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The basement membrane is a specialized extracellular matrix between the epithelium and the mesenchyme. In stratified epithelium, only the basal cells in contact with the basement membrane exhibit the apical-basal polarity, whereas the epithelial cells do being not in contact with the basement membrane do not exhibit the apical-basal polarity. The basement membrane plays an important role in epithelial cell polarization. It is an important extracellular matrix (ECM) structure in the multicellular organisms, is situated between the epithelium and the mesenchyme, and is produced jointly by the epithelial cells and mesenchymal cells. Its components mainly include Laminin, type Ⅳ collagen (Col-Ⅳ), nidogen(NDG), and heparan sulfate proteoglycans (HSPG), and each component plays the different role in influencing the epithelial cell polarity. The network scaffold formed by Col-Ⅳ and Laminin is the main structure of the basement membrane, and the integrity of the structure affects the epithelial cell polarization. This review summarizes the composition and structure of the basement membrane, focuses on its role in epithelial cell polarization and its mechanism, and compiles the current status of biomimetic basement membrane materials that promotes the epithelial cell polarization, and provides the theoretical foundation for further exploration of the establishment and maintenance of epithelial cell polarity. [ABSTRACT FROM AUTHOR]

Objective·To study the effect of phosphatase and tensin homolog on chromosome 10 (PTEN) on alternative splicing of forkhead box M1 (FoxM1), and its impact on tumor cell migration. Methods·PTEN was knocked down by short hairpin RNA (shRNA) in human embryonic kidney 293T cells, human prostate cancer DU145 cells, human colorectal adenocarcinoma RKO cells, and human colon cancer SW480 and SW620 cells. Specific primers were designed for FoxM1 and its subtypes FoxM1B and FoxM1C, and the mRNA expression levels of FoxM1B and FoxM1C were detected by quantitative real-time PCR (qRT-PCR). FoxM1B and FoxM1C were overexpressed in DU145 cells, and their effects on tumor cell migration were tested by Transwell assay and wound healing assay. Immunofluorescence and dual luciferase reporter gene assay were used to explore the potential mechanism of differential regulation of tumor cell migration by FoxM1B and FoxM1C. Results·① PTEN was knocked down in 293T, DU145, RKO, SW480, and SW620 cell lines. qRT-PCR results showed that compared with the control cells, the mRNA expression level of FoxM1B significantly increased in PTEN knockdown cells, while the mRNA expression level of FoxM1C decreased or remained unchanged. Knockdown of PTEN did not affect the transcription level of FoxM1, but caused the variable splicing of FoxM1 and promoted the generation of FoxM1B. ② Compared with the control cells, the number of DU145 cells migrating to the below chamber increased in the FoxM1B overexpression group (P=0.024), while the number of migrating DU145 cells in the FoxM1C overexpression group was lower (P=0.000). The healing ability of DU145 cells was significantly enhanced in the FoxM1B overexpression group (P=0.001), while the healing ability of DU145 cells was weakened in the FoxM1C overexpression group (P= 0.021). Overall, FoxM1B and FoxM1C had opposite effects on tumor cell migration. FoxM1B promoted tumor cell migration, while FoxM1C inhibited tumor cell migration. ③ Neither FoxM1B nor FoxM1C overexpression could induce β -catenin to enter the nucleus. Dual luciferase reporter gene assay showed no difference in the transcriptional activity of FoxM1B and FoxM1C. The difference between FoxM1B and FoxM1C in the regulation of tumor metastasis was also not mediated by β-catenin translocation. Conclusion·Knockdown of PTEN regulates the alternative splicing of FoxM1, leading to increasing expression of transcript FoxM1B, which plays a positive role in tumor cell migration. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the efficacy of compound Zaofan pills combined with recombinant human granulocyte colony-stimulating factor (rhG-CSF) in the treatment of leukopenia after tumor chemotherapy. METHODS: A total of 94 patients with leukopenia after tumor chemotherapy admitted into the hospital in 2021 were extracted to be divided into the control group and the observation group via the random number table method, with 47 cases in each group. The control group received rhG-CSF treatment, while the observation group was given compound Zaofan pills combined with rhG-CSF treatment. The clinical efficacy, traditional Chinese medicine syndrome score, incidence of adverse drug reactions, peripheral blood white blood cell (WBC) count, neutrophil count (NEUT), CD3 +, CD4 +, CD8 + and CD4 + / CD8 + were compared between two groups. RESULTS: The total effective rate of the observation group was 89. 36% (42 / 47), significantly higher than 72. 34% (34 / 47) of the control group, the difference was statistically significant (P<0. 05). After treatment, the traditional Chinese medicine syndrome score and peripheral blood CD8 + levels decreased significantly, while peripheral blood WBC, NEUT, CD3 + , CD4 + and CD4 + / CD8 + levels increased significantly in two groups; the traditional Chinese medicine syndrome score and peripheral blood CD8 + levels of the observation group were significantly lower than those of the control group, and the peripheral blood WBC, NEUT, CD3 +, CD4 + and CD4 + / CD8 + levels of the observation group were significantly higher than those of the control group, the differences were statistically significant (P<0. 05). The incidence of adverse drug reactions in the control group and the observation group were respectively 17. 02% ( 8 / 47) and 12. 77% ( 6 / 47), and there was no significant difference between two groups (P>0. 05). CONCLUSIONS: The efficacy of Zaofan pills combined with rhG-CSF in the treatment of leukopenia after tumor chemotherapy is significant, which can effectively increase the WBC and improve the immune function with higher safety. [ABSTRACT FROM AUTHOR]

