skills of the two groups were compared. Results After intervention, the scores of disease cognition assessment and rate of medication compliance in the experimental group were higher than those in the control group ( P＜0. 05 ). The score of first aid skills assessment in the experimental group was higher than that in the control group, with a significant difference(P＜0. 01). Conclusion Diversified health education is conducive to improving the cognitive level of epilepsy knowledge and medication compliance of patients, and improving the epilepsy-related first aid skills of family caregivers. (目的 探讨多元化健康教育在脑肿瘤相关性癫痫患者及其家属中的应用效果。方法 选取科室2019年1月—2021年12月收治的84例脑肿瘤相关性癫痫患者及其家属作为研究对象, 按入院顺序将患者分为对照组及研究组, 各42例。对照组采取常规健康教育, 研究组采取多元化健康教育。比较两组患者癫痫疾病知识掌握情况、患者服药依从性及家属急救技能掌握情况。结果 干预后, 研究组患者疾病认知得分、患者服药依从性均高于对照组, 差异有统计学意义(P

Sestrin 3 （Sesn3） is a highly conserved stress-inducible protein that is stimulated during various stress conditions, including DNA damage, oxidative stress, endoplasmic reticulum stress and inflammation, to protect organisms against different types of stimuli. Sesn3 is regulated by forkhead box O transcription factors, which activates both AMP-activated protein kinase and mammalian target of rapamycin complex 2（ mTORC2） and suppresses mTORC1. Moreover, Sesn3 can directly or indirectly scavenge reactive oxygen species to alleviate cellular oxidative damage. Sesn3 also plays an important role in the development of multiple diseases by participating in biological processes such as antioxidation, anti-aging, nutrient sensing, and maintenance of metabolic homeostasis. This study reviews the role of Sesn3 in cardiovascular and fatty liver diseases, diabetes mellitus, malignancies and epilepsy to explore novel concepts and potential therapeutic targets for addressing related diseases. [ABSTRACT FROM AUTHOR]

AIM: To study the therapeutic effect of total alkaloids of Rhizoma Corydalis（ TAC） on pentetrazol （PTZ）-induced chronic epileptic rats, and to explore its effect on nucleotide-binding oligomerization domain-like receptor protein 3 （NLRP3）/caspase-1/gasdermin D （GSDMD）-mediated pyroptosis in the cerebral cortex and its possible mechanism. METHODS: Six rats were randomly selected from 30 SPF male SD rats as the normal group, and the remaining rats were intraperitoneally injected with PTZ （35 mg/kg） for 28 d to replicate the chronic epilepsy model. The 18 successfully modeled rats were randomly divided into model, low-dose TAC （TAC-L） and high-dose TAC （TAC-H） groups, with 6 rats in each group. The rats in TAC-L and TAC-H groups received 7 and 14 mg/kg of TAC by continuous lavage for 14 d, while those in normal and model groups were given equal volume of saline. The latency of generalized tonic-clonic seizures （GTCS） and minimal clonic seizures （MCS） was recorded. The pathological changes of the temporal lobe cortex were observed by HE staining. Neuronal apoptosis was detected by TUNEL staining. The protein levels of NF-κB, NLRP3, caspase1 and GSDMD in the cortex were detected by immunohistochemical staining and Western blot. The expression levels of TNF-α, interleukin-18 （IL-18） and IL-1β in brain tissue were detected by immunohistochemical staining. The mRNA expression levels of NLRP3, caspase-1 and GSDMD in the cerebral cortex were detected by RT-qPCR. RESULTS: Compared with normal group, the latency of GTCS and MCS in the rats of model group was significantly shortened （P<0. 01）. Compared with normal group, the number of apoptotic cortical neurons in model group was significantly increased. The protein expression levels of TNF-α, IL-18 and IL-1β in the cortical tissues were significantly increased, and the mRNA and protein expression levels of NF-κB, NLRP3, caspase-1 and GSDMD were also significantly increased （P<0. 05 or P< 0. 01）. Compared with model group, the latency of GTCS and MCS in TAC treament groups was significantly prolonged, the number of apoptotic cortical neurons in rats was significantly reduced, the protein expression levels of TNF-α, IL-18 and IL-1β in cortical tissue was reduced to varying degrees, and the mRNA and protein expression of NF-κB, NLRP3, caspase-1 and GSDMD also showed varying degrees of reduction （P<0. 01）. CONCLUSION: The TAC may reduce the secretion of inflammatory factors and inhibit the pyroptosis of nerve cells through the NLRP3/caspase-1/GSDMD signaling pathway, thus playing a therapeutic role in chronic epileptic rats induced by PTZ. [ABSTRACT FROM AUTHOR]

