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数据采集技术的进步导致了数据集规模的飞速上涨，由于数据的大规模和高复杂性引起了严重的数据质量问题，数据清洗是数据活动中必要且重要的环节。为了在保证清洗准确率的情况下有效地降低人工标注成本，提出了一种人工参与的迭代式的数据清洗方法（IDCHI）。该方法在检测模块中提出了数据选择优化方法，使分类器在初始阶段就拥有较高的准确度；并进一步提出了待人工标注数据选择方法，有效地降低人工标注的数据量。实验结果表明该方法可有效且高效地清洗错误数据。

为探究芝麻籽粒萌育过程中含氮物质的变化，采用去离子水、0.5%氯化钙溶液、1%氯化钙溶液对芝麻籽粒浸泡30 min，然后在30 ℃下进行萌育处理，测定芝麻籽粒萌育0～72 h 期间粗蛋白质、可溶性蛋白质、游离氨基酸、蛋白质水解度以及蛋白质分子质量的变化。结果表明：萌育过程中，粗蛋白质含量先减少后增加再减少；可溶性蛋白质含量在0～4 h略微增加，随后下降，72 h时降低了约25%，3种浸种处理方式变化趋势一致；游离氨基酸含量和蛋白质水解度逐渐增大，3种浸种处理方式游离氨基酸含量有显著性差异；SDS-PAGE结果显示，50～95 kDa蛋白质亚基的含量减少，11S蛋白的含量呈波动性变化，最终呈增加趋势，17 kDa蛋白质亚基的含量增加，2S蛋白的含量明显减少。研究说明萌育可以显著改变芝麻籽粒中含氮物质的含量，大分子蛋白质降解为小分子蛋白质，同时2S等小分子蛋白质也发生了降解。 In order to explore the changes of nitrogen-containing substances during sesame seed germination, sesame seeds were soaked respectively in deionized water, 0.5% calcium chloride solution and 1% calcium chloride solution for 30 min and then germinated at 30 ℃. The changes of crude protein, soluble protein, free amino acids, degree of hydrolysis of protein and molecular weight of protein of sesame seed were measured during 0-72 h germination. The results showed that the content of crude protein decreased first, then increased and then decreased. The soluble protein content increased slightly at 0-4 h, then decreased, and decreased by about 25% at 72 h, and variation trend of three soaking methods was consistent.The content of free amino acids and the degree of hydrolysis of protein increased gradually, and there was significant difference in the content of free amino acids among the three soaking treatments. The results of SDS-PAGE showed that the content of 50-95 kDa protein subunit decreased, the content of 11S protein fluctuated during germination and finally increased, the content of 17 kDa protein subunit increased, and the content of 2S protein decreased significantly. The germination can significantly change the nitrogen content in sesame seeds, macromolecular proteins are degraded into small molecular proteins, and small molecular proteins such as 2S are also degraded.

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Objective　To explore the short-term efficacy，safety，and prognostic factors affecting the overall survival （OS） of patients with recurrent high-grade glioma （HGG） treated with bevacizumab （BEV） alone and bevacizumab in combination with temozolomide （TMZ）. Methods　According to the response assessment in neurooncology （RANO） criteria for the treatment of gliomas, objective response rate （ORR） and disease control rate （DCR） were used as indicators to retrospectively analyze the clinical medical records of 31 patients with recurrent HGG at Yunnan Cancer Hospital from August 2020 to July 2023. The patients were divided into a control group （BEV） and a treatment group （BEV+TMZ）, and the recent efficacy of the two groups was evaluated. Cox univariate and multivariate analysis methods were used to analyze the impact of group （ control group vs. treatment group）， gender, age, disease grading, admission score, histopathological classification, etc., on the overall survival （OS） of 31 patients with recurrent HGG. Adverse reactions （ADR） between the two groups were compared using a chi-square test to evaluate the safety. Results　The ORR of the treatment group was 63.16%, and the ORR of the control group was 16.67%. The difference was statistically significant （χ² = 6.419, P = 0.011）. The DCR of the treatment group was 89.47%，and the DCR of the control group was 75.00%. There was no significant difference in DCR （χ² = 0.320， P = 0.571）. The median survival time （mOS） in the control group was 7 months （95%CI: 3.605～10.395），and the median survival time （mOS） in the treatment group was 14 months （95%CI: 3.853～ 24.147）. There was no significant difference in mOS between the two groups （χ² = 0.829，P = 0.363）. There were significant differences in Cox univariate analysis admission score and histopathological classification among 31 patients with recurrent HGG （P < 0.05），and statistically significant differences in histopathological classification by multivariate analysis （P < 0.05）. There was no significant difference in nausea, vomiting, fatigue and diarrhea between the two groups after treatment （P > 0.05）. There were statistically significant differences in the reduction rates of platelets and white blood cells （P < 0.05），after symptomatic treatment, both groups of ADRs were alleviated. Conclusion　The short-term curative effect of the treatment group was better than that of the control group in the treatment of recurrent HGG，the ADRs after recurrent HGG treatment in 2 groups were tolerable and safe, and the histopathological classification may be a prognostic factor affecting the OS of 31 patients with recurrent HGG. [ABSTRACT FROM AUTHOR]

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Objective To clone the gene of Marmota himalayana type Ⅰ interferon receptor β subunit (mhIFNAR2) ， and to perform antibody preparation and functional identification. Methods RT-PCR was used for amplification in the spleen tissue of Marmota himalayana to obtain the sequence， which was cloned to the prokaryotic expression vector pRSET-B to express the recombinant protein. Electrophoresis and Western blot were used for identification. BALB/c mice were immunized with the recombinant protein to prepare the polyclonal antibody of its extracellular domain； immunohistochemistry， immunofluorescence assay， and Western Blot were used for identification， and the method of siRNA blockade was used to investigate its function. An analysis of variance was used for comparison of continuous data between multiple groups， and the least significant difference t-test was used for comparison between two groups. Results A fragment of mhIFNAR2 (149—1 300 bp) was obtained from spleen tissue， which showed the highest homology of 98.05% in marmot. A prokaryotic expression plasmid was successfully constructed for expression of the extracellular domain of the mhIFNAR2 (50-181aa) and was named pRSET-B.mhIFNAR2， and the recombinant protein expressed by this plasmid had a molecular weight of 27 kD， a purity of about 95% after purification， and a concentration of 160 µg/mL. After BALB/c mice were immunized with the purified recombinant protein，1∶1 000 specific polyclonal antibodies were obtained， and immunohistochemistry and immunofluorescence assay showed the expression in cell membrane and cytoplasm. Among the three siRNAs synthesized， the siRNA starting from the 277 locus (siRNA277) could silence the expression of target genes and weaken the interferon signaling pathway compared with the blank control group and the negative control group (both P<0.05) . Conclusion The fragment of mhIFNAR2 is obtained， and the polyclonal antibody for the extracellular domain of mhIFNAR2 is successfully prepared， with relatively high titer and specificity， and can be used for immunohistochemistry， immunofluorescence assay， and Western blot. [ABSTRACT FROM AUTHOR]

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