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Copyright of Journal of Earth Sciences & Environment is the property of Journal of Earth Sciences & Environment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the application value of Multi-Latex polygranular technique joint detection of kidney injury-related urinary microproteins in noninvasive diagnosis after renal transplantation. Methods Clinical data of 72 recipients undergoing renal transplantation were retrospectively analyzed. According to the level of serum creatinine (Scr), the recipients were divided into normal renal function group (group A, n=14), mild kidney injury (group B, n=37), and severe kidney injury group (group C, n=21). 20 healthy volunteers were selected as the healthy control group (HC group). The contents of urinary retinol binding protein (RBP), microalbumin (mAlb), IgG, transferrin (TRF), α1-microglobulin (MG), and β2-MG of subjects in each group were detected using the Multi-Latex polygranular technique. The correlation between urinary microproteins and Scr, blood urea nitrogen (BUN) was analyzed. The differences of urinary microproteins in each group were compared. And the diagnostic value of single and joint detection of urinary microproteins was evaluated. Results Six kinds of urinary microproteins in HC group and group A were significantly lower than those in group B and group C, and six kinds of urinary microproteins in group B were significantly lower than those in group C (all P<0.01). Six kinds of urinary microproteins in renal transplant recipients were positively correlated with BUN. RBP, mAlb, α1-MG, and β2-MG were positively correlated with Scr. The correlations were statistically significant (P<0.001-0.05). The diagnostic value of joint detection of urinary microproteins is better than the detection of single index, among which TRF+mAlb+RBP+α1-MG quadruple detection had the highest diagnostic value. Conclusions Six kinds of urinary microproteins can be used as specific indicators to reflect graft renal function. The polygranular technique can simultaneously detect its contents and achieve noninvasive diagnosis. The diagnosis based on TRF+mAlb+RBP+α1-MG quadruple detection is expected to further improve the noninvasive diagnosis system after renal transplantation. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of umbilical cord mesenchymal stem cells with positive human leukocyte antigen (HLA)-G on inducing the production of regulatory T cells (Treg) in vitro. Methods Umbilical cord mesenchymal stem cells were isolated from umbilical cord of neonates. PEGFP-N1-HLA-G plasmid was transfected into the human umbilical cord mesenchymal stem cells by liposome transfection, as PEGFP-N1-HLA-G group. PEGFP-N1 empty vector plasmid was transfected into the human umbilical cord mesenchymal stem cells, as PEGFP-N1 group. The human umbilical cord mesenchymal stem cells without empty vector under the same conditions were set as blank control group. Markers of the umbilical cord mesenchymal stem cells were detected using flow cytometry. The expression of HLA-G protein in each group of cells was identified by Western Blot. After mixed-culturing with CD4+T cells in peripheral blood of healthy subjects for 24 h and 48 h, the proportion of CD4+ CD25+ Foxp3+Treg in total T cells of each group was detected by flow cytometry. Results CD45, CD34 and HLA-DR presented negative expression on umbilical cord mesenchymal stem cells, while CD29, CD44 and CD105 presented positive expression. HLA-G protein could be expressed in the PEGFP-N1-HLA-G group, which had statistically significant difference compared with the blank control group and PEGFP-N1 group (both P<0.01). After PEGFP-N1-HLA-G group and CD4+T cells were mixed-cultured for 24 h and 48 h, CD4+ CD25+ Foxp3+Treg accounted for (15.3±1.9)% and (14.3±2.1)% of the total T cells respectively, both of which presented statistically significant difference compared with the blank control group and PEGFP-N1 group (all P<0.05). Conclusions Umbilical cord mesenchymal stem cells with HLA-G gene modified can effectively induce the production of CD4+ CD25+ Foxp3+Treg in vitro. [ABSTRACT FROM AUTHOR]

Objective To investigate the change rules and its significance of erythrocytes surface molecule CD35, CD58 and CD59 expression in recipients infected with cytomegalovirus (CMV) after renal transplantation. Methods Eighty-two recipients undergoing allogeneic renal transplantation were selected and divided into the negative (n=21) and positive CMV groups (n = 61) based on the qualitative detection of CMV-pp65 antigen in peripheral blood. According to the results of CMV-pp65 (+) leucocyte count, all 61 patients in positive CMV group were further divided into low (n = 55) and high active infection subgroups (n = 6). Healthy adults were recruited into the normal control group (n = 30). The expression levels of CMV-pp65 antigen, erythrocytes surface molecule CD35, CD58 and CD59 were measured by flow cytometry. Results Compared with normal control group, the expression levels of erythrocytes surface molecule CD35, CD58 and CD59 in the positive CMV group were significantly down-regulated, and the CD35 and CD59 expression in the negative CMV group were considerably down-regulated (all P <0.05). Compared with negative CMV group, the expression levels of CD58 and CD59 in the positive CMV group were significantly down-regulated (both P < 0.05). The expression levels of CD35 and CD59 in the high active infection subgroup were significantly lower than those in the low active infection subgroup (both P<0.05). Conclusions The more severe active CMV infection after renal transplantation, the lower expression of erythrocytes surface molecule CD35, CD58 and CD59, hinting that red cell immune dysfunction is probably involved with active CMV infection. [ABSTRACT FROM AUTHOR]

