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【目的】研究microRNA-30a-5p（miR-30a-5p）对人宫颈癌Hela细胞上皮-间质转化功能的影响及其相关机制。【方法】宫颈癌Hela细胞株分别转染目的mir的模拟物和阴性对照模拟物，分别以30a-5p组、NC组命名并标记细胞。同时，以未经过处理的Hela细胞作为对照（Control组）。分别用逆转录-聚合酶链反应法检测各组宫颈癌细胞的miR-30a-5p含量。Transwell实验检测3组细胞迁移能力和侵袭能力。Western-blot法检测3组细胞神经-钙粘素（N-cadherin）、α-连环蛋白（α-Catenin）和泛素水解酶22（USP22）表达水平。运用生物信息学方法预测miR-30a-5p的靶基因。采用Western blot法检测USP22过表达对miR-30a-5p抑制EMT的拮抗作用。双荧光素酶实验检测miR-30a-5p与USP22的关系。建立皮下移植瘤模型观察miR-30a-5p的体内作用。【结果】30a-5p组宫颈癌细胞miR-30a-5p的表达水平明显上调，表达水平为Control组的853.82（862.26~843.11）倍（P＜0.01）。30a-5p组侵袭细胞数量8.17（8.32~8.03）明显低于Control组（P＜0.01）。30a-5p组细胞N-cadherin蛋白的细胞内含量明显下降，α-Catenin蛋白的细胞内含量明显上升，USP22蛋白表达量明显降低。合并USP22过表达处理的30a-5p组宫颈癌细胞中N-cadherin蛋白表达量明显升高，α-Catenin蛋白表达量明显降低。双荧光素酶检验结果显示USP22为miR-30a-5p的下游靶基因（P＜0.01）。30a-5p组皮下移植瘤明显小于Control组（P＜0.01）。与Control组肿瘤组织相比，30a-5p组肿瘤组织miR-30a-5p的相对含量升高，USP22蛋白含量降低，N-cadherin蛋白的含量降低，α-Catenin蛋白含量升高。【结论】miR-30a-5p在宫颈癌Hela细胞中，可能通过靶向识别下游靶基因USP22，进而抑制其翻译。最终实现对宫颈癌细胞EMT过程的抑制。

为鉴定从临床获得的病死成年兔中分离到的一株革兰氏阴性杆菌,应用VITEK 2 Compact全自动微生物分析系统仪完成细菌生化鉴定及药敏试验,并对16S rRNA基因测序鉴定,通过MegAlign（Lasergene7.1）及Phylogenetic（Lasergene7.1）绘制进化树及同源性比较以了解该株细菌的遗传进化关系。结果表明此株细菌是支气管败血波氏杆菌,并与BX640428等序列同源性高达100%。生化试验及药敏试验结果表明谷氨酰芳胺酶pNA试验、L-脯氨酸芳胺酶试验、酪氨酸芳胺酶试验、尿素酶试验、柠檬酸盐（钠）试验、L-乳酸产碱试验、琥珀酸盐产碱试验为阳性,对头孢替坦、头孢唑啉、呋喃妥因具有耐药性。

1989年对哈尔滨市特产所饲养的6只健康成年雉鸡消化系统进行了系统解剖,用肉眼及OLYMPUS解剖镜进行观察,结果表明:雉鸡腺胃粘膜乳头较多,肌胃很发达,小肠较长,十三指肠绒毛长而密集、直肠构造与小肠相似,盲肠较发达,肝脏相对较轻。同时与鸡形目雉科中家鸡、鹌鹑及松鸡科部分鸟类进行了比较,它们之间有许多相似之处,但也各有特点。

环颈雉（phasianus colchicus torguatus）属鸡形目、雉科。它是主要的猎禽,无限制的狩猎,使自然界中的数量急剧减少,为了保护和利用野生资源,提高人工繁养能力,我们对雌、雄各3只环颈雉进行了生殖系统解剖,并与同科中的家鸡、鹌鹑进行了生殖系统形态比较,将为环颈雉的繁养提供参考。

成年东北马鹿肠的组织结构与牛羊等反刍动物基本相似。其主要特点是：十二指肠有其特有的十二指肠腺，肠腺由上皮细胞构成，周围有纵行肌。肠壁的肌层发达。

本文采用成年ＳＤ大鼠经冠状动脉口注入内皮素（ＥＴ，６００ｐｍｏｌ／ｋｇ）和降钙素基因相关肽（ＣＧＲＰ，１７０ｐｍｏｌ／ｋｇ），观察ＣＧＲＰ对ＥＴ引起大鼠心律失常作用的影响。发现ＥＴ可诱发心律失常的发生，ＣＧＲＰ对ＥＴ引起的心律失常具有协同作用。结果提示：作用方式可能是对心肌造成的缺血／再灌注损伤使细胞内钙超载和氧自由基大量产生。

