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The whole cotton stalks are extremely difficult to pull out in Xinjiang in western China. Taking the cotton stalk as the research object, a stalk uprooting equipment with the toothed plate was designed and manufactured, in order to reduce the rate of breakage and leakage, a better performance was achieved without entering into the soil to take out the whole stalks plant, to reduce the influence of the working part on the recycling of the membrane. The device consisted of the V-shaped toothed plate group, the support to harvest clamping belt and tension adjustment. Mathematical analysis was performed on the movement of the V-type tooth plate group, and the support force of the harvesting belt clamping cotton stalks. The clamping point of cotton stalks and supporting stalks trajectory were established in the speed equation of the V-type tooth plate group, according to the agronomic requirements in the mechanical harvesting of cotton planting. The rotating speed of the tooth plate was greater than the proceeding speed. The speed of the clamping belt was much greater than the forward speed of the machine tool level. In order to determine the range of the working parameters, a three-factor and three-level second regression quadratic test was also designed to explore the importance of each parameter in the removal of cotton stalks using Design Expert 13 software. The forward speed, toothed-plate rotational speed, and belt speed were taken as the test factors, with the cotton stalk pulling rate and the leakage rate as the evaluation indexes. The results showed that there was the most significant effect on the pulling-off rate and the leakage rate, when the belt and forward speeds were more than 0.85, and 0.8 m/s, respectively. The response surface test was carried out to obtain the best combination of operating parameters in the cotton stalk pulling equipment, where the forward speed of 0.63 m/s, the rotational speed of the toothed plate of 58.57 r/min, and the belt speed of 0.71 m/s, the pulling breakage rate and the leakage rate were 3.90% and 4.34%, respectively. The field test showed that there was the most significant effect on the pulling breakage rate and the leakage rate, when the forward speed of 0.65 m/s, the rotational speed of the toothed plate of 58 r/min, and the belt speed of 0.71 m/s, the pulling breakage rate and leakage rate of straw pulling were 3.87%, and 4.48%, respectively, the relative error was within 5% with the theoretical prediction, indicating reliable optimization. The finding can also provide a strong reference to design the whole plant pulling device. [ABSTRACT FROM AUTHOR]

he current academic researches on Guizhou’s edible fungi mainly are based on biology, economics, horticulture, and agricultural sciences, etc., which lack historical studies. Therefore, based on the existing local chronicles since the Ming and Qing dynasties, combined with other relevant historical records, this study analyzes and investigates the historical consumption and commercialization of edible fungi in Guizhou, aiming to enrich and supplement the historical documentation related to the economic development history of Guizhou during that period. [ABSTRACT FROM AUTHOR]