Objective To analyze the errors occurring in the pharmacy intravenous admixture service（PIVAS） and propose preventive measures to ensure the safety of patients’ medication.Methods The number and types of errors in PIVAS of this hospital in 2021 were summarized and analyzed by Excel 2007 software and the corresponding preventive measures were put forward.Results In 2021,a total of 14 303 300 infusions were configured in PIVAS of this hospital, and 1 148 errors occurred, with an error rate of 0.80/10 000.Drug discharge errors accounted for 53.05%（609/1 148） and configuration errors accounted for 30.40%（349/1 148）.Conclusion Preventive measures such as improving the system, strengthening training, and updating equipment can reduce errors and improve the quality of pharmacy services. [ABSTRACT FROM AUTHOR]

As one of the important contents of deepening the reform of education evaluation, teacher training effectiveness evaluation currently has the following problems: it is not diagnostic due to the lack of integrated and through design; it stops at the superficial point because of simply emphasizing “students satisfaction' it becomes a mere formality owing to the low reliability and validity of the assessment questionnaire; it is impossible to touch the real points due to the non-individualization and non-customization of the rubrics; there are structural defects owing to emphasizing teaching evaluation and neglecting learning evaluation. Based on the research results of domestic and foreign scholars on teacher training effectiveness evaluation and the results of the author爷s investigation, this paper puts forward some suggestions on the construction of teacher training effectiveness evaluation system: to find out the logical starting point of teacher training evaluation on the basis of the demand of the “four-in-one冶model and the explicit goal transformation of behavior, to integrate pre-evaluation, real-time evaluation and time-delayed evaluation to ensure the coverage of the entire process of teacher training effectiveness evaluation, to construct the interactive system of teacher training evaluation with diversified evaluation orientations, diversified evaluation methods, and specialized evaluation tools, to control teacher training evaluation rubrics with layered, classified, and sub-disciplined customized observation points, and to construct teacher training evaluation index system by adopting the “five-fold transformation-model of academic evaluation. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To design the scale for children’ s compliance and attitude towards traditional Chinese medicine decoction, so as to measure the reliability and validity of the scale. METHODS: The above scale was used to evaluate the compliance and attitude towards traditional Chinese medicine decoction in children in Traditional Chinese Medicine Department of Beijing Children’s Hospital. Item analysis, homogeneity, reliability and validity of the scale were carried out. RESULTS: Eighty-Five questionnaires were collected, of which 80 were valid questionnaires, and the questionnaire response rate was 94. 1%. Item analysis showed that, except for item 5 of compliance, the difference of t-test for variance of other items was statistically significant(P<0. 001), with good discrimination. The homogeneity test showed that the score and total score of each item were significantly different(P<0. 001), and the homogeneity was high. The reliability analysis showed that the internal consistency coefficient(Cronbach’ s α) was >0. 697, and the adjusted Cronbach’ s α was > 0. 723, indicating the high reliability of the scale. Content validity analysis results showed that S-CVI was >0. 80, S-CVI/ Ave was >0. 90, and all experts had higher degree of recognition for the topic. The KMO value of structural validity was 0. 595, and the Bartlett’ s spherical test value was 802. 599 (P<0. 001). Two common factors were extracted by principal component analysis, which could explain 47. 490% of the total variance. The load of each item on its principal factors was >0. 400, indicating the high load value. CONCLUSIONS: The scale has good reliability and validity in measuring children’s compliance and attitude towards traditional Chinese medicine decoction, and can be popularized. [ABSTRACT FROM AUTHOR]

