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【Objective】The composition of the fungal community in the root and root areas of Paphiopedilum hirsutissimum was analyzed at molecular level to provide reference for conservation and population restoration.【Method】18 S rDNA sequences from the root and rhizospheric soil of P. hirsutissimum were amplified and analyzed by the high-throughput sequencing technology for study the fungal communities in root and rhizospheric soil as well as the correlation between fungal communities in root and rhizospheric soil environment.【Result】The results showed that OUTs numbers of fungus among samples and rhizospheric soil samples were different, but the total OTUs numbers were not significantly different between root and rhizospheric soil. Root and rhizospheric soil had the similar fungal coinmuities and the fugnal communities varied with individual plants. Alpha diversity analysis showed that the fugnal community diversity had a little difference in root and rhizospheric soil, the differentiation of fungal community was low. Nectriaceae and Cordycipitaceae were the dominant fungal community both in root and rhizospheric soil. Meanwhile, UPGMA cluster analysis showed that all root samples and rhizosphere soil samples did not cluster in separate branches and indicated small differences in species diversity and abundance. In terms of dominant fungal community, Fusarium and Cordycipitaceae had a relatively close relative abundance and a balanced distribution in root samples and rhizosphere soil samples.【Conclusion】These results showed that root samples and rhizosphere soil samples had similar structure, the difference among species and abundances was not obvious, but there were many shared dominant communities in root and rhizosphere soil of P. hirsutissimum. [ABSTRACT FROM AUTHOR]

[Objective]This experiment aimed to discuss the mutagenic effects of the 60 Co-γ irradiation treatment on cutting seedlings of roses. [Method] Five 60 Co-γ doses were selected to irradiate the cutting seedlings of mses 4 Hiohgi’ and 4 Carola’, and then the survival rate, death rate, variation of the growth status of cutting seedlings were analyzed, to determine the relationship between radiation dose and death rate, as well as the medial lethal dose. [Results] With the increase of radiation dose, the survival rate decreased, while the death rate increased, there was a positive correlation between the radiation dose and death rate; the medial lethal doses of 4 Hiohgi’ and 4 Carola’ were 71 and 68 Gy, respectively. The radiation effect was more significant with the increase of radiation dose. The variation of plant was character variation, showing in leaves and flowers. [Conclusion] Different varieties of mses had different sensitivity to 60 Co-γ, the greater the radiation dose, the more significant the radiation effect, the medial lethal dose of different roses were different. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to understand the community composition of mycorrhizal fungi in Paphiopedilum hirsutissimun( Orchidaceae) and establish foundation for screening excellent strains, the optimal method for sterilization of plant materials surface with 75% ethanol and 10% NaClO was determined by the tissue isolation method and fungus was identified preliminarily based on morphological characteristics in this paper. The results showed that 75% ethanol for 8 min, 10% NaClO for 8 min were more suitable for roots sterilization. 63 strains fungi were isolated except that 8 strsains remained undetermined, other 55 strains were classified and belonged to 5 genera. The dominant groups of those mycorrhizal fungi were Fusarium and Daldinia, and Hypoxylon were firstly reported on Paphiopedilum hirsutissimun. Specifically, Tulasnella, a species of typical orchid mycorrhizal fungi, has been found. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)