Objective　 To explore the anti-inflammatory effect and mechanism of the ethyl acetate extracted fraction of baliospermum solanifolium (bsea) in vitro and in vivo. Methods　 Forty-eight female mice were randomly divided into the normal group, model group, positive control group, and the high-, medium-, and low-dose BSEA groups, respectively. Mice in the positive control group were treated with aspirin (400 mg/kg) by gavage. BSEA (176, 88, and 44 mg/kg, respectively) was given to mice in the high-, medium-, and low-dose groups, and equal volume of 0. 5% sodium carboxymethyl cellulose was given to the mice in the normal group by gavage. All of the above administration groups were given relevant medicine once a day, for consecutive 5 d. Except for the normal group, the acute inflammatory models were established. The carrageenan-induced paw edema in mice was used to explore the anti-inflammatory effects of the BSEA, and the corresponding activity was evaluated via the degree of the foot swelling and the inhibition rate. Chemical constituents were isolated and purified by chromatographic techniques, such as silica gel, MCI, Sephadex LH-20, and semipreparative HPLC, and their structures were identified by analysis of spectral data； Compound 2 was tested for the inhibitory activity against nitric oxide( NO) production in lipopolysaccharide( LPS)-stimulated RAW264. 7 cells and the mechanism of action was investigated by the molecular docking technology. Results　 After intragastrical administration for consecutive 5 d, no anomaly was observed for the mice, indicating that the tested sample had no effect on the normal vital activity of mice in the corresponding dosage conditions. The swelling degree of feet in the positive control group, and the high- and mediumdose BSEA groups was lower than that in the model group (P˂0. 05). Compared with the positive control group, the swelling degree of feet in the medium- and low-dose BSEA groups was higher (P˂0. 05). There were no significant differences in the swelling degree of feet between the positive control group and the high-dose BSEA group (P>0. 05). The swelling inhibition rates of the positive control group, and the high-, medium-, and low-dose BSEA groups were 45. 56%, 35. 57%, 27. 24% and 14. 19%, respectively. Four compounds were isolated and identified as 2,3-trans-6,7,4′-trimethoxyflavan- 3-ol (1), α-linolenic acid (2), methyl linolenate (3), and 1-linolenoyl glycerol (4). Compound 2 exhibited moderate inhibitory activity against LPS-induced NO production with half-maximal inhibitory concentration (IC50) value of (22. 79±1. 48) μmol/L, and had good affinity to five targets relating to inflammation, inducible nitric oxide synthase (iNOS), peroxisome proliferator-activated receptor α (PPARα), nuclear factor-κB (NF-κB), interleukin-1β (IL-1β), and tumour necrosis factor-α( TNF-α), with the docking binding energy of -7. 3, -6. 7, -6. 4, -6. 2, and -5. 9 kCal/mol, respectively. Conclusion　 BSEA exhibits anti-inflammatory activity； Compounds 1-4 are isolated from Baliospermum solanifolium for the first time； Compound 2 exhibits potential anti-inflammatory activity, and it may exert the effect by acting on inflammation-related targets such as iNOS, PPARα, NF-κB, IL-1β, and TNF-α. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To systematically review the efficacy and safety of conventional treatment combined with Suhuang Zhike capsule for acute exacerbation of chronic obstructive pulmonary disease (AECOPD). METHODS: Retrieved from PubMed, the Cochrane Library, Embase, Web of Science, CNKI, CBM, Wanfang Data, VIP database, etc., randomized controlled trials about conventional treatment combined with Suhuang Zhike capsule for AECOPD were collected, and the references of included literature were manually searched for supplementary retrieval from the inception to Jun. 2021. After literature screening, quality evaluation and data extraction of included studies, Meta-analysis was carried out by using RevMan 5. 3 software, and Stata 15. 1 software was used to evaluate publication bias. RESULTS: A total of 1 680 patients with AECOPD were enrolled in 17 studies. Meta-analysis results showed that conventional treatment combined with Suhuang Zhike capsule was better than conventional treatment in improving total response rate (RR = 1. 17,95%CI = 1. 12-1. 22,P<0. 000 01), forced expiratory volume at 1 second (FEV1) (MD =0. 38,95%CI =0. 26-0. 49,P<0. 000 01), the percentage of forced expiratory volume at 1 second in forced vital capacity (FEV1 / FVC) (MD = 11. 44,95%CI = 7. 67-15. 22,P <0. 000 01), and C-reactive protein (CRP) level (SMD =-0. 97,95%CI =-1. 36--0. 59,P<0. 000 01),with statistically significant difference; there was no statistical significance in the incidence of adverse drug reaction between two groups (RR =1. 33,95%CI =0. 85-2. 08,P =0. 21). Results of publication bias test and non-parametric trim and fill method showed bias in the outcome indexes of total effective rate, but the possible publication bias did not affect the results. CONCLUSIONS: Based on the existing evidence, Suhuang Zhike capsule has positive efficacy in the adjuvant treatment of AECOPD, can improve the levels of lung function indicators (FEV1, FEV1 / FVC) and reduce the level of inflammation index (CRP), and shows good safety, but the above results still need to be reviewed with caution. [ABSTRACT FROM AUTHOR]

Peritoneal metastases (PM) are defined as the primary or secondary occurrence/progression of malignant tumor in peritoneum. PM were previously thought to be a terminal disease without effective treatment, with short survival and poor prognosis. With the change in the understanding of PM, the oncology communities regard it as a curable regional cancer metastasis, and create a comprehensive treatment technology system with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy as the core, and establish professional PM treatment centers based on this. The professional PM treatment centers have significantly prolonged the survival of patients, and some patients can even achieve clinical cure. However, in China, there are very few professional PM treatment centers, but the number of PM patients is huge, and most of the patients can’t receive professional treatment, resulting in poor survival and prognosis. Based on the cancer statistics in 2015 published by China National Cancer Center Registry and clinical outcome literature on peritoneal metastasis, this paper uses clinical epidemiology methodology to calculate the number of newly diagnosed patients with peritoneal metastasis, to estimate the number of specialized peritoneal cancer centers required, to provide data support for the promotion of professional treatment technology system for PM in our country, and to boost the development of peritoneal oncology. [ABSTRACT FROM AUTHOR]

With the continuous consumption of non-renewable resources, renewable biomass carbon material resources have attracted the researchers' attentions. In this paper, the easily available agaric was selected as the carbon source, to prepare biomass carbon materials by a high-temperature heat treatment. The effects of different heat treatment temperatures on the micromorphology and microwave absorption properties of materials were studied. Finally, the Structure performance relationship between micromorphology and absorption properties of materials was established. The conclusions can be summarized as follows: In agaric-derived biomass carbon materials, a large number of nanorods are intertwined, showing a jungle shape. The interior is rich in mesopores, which is due to the fact that the bound water and other trace elements contained in the material are removed by thermal evaporation during the carbonization process of high temperature heat treatment. The microwave absorption per-formance of the sample changes with the change of heat treatment temperature. When the heat treatment temperature is 700 °C and the thickness is 4.98 mm, the sample reaches the lowest reflection loss value of 41. 14 dB at the 11.33 GHz, and the effective absorption bandwidth is 0.75 GHz, which has the best electromagnetic wave absorption performance. [ABSTRACT FROM AUTHOR]