Objective To analyze the potential targets of self-designed Zhongfeng 1 prescription in treatment of cere⁃ bral hemorrhage，and to analyze their biological functions. Methods Firstly，the chemical components and targets of the self-designed Zhongfeng 1 prescription were collected from TCMSP，TCMID and Chemistry Database. The targets of in⁃ tracerebral hemorrhage were obtained from OMIM，DisgenET and GeneCards database. Using R software，the self-de⁃ signed Zhongfeng 1 was interacted with cerebral hemorrhage-related targets to obtain the common targets（potential thera⁃ peutic target). The obtained common targets were screened by protein-protein interaction（PPI）on the STRING platform to identify the key targets for the treatment of cerebral hemorrhage. Metascape database and R software were used to ana⁃ lyze the biological functions（GO and KEGG enrichment analysis）of the key targets of the prescription in the treatment of cerebral hemorrhage. Finally，PyMoL and Autodock Vina were used to verify the core chemical components of the prescription with the key targets for molecular docking. Results A total of 53 effective TCM chemical components, and 124 targets for drug action and 1 896 intracerebral hemorrhage-related targets were collected, and 71 potential therapeutic targets of the self-designed Zhongfeng 1 prescription were finally obtained. The key targets screened by PPI included JUN, TP53, AKT1, VEGFA, etc. A total of 1 715 GO items were enriched，and they were mainly involved in the oxidative stress, apoptotic signaling pathway, etc. Totally 130 items were enriched from KEGG pathway，mainly including PI3K-Akt signal⁃ ing pathway，P53 signaling pathway, cAMP signaling pathway，NF-κB signaling pathway，etc. The molecular docking re⁃ sults showed that the core chemical components had strong binding ability to the target. Conclusion The core targets of self-designed Zhongfeng 1 prescription in the treatment of cerebral hemorrhage include Jun, TP53, Akt1, and VEGFA, etc，they inhibit the inflammatory response and apoptosis，reduce oxidative stress；the related signal pathways mainly include PI3K-AKT，P53, cAMP, NF-κB，and other signal pathways. [ABSTRACT FROM AUTHOR]

Objective: To explore the relationship between the levels of serum uric acid (UA), cystatin C (CysC) and lipoprotein-associated phospholipase A2 (Lp-PLA2) and prognosis of patients with acute cerebral infarction complicated with leukoaraiosis. Methods: 113 patients with acute cerebral infarction complicated with leukoaraiosis admitted to the 960 Hospital of Joint Logistics Support Force of Chinese People's Liberation Army from March 2020 to December 2022 were selected, the levels of serum UA, CysC and Lp-PLA2 were detected. Followed up for 1 month, the patients were divided into good prognosis group (0 ~ 2 scores, 75 cases) and poor prognosis group (3 scores and above, 38 cases) according to the modified Rankin Scale (mRS) score. Multivariate Logistic regression was used to analyze the risk factors of poor prognosis in patients with acute cerebral infarction complicated with leukoaraiosis, and receiver operating characteristic curve (ROC) was used to analyze the value of serum UA, CysC and Lp-PLA2 in predicting poor prognosis in patients with acute cerebral infarction complicated with leukoaraiosis. Results: The levels of serum UA, CysC, and Lp-PLA2 in the poor prognosis group were higher than those in the good prognosis group (P<0.05). Multivariate Logistic regression analysis showed that severe white matter lesions, high NIHSS score at admission, and high levels of serum UA, CysC, and Lp-PLA2 were risk factors for poor prognosis in patients with acute cerebral infarction complicated with leukoaraiosis (P<0.05). The area under the curve (AUC) of combined serum UA, CysC, and Lp-PLA2 to predict the prognosis of patients with acute cerebral infarction complicated with leukoaraiosis was 0.916, which was higher than the individual prediction. Conclusion: The elevated levels of serum UA, CysC, and Lp-PLA2 in patients with acute cerebral infarction complicated with leukoaraiosis were associated with poor prognosis, the combination of serum UA, CysC, and Lp-PLA2 had a higher predictive value for poor prognosis in patients with acute cerebral infarction complicated with leukoaraiosis. [ABSTRACT FROM AUTHOR]