Focal segmental glomerulosclerosis and neurodevelopmental syndrome (FSGSNEDS) is a rare autosomal dominant disease caused by the mutation of TRIM8 gene encoding tripartite motif protein 8 on chromosome 10q24.32, which is characterized by developmental delay, renal diseases and epilepsy. FSGSNEDS often progresses to end-stage renal disease after the illness sets in. The phenotype of patients varies greatly, while there are few related studies. A total of 24 cases have been described until September 15, 2023. The definite diagnosis mainly depends on gene testing. At present, there is no specific therapy for FSGSNEDS, and the treatment methods are mainly targeted in the supportive care. Patients usually presented pharmaco -resistant epilepsy, and renal abnormalities were resistant to steroid therapy, whereas optimistic prognosis was expected undergoing early renal transplantation. This article reviews the advances in FSGSNEDS, in order to further improve the understanding of this disease. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Frontiers of Computer Science & Technology is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Computer Engineering & Applications is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This paper summarized the rehabilitation nursing management of a patient with severe traumatic brain injury complicated with multiple rupture of thoracic-abdominal organs based on the concept of enhanced recovery after surgery. Based on the enhanced recovery after surgery, a multidisciplinary team for rehabilitation management was set up. Comprehensive treatment and nursing interventions including postoperative management of intracranial pressure, management of traumatic liver injuries, exercise rehabilitation, postoperative thromboembolism prevention, enteral nutrition support and management of antiepileptic drug were carried out to prevent postoperative complications and improve the rehabilitation. (本文总结1例基于加速康复外科(ERAS)理念的重型颅脑创伤伴肝脾破裂、肋骨骨折、肺、肾挫裂伤危重症患者的康复护理经验。基于ERAS理念, 组建多学科康复管理团队, 制定全面的康复治疗方案, 包括术后颅内压管理、创伤性肝损伤康复、运动康复管理、术后血栓预防管理、术后肠内营养管理、术后抗癫痫等详细的康复方案, 有效预防相关并发症, 促进患者康复。)

Epilepsy is one of the five major neuropsychiatric diseases that the World Health Organization focuses on. There are more than 10 million people with epilepsy in China, which lead to tremendous burden of disease. Fortunately, over 70% of epilepsy patients can achieve the treatment goal of seizure control and reintegration into society under standardized treatment. Improving the qualityof diagnosis and treatment of epilepsy can effectively improve the accessibility of medical care in people with epilepsy in China. In 2017, with the support of the National Health Commission, the National Center for Healthcare Quality Management in Neurological Diseases set up the Epilepsy Medical Working Group to start the construction of the medical quality control system in epilepsy and convulsive status epilepticus. The construction of medical quality control system for epilepsy and convulsive status epilepticus strengthened the national epilepsy management system, found out the status of diagnosis and treatment in China, built a dynamic feedback mechanism for the medical quality system with multiple data sources complementing each other. Applying medical quality tools for epilepsy and convulsive status epilepticus, which have continued to promote the homogenization of epilepsy diagnosis and treatment in China. [ABSTRACT FROM AUTHOR]

Objective: The current study aims to establish and evaluatee the kindling model of epilepsy induced by kainic acid (KA) microinjection into piriform cortex. Methods: Using c⁃fos immunofluorescence staining and TdT⁃mediated dUTP nick⁃end labeling (TUNEL)staining techniques to find the piriform cortex associated with epilepsy in a acute epilepsy mouse model. Racine score and seizure latency were observed after injection of KA in piriform cortex. Behavioral experiments were performed including testing the learning and memory abilities of mice in a water maze and the exploration ability of mice in a new object. The results of seizures and behavioral experiments were compared with the epileptic model of stereotactic injection of KA into the hippocampus. The electrical activities of new model mouse neurons were measured by electrophysiological techniques. Results: The results of c ⁃ fos immunofluorescence and TUNEL staining showed that in addition to the hippocampus, neurons in the piriform cortex and hippocampus were also significantly activated, and apoptosis occurred in the acute epilepsy mouse model. After injecting KA into the piriform cortex, the epilepsy was successfully ignited. Compared with the epilepsy model injected KA into the hippocampus, there was no significant difference in the epileptic seizure score and the seizurelatency was shorter. The behavioral results showed that the epileptic model mice induced by KA injection into the piriform cortex had a reduced spatial memory ability and a reduced ability to explore new objects. These results showed no significant difference compared to the epileptic model induced by KA injection into the hippocampus. The patch clamp recording test showed an increase in firing frequency and the resting membrane potential, and a decrease in amplitude of neuronal discharge. Conclusion: The microinjection of KA into the piriform cortex has successfully induced epilepsy, which may provide a new animal model for studying the pathogenesis of epilepsy. [ABSTRACT FROM AUTHOR]