In order to solve the problems of frequency splitting and low efficiency in wireless power transfer system, the electrical-field coupled wireless power transfer system with double-side LCLC compensation topology is taken as the research object in this study. The effect laws of variable parameters on the system output are analyzed by system modeling. The relationship between system efficiency and the important parameters is derived using the circuit theory, and the system efficiency is analyzed in detail using MATLAB simulation software. The correctness of simulation results are verified by experiments. The results show that adjusting the capacitance value of the coupling mechanism below the frequency division point can avoid frequency splitting in the system, while fixing the relative position of the coupling plates can improve the system efficiency by increasing the frequency of the system to the critical point of efficiency, and the best efficiency of the system can reach 89.4%. [ABSTRACT FROM AUTHOR]

BACKGROUND: The assessment of fracture risk still depends on bone mineral density testing. However, it ignores other mechanisms that affect fractures, including three-dimensional bone structures and material properties on multiple scales. Although bone mineral density provides useful information, its value in predicting fracture risk is limited. OBJECTIVE: A three-dimensional finite element model of proximal femur with different cancellous bone volume fractions was constructed to explore the effect of bone volume fraction on the apparent mechanical response of proximal femur. METHODS: The CT data of a volunteer’s proximal femur were collected and imported into Mimics to reconstruct the three-dimensional model in DICOM format. The osteoporotic cortical bone and cancellous bone were given corresponding material parameters. Then, the model was imported into Abaqus and the finite element model with 35%, 30%, 25%, 20% and 15% cancellous bone volume fractions was constructed by uniform deletion of the script. A reference point was established above the femoral head and a concentrated load was applied to the area of contact with the acetabulum above the femoral head to analyze the difference of mechanical response of the proximal femur under orthostatic stress. RESULTS AND CONCLUSION: (1) Under the condition of standing load, the tensile stress on the upper and outer side of the femoral neck was always greater than the compressive stress on the medial and inferior side of the neck. With the decrease of cancellous bone volume fraction, the tensile stress and compressive stress of proximal femur increased gradually, and the maximum tensile stress and maximum compressive stress of 15% model were 1.91 times and 1.42 times of 35% model, respectively. The maximum principal strain increased by 4.76 times, and the overall stiffness of the femur decreased by 58%. (2) Under the condition of standing on one foot, the cortical bone of the femur bore more stress than the cancellous bone, and the cancellous bone played an indispensable role in the overall elastic response of the femur. (3) With the decrease of the volume fraction of cancellous bone, the tensile stress of the lateral superior side of the femoral neck increased greatly. The region of the lateral superior side of the neck is the most significant area of bone mass loss, and it is also in the area of stress concentration under the fall load. This suggests whether the relevant parameters of the lateral superior side of the neck (such as bone mineral density and volume fraction) may become a more sensitive index for predicting brittle femoral neck fracture, which is worth further exploring. [ABSTRACT FROM AUTHOR]

Objective To observe the change of SDF-1 levels in the bone and skeletal muscle during the treatment of postmenopausal osteoporosis with TCM nourishing the kidney-yin, warming the kidney-yang, tonifying spleen, and tonifying kidney and spleen, and to explore the modern medical mechanism of TCM in the prevention and treatment of osteoporosis. Methods Except the normal group, ovariectomized rats with osteoporosis were randomly divided into model group, tonifying kidney-yin group, tonifying kidney-yang group, invigorating spleen group, tonifying kidney and invigorating spleen group, and Fosamax group. Serum ALP, TRACP, bone and skeletal muscle SDF-1 levels in rats of each group were examined with ELISA method. Results 1) Compared to the normal group, ALP and TRACP levels in the model group increased significantly (P<0. 01). All treated-groups showed decreased levels of ALP and TRACP comparing to those in the model group (P<0. 01), especially in the Fosamax group, followed by that in the tonifying kidney and invigorating spleen group. 2) SDF-1 levels in the bone and skeletal muscle in the model group were significantly higher than those in the normal group (P<0. 01). Compared to the model group, bone SDF-1 levels in the tonifying kidney-yang group and the Fosamax group decreased significantly (P<0. 01), followed by that in the invigorating spleen group and tonifying kidney-yin group (P<0. 05). Skeletal muscle SDF-1 levels in the Fosamax group and invigorating spleen group were significantly lower than those in the model group (P<0. 01). Conclusion I) The occurrence of osteoporosis is related to the increase of SDF-1 expression in the bone and skeletal muscle. 2) Treatment with nourishing the kidney-yin, warming the kidneyyang, invigorating spleen and tonifying kidney and spleen show effects on postmenopausal osteoporosis by down-regulating SDF-1 expressions in the bone and skeletal muscle. [ABSTRACT FROM AUTHOR]

