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针对传统部分包覆组合柱（PEC柱）中腹板易变形屈曲，与混凝土协同作用较弱、易分离等问题，提出将腹板形式由平钢板改为波形钢板的波形腹板PEC柱。考虑强弱轴方向、波形腹板方向、横向系杆间距、偏心距4个关键因素，设计10个波形腹板PEC柱试件并进行偏心加载试验。试验结果表明：波形腹板PEC柱在偏心受压下的破坏形态基本一致，均表现为受压侧翼缘鼓曲变形和混凝土压碎失效。试件在强轴方向的承载力较弱轴方向最大可提高61.20%；延性最大可提升63.06%；竖向波形腹板试件的承载力和初始刚度均高于横向波形腹板试件；较小偏心受压试件的承载力高于较大偏心受压试件；减小横向系杆间距，试件达到峰值荷载后延性性能提高，最大可提升75.16%。通过ABAQUS有限元软件建立波形腹板PEC柱偏心受压模型，在与试验结果进行对比验证的基础上，结合已有PEC柱试件的相关规范，拟合出竖向波形腹板PEC柱压弯承载力计算公式，理论值与试验值相差10%以内。

型钢混凝土柱是一种抗震性能优越的组合构件，经常被用于高层或超高层建筑中。近年来为了满足建筑物美观及室内装饰装修的要求，国内外学者针对型钢混凝土异形柱开展了相关研究，其中最常见的异形柱截面类型为十字形、L形和T形。以收集的型钢混凝土异形柱抗震性能试验为基础，对型钢混凝土异形柱的截面曲率进行分析，通过弯矩-曲率曲线研究曲率与构件变形性能的关系，确定异形柱屈服曲率和极限曲率的计算方法，利用截面屈服曲率和极限曲率得出位移延性系数。通过参数分析，研究构件的截面曲率变化情况，并通过参数拟合，提出适用于3种截面类型的型钢混凝土异形柱屈服曲率和极限曲率的简便计算公式，该公式可为实腹式型钢混凝土异形柱的变形计算以及抗震设计提供参考。

Objective Methods Forty adult periodontitis patients were involved(twenty in the test group and twenty in the control group, randomly). All patients received systematic periodontal examination, recording and calculating percentage of sites with plaque, probing depth and bleeding on probing, and implement routine oral hygiene instruction at the first visit. The test group received additional plaque staining and individual oral hygiene instruction. Both groups completed supragingival scaling and polishing at the first visit. After 1 week, subgingival scaling and root planning were completed. After 6 weeks, re-evaluation was performed, and oral hygiene and periodontal clinical indicators were recorded and compared. Results The percentage of plaque in test group and control group decreased by (56. 21 ± 14. 64)% and (43. 82 ± 11. 01)% respectively after treatment; The percentage of calculus decreased by(33. 60 ± 11. 01)% and (21. 28 ± 11. 96)% respectively compared with that before treatment. There were also statistically significant differences in the percentage of plaque and calculus between the two groups before and after treatment(P＜0. 001). After treatment, the probing depth of the two groups decreased by (1. 61 ± 0. 96) mm and (0. 86 ± 0. 69) mm, respectively; The percentage of bleeding after probing in the two groups decreased by (49. 51 ± 14. 67) % and (36. 70 ± 15. 40) %, respectively, compared with that before treatment. There were also statistically significant differences in the probing depth and the percentage of bleeding on probing between the two groups before and after treatment, and between the two groups(P＜0. 001). Conclusion The auxiliary application of plaque staining is helpful to improve the oral health status of patients with periodontitis and the clinical performance of periodontal non-surgical therapy. T o evaluate the clinical effect of plaque staining-assisted routine oral health education on oral hygiene of and clinical performance of periodontal non-surgical therapy for patients with periodontitis. (目的 评估菌斑染色辅助常规口腔卫生宣教方法的临床效果。方法 选取40例拟行牙周基础治疗的成年牙周炎患者, 随机分入试验组和对照组, 各20例。首诊均接受系统牙周检查, 记录和计算菌斑、牙石百分比、探诊深度和探诊后出血并实施常规口腔卫生宣教。试验组额外应用菌斑染色并接受个性化口腔卫生指导。两组均于首诊完成龈上洁治和抛光, 1周后分区段完成龈下刮治和根面平整, 6周后进行再评估, 记录并对比口腔卫生情况和牙周临床指标。结果 治疗后试验组与对照组菌斑百分比较治疗前分别降低(56. 21±14. 64)%和(43. 82±11. 01)%; 牙石百分比较治疗前分别降低(33. 60±11. 01)%和(21. 28±11. 96)%, 两组菌斑和牙石百分比治疗前后的差异和两组间降低值的差异均有统计学意义(P＜0. 001)。治疗后两组探诊深度较治疗前分别降低(1. 61±0. 96) mm和(0. 86±0. 69) mm; 两组探诊后出血百分比较治疗前分别降低(49. 51±14. 67)%和(36. 70±15. 40)%, 两组探诊深度和探诊后出血百分比治疗前后的差异和两组间降低值的差异均有统计学意义(P＜0. 001)。结论 辅助应用菌斑染色有助于改善牙周炎患者的口腔卫生状况和牙周基础治疗疗效。)

