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Copyright of Chinese Journal of Pathophysiology is the property of Jinan University, School of Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We aimed to analyze the differential expression of urinary exosome miRNAs in diabetes kidney disease (DKD) patients with dampness-heat syndrome in chronic kidney disease Ⅲ to Ⅳ stage by small RNA sequencing technology. Methods This study was conducted at the First Affiliated Hospital of Anhui University of Chinese Medicine from April 2021 to April 2022. In total, 80 subjects were enrolled, including 40 DKD patients with dampness-heat syndrome and 40 healthy controls. The urinary exosomes of 10 subjects in both groups were selected for miRNA high-throughput sequencing, and selected the differentially expressed miRNAs were selected according to log2(Fold Change) ≥1 and q value < 0. 05. Differentially expressed miRNAs were selected by the following criteria: the mean expression level ( TPM) in at least one group was > 2, log2(fold change) ≥ 2, and at least one sample in the DKD group has TPM> 0 and verified by quantitative RT-PCR. Results By comparing the 80 subjects’ data to the database, 302 miRNAs expressed in healthy controls and 386 miRNAs expressed in DKD patients. In total, 97 differentially expressed miRNAs were screened, in including 84 upregulated and 13 downregulated miRNAs. Of these, 10 miRNAs were selected for verification. RTPCR result showed that the expression levels of miR-664a-5p, miR-320b, and miR-199a-5p were significantly different between the two groups(P<0. 01). The areas under the ROC curve of miR-664a5p, miR-320b, and miR-199a-5p in the diagnosis of dampness-heat syndrome in DKD patients were 0. 886, 0. 923, and 0. 761, respectively. Conclusion We found differences in miRNA expression levels in urinary exosomes between DKD patients with dampness-heat syndrome and controls. The urinary exosome levels of miR-664a-5p, miR-320b, and miR-199a-5p can be used for the diagnosis of DKD patients with dampness-heat syndrome. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oncology is the property of Chinese Journal of Oncology / Zhonghua Zhongliu Zazhi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the influences of Qingshen (Kidney-Clearing) Granules,with effects of clearing heat,resolving dampness and dispelling stasis,on expressions of Janus-activated kinase 2 (JAK2) and signal transducers and activator of transcriptions 3 (STAT3) in Janus-activated kinasesignaltransducer and activator of transcription (JAKKSTAT) signaling pathway mediated by Leptin in patients with chronic renal failure (CRF) -damp-heat pattern,and investigate the mechanism of Qingshen Granules in treatment of CRF. Methods The patients with CRF-damp-heat pattern (n = 68) were randomly divided into treatment group and control group (each n = 34). The study was completed actually in 60 patients, and 29 in treatment group and 31 in control group. All 2 groups were given basic treatment of Western medicine,and treatment group was additionally given Qingshen Granules (thrice daily and 1 bas every time) for 12 weeks. The clinical efficacy and changes of serum creatinine (SCr), estimated glomerular filtration rate (eGFR),serum Leptin,JAK2 and STAT3 were observed in 2 groups. Results The clinical efficacy was 86. 21% (25/29) and total effective rate of TCM patterns was 82.76% (24/29) in treatment group,and 58. 06% (18/31) and 54. 84% (17/31) in control group (P <0. 05). After treatment,the level of SCr decreased significantly (P <0. 01) and level of GFR increased significantly (P <0.01) in treatment group compared with those before treatment,which were superior to those in control group at the same term (P <0. 05). After treatment,the level of Leptin,and expressions of JAK2 and STAT3 decreased significantly (P <0.01) in treatment group compared with those before treatment,which were superior to those in control group at the same term (P <0. 05 or P < 0. 01). Conclusion Qingshen Granules can reduce the level of Leptin and expressions of JAK2 and STAT3,inhibit the activation of JAIKSTAT signaling pathway mediated by Leptin, relieve clinical symptoms and improve kidney function in patients with C RF - damp - heat pattern. [ABSTRACT FROM AUTHOR]

Objective This multicenter, randomized-controlled clinical trial was designed to study the clinical efficacy of Qingshen Granule, formulated by TCM therapy principle of clearing heat ( qingre) , eliminating dampness ( huashi) and removing blood stasis ( quyu) , on chronic renal failure patients with damp-heat pattern. Methods A total of 282 patients eligible were divided into treatment group ( n = 136) and control group ( n =146). All patients were treated with conventional therapy, in addition, patients of treatment group took orally Qingshen Granule at a dose of 10 g, three times daily for consecutive 12 weeks. Results The total clinical curative effective rate of the treatment group (79.41% ) was significantly higher than that of the control group ( 67. 12% ) , and there were significant differences between the two groups( P <0. 05 ). The clinical effect of TCM pattern in the treated group ( 86. 76% ) was significantly higher than that in the control group (59. 59% ) .and there were significant differences between the two groups (P <0. 01 ). After treatment, the levels of U-pro/24h and Scr were obviously lower (P <0. 01) , while eGFR was obviously higher ( P < 0. 01 ) , superior to the control group ( P < 0. 05 ). Conclusion On the basis of conventional therapy, the satisfactory clinical efficacy of Qingshen Granule on chronic renal failure patients with damp-heat pattern was proved by ameliorating clinical symptoms, decreasing TCM pattern scores, reducing urinary protein and protecting renal function. [ABSTRACT FROM AUTHOR]