Aiming at the problems of the existing Byzantine fault-tolerant consensus mechanism applied to alliance chain, such as insufficient scalability and difficulty to support efficient and secure consensus under large-scale network nodes, this paper proposed a practical Byzantine fault-tolerant consensus algorithm(called H-PBFT) based on Raft clustering grouping by node grouping strategy. Firstly, the shuffled frog leaping algorithm combined with K-medoids clustering strategy using to cluster nodes in the system into multiple groups participated. The central nodes of each cluster nodes in the PBFT consensus of the main consensus cluster and the Raft algorithm introduced the improved supervision node was used to achieve consensus in each cluster group. Intra-group consensus mechanism supervised the introduction of nodes to make Raft algorithm Byzantine-resistant. Experimental results show that, compared with PBFT and Raft, this algorithm can improve fault tolerance performance in large-scale network node environment, and can also cluster nodes quickly and efficiently, improve consensus efficiency, reduce consensus communication overhead and complexity. This algorithm has better scalability, which can be better applied in alliance chain scenarios. [ABSTRACT FROM AUTHOR]

This paper proposed a credit-score-based Byzantine fault-tolerant consensus algorithm, called CS-Raft, to address the inadequate performance of the current consensus algorithms in consortium chains. Initially, it assigned credit score attributes to all nodes and updated the credit score of each node according to their consensus behavior, activity level, time of joining the cluster and other metrics. The higher the credit score, the higher the node's trustworthiness. Secondly, it selected monitoring nodes based on their credit scores, which had the authority to inspect and participate in leader elections. The setting of monitoring nodes could effectively resist attacks from Byzantine malicious nodes. Finally, it made improvements to address the issue of vote splitting in leader elections, thereby enhancing the speed of leader election. Experimental analysis shows that compared to the PBFT algorithm, the CS-Raft algorithm effectively reduces consensus latency, increases system throughput, and accelerates leader election speed while achieving Byzantine fault tolerance. [ABSTRACT FROM AUTHOR]

Objective To study the expressions and significance of chemokine CCL2 in glioma cell lines， and to screen out Glioma cell lines with high expression of CCL2. Methods The expression levels of CCL2 in five glioma cell lines including T98G， U251， SHG44， U373 and U87 were detected by Western blot. Results CCL2 was expressed in all 5 glioma cell lines， and the expression levels of CCL2 in U251 and U373 were higher than those in T98G， SHG44 and U87 cell lines（P < 0.001） . Conclusion CCL2 is expressed in glioma cell lines T98G， U251， SHG44， U373 and U87，suggesting that CCL2 may play an important role in the occurrence and development of glioma. CCL2 is highly expressed in U251 and U373， laying a foundation for further studies on the role of CCL2 in glioma. [ABSTRACT FROM AUTHOR]

It analyzes common ventilation apparatuses in university labs from their features and control modes, proposes two conceptions, continuous ventilation volume and temporary ventilation volume, and calculates the ventilation volume of each apparatus accordingly, then completes these for the room. The total ventilation volume of air is based on their sum, and is used to choose an exhaust fan, where one is continuous volume, the other is temporary volume multiplied by the coefficient of simultaneous using ratio. The noise control and the exhaust air purification also should be considered.The makeup air of each room is calculated by the apparatuses in it, and controlled by the difference of ventilation volume and makeup air volume. The makeup air is feed by built-in air tubes, or distributed air source heat pump. The system of the whole building is controlled by three layers, i. e., apparatuses, rooms, building. Good quality of installation and adjustment are also needed. [ABSTRACT FROM AUTHOR]

Objective: To investigate the roles of Bakuchiol (Bak) on HepG2 cell and the effects of co-treatment of Bak and Tumor necrosis factor- related apoptosis-inducing ligand (TRAIL) on HepG2 cells, as well as the inner mechanisms. Methods: Cells were treated with different concentrations of Bak or Bak+TRAILand cell vitality was detected 24 h later. After Bak treatment, cell oxidative stress levels were examined through DCF-DA staining and ROS, GSH detection. Western blot analysis was conducted to detected the expressions of Death receptor 4 (DR4) and DR5. After Bak+TRAIL treatment, cell apoptosis status was detected. Furthermore, ROS scavenger NAC was used to inhibit oxidative stress and DR4, DR5, Bax expressions were detected. Results: Both Bak and TRAIL can reduce HepG2 cell vitality. Co-treatment of Bak and TRAIL further reduced the cell vitality. Bak treatment increased ROS production, DR expressions and reduced cellular GSH level. Co-treatment of Bak and TRAIL induced higher level of apoptosis compared with single-drug treatment. Compared with the Bak + TRAIL group, NAC treatment reduced ROS level and reduced DR expression, as well as the Bak+TRAIL induced apoptosis levels. Conclusion: Bak enhances TRIAL induced HepG2 cell apoptosis and tumor growth via activating cellular oxidative stress and up regulating DR4 and DR5 expressions. [ABSTRACT FROM AUTHOR]

This paper studied the core model required for testing feasibility of high-speed train operation plan rapidly by the means of simulation. With the help of improved Petri nets, it regarded the train as token, and regarded line and station as place respectively in accordance with the high-speed train operation characteristics and network function. It realized location transformation and running information transfer of high-speed train through customizing a variety of transition. It built the model based on the thought of component combination, considering the random disturbance in the process of train running, simulation framework could be generated quickly and the system operation could be completed according to the different test requirements and the needed network data, but this model was only applicable to the single-direction running transport management mode usually adopted in high-speed railway. Simulation example shows that model can be developed, the simulation results basically are coincide with the objective reality, and it not only can test the feasibility of the high-speed train operation plan, also can be used as a support model to develop the simulation platform with more purpose. [ABSTRACT FROM AUTHOR]

