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针对现有架空配电线路故障离线定位方法定位可靠性与精度不足的问题，建立适用于阻抗特征分析的线路等效模型，通过分析故障线路不同检测点处的零序阻抗角差异，提出一种基于卫星对时的故障离线式定位方法。该方法向停电隔离后的故障线路首端注入频率、相位恒定的交流电压信号，并手持信号接收装置进行人工巡线，利用卫星对时完成信号接收与注入装置间的同步采样，在不采集电压的情况下，即可实现检测点零序阻抗角的计算，进一步通过比较不同检测点处零序阻抗角差异，确定故障分支及故障点精确位置。分析并明确了注入信号频率、故障点过渡电阻与时钟同步误差对故障定位精度产生的影响。数字仿真结果表明该方法可有效实现故障的精确定位。

Objective·To establish a mouse model with dormant cancer and no obvious metastasis by combining the preimmune strategy with the mVenus-p27K－ cell G0 phase indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system. Methods·The KPC1199 mouse pancreatic cancer cell line was transfected with the mVenus-p27K－ cell G0 phase indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system to construct a stable expression cell line, KPC1199-PDL. After being cultured in the serum-free condition, KPC1199-PDL cells were sorted into mVenus (+) cells and mVenus (-) cells by flow cytometry, and the expression of G0 phase-related genes was verified by real-time fluorescence quantitative PCR (qPCR). Sensitivity of KPC1199-PDL cells to diphtheria toxin (DTX) and ganciclovir (GCV) was evaluated by CCK-8 assay. A transsplenic portal vein-hepatic metastasis model was constructed in wild-type C57BL/6 mice to validate the function of KPC1199-PDL cells in vivo by immunofluorescence technology. The KPC1199-PDL cells were injected subcutaneously into C57BL/6 mice, followed by in situ injection of DTX and GCV to ablate subcutaneous tumors 5 d later, to obtain preimmunized mice. The transsplenic portal vein-hepatic metastasis models were constructed in these mice. Bioluminescence imaging was used to evaluate subcutaneous tumor ablation and hepatic metastasis in the mice, and immunofluorescence assay was used to detect the distribution and dormant state of tumor cells in the livers of preimmunize mice. Results·The three tool systems were stably expressed in KPC1199-PDL cells, and their proliferative ability was not affected. In the serum starving condition, some KPC1199-PDL cells expressed the mVenus protein, indicating entry into the G0 phase; the mVenus (+) cells sorted out by flow cytometry exhibited significantly higher expression of G0 phase-related genes (all P<0.05) and significantly lower expression of the proliferation-related gene compared with mVenus (-) cells (P<0.05). The CCK-8 assay demonstrated high sensitivity of KPC1199-PDL cells to DTX and GCV. In vivo experiments confirmed that KPC1199-PDL cells could be effectively traced through tdTomato protein expression, and could indicate entry into the G0 phase through mVenus protein expression. Following subcutaneous tumor implantation and drug ablation, preimmunized mice were successfully obtained. In the subsequent transsplenic portal vein-hepatic metastasis model, no metastatic signals were observed in the liver by bioluminescence imaging, but single or small clusters of G0 phase tumor cells expressing both mVenus and tdTomato, not expressing the proliferation marker Ki67, were detected in liver tissue sections by immunofluorescence analysis. Conclusions·A recognizable and traceable dormant cancer model is constructed with the combination of the preimmune mouse model of pancreatic cancer, the mVeneus-p27K－ indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system. [ABSTRACT FROM AUTHOR]

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Objective To investigate the impact of ephedrine on knee osteoarthritis (KOA) in rats by regulating AMPK/NF-κB signaling pathway. Methods KOA rat models were established and grouped into Sham group, KOA group, Ephedrine group (40.0mg/kg), and Ephedrine group (40.0mg/kg)+Compound C group (0.2mg/kg). After drug grouping intervention, the knee joint width and pain threshold of rats were measured respectively,the pathological changes of cartilage tissue were observed with safranine O-solid green staining and Mankin score was performed,the levels of TNF-α, IL-1β and COX-2 in synovial fluid were detected by ELISA,the expression of IL-1β and MMP-13 proteins in cartilage tissue was detected by immunohistochemistry,Western blot was applied to detect the level of AMPK/NF-κB pathway related proteins. Results Compared with Sham group, the cartilage tissue of KOA group was seriously damaged,the knee joint width, Mankin score, levels of TNF-α, IL-1β, COX-2 in joint fluid, and levels of IL-1β, MMP-13, and p-NF-κB p65/NF-κB p65 in cartilage tissue were significantly higher, and the pain threshold and the level of p-AMPK/AMPK in cartilage tissue were significantly lower (P<0.05);compared with KOA group, the pathological damage of cartilage tissue in Ephedrine group was reduced,the knee joint width, Mankin score, levels of TNF-α, IL-1β, COX-2 in joint fluid, and levels of IL-1β, MMP-13, and p-NF-κB p65/NF-κB p65 in cartilage tissue were significantly lower, and the pain threshold and the level of p-AMPK/AMPK in cartilage tissue were significantly higher (P<0.05); compared with the Ephedrine group, the injury of cartilage tissue in the Ephedrine+Compound C group was aggravated,the knee joint width, Mankin score, levels of TNF-α, IL-1β, COX-2 in joint fluid, and levels of IL-1β, MMP-13, and p-NF-κB p65/NF-κB p65 in cartilage tissue were significantly higher, and the pain threshold and the level of p-AMPK/AMPK in cartilage tissue were significantly lower (P<0.05). Conclusion Ephedrine can inhibit NF-κB signal pathway, inhibit inflammatory reaction and improve cartilage injury in KOA rats by activating AMPK pathway. [ABSTRACT FROM AUTHOR]

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Objective To summarize the clinical efficacy of programmed management for H-type congenital tracheoesophageal fistula (H-TEF). Methods From March 2017 to March 2022, clinical data were retrospectively reviewed for 10 H-TEF children under programmed management.Six neonates developed recurrent cough after feeding and 4 infants had recurrent pneumonia.One case was associated with congenital heart disease and another had hemivertebra deformity of thoracic vertebra.After a definite diagnosis was confirmed by esophagography and/or fiberbronchoscope, gastric tube feeding was initiated.After inflammatory parameters normalized completely or partially and pneumonic change disappeared on chest film, a catheter was placed in fistula under a guidance of fiberbronchoscope.The position of fistula was confirmed by bedside radiography; H-TEF was repair through a cervical approach when fistula was located above T2 level and thoracoscopically below T2. Results Seven cases were confirmed by esophagography and 3 cases by fiberbronchoscope.Catheter was successfully deployed in fistula in all cases before surgery.Seven cases of fistula above T2 were repaired through a right cervical route and 3 cases of fistula below T2 underwent thoracoscopy.Feeding through a gastric tube resumed the next day and esophagography 1 week later.There was no leakage or recurrence during a follow-up period of (8-60) month.Three cases of hoarseness occurred after cervical surgery and recovered at (1-6) month.No dysphagia or recurrence was noted. Conclusion An integrated strategy for H-TEF yields satisfactory outcomes under programmed management. [ABSTRACT FROM AUTHOR]

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