Objective To explore the relationship between the expression of Toll like receptor 4 (TLR4) and Janus protein tyrosine kinase 3 (JAK3) genes and the imbalance of helper T cell 17 (Th17)/regulatory T cells (Treg) in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS). Methods A total of 101 AS patients were selected as the AS group. According to the AS Disease Activity Index (BASDAI) score, patients were divided into the AS stable phase (ASS) group (＜4 points, 42 cases) and the AS active phase (ASA) group (≥ 4 points, 59 cases). Additionally, 50 healthy volunteers who underwent physical examinations at our hospital during the same period were selected as the control group (health group). Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to determine the mRNA expression of TLR4 and JAK3 in PBMCs, and the proportion of serum Th17 and Treg was measured and Th17/Treg levels were calculated. Results The levels of TLR4 mRNA and JAK3 mRNA in PBMCs of peripheral blood were higher in the AS group than those in the health group (P＜0.05). The levels of Th17, proportion and Th17/Treg in peripheral blood were higher in the AS group than those of the health group (P＜0.05). Higher levels of TLR4 mRNA, JAK3 mRNA, Th17, and Th17/Treg were risk factors for AS, while higher level of Treg was protective factors for AS. TLR4 mRNA and JAK3 mRNA in AS patients were positively correlated with Th17 ratio and Th17/Treg ratio, and negatively correlated with Treg ratio (P＜0.05). Conclusion TLR4 and JAK3 genes are highly expressed and Th17/Treg is unbalanced in PBMC of AS patients. The high expression of TLR4 and JAK3 genes is closely related to Th17/Treg imbalance in AS patients. [ABSTRACT FROM AUTHOR]

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骨质疏松症 (OP) 是一种以骨量减少, 骨微结构退化, 骨脆性增加及易骨折为特征的全身骨骼疾病. 目前 其影像学检查技术主要包括基于骨密度的影像学检查技术［双能线吸收检测 (DAX) 技术, 定量 CT (QCT), 基于X射 线吸收仪的三维重建 (3D-DXA) 技术] 及基于骨超微结构的影像学检查技术 (骨小梁评分, 高分辨外周定量 CT与 基 于个体小梁分割技术) . 应用于 OP 的诊断并在世界范围内形成共识的影像学技术只有双能线吸收检测法 (DAX). 然而DXA测量的面积平均骨密度是骨皮质, 骨松质骨与软组织平面投射经过特定软件计算而得, 易受体型, 退行性 关节病等因素影响. QCT 则能很好地解决上述的问题, 其可以区分骨皮质与骨松质, 测量感兴趣区的单一骨类的体 积平均骨密度 (vBMD), 在反映目标骨真实骨量的方面优于 DXA, 因此, 其在 OP 的诊断与骨折预测等方面优于 DXA, 但其高辐射量限制了重复测量的可能性. 3D-DXA 能够对 2D-DXA 图片进行三维重建从而获取 vBMD, 安全 性与经济效益等方面均优于QCT. 然而骨量并不能完全解释骨折的发生, 骨微结构在骨中所扮演的角色越发被重 视, 相应的影像学技术诸如: 骨小梁评分, 高分辨外周定量 CT 与基于个体小梁分割等也相继出现. 骨微结构相关的 量化参数的下降与骨强度的下降相关, 相关参数与骨折独立相关, 能够有效地提高 OP 的诊断率, 具有重要的临床意 义, 但目前主要仅局限在实验研究阶段. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Objective To observe the effects of Fusobacterium nucleatum and microRNA-497(miR-497) on the proliferation, invasion and migration of colorectal cancer cells, and to analyze their regulatory relationships. Methods Colorectal cancer cell line HCT116 was transfected with miR-497 overexpression plasmid miR-497 mimic and was randomly divided into the infection index (MOI) 100 group, MOI 200 group, MOI 500 group, and MOI 1 000 group. They were co-cultured with Fusobacterium nucleatum with MOI of 100, 200, 500 and 1 000, respectively. Real-time fluorescent quantitative PCR was used to detect miR-497 in each group. The treatment concentration of the bacteria with the lowest expression of miR-497 was selected as the subsequent action concentration of Fusobacterium nucleatum. HCT116 cells were randomly divided into the control group, miR-497 over-expression group, miR-497 down-expression group, miR-497 over-expression negative control group and miR-497 down-expression negative control group, respectively. Cells in the control group were cultured without treatment. Cells in the miR-497 over-expression group, miR-497 down-expression group, over-expression negative control group, and down-expression negative control group were transfected with miR-497 mimics, miR497 inhibitor, mimic NC, and inhibitor NC, respectively, by Lipofectamine 2000. Cell proliferation ability was observed by CCK-8 assay, cell invasion ability was observed by Transwell assay, and cell migration ability was observed by Scratch healing assay. HCT116 cells were randomly divided into the control group, miR-497 over-expression group, overexpression negative control group, and over-expression + Fusobacterium nucleatum group, respectively. Cells in the control group were cultured without treatment, and cells in the miR-497 over-expression group were transfected with miR-497 mimic, while cells in the over-expression negative control group were transfected with mimic NC. After transfecting miR-497 mimic, cells in the overexpression + Fusobacterium nucleatum group were co-cultured with selected concentration of Fuso⁃ bacterium nucleatum. The cell proliferation ability was observed by CCK-8 assay, the cell invasion ability was observed by Transwell assay, and the cell migration ability was observed by Scratch healing assay. Results The miR-497 expression was as follows: MOI 100 group >MOI 200 group >MOI 500 group >MOI 1 000 group (all P<0. 05), and MOI 1 000 was selected as the subsequent action concentration of Fusobacterium nucleatum. Cell proliferation rate, the number of invasive cells and migration rate were as follows： miR-497 down-expression group > control group, overexpression negative control group, down-expression negative control group > miR-497 over-expression group (all P<0. 05). Cell proliferation rate, the number of invasive cells, and migration rate were as follows: miR-497 overexpression group < overexpression + Fusobacterium nucleatum group < control group, overexpression negative control group (all P<0. 05). Conclusion Fusobacterium nucleatum can promote the proliferation, invasion and migration of colorectal cancer cells through negative regulation of miR-497. [ABSTRACT FROM AUTHOR]

