Search

Objective To investigate value of preoperative magnetic resonance imaging (MRI) T2 fat suppression sequence in predicting isocitrate dehydrogenase (IDH)-1 gene expression in gliomas. Methods 124 patients with glioma, who were confirmed by histopathology, were collected. Regions of interest (ROI) was delineated using ITK-SNAP software. Pyradiomics package was used to extract radiomic features from the imaging data, while a pre-trained ResNet50 deep. learning model was employed to extract deep learning features. Feature selection was performed using the Pearson correlation coefficient and the Least Absolute Shrinkage and Selection Operator (LASSO) regression model. Model performance was evaluated through 10-fold cross-validation. Traditional radiomics, deep transfer learning, and fusion models were separately constructed based on support vector machine (SVM), k-nearest neighbors (KNN), and random forest (RF) machine learning algorithms. The predictive performance of each model was assessed using receiver operating characteristic (ROC) curve. Results The area under curve (AUC) for the machine learning models SVM, KNN, and RF based on radiomic features were 0.699, 0.628, and 0.616, respectively. For the machine learning models SVM, KNN, and RF hased on deep transfer learning features, the AUC values were 0.853, 0.753, and 0.807, respectively. The machine learning models SVM, KNN, and RF based on fusion features achieved AUC values of 0. 868, 0.818, and 0. 787, respectively. Conclusion The SVM fusion model based on the T2 WI fat suppression sequence in routine MRI exhibits higher predictive performance in determining the IDH-1 gene expression status in gliomas. [ABSTRACT FROM AUTHOR]

Suspended HEK293（human embryonic kidney）cell transfection based on PEI（polyethyleneimine）is a prospective technology. However, the components of the medium often affect the transfection efficiency, which needs to be changed with transfection medium by centrifugation. This process is cumbersome and not only has the risk of contamination, but also affects the cell state. In order to fundamentally solve this problem, the influence mechanism of key components that inhibit the efficiency of transfection on the transition process need to be explored. First we explored that the key composition that have potential effects on transfection efficiency in the medium is ferric ammonium citrate（FAC）, and determined the its inhibitory effect. Then we analyzed its mechanism for inhibiting transit efficiency, including the effects of ferric ammonium citrate on the cell state, PEI-DNA complex formation, and destination gene expression. Results showed that certain amount of FAC had significant inhibitory effect on the transfection that was also gradually enhanced with concentration increasing. Further studies found that the transfection was inhibited only when citrate and iron ions were present at the same time. Over high concentration of FAC increased the particle size of the PEI-DNA complex, making it more difficult to enter the cell. This led to a reduction in the number of complexes entering the cells, and ultimately resulted in a significant drop in cell transfection efficiency. FAC restricted DNA into cells by affecting the size of the PEI-DNA complex, thereby inhibiting PEI-mediated suspended HEK293 cell transfusion process. The inhibitory effect can be eliminated when the FAC concentration was < 20 μmol/L. Through this study, the effect of FAC on the transfection process of PEI-mediated suspended HEK293 cells and its mechanism were deeply understood, providing a solid reference for the development and rational design of the transfection medium for suspended HEK293 cells. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Clinical Healthcare is the property of Chinese Journal of Clinical Healthcare and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Harbour Engineering is the property of Editorial Office of China Harbour Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bearing is the property of Bearing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forest Engineering is the property of Forest Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective： A method for determination of five sugar alcohols （erythritol, xylitol, sorbitol, maltitol and lactitol） by pres- surized capillary electrochromatography with micro flow evaporative light scattering detector was established. Methods： The chromato- graphic separation was conducted on an hydrophilic column using 80% acetonitrile-water （containing 40 mmol/L triethylamine） as the mobile phase, with＋5 kV applied voltage. The drift tube temperature of the ELSD was set at 120℃and the nitrogen flow rate was set at 0.8 L/min. Results： It was found that the reasonable linearity was achieved for five sugar alcohols with correlation coefficients no less than 0.998.The average recovery was between 82.4%-107.2% under three different level by using standard addition method, and the rela- tive standard deviation （RSD, n=3） was less than 4.0%. The limit of detection （LOD, S/N=3） was between 4 to 11 ng. Conclusions： Com- pared with the conventional HPLC-ELSD, the method has the advantages of high separation speed, good reproducibility, and environ- ment-friendly economy. The proposed method provides a fast and effective method for the quantitative detection of sugar alcohols in sug- ar-flee foods. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Based on the engineering practice of Xianrendao deep water oil terminal, we proposed a new type of structure which can be adaptable to the capacity and working conditions of a precasting yard on site. The structure has some of the partitions removed and be stiffened by using ring ribs to cut down the weight of a caisson as well as its cost. A three-dimensional FEM numerical model was used to analyze the internal force on the walls under the prevailing action of wave forces, and produce the curves of the bending moments and membrane stress distributed in different directions. The results show this type of caissons sustains small transverse bending moment, high membrane stress and the stresses act more rationally, making the structural proposal feasible and providing a new concept for the building of large deep water wharfs and terminals. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Lung Cancer is the property of Chinese Journal of Lung Cancer and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)