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Objective To investigate the effect of anti-cancer drug POMHEX on sperm function in mature mice. Methods Mouse epididymal sperm were exposed to POMHEX (0. 1-100 juumol/L) in vitro. Total and progressive motility of sperms were assessed using computer-as sis ted semen analysis system (CASA), and their viability was evaluated by Eosin-nigrosine staining. ATP level of sperm was detected by luciferin-luciferase system. Western blot analysis was performed to assess protein phosphorylation, and Coomassie brilliant blue G250 staining was used to estimate acrosome integrity. Results POMHEX with concentrations of 0. 1 |xmol/L significantly attenuated total and progressive motility of sperms (P < 0. 05), exhibiting a dose-dependent effect, but no effect on sperm viability. Additionally, POMHEX with concentrations of M 1 |xmol/L significantly inhibited ATP level in sperms and protein tyrosine phosphorylation during sperm capacitation in mice (Pc 0. 01). Although there was no effect on the spontaneous acrosome reaction of mouse sperm, POMHEX with concentrations of P5 pjnol/L inhibited Ca2+ ionophore A23187-induced acrosome reaction (P < 0. 01). Conclusion Our results reveal that the POMHEX can impede sperm motility and capacitation in vitro, and then potentially result in reproductive toxicity in mouse sperm. Our findings suggest that patients undergoing POMHEX treatment should consider fertility preservation options in advance. [ABSTRACT FROM AUTHOR]

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Objective To investigate the mechanism of benzodiazepine receptor antagonist ZK93426 on promoting capacitation-associated increase in protein tyrosine phosphorylation. Methods After the mouse sperm incubated with ZK93426, changes of intracellular calcium ion concentration ([Ca2+ ]i)was measured using calcium fluorescent probe Fluo4-AM; Changes of intracellular pH (pHi)was measured using pH fluorescent probe BCECF-AM; Changes of intracellular chloride ion concentration ([Cl-]i)was measured using chloride fluorescent probe MQAE; And cell membrane potential (Em) were detected using fluorescence probe DiSC3(5). Results Compared to DMSO control group,ZK93426 promoted the capacitation-associated increase in protein tyrosine phosphorylation in mice, accompanied by the increase of [Ca2+ ]i and pHi and the decrease of [Cl-]i. Membrane hyperpolarization was inhibited by ZK93426 as well. Conclusion ZK93426 may regulate the capacitation-associated increase in tyrosine phosphorylation of mouse sperm by modulating [Ca2+ ]i, [Cl-]i, pHi and Em. [ABSTRACT FROM AUTHOR]

he perfluorohexyl anionic and gemini fluorine-containing surfactants are synthesized by one step or two steps reaction of perfluorohexyl sulfonyl chlorid, the surface activity and high yield were reported, and the effects of salt and acid-base on the surface activity of the synthesized surfactants were studied. The results showed that both surfactants exhibited excellent surface property and resistance to salt. Nevertheless, the critical micelle concentrations of gemini fluorocarbon surfactant was as low as 6.67×10-4 mol/L, and this surfactant boasted better acid and alkali resistance. Moreover, the two fluorocarbon surfactants remained stable above 200 ℃.These superior properties indicate that these surfactants had good prospect for commercial applications. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Andrology is the property of Chinese Journal of Andrology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)