For solving the trade-off problem between energy consumption and performance during resource allocation in cloud data center, this paper designed an energy-efficient optimizaiton strategy combined DVFS-aware and dynamic virtual machine consolidation. First, through a new DVFS-performance algorithm, this strategy could reduce power consumption while preventing performance degradation. Then, it desinged a DVFS-aware consolidation algorithm to optimize power consumption, considering the DVFS configuration that would be necessary when mapping virtual machines to maintain QoS. Finally, it performed an extensive evaluation on the simulation toolkit using real cloud traces. Experimental results show that this strategy can obtain less virtual machines migration and provide substantial energy savings of data center for scenarios under dynamic in workload conditions without reducing QoS and increasing SLA violation, which can get better trade-off between energy consumption and performance. [ABSTRACT FROM AUTHOR]

Due to the strong privacy of industrial Internet of Things( IloT) data, safely storing the data has become a key issue in the development of IloT. This paper proposed a model of IloT data storage based on consortium blockchain to solve this problem. In order to ensure the efficiency and security of data storage, firstly, the method built a node status credit evaluation model based on the original PBFT algorithm and added a voting mechanism. Secondly, combined with node status, it set multiple key generation centers in SM9 digital signature algorithm to jointly participate in key generation. This paper experimentally analyzed the throughput, consensus delay and communication overhead of the model. The experimental results show that compared with the traditional model, the proposed model further guarantees the security in terms of data storage, reducing hacker attacks and preventing data leakage or tampering, and increases the communication overhead by 40%. [ABSTRACT FROM AUTHOR]

A sort of Polycarboxyl/methylsilsesquioxane (PCMSQ) nano-hybrid emulsions were prepared Via hydrolytic condensation method of methyltriethoxysilane (MTES) and carboxy silane (CTES) which was first fabricated by 7-aminopropyltriethoxysilane (KH550) with maleic anhydride (MA) ,and sodium hydroxyl as the catalyst,primary alcobol ethoxylate (AEO3/AEO9) as mixed emulsifiers. Effects of the emulsifier doses,the catalyst concentration and the oil/water ratio were discussed. Structure,particle size, micromorphology and thermal stability of PCMSQ nanospheres were investigated by FTIR, XPS,DLS, SEM and TGA. Results indicated that structure of the PCMSQ was consistent with the expectant outcome and thermal stability of the PCMSQ nanospheres was excellent. When 휔)AEO3/AEO9) is 0.36%~0.54% ,NaOH concentration is 5~50mmol·L-1 and oil/water ratio is 1:10~1:12,average particle sizes of the PCMSQ nanospheres were ranged of 100~200 nm. Meanwhile these PCMSQ nanospheres with the uniform average diameter and monodisperse, which were regularly spheres. [ABSTRACT FROM AUTHOR]

Objective : To explore the establishment of professional pharmaceutical care mode for children with epilepsy in the inpatient and outpatient departments. Methods : In the process of implementing professional pharmaceutical care mode,the status of drug treatment and chief concerns of family dependants were summarized. Results : Since the establishment of anti-epileptic treatment service from June 2016 to the end of February 2017,the total number of medical care given to epileptic children in the epileptic departments of the hospital amounted to 240 person times,and pharmaceutical care was delivered to 50 cases. Face-to-face communication revealed that 60%-70% of the dependants did not have a comprehensive understanding of disease monitoring, drug administration, chief concerns of epileptic treatment and monitoring of adverse drug reactions (ADRs). About 5% of the dependants were totally ignorant of the knowledge in epilepsy,and about 25% of the dependants were too excessively worried about ADRs. The percentage of the dependants who asked for drug switch or drug withdrawal was as high as 26%. Conclusion : A considerable part of family dependants knew little about epilepsy drug treatment and monitoring of self - medication safety. Professional pharmaceutical care under the comprehensive and tight control of the inpatient and outpatient departments was of positive significance for family dependants to have a better understanding in the effects of epilepsy drug treatment and elimination of excessive concerns over ADRs of prolonged medication,thus promo ting medication compliance. [ABSTRACT FROM AUTHOR]