采用酸性。α－醋酸萘醋酶（ＡＮＡＥ）反应标记淋巴细胞的细胞化学方法，石蜡连续切片，分别做ＡＮＡＥ染色和Ｈ·Ｅ、Ｗｉｌｄｅｒ法染色，对艾鼬脾脏进行组织结构及Ｔ淋巴细胞分布的研究。结果表明：艾鼬脾脏被膜较薄，被膜下淋巴组织呈弥散性分布．脾小梁不发达：白髓是以中央动脉周围淋巴鞘和脾小结的形式散布在红髓之间；牌小结未见生发中心；边缘区不发达，淋巴细胞排列疏松，在边缘区内分布有较多椭球，椭球体积较小；红髓发达，脾窦宽大。ＡＮＡＥ阳性反应淋巴细胞主要分布在中央动脉周围淋巴鞘、脾小结、边缘区和椭球内也有少量分布。

采用石蜡和冰冻切片方法，进行３Ｍ·Ｅ和Ｗｉｌｄｅｒ染色，并以酸性α－醋酸萘醋酶（ＡＮＡＥ）法显示Ｔ淋巴细胞的分布。结果表明：周岁黑熊（Ｓｅｌｅｎａｒｃｔｏｓｔｈｉｂｅｔａｎｕｓ）脾脏小梁较多，脾窦发育不良．动脉周围淋巴鞘发达；鞘动脉位于脾索内，靠近边缘区；椭球厚为３０－４０μｍ。ＡＮＡＥ阳性反应的淋巴细胞主要分布于白髓的中央动脉周围淋巴鞘内，其次是脾小结外周，边缘区和椭球内。

用核酸的组织化学方法对梅花鹿巴氏杆菌病脾脏Ｔ．Ｂ淋巴细胞及其ＤＮＡ和ＲＮＡ的分布进行研究。结果表明：Ｔ－淋巴细胞主要分布在中央动脉周围淋巴鞘、脾小结外周、边缘区及椭球内。Ｂ－淋巴细胞主要存在于脾小结生发中心和脾索中。ＤＮＡ和ＲＮＡ的分布未见异常，即ＤＮＡ存在于淋巴细胞核，ＲＮＡ存在于淋巴细胞的胞浆中，脾脏被膜、小梁平滑肌肿胀，排列疏松，脾髓内含有大量血液。红髓发达，脾索中浆细胞增多，窦腔内网状细胞增殖，有大量黄血盐沉着。白髓不发达，被膜下有弥散性淋巴组织，生发中心不明显；中央动脉淋巴鞘发达。边缘区不明显。鞘毛细血管数量不多，体积也比较小。

肝脏（hepar or liver）是动物体内最大的消化腺,也是机体内一个非常重要的代谢器官,了解肝脏的结构,对掌握其复杂的生理功能有十分重要的意义。 1 试验材料和方法有的材料是在野外捕获而取材,有的是在某养殖场随机取材,把材料分别用10％中性福尔马林和Lillie氏AAF固定液中进行固定,石蜡切片,切片厚5～6μm,H·E和PAS过碘酸雪夫氏反应法进行染色,显微镜

麝鼠为珍贵的野生经济动物之一,自然状态下,具有顽强的生命力和抗病能力,人工养殖中,除易患巴氏杆菌病等几种少数疾病外,正常情况下很少患病。我们在临床中,发现一例巨大的甲状腺未分化癌,对今后麝鼠疾病的研究有一定意义,特作如下报告: 临床症状 1989年9月,黑龙江省某麝鼠养殖者发现所养麝鼠中,一只一岁雄性麝鼠在2个月时间内迅速消瘦,被毛粗乱,体重减轻,体表骨骼轮廓显露,行走无力,同时发现该鼠

为增强卵磷脂在食品领域的适用性，促进菜籽油凝胶的开发，以菜籽油为基料油，添加卵磷脂和山梨醇酐单硬脂酸酯（SMS）制备菜籽油凝胶，探究卵磷脂与SMS质量比（0∶ 10、1∶ 9、2∶ 8、3∶ 7和4∶ 6）对其理化性质、微观结构与氧化稳定性的影响。结果表明：卵磷脂与SMS质量比对菜籽油凝胶硬度和析油率具有显著影响（p< 0.05)，卵磷脂与SMS质量比为2∶ 8时菜籽油凝胶的硬度最高，析油率最低；菜籽油凝胶的弹性模量（G′）均大于黏性模量(G″)，表明全部形成凝胶结构，卵磷脂与SMS质量比为2∶ 8时具有最高的弹性模量和黏性模量；XRD、氧化稳定性、FTIR和DSC分析表明，卵磷脂比例的增加有利于菜籽油凝胶结晶和氧化稳定性的提升，但热力学稳定性降低，菜籽油凝胶网络分子间作用力以氢键为主。综上，卵磷脂与SMS复合作为凝胶剂可有效固化菜籽油成功构建菜籽油凝胶体系，其中卵磷脂与SMS质量比为2∶ 8时形成的菜籽油凝胶的凝胶特性最好。 To enhance the applicability of lecithin in the food field and promote the development of canola oil oleogels, canola oil oleogels were prepared by adding lecithin and sorbitan monostearate (SMS) to canola oil,and the effects of mass ratios of lecithin to SMS(0∶ 10, 1∶ 9, 2∶ 8, 3∶ 7 and 4∶ 6) on their physicochemical properties, microstructure and oxidation stability were investigated. The results showed that the mass ratio of lecithin to SMS had significant effects on the hardness and oil separation rate of canola oil oleogels (p

