1. 蓝莓根际土壤微生物群落结构及促生菌筛选.
- Author
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王梦姣 and 郑天骄
- Subjects
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RHIZOBACTERIA , *INDUSTRIAL capacity , *PLANT-soil relationships , *MICROBIAL communities , *RHIZOSPHERE , *AUXIN , *BLUEBERRIES - Abstract
[Objective] The microecological structure of blueberry rhizosphere soil was analyzed from the perspective of growth-promoting mi- croorganisms to improve the blueberry planting soil. 【Method】 The microbial community structure of blueberry rhizosphere soil was analyzed by high-throughput amplicon sequencing. The growth-promoting characteristics of culturable bacteria in blueberry rhizosphere were analyzed by selective medium and colorimetry. [Result] The relative abundance of 10 bacterial phyla and 3 fungal phyla was greater than 1%, and the relative abundance of 13 bacterial genera and 11 fungal genera was greater than 1%. Occallatibacter OTU6, Paraburkholderia OTU3, and Chujaibacter OTU945 represented the highest numbers of counts more than other bacterial OTU sequences. Pseudogymnoascus OTU1, Fusar- ium OTU7, and Lentinus OTU2 represented the highest numbers of counts more than other fungal OTU sequences. The growth-promoting characteristics analysis of culturable bacteria in blueberry rhizosphere soil showed that, 12 growth-promoting bacteria that could produce aux- in were screened from culturable bacteria in blueberry rhizosphere soil, and L7 strain had the highest auxin production. These 12 growth-pro- moting bacteria could grow on the phosphorus-solubilizing medium, and had significant phosphorus decomposition halos. Strain L42 had the strongest phosphorus-solubilizing capacity. Strain L11 and L17 could break down silicates. There was no inhibition between L17 strain and L21, L26, L37, L40, L41. But there was strong inhibition between L21 and L26, L40 strains. 16S rDNA sequencing results showed that, these strains were identified as Buttiauxella, Paraburkholderia and Pseudomonas. [Conclusion] The results indicated that the diversity of mi- crobial community structure in blueberry rhizosphere was significantly different from that of other economic crops, and the growth-promoting bacteria had good phosphorus-solubilizing effect and auxin production capacity, which could provide experimental basis for the subsequent development of blueberry rhizosphere microbial fertilizer. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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