[Objective] To investigate the regulatory role of basic leucine zipper (bZIP) transcription factors in anthocyanin metabolism in ‘Hongmantang’ apple. [Method] We cloned a bZIP gene from ‘Hongmantang’ red-flesh apple, analyzed it and its sequence characteristics, studied the subcellular localization of its encoded protein using onion epidermis cell transient overexpression, and investigated its expression pattern in ‘Hongmantang’ red-flesh apple fruits at different ripening stages using quantitative real time PCR（RT-qPCR）. Furthermore, we explored its regulatory roles in the expressions of genes related to anthocyanin accumulation and biosynthesis based on the transient overexpression experiments in tobacco leaves, apple leaves and fruits. [Result] This bZIP gene was intron-less and shared the highest similarity with MdbZIP43 (XP_008393381.1), thus was named as MbbZIP43. Its coding sequence was 522 bp, which encoded a 173 aa hydrophilic nucleus-localized protein with conserved bZIP_plant_GBF1 domain. RT-qPCR analysis revealed that MbbZIP43 showed a ‘rise-fall’ expression change pattern in ‘Hongmantang’ fruits during ripening, with the highest expression in the fruits at 11 weeks post flowering. Correlation analysis results showed that MbbZIP43 expression was positively correlated with the total flavonoids and anthocyanin contents in ‘Hongmantang’ fruits, indicating that it might play regulatory roles in flavonoid biosynthesis. Consistently, transient overexpression of MbbZIP43 improved significantly the anthocyanin accumulations by 17.42%, 25.66% and 48.99% in tobacco leaves, apple leaves and apple fruits, respectively. Moreover, RT-qPCR analysis results showed that, compared to the empty vector control, the expressions of anthocyanin biosynthesis related structural genes（CHI, F3'H, DFR and UFGT）in the apple leaves overexpressing MbbZIP43 were up-regulated by 2.27-, 1.84-, 2.39- and 2.89-fold, respectively. And their expressions in the peels of apple fruits overexpressing MbbZIP43 were up-regulated by 1.79-, 1.70-, 1.35- and 1.51-fold, respectively. These results indicated that the gene's transient overexpression may improve anthocyanin accumulation by activating the expressions of the anthocyanin biosynthesis related structural genes. [Conclusion] MbbZIP43 can promote anthocyanins accumulation by activating the expression of anthocyanin biosynthesis related structural genes, including CHI, UFGT and so on. [ABSTRACT FROM AUTHOR]

In order to investigate the lethal dose of herbicide and its effect on physiological and biochemical characteristics of foxtail millet, in this study, three-leaf stage Jigu25 seedlings were treated with different concentrations of imazethapyr in seedling tray culture. The lethal dose of imazethapyr on Jigu25 seedlings was screened under different treatment concentrations and time. At the same time, the changes of chlorophyll content, electrolyte permeability, malondialdehyde(MDA) content, peroxidase(POD), superoxide dismutase(SOD) and catalase(CAT) activities of foxtail millet seedlings after imazethapyr treatment were measured. The results showed that the lethal dose of imazethapyr on Jigu25 seedlings was 3 375 mL/ha, and it was found that with the increase of herbicide concentration(2 250, 3 375, and 4 500 mL/ha) and the extension of treatment time, the corresponding physiological indexes of chlorophyll content, electrolyte permeability, MDA content, POD, SOD and CAT activities of Jigu25 seedlings showed significant changes at different time compared with the control. This study showed that after spraying a lethal dose of 3 375 mL/ha of imazethapyr, foxtail millet seedlings were subjected to stress and physiological protection mechanism was activated. [ABSTRACT FROM AUTHOR]

Objective: In order to provide evidence for the meat quality study, conservation and breeding, feeding and management, and clinical treatment on adult Sichuan white rabbit, and to investigate the germplasm characteristics of Sichuan white rabbit in terms of blood physiochemical properties. Methods: Twenty healthy about 1.5~3 kg adult Sichuan white rabbits, male and female in half, were bred in conventional environment, and fasting for 12 h before experiment, drinking water with freedom, then took blood in the morning with empty stomach. The blood physiological and biochemical indicators were determined using automatic blood analyzer and automatic biochemical analyzer, and were compared between female and male rabbits. Results: Among the blood physiological indicators, only platelets presented significant difference between males and females (P＜0.05), and none of the other indicators presented significant differences (P＞0.05); among the biochemical indicators, ALP, total bilirubin and creatinine presented differences between female and male Sichuan white rabbits (P＜0.05), and none of the other indicators presented significant differences (P＞0.05). Conclusion: Most of the blood physiological and biochemical indicators measured presented no significant differences between the female and male adult Sichuan white rabbits, indicating that most of the physiological and biochemical indicators are comparable between female and male adult Sichuan white rabbits without being subject to the influence of gender. [ABSTRACT FROM AUTHOR]

Objective: In order to provide evidence for the meat quality study, conservation and breeding, feeding and management, and clinical treatment on adult Sichuan white rabbit, and to investigate the germplasm characteristics of Sichuan white rabbit in terms of blood physiochemical properties. Methods: Twenty healthy about 1.5~3 kg adult Sichuan white rabbits, male and female in half, were bred in conventional environment, and fasting for 12 h before experiment, drinking water with freedom, then took blood in the morning with empty stomach. The blood physiological and biochemical indicators were determined using automatic blood analyzer and automatic biochemical analyzer, and were compared between female and male rabbits. Results: Among the blood physiological indicators, only platelets presented significant difference between males and females (P＜0.05), and none of the other indicators presented significant differences (P＞0.05); among the biochemical indicators, ALP, total bilirubin and creatinine presented differences between female and male Sichuan white rabbits (P＜0.05), and none of the other indicators presented significant differences (P＞0.05). Conclusion: Most of the blood physiological and biochemical indicators measured presented no significant differences between the female and male adult Sichuan white rabbits, indicating that most of the physiological and biochemical indicators are comparable between female and male adult Sichuan white rabbits without being subject to the influence of gender. [ABSTRACT FROM AUTHOR]