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Objective To introduce the revised Death Depression Scale and evaluate the reliability and validity of its Chinese version among clinical nurses. Methods The original scale was adapted into Chinese through translation, back-translation, cultural adaptation, and preliminary testing. A convenience sampling method was used to survey 667 nurses from a city in Guangdong province to assess the scale′s reliability and validity. Results The Chinese version of the Death Depression Scale includes 3 dimensions and 21 items. The Cronbach′s alpha coefficient for the scale was 0.842, and the test-retest reliability was 0.839. The content validity index was 0.820. Exploratory factor analysis extracted 3 common factors, with a cumulative variance contribution rate of 68.59%. Confirmatory factor analysis showed good model fit (χ²=3 710.997, P＜0.001; χ²/df=2.166; CFI=0.968; GFI=0.920; AGFI=0.898; RMSEA=0.053), and all factor loadings were greater than 0.4 and statistically significant. Conclusion The Chinese version of the Death Depression Scale demonstrates good reliability and validity among nurses. It is recommended for use in clinical settings and research to assess death-related depression in clinical nurses. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the intervention effectiveness of acceptance and commitment therapy (ACT) for patients with depressive disorders. Methods: A computer search of the Web of Science Core Collection, Pubmed, EMBASE, Cochrane Library, PsycINFO, CNKI, WanFang and WeiPu full-text databases (until October 23, 2023) was used to collect randomized controlled studies that used acceptance and commitment therapy for treating patients with depressive disorders. Using Stata 17 software, meta-analysis was performed on the literature data that met the inclusion criteria. Results: After screening, 11 studies remained for data extraction. The results of a random effects model indicated that ACT could significantly reduce depression levels in patients with depressive disorders (SMD 1.22, 95% CI: 2.050.40, P <0.01), and had a certain maintenance effect (SMD = 3. 59, 95% CI: 1.57-5.62, P<0.01). Conclusion: Acceptance and commitment therapymay could improve the level of depression in patients. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Cryptologic Research (2097-4116) is the property of Editorial Board of Journal of Cryptologic Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Lanthanide ions doped white-light upconversion materials have important applications in solid state lighting, liquid crystal display and information anti-counterfeiting. However, previous research mainly focus on inert matrix materials, which are difficult to significantly increase the concentration of sensitizer, limiting the emission intensity of white-light upconversion. Herein, a LiYbF4@LiYF4 core-shell nanostructure design is proposed. By using the sensitizing LiYbF4 nanocrystals as host doping with appropriate concentrations of Er3+ and Tm3+ activators, white-light upconversion emission is successfully achieved under 980 nm excitation. The LiYbF4 ∶Er/Tm@LiYbF4@LiYF4 ∶Nd core-shell-shell nanostructure is further designed to realize the white-light emission under 980 and 808 nm dual-channel excitation through using additional sensitizer Nd3+ . Finally, a package of both white-light upconversion nanoparticles and 940 nm near-infrared chips into LED device contributes to bright white-light emission by applying 300 mA current, showing great potential in white LED. [ABSTRACT FROM AUTHOR]

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目的: 在健康体检中通过CT、DR和肿瘤标识物对肺癌进行筛查, 对筛查结果进行分析研究, 评价各 种筛查价值. 方法: 本研究选取2020年1月至2023年11月健康体检中确诊肺腺癌785例为研究对象, 分析肺腺癌检出 线索, 包括CT、DR、肿瘤标志物CEA、Ca211等, 分析各种线索的检出阳性率并将其进行对比. 结果: CT检出率 435.67/10万, DR检出率8.75/10万, 两种对比差异明显, 经统计学计算, P < 0.001, 有显著统计学意义. 单独CEA检 出率1.61/10万, 单独Ca211检出率2.49/10万, 两种对比差异明显, 经统计学计算, P < 0.05, 有统计学意义. 结论: 筛 查早期肺癌胸部CT比DR优势显著, 可以有效提高肺癌检出率, 联合CEA、Ca211筛查可以提高肺腺癌发现率, 尤其 Ca211可以成为肺腺癌筛查的主要肿标. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Natural Disasters is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)