[Objective] The present paper was conducted to popularize the natural herbage among vineyards in the eastern foothill of Helan Mountain. [Method] In this experiment, natural herbage for 1 year and cleared vineyard were taken as contrast. The effects of natural herbage on soil nutrients, enzyme activity and microorganism in different soil layers of vineyard were studied in Yunhao winery of Lanshan, Jins-han, eastern foothill of Helan Mountain. [Result] The soil alkali-hydrolyzed nitrogen and available phosphorus content were higher than that of the control, however, the pH, total salt, total nitrogen, total phosphorus, available potassium and organic matter content decreased, the number of bacteria, fungi and actinomycetes in different soil layers of natural herbage for 1 year vineyard soil increased,and the deep soil layer (40 -60 cm) was more obvious than the surface soil layer (0-20 cm), and natural herbage for 1 year could improve urease, sucrase and catalase in vineyard, especially for the surface soil layer (0 -20 cm), and the increase of soil enzyme activity was more obvious. [Conclusion]The natural herbage in vineyard can improve the soil condition to some extent, reducing soil pH, decreasing soil salt content, increasing soil microorganism and improving soil enzyme activity that provides a comfortable growth environment for grape growth, and then improves grape and wine quality, which is a good management method for vineyard soil in the eastern foothill of Helan mountain. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of 1-year non-stop alendronate treatment on the quality of life of postmenopausal osteoporotic patients. Methods A total of 265 patients with postmenopausal osteoporosis who were admitted to our hospital from May 2015 to December 2016 and received alendronate treatment of 70 mg per week were selected. During the 1-year observation period from the start of selection, the quality of life at baseline and 3, 6, and 12 months after enrollment was evaluated using European QOL 5 dimensional (EQ-5D) and Japan Osteoporosis Quality of Life Questionnaire (JOQOL). Results In 265 evaluable patients, a significant improvement in quality of life was observed from 3 months after enrollment and remained constant throughout the 1-year observation period. After treatment both EQ-5D and JOQOL were significantly improved (P<0． 05) within 1 year of treatment. Factors identified as causing changes in QOL included: fractures within one year before enrollment, skeletal deformities, osteoarthritis, use of active vitamin D3, age based on JOQOL, and vertebral deformities. The result showed that the patient's perception of the therapeutic effect, such as pain improvement, contributed to the continuation of the treatment. Patients with osteoporosis knew that once a week of bisphosphonate treatment for sustainable treatment, regardless of the complications of exercise disorders, could significantly improve the quality of life and encouraged them to continue treatment. Conclusion Continuous bisphosphonates treatment improves quality of life even in patients with motor diseases. The use of active vitamin D3 also helps to further improve the quality of life. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Shenkang injection on the renal and cardiac function of hypertensive nephropathy patients complicated with hyperhomocysteinemia. Metiiods: A total of 76 patients with hypertensive nephropathy complicated with hyperhomocysteinemia were divided into the study group and the control group with 38 cases in each group. Both groups were given routine treatment, and the study group was additionally treated with Shenkang injection for 4 weeks. The renal function index [serum creatinine (SCr), blood urea nitrogen (BUN), endogenous creatinine clearance rate (Ccr)], renal injury markers [serum p2- microglobulin (p2-MG), serum cystatin C (Cys-C)], cardiac function index [left ventricular ejection fraction (LVEF), left ventricular end diastolic diameter (LVIDd), left ventricular end systolic diameter (LVIDs)], inflammatory factors [C reactive protein (CRP), tumor necrosis factor a (TNF- a), interleukin -6 (IL-6)] and risk factors of atherosclerosis [homocysteine (Hcy), endothelin (ET)] and the incidence of adverse reactions were compared between the two groups. Results: At 4 weeks after treatment, the levels of renal function indexes (SCr, BUN), renal injury markers, some cardiac function indexes (LVIDd, LVIDs), inflammatory factors and risk factors of atherosclerosis were significantly lower than those before treatment, and those in the study group were lower than the control group (P<0.05). The LVEF and Ccr levels were significantly higher than those before treatment, and those in study group were higher than the control group(P<0.05). There was no adverse reactions in the two groups (P>0.05). Conclusion: Shenkang injection can delay the process of renal injury, improving cardiac and renal functions and reducing the risk of atherosclerosis in the treatment of hypertensive nephropathy patients complicated with hyperhomocysteinemia. [ABSTRACT FROM AUTHOR]

The concepts of induced (λ, μ)-fuzzy bi-ideal and (λ, μ)-fuzzy inner ideal were introduced based on the concept of the (λ, μ)-fuzzy subsemigroup. Some properties of them were also discussed. [ABSTRACT FROM AUTHOR]