Objective: To discuss the effects of fatty acid amide hydrolase inhibitor URB597 on the apoptosis of human hepatoma cell line MHCC97H in vitro and its underlying mechanism. Methods: Different concentrations of URB597 （1, 5 and 10 μmol/L respectively） were administered to incubate MHCC97H. Flow cytometry was used to analyze the effects of URB597 on apoptosis in MHCC97H cells. Western-blot assay was applied to detect the levels of the apoptosis-inducing factors （AIF）, Bcl-2 and Bax with or without URB597. Caspase 3 detection kit was used to determine the activity of caspase 3. Results: （1） Following treatment with URB597 at different concentrations （1, 5 and 10 μmol/L） for 1, 4 and 7 days, the number of apoptotic cells was significantly increased with a time-dependent tendency. （2） After treatment with URB597 at different concentrations for 4 days, the activity of caspase 3 and AIF levels in the nucleus were increased significantly. （3） Following treatment with URB597 at a concentration of 10 μmol/L for 4 days, the expression level of Bcl-2 was obviously down-regulated, while Bax level was obviously up-regulated, when compared with those of the control group, and the Bcl-2/Bax ratio was obviously reduced. PI3K inhibitor LY294002 could also significantly reduce the Bcl-2/Bax ratio. However, as compared with the 10 μmol/L control level, its effect on the Bcl-2/Bax ratio was relatively lower. Conclusion: URB597 could enhance the apoptosis of MHCC97H cells by down-regulating the ratio of Bcl-2/Bax, further inducing caspase-dependent and caspase-independent cell apoptotic pathway, which was partial-dependent on PI3K/Akt signal pathway. [ABSTRACT FROM AUTHOR]

Dry limed cowhide collagen fibers were rewetted, acid-swollen and ground to obtain a homogeneous collagen syrup. Different concentrations of tea polyphenols (0.2 wt% 1.0 wt% and 1 8 wt%) and glycerol (30 wt%, all based on dry collagen fibers) were orderly added into the above collagen syrup and fully dispersed to form the film-forming solutions. Finally a series of collagen composite films (CCF) were prepared by solution casting method. The structure, thermal properties, hydrophilicity, microstructure and mechanical properties of CCF were studied by Fourier transform infrared spectrometer (FT-IR), X-ray diffrotometer ( XRD), differential scanning calorimetry (DSC), water contact angle meter, scanning electron microscope ( SEM) and tensile testing machine. Results showed that the integrity of the three-strand spiral collagen structure was maintained in CCF, but the amorphous structure was dominant. With the increase of the amount of tea polyphenols (TP), the collagen molecular chains in CCF film were mainly distributed in an amorphous form, and the intermolecular lateral packing distance among the peptide chains of triple helix tended to increase, and thermal stability of CCF film increased. Scanning electron microscopy (SEM) results showed that the control film surface without TP was not smooth with some cavities, and the film cross-section was not compact. With the increase of the TP amount, the voids on the CCF surface disappeared and the number of particles gradually increased, and the compactness of the film section also increased. The static water contact angle of the film surface increased from 93.4 ° to 124.3°, indicating the increased hydrophobicity. The tensile strength of the film increased from 5.17 Mpa to 9.78 Mpa, and the elongation decreased from 6.1% to 2.5%. Therefore, this study can bring a new opportunity for the resource utilization of dry limed cowhide collagen fibers. [ABSTRACT FROM AUTHOR]

It is worth studying and exploring the research on the personalized recommendation technique that is used in news reader application to help users fast access interested news with its rapid and accurate features. We design and implement a news recommendation system based on user collaborative filtering recommendation technology. By collecting user data, calculating the reading time factor to correct user preference value, incorporating the influence of the news heat and punishing user similarity value by the heat, and then conducting Top-N ranking for user's unread news based on similar neighbor sets, the news of interest is pushed to the users. The test results of the news recommendation system show that it can provide real-time updates for the user interest model accurately and each function has achieved prospective design aim. [ABSTRACT FROM AUTHOR]