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T he febrile convulsions is a common type of Convulsive disorders in children, affecting children and their parents physical and psychological health. This paper reviewed the studies about febrile convulsions in children in perspectives of clinical features, influencing factors, prognosis and fever management, anticonvulsive management and parental interventions, and provided reference for the clinical management and further studies on febrile convulsions. (高热惊厥(FC)是小儿时期最常见的惊厥性疾病, 影响患儿及家属的身心健康。本研究结合近年来国内外相关文献, 从临床特征、相关因素、预后及发热管理、抗惊厥管理、父母干预管理等方面进行综述, 旨在为FC的临床管理和后续研究提供参考依据。)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Computer Engineering & Applications is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective：To discuss the expression of circs_EFCAB2 in epileptic cell model in vitro, and to clarify its possible mechanism. Methods：The magnesium-free epilepsy cell model (model group) was constructed in the human neuroblastoma LA-N-5 cells, and the cells untreated with magnesium-free extracellular fluid were regarded as control group. Real-time fluorescence quantitative PCR (RT-qPCR) method was used to detect the expression level of circ_EFCAB2 mRNA in the cells in two groups. The cells were didived into ribonuclease (RNase) R digestion group and RNase R undigestion group. RNA enzyme digestion experiment and RT-qPCR method were used to detect the expression levels of circ_ EFCAB2 and linear EFCAB2 mRNA in the cells in two groups; nuclear cytoplasmic separation experiment was used to detect the expression levels of circle_EFCAB2 mRNA in the epileptic cells; CCK-8 method was used to detect the proliferation activities of the cells in two groups; flow cytometry was used to detect the apoptotic rate and percentages of the cells at different cell cycles in two groups. Results：The cells in model group showed spontaneous high-frequency epileptiform discharges, indicating the epilepsy cell model was successfully established. The RT-qPCR results showed that compared with control group, the expression level of circ_ EFCAB2 mRNA in the cells in model group was increased (P<0. 05). The RNA enzyme digestion experiment and RT-qPCR results showed that compared with RNase R undigestion group, the expression level of linear EFCAB2 mRNA in the cells in RNase R digestion group was decreased (P<0. 01). The nuclear cytoplasmic separation experiment results showed that there was no statistically significant difference in the expression level of circ_ EFCAB2 mRNA in the epileptic cells between the cytoplasm and nucleus of the epileptic cells (P>0. 05). The CCK-8 results showed that at 72 h after transfection, compared with control group, the proliferation activity of the cells in model group was significantly decreased (P<0. 01). The flow cytometry analysis results showed that compared with control group, the apoptotic rate of the cells in model group was significantly increased (P<0. 01); compared with control group, the percentage of the cells at S phase in model group was significantly decreased (P< 0. 01), and the percentage of the cells at G1+G2 phase was significantly increased (P< 0. 01). Conclusion：The upregulated circ_ EFCAB2 may play a role in the pathogenesis of epilepsy by inhibiting the cell proliferation, promoting the apoptosis, and blocking the cell cycles. [ABSTRACT FROM AUTHOR]

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the effects of rat nerve growth factor combined with ganglioside sodium injection on cognitive function, serum NSE and hippocampal neurons in epileptic rats. Methods: 50 male Sprague-Dawley rats were randomly divided into5 groups, including blank group, model group, A group, B group, C group. The rats in the blank group were intraperitoneally injected with the same volume of normal saline, and the rats in the other groups were intraperitoneally injected with lithium chloride and pilocarpine to establish the epilepsy rat model. Rats in blank group and model group were injected with 20 μL sterile normal saline with microsyringes. Rats in A group were intraperitoneally injected with 2000 AU/kg rat nerve growth factor at the same time point. Rats in B group were intraperitoneally injected with 20 μL ganglioside sodium at the same time point. C group was intraperitoneally injected with2000 AU/kg rat nerve growth factor combined with 20 μL ganglioside sodium. Morris water maze test and space exploration test were conducted to detect serum NSE level and hippocampal neurons, and to detect serum NSE level, neuron survival rate and nuclear factor kB P56, TLR-4 and My D88 protein levels in hippocampal nerve tissue. Results: The total swimming distance at 1,2,3,4, and 5 d were higher than that of the other four groups, higher in blank than A, B and C, B and A than C(P＜0.05), and no difference between groups B and A(P＞0.05); The number of platform crossing and exploration effective time ratio were lower than group blank, A, B and C, lower than group A, B and C, B and C(P＜0.05), and no difference between group B and A(P＞0.05); Serum NSE levels, nuclear factors kB p56,TLR-4 and My D88 protein levels were lower than blank, A, B, C, higher neuronal survival, A, B, C, lower neuronal survival, C, higher(P＜0.05), NSE, neuronal survival, and nuclear factors kB p56, TLR-4 and My D88 protein levels(P＞0.05). Conclusion: Rat nerve growth factor combined with ganglioside sodium injection can improve cognitive function and reduce hippocampal inflammatory response in epileptic rats, which may be related to the reduction of serum NSE and hippocampal nuclear factor kB P56, TLR-4, My D88 levels. [ABSTRACT FROM AUTHOR]