Objective: To construct the lentiviral vector containing HTRA1 as well as its mutant gene(HTRA1-Mut,1091TC) and express them in HBVSMCs. Methods: HTRA1 and HTRA1-Mut gene regional fragment were amplified by RT-PCR and then cloned into the plasmid GV287.Lentiviral plasmid, p Helperl.0, and p Helper2.0 were co-transfected into 293 T cells to obtain recombinant virus. The lentiviral titer was detected. Collected lentivirus was applied to infect human vascular smooth muscle cells. Results: The recombinant lentiviral vectors were constructed and were confirmed by PCR and sequencing analysis. They could efficiently transfect 293 T cells and express in the cells. The lentiviral titer was 2E+8 TU/ml. HBVSMCs produced fluorescent expression after lentivirus transfection, and HTRA1 gene expressed over 80%. Transfected HBVSMCs grew in order without inducing excessive death rate. Conclusion: HTRA1 and HTRA1-Mut recombined lentiviruses with high viral titer were successfully constructed and packaged, and the HTRA1 and HTRA1-Mut gene could be transfected into HBVSMCs with stable and high expression in infected cells, and these cells might be applied for mechanism researches of HTRA1 gene. [ABSTRACT FROM AUTHOR]

Dual-channel frequency modulation stereo broadcast system is a typical application of analog modulation technique, FM, double side band with suppressed carrier(DSB-SC), frequency division multiplexing(FDM) and phase-locked loop(PLL) are core techniques. Based on MATLAB/Simulink platform, the auther successful designed a dual-channel frequency modulation(FM) stereo broadcast system.After debugging and testing, the system can work well,system analysis indicates that FDM technique can improve channel utilization, and PLL technique can obtain sub-carrier to demodulate DSB-SC signal.It is worth to reference for related research and performance improvement, and also provide a new exploration to experiment teaching. [ABSTRACT FROM AUTHOR]

Objective To investigate effects of tripterygium glycosides (TG) on the apoptosis of human renal tubular epithelial cells HK-2 induced by high glucose and the CXC chemokine ligand 10 (CXCL10)/CXC chemokine receptor 3 (CXCR3) axis. Methods Human renal tubular epithelial cells HK-2 were cultured in vitro and divided into the control group (culture medium containing 5.5 mmol/L glucose), the high glucose group (culture medium containing 25 mmol/L glucose) and the 12.5, 25 and 50 mg/L TG groups (culture medium containing 12.5, 25 and 50 mg/L TG and 25 mmol/L glucose, respectively). Methyl thiazolyl tetrazolium (MTT) method was used to detect the viability of HK-2 cells. Flow cytometry was used to detect the apoptosis of HK-2 cells. 2',7'-dichlorodi-hydrofluorescein diacetate method was used to detect reactive oxygen species (ROS) level of HK-2 cells. Xanthine oxidase method was used to detect superoxide dismutase (SOD) level of HK-2 cells. Enzyme-linked immunosorbent assay was used to detect levels of inflammatory factors of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in HK-2 cells. Western blot assay was used to detect the expression of apoptotic proteins (Caspase-3, Caspase-9), CXCL10 and CXCR3 proteins in HK-2 cells. Results Compared with the control group, the HK-2 cell viability and SOD level were significantly reduced in the high glucose group, and the apoptosis rate, ROS, TNF- α, TGF- β1 levels and Caspase-3, Caspase-9, CXCL10, CXCR3 protein expression levels were significantly increased (P＜0.05). Compared with the high glucose group, the HK-2 cell viability and SOD level were significantly increased in the 12.5, 25 and 50 mg/L TG groups, and the apoptosis rate, ROS, TNF-α, TGF-β 1 levels and Caspase-3, Caspase-9, CXCL10, CXCR3 protein expression levels were significantly reduced, and high dose TG has better effect (P＜0.05). Conclusion TG can inhibit high glucose induced apoptosis, oxidative stress and inflammation of HK-2 cells, and its mechanism may be related to the inhibition of CXCL10/CXCR3 axis. [ABSTRACT FROM AUTHOR]