[ Objective] This paper aimed to investigate the effects of heat stress on reproductive performance, serum biochemical indexes and reproductive hormones in female rabbit of Qixing. [ Method ] A total of 200 healthy Qixing females were randomly divided into two groups : The preference temperature group ( 21 ± 1) "C and the heat stress group ( 32 ± 1 ) "C, with 100 rabbits in each treatment. [ Result] ( i) The conception rate, litter size, birth weight and weaning survival rate of the females in heat stress group were lower than those in preference tem perature group, but there was no significant difference between them. ( ii) The serum GOT activity in the heat stress group was significantly higher than that in the preference temperature group from the 21 days of pregnancy to the 0 days of postpartum ( P < 0. 05), the serum GPT activityat the day of mating and the 7 days of pregnancy was significantly higher than that of the preference temperature group(P < 0. 05), but there was no significant difference be tween the two groups at other time points. (iii) The serum cor tisol in the heat stress group was significantly higher than that in the preference temperature group from the 11 days of pregnancy to the 0 days of postpartum ( P < 0. 05), while there was no sig nificant difference between the two groups at other time points. For the serum triiodothyronine ( T3 ) in the heat stress group was significantly lower than that of preference temperature group at 11 days of pregnancy and 18 days of pregnancy ( P < 0. 05), there was no significant difference between them at other time points. The serum immunoglobulin A ( lgA) in heat stress group was signifi cantly lower than that in preference temperature group at 18 days of pregnancy ( P <0. 05), there was no significant difference between them at other time points. However, the serum immunoglobulin G ( lgG) in the heat stress group was lower than that in the preference temperature group at all time points, but there was no significant difference between them. (iv) During the whole experiment, The serum estradiol ( E2) in the heat stress group was lower than that in the preference temperature group, but the difference was not significant. For the serum proges terone ( P4) in the heat stress group was significantly lower than the preference temperature group at the 11 days of pregnancy, the 0 days of postpartum and the 28 days of postpartum ( P < 0. 05), and there was no significant difference between the two groups at other time points. The serum prolactin ( PRL) in heat stress group was significantly lower than that the preference temperature group at thel 1 days of postpar tum and the 18 days of postpartum ( P < 0. 05), but there was no significant difference at other time points. [Conclusion] In conclusion, the female rabbit of Qixing showed better reproductive characteristics under heat stress, which might be related to the changes of serum biochemi cal indexes and reproductive hormones. This results would provide a theoretical basis for breeding new rabbit varieties with the characters of resistance to heat. [ABSTRACT FROM AUTHOR]

Objective To analyze the expressions of non-small-cell lung cancer (NSCLC) driver genes and their mutation distribution characteristics in the Yunnan-Kweichow plateau, and to provide evidences for personalized molecular targeted therapy of lung cancer in high-incidence areas. Methods A retrospective analysis was performed on the medical records of patients with NSCLC who underwent combined lung cancer 8 gene detection, including epidermal growth factor receptor (EGFR), rat sarcoma viral oncogene (RAS), anaplastic lymphoma kinase (ALK), RET proto-oncogene (RET), v-Raf murine sarcoma viral oncogene homolog (BRAF), ROS proto-oncogene 1 (ROS1), human epidermal growth factor receptor-2 (HER-2), and cellular-mesenchymal to epithelial transition factor (MET), from January 2016 to August 2019 in Yunnan Cancer Hospital. Besides, we analyzed the expressions of NSCLC driver genes and their mutation distributions. Results The positive rate of NSCLC driver genes in Yunnan was 67.05%(1 508/2 249). The mutation rates in Xishuangbanna (76.92%), Yuxi (72.38%), Xuanwei (71.88%), Qujing (71.24%), and Honghe (71.79%) were significantly higher than other areas. The mutation rates of Hui (84.38%), Hani (85.00%), Zhuang (75.00%), Buyi (100%), Manchu (100%), Tujia (100%) and Achang (100%) are significantly higher than the minority national average. Driver gene mutations were related to gender (P<0.001), smoking history (P<0.001), age (P<0.001), pathological type (P<0.001), and whether the Xuanwei area (P=0.027), but not related to the nationality (P=0.748) and family history of lung cancer (P=0.676). The mutation rates of EGFR, RAS, BRAF, HER-2 and MET genes were 44.46%, 10.98%, 1.24%, 0.89% and 0.76%, and the rearrangement rates of ALK, RET and ROS1 genes were 4.67%, 1.29% and 0.89%, respectively.The mutation rate of EGFR in females was 56.67%, which was higher than 33.19% in males (P<0.001). The mutation rate of RAS in males was 12.66%, which was higher than 9.17% in females (P=0.010). The mutation rate of RAS in the Han was 11.49%, which was higher than 7.17% in the minority (P=0.032). The rate of RAS mutation in Xuanwei patients was 24.74%, significantly higher than 8.15% in non-Xuanwei area (P<0.001), and the EGFR mutation rate was 40.63%, which was lower than 45.25% in non-Xuanwei area (P=0.045). The rate of ALK rearrangement in Xuanwei patients was 1.56%, which was significantly lower than 5.31% in the non-Xuanwei area (P<0.001), and no HER-2 mutation patients were detected in Xuanwei area. The mutation rate of EGFR in patients with non-smoking history was 51.10%, significantly higher than 29.70% of patients with smoking history (P<0.001). Meanwhile, the rate of ALK rearrangement with non-smoking history patients was 5.35%, which was also higher than 3.16% of patients with smoking history (P<0.001). The rate of RAS mutation in patients with non-smoking history was 9.34%, lower than 14.63% of patients with smoking history (P=0.008). Conclusions The positive rate of driven gene expression in NSCLC patients from the Yunnan-Kweichow Plateau is slightly lower than the national