Objective To establish the method of cytometric bead assay in combined detection of cystatin C (Cys C) and neutrophil gelatinase-associated lipocalin (NGAL) and to preliminarily investigate the changes in the serum levels of Cys C and NGAL in cytomegalovirus (CMV)-infected patients after renal transplantation. Methods The experimental method was established as following. The effect and stability of microsphere capsulation were evaluated. The detection range of Cys C and NGAL concentration was improved. The concentrations of biotinylated antibody and PE-streptavidin were quantitatively measured. The correlation coefficient of the standard curve was calculated. The serum levels of Cys C and NGAL in the control (n = 10, healthy person), negative CMV (n = 10, CMV-pp65 negative patients after renal transplantation) and positive CMV groups (n = 20, CMV-pp65 positive patients after renal transplantation) were detected using this method. Results The method of cytometric bead assay in combined detection of Cys C and NGAL was established successfully. In the control, negative and positive CMV groups, the serum levels of NGAL were 8 759 (1 465) pg/ml, 10 472 (856) pg / ml and 12 817 (4 533) pg/ml with statistical significance (all P < 0. 001). No statistical significance was noted in terms of Cys C concentration among three groups (all P > 0. 05). Conclusions Combined application of cytometricbead assay detects the standard curve of Cys C and NGAL with high relevance and reproducibility. Preliminary results demonstrates that NGAL concentration is significantly elevated in CMV-infected patients following renal transplantation,hinting that CMV active infection probably leads to acute renal tubular injury. [ABSTRACT FROM AUTHOR]

Objective To observe the changes of serum homocysteine (Hcy) level of renal transplant recipients before and after renal transplantation, and assess the correlation between serum Hcy level and graft function. Methods Thirty-three recipients were included into the transplantation group, who underwent renal allograft transplantation in the Organ Transplant Institute of the 309th Hospital of People's Liberation Army and had renal function recovered stably from January 2013 to June 2014. And 65 patients who were confirmed as chronic renal failure (CRF) by clinical examinations were included into the CRF group and 30 healthy people were included into the control group. A retrospective cross-sectional study was conducted on all of these subjects. Serum Hcy, serum creatinine (Scr) and blood urea nitrogen (BUN) levels of these three groups were compared. Serum Hcy and Scr levels of the transplantation group were continuously monitored before transplantation and at 3, 7, 14 and 21d after transplantation. The correlation between the changes of serum Hcy levels and the renal function before and after transplantation was assessed. Results Serum Hcy level of the CRF group was (25 ±10) μmol/L, which was significantly higher than (9 ±4) μmol/L of the control group and (15 ±9) μmol/L of the transplantation group in stable period,with statistical significance (all in P <0.001). Serum Hcy level of the transplantation group was significantly higher than that of the control group (P <0.001). Scr level of the CRF group,the transplantation group and the control group was (708 ±302) μmol/L, (98 ±23) μmol/L and (72 ±18) μmol/L, respectively. Scr level of the CRF group was significantly higher than those of the transplantation group and the control group (all in P <0.001). BUN level of the CRF group, the transplantation group and the control group was (18.1 ±5.9) mmol/L, (10.9 ±5.3 )mmol/L and (4.9 ± 1.3) mmol/L, respectively. BUN level of the CRF group was significantly higher than that of the transplantation group and the control group (all in P <0.001), and BUN level of the transplantation group was significantly higher than that of the control group (P <0.001). With the improvement in renal function after transplantation, Scr and serum Hcy levels of the transplantation group deceased gradually. At 14 d after transplantation, Hcy level decreased to the minimum of (15 ±5) μmol/L. Compared with (25 ±10) μmol/L before transplantation, the difference had statistical significance (P <0.05). Within 14 d after transplantation, serum Hcy level of the transplantation group was positively correlated with Scr level (r =0.761, P <0.05). Conclusions Serum Hcy level of the renal transplant recipients is correlated with the graft function. The combined detection of serum Hcy and renal function index has certain guiding significance in the prevention of hyperhomocysteinemia and the early assessment of graft function. [ABSTRACT FROM AUTHOR]

预存供体特异性抗体（DSA）阳性是肾移植的禁忌证。预存DSA 增加排斥反应发生率，使致敏患 者存活率降低，直接影响肾移植结果。本文总结1 例预存DSA 强阳性患者，在二次肾移植前采用血浆置换、利 妥昔单抗输注、持续抗胸腺细胞球蛋白滴注，并大剂量静脉注射免疫球蛋白等联合用药方案，抗HLA Ⅱ类抗体 滴度显著降低。术后动态监测该受者群体反应性抗体（PRA）和DSA 水平，早期发现排斥反应，及时采用地塞 米松冲击、抗胸腺细胞球蛋白、大剂量静脉注射免疫球蛋白治疗排斥反应，取得良好效果，患者DSA 消失。术 后随访５个月，移植肾功能良好。 [ABSTRACT FROM AUTHOR]