目的 观察 2014 年度和2015年度武汉地区急性呼吸道感染（ARTIs）患儿鼻咽分泌物中呼吸道合胞病毒（RSV）、人偏肺病毒（hMPV）基因检出情况，并分析 RSV、hMPV 感染的分布特点。方法2014年1月～2015年12月收治的0～14岁的 ARTIs 患儿816例（2014年度456例、2015年度360例），采集患儿鼻咽分泌物，采用 RT-PCR 检测 RSV G 基因、hMPV F 基因，通过 DNA 测序分析基因型。观察 RSV、hMPV 感染在不同年度、季节及不同性别、年龄、病种患儿中的分布情况。结果816例患儿中，检出 RSV 187例，其中2014年度109例、2015年度78例；检出 hMPV 116例，其中2014年度69例、2015年度47例；2014年度和2015年度 hMPV、RSV 的检出率差异均无统计学意义。1～2岁年龄组患儿 hMPV 检出率最高，5岁以内患儿 hMPV 检出率高于90％；5岁以内患儿 RSV 检出率也较高，高于90％。喘息性支气管炎、毛细支气管炎患儿 hMPV 检出率显著高于其他感染部位（P 均＜0．05），上呼吸道感染、支气管炎、支气管肺炎患儿 hMPV 检出率较低（10％左右）；支气管肺炎、毛细支气管炎患儿 RSV 检出率高于其他感染部位（P 均＜0．05），喘息性支气管炎患儿 RSV 检出率最低。每年2～3月 hMPV、RSV 检出率最高，6～9月最低。基因型分析显示，2014、2015年度患儿检出 RSV 基因型均以 A 型为主，2014年度患儿检出 hMPV基因型以 A2型为主、2015年度以 B 型为主。结论 RSV 和 hMPV 是武汉地区 ARTIs 患儿常见的病原体，RSV、hMPV 感染多发生在5岁以下患儿，且多发于冬季和早春；RSV 感染与支气管肺炎、毛细支气管炎有关，hMPV 感染与喘息性支气管炎、毛细支气管炎有关；RSV 流行毒株以 A 型为主，hMPV 流行毒株在2014、2015年度存在差异. [ABSTRACT FROM AUTHOR]

Surfactant solution foam drainage is commonly used in the process of natural gas production, so the foaming effect is vital for the exploitation of natural gas. As an example, the foaming process of sodium α-olefin sulfonate was discussed at room temperature with the parameters of foam concentration and flow rate by using a drainage simulator. Then a bubble dynamics equation was established. Using single factor research method, the kinetic equation parameters were determined one by one, finally the blistering kinetic equation was confirmed. This work provides a feasible method for the research of surfactant foaming process. [ABSTRACT FROM AUTHOR]

Objective·To explore changes in myocardial mitochondrial proteinogram in spontaneously hypertensive rats (SHR) with left ventricular hypertrophy (LVH) and to investigate the effects of losartan potassium anti-hypertensive treatment on the proteinogram. Methods·20-week-old SHRs (SHR group) were randomly assigned to the 8-week losartan potassium anti-hypertensive treatment group (therapy group, 28-week-old) and the untreatment group (control group, 28-week-old). Wistar-Kyoto (WKY) rats aged 20 weeks were used as the controls of the SHR group (WKY group). Left ventricular mitochondrial proteinogram was measured using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined with MALDI-TOF/TOF tandem mass spectrometry. Blood pressure and myocardial diameter of rats were also measured. Results·Thirteen mitochondrial protein spots showed alterations by more than 1.3 folds in the SHR group compared with the WKY group. Some spots were identified as the same protein by digestion and mass spectrometry analysis. Five identified proteins were all related to the energy metabolism of mitochondria. No additional different spots were identified after 8 weeks of anti-hypertensive treatment. The therapy group had significantly lower systolic blood pressure and diastolic blood pressure than the control group (P=0.000, P=0.002) and remarkably shorter myocardial diameter in enlarged left ventricles (P=0.000). Conclusion·SHRs with LVH have mitochondrial energy metabolism dysfunction. Losartan potassium can significantly decrease the blood pressure and partially reverse LVH, but has no significant effect on mitochondrial energy metabolism proteins, suggesting changes in mitochondrial proteins are possibly not caused by the load after hypertension. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the characteristics of adverse drug reactions induced by montelukast sodium in children, so as to provide guidance for clinical rational drug use. METHODS: CNKI, Wanfang Data and PubMed were searched from the establishment of the database to Oct. 2021 to collect the reports on adverse drug reactions induced by montelukast in children. The incidence and characteristics of adverse drug reactions induced by montelukast were studied by bibliometric analysis. RESULTS: A total of 12 748 literature were initially screened in the database. After reading literature abstracts, extracting case reports, deleting adult data, and excluding uncertain disease factors, 23 literature (30 children) were included in the adverse drug reactions case reports of children. Among them, there were 13 literature (20 children) on neuropsychiatric adverse drug reactions, including nightmares, sleepwalking, excitement, emotional behavior disorders, aggression and convulsions. Digestive system adverse drug reactions were reported in 3 literature (3 children), mainly manifested as liver function injury. Urinary system adverse drug reactions were reported in 3 literature (3 children), mainly manifested as enuresis. Skin system adverse reactions were reported in 1 literature (1 child), mainly manifested as pemphigus. Auditory system adverse drug reactions were reported in 1 literature (1 child), mainly manifested as earache. Visual system adverse drug reactions were reported in 1 literature (1 child), mainly manifested as headache. Musculoskeletal system adverse drug reactions were reported in 1 literature (1 child), mainly manifested as arthralgia and muscle rigidity. CONCLUSIONS: Adverse drug reactions of montelukast in children mainly focuses on neuropsychiatric adverse drug reactions (>66%), including nightmares, anxiety, aggression and emotional behavior disorders. The symptoms disappear within a short time after the drug withdrawal. The most important thing to be alerted to is suicide. In view of the above literature analysis, if neuropsychiatric adverse drug reactions occur during clinical use of montelukast, drug-related effects should be considered and treated accordingly. [ABSTRACT FROM AUTHOR]

Pre-harvest sprouting, easily influenced by the external environment, is one of the serious disasters in wheat production, and once the occurrence not only affects the yield, but also seriously affects the quality of wheat. Therefore, it is very important to develop wheat varieties with resistance to sprouting. For a more comprehensive evaluation of wheat sprouting resistance, the seed germination, whole spike germination and field intact spike germination, the basic methods to evaluate the germination of wheat, were compared and analyzed in the research by germinating test with 65 varieties and breeder's lines. The results of correlation and difference analysis showed that the germination rates obtained in the three methods were significantly positive correlated, and significantly different at the 1% level. The correlation between germination index and seed germination rate was the highest, which could better evaluate the dormancy characteristics of wheat, but could not get the overall resistance. The coefficients of variation of the seed germination and the whole spike germination were smaller, and the test conditions were easier to control, compared with the field intact spike, so they were the simple methods to evaluate the resistance of wheat spike sprouting; The average seed germination rates of most materials were the highest and the field intact spike germination rate was the smallest for the most of accessions, and the differences among the three methods reached extremely significant levels, which showed that the external environment and the external structure of wheat on spike germination was significantly affected, so it was very important to strictly control the external environment during test. Therefore, the seed germination method could be used to screen germplasm first from the dormancy, to seek resistance materials from the source, and the whole spike germination method could be used to further verify of the sprouting resistance, to evaluate the comprehensive resistance of the pre-harvest sprouting. Meanwhile, the field intact spike germination was more susceptible to natural conditions, and its variation degree was larger. Therefore, the results could be used as reference data for laboratory germination tests and it is necessary to combine the three methods in order to obtain a more accurate result. [ABSTRACT FROM AUTHOR]