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The aim of this experiment was to understand the effect of different heat-moisture treatment conditions on the mechanical behavior of feed pelleting process. Using the self-built single pellet press (SPP) test platform, the pellet compression molding tests of 15%, 20% and 25% moisture feed after 1 min, 3 min and 5 min heat treatment at 70 °C, 80 °C; and 90 °C are completed. The compression curve of feed can be divided into four stages by using the stress gradient method, i.e. the press bar into the die stage, the pressing stage, the yield stage and the compaction stage. A nonlinear elasto-plastic visco rheological model is established by parallel connection of strain strengthened nonlinear elastic element and Newtonian viscous body. The coefficient of determination R² of the model is greater than 0.98, and the average relative percentage error is 0.52%-7.13%. The analysis of the influence of heat-moisture treatment conditions on the rheological model parameters of the compaction stage indicates that: with the increase of feed moisture and heat treatment temperature, the proportion of plastic deformation in feed compression deformation increases. As the heat treatment time prolongs, the proportion of elastic deformation increases. The increase in feed moisture is beneficial for the expression of binder components such as starch and protein in the feed after heat-moisture treatment, and the compression characteristics of high moisture feed are less sensitive to temperature. [ABSTRACT FROM AUTHOR]

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Objective To establish a rat model of hepatic encephalopathy and a fecal bacterial transplantation (FMT) model, and explore the changes in the intestinal flora of rats with hepatic encephalopathy after bacterial transplantation. Methods Thirty SD rats were selected, and a rat model of hepatic encephalopathy was established using carbon tetrachloride combined with ethanol. The rats were randomly divided into 3 groups, group A was the normal control group, group B was the hepatic encephalopathy model group, and group C was the FMT group, with 10 rats in each group. Group B was given the same dose of normal saline by gavage, and group C was given FMT (gavage at 200 μL/d, and each milliliter of bacterial solution contained 1.5×1011 bacteria). The survival conditions of rats were observed every day. Rat intestinal feces were collected for metagenomic sequencing. Results A total of 9 rats died during the experiment, and no rats died in group A. Before and after FMT, the weight of rats in group C increased significantly after transplantation. Through metagenomic sequencing, it was found that group C can effectively improve the diversity and abundance of bacterial species compared with group B; compared with group A, the content of Proteobacteria in groups B and C was significantly increased, and the abundance of Bacteroidetes was significantly decreased. Conclusion FMT can change the structure of intestinal flora in rats with hepatic encephalopathy. [ABSTRACT FROM AUTHOR]

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