[ Objective] This paper aimed to understand the biological information and function of the Heat shock transcription factor( Hsf) in apple. [ Method]36 Hsfs were retrieved from the apple genome GDH13 vl. l,confirmed by Pfam and NCBI Conserved Domain Database,and then,TBTools,ExPASy,MEME and other software were used to further analyze33 genes with complete Hsf domain. [ Result]The proteins encoded by these genes contained 189 - 530 amino acids, with molecular weights of 21 703. 71 - 58 972. 96, and isoelectric points of 4. 55 - 8. 58. The prediction of subcellular localization showed that MD00G1095900 and MD02G1171800 could be located in chloroplast/nucleus, MD05G1313700 could be located in nucleus/mitochondria,and other Hsf were localized in the nucleus. Chromosome mapping and synteny analysis showed that there was no tandem duplication of apple Hif genes, but there were segmental duplication in 16 pairs of 24 apple Hif genes. Under saline-alkali stress, the expression of most Hif genes were significantly down-regulated. Protein interaction network analysis showed that MD03 G1258300 interacts with heat shock protein, superoxide dismutase copper chaperone, protein phosphatase 2C, and sucrose unfermentable 1-associated protein kinase 'Y regulatory subunit. MD15G1283700 interacts with heat shock protein, IAA amino acid hydrolase and alkaline leucine zipper. [Conclusion] Hsfs of apple may play an important role in regulating the response to saline-alkali stress, which provides some theoretical reference for further study of biological functions of Hsfs in apple. [ABSTRACT FROM AUTHOR]