Vacuum experiment is an important experiment. But facing on the heavy task of experimental teaching and stressful teaching hours, the traditional vacuum equipment cannot meet the experiment teaching work because of various reasons. In the condition of the limited funds and the vacuum equipments that cannot scrapped, the equipments should carry out targeted transformation due to the actual needs of experimental teaching. The diffusion pump of DM-240 uses a cooling water system, but in the end of the experiment process, it needs manually determine whether to cool, then the equipment and the water system can be shutdown. Because the process needs long time, it affects the quality of experimental teaching. Therefore the diffusion pump cooling control system should be transformed into temperature automation control system. Through to monitor the temperature of diffusion pump, it can automatically shut off the cooling water system and unit equipment. It not only ensures the experimental teaching hours, but also avoids the damage of the machine due to mishandling. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate drug application in Beijing Children's Hospital Affiliated to Capital Medical University (hereinafter referred to as "our hospital"), so as to provide references for delicacy drug management. METHODS: Activity based classification (ABC) analysis, vital-essential-nonessential (VEN) analysis and ABC-VEN matrix analysis methods were adopted to analyze the varieties and consumption sum of drugs used by outpatient and inpatient in our hospital from Sept. 2018 to Aug. 2019, inventory turnover analysis and dosage analysis were conducted at the same time. RESULTS: Totally 698 kinds of drugs were used in the outpatient and inpatient in our hospital from Sept. 2018 to Aug. 2019, with the consumption sum of 669 322 100 yuan. Based on ABC analysis, the constitute ratio of varieties of drugs in categories A, B and C were respectively 15. 90% (111 kinds), 20. 20% (141 kinds) and 63. 90% (446 kinds), the constitute ratio of consumption sum were respectively 79. 85% (534 441 300 yuan), 15. 10% (101 081 400 yuan) and 5. 05% (33 799 400 yuan). Based on VEN analysis, the constitute ratio of varieties of drugs in categories V, E and N were respectively 35. 10% (245 kinds), 54. 44% (380 kinds) and 10. 46% (73 kinds), the constitute ratio of consumption sum were respectively 29. 30% (196 112 200 yuan), 63. 82% (427 185 900 yuan) and 6. 88% (46 024 000 yuan). Combined with ABC-VEN matrix analysis, the highest constituent ratio of varieties was 31. 52% (220 kinds) for drugs in CE category, the highest constituent ratio of consumption sum was 51. 76% (346 472 000 yuan) for drugs in AE category, the lowest constituent ratio of varieties was 0. 86% (6 kinds) for drugs in AN category, and the lowest constituent ratio of consumption sum was 0. 66% (4 411 800 yuan) for drugs in CN category. CONCLUSIONS: The upper and lower limits of inventory of partial CV-category and CE-category drugs should be appropriately adjusted to ensure the supply for clinical necessary and reduce the occurrence of drug shortage; there are some key drugs under national control among AE-category and AN-category drugs, reducing their use can not only reduce drug costs, but also help promoting clinical rational medication. Combining ABC analysis with VEN analysis to analyze the drug application in hospitals over a period of time is a scientific method applied to practical work, which can provide data support for the detailed management of drug inventory and clinical rational medication. [ABSTRACT FROM AUTHOR]

Objective: To study the curative efficacy of Ergosine plus misoprostol in the treatment of Placenta previa and its effects on the serum Reactive oxygen species(ROS), superoxide dismutase(SOD), glutathione peroxidase(gsh-px) levels. Methods: 100 cases of placenta previa patients admitted to our hospital from May 2016 to May 2017 were selected and divided into the observation group(n=52) and the control group(n=48) according to the random grouping method. The control group was treated with misoprostol,while the observation group was treated with ergoxin combined with misoprostol. The clinical efficacy, changes of serum ROS, SOD and GSH-px levels before and after treatment, intraoperative and postoperative blood loss, hemostasis time and incidence of adverse reactions were compared between the two groups. Results: After treatment, the total effective rates of observation group and control group were96.15% and 81.25%, respectively, which was significantly higher in the observation group than that of the control group(P<0.05). The levels of serum ROS, SOD and GSH-px in the two groups were significantly improved compared with those before treatment, and the levels of serum ROS in the observation group were lower than those in the control group, the levels of serum SOD and gsh-px were significantly higher than those in the control group(P<0.05). The intraoperative and postoperative blood loss and hemostasis time in the observation group were significantly lower than those in the control group(P<0.05). The total incidence of adverse reactions in the two groups was 7.69% and 22.92%, respectively, which was significantly lower in the observation group than that of the control group(P>0.05).Conclusion: The efficacy and safety of ergoxin combined with misoprostol in the treatment of placenta previa are significantly better than those of misoprostol alone, which may be related to the effective improvement of serum ROS, SOD and GSH-px levels. [ABSTRACT FROM AUTHOR]

目的探定氧化特款骨外救配合半导体激光身 1-3 个月給嬰儿紅骨的治疗作用方法选取 2019 年 6 月至 2020 年1月于那州大学附屬儿业區院小嬰儿科发生1~3个月齡婴儿红骨的患儿 60例作为研究对象,并非随机分为現察組和对 恩组,各30例規察组进行氧化针款骨外教配合キ导体激光治有,时照组进行氧化特软开治疗,上校两組惠儿的一般情況、治疗 此果,红帮消建时间以及护理质量,结果 两姐息儿月齡、身长、休重及治疗前的紅臀情况比教,基异均无统计学意义 (P> 0.05) 现察组总有效率为 90.00%(27/30)-対照組为 66.67%(20/30) 規容组明显高于对照组,差异有统计学意义 (P<0.05) 現 机惠儿红骨清进时间明里低子时照组,发并有候计学意义(P<0.05):現容组的护理质量明其高对照姐,差并有統计学念义 (P<0.05),结论 氧化伴教骨外教配合年导体激光治疗 1-3 个月給墨儿紅帶疗效显著可明能前超单软使用氣化伴款重时惠 儿的红學消進时间,临床治疗效果更好. [ABSTRACT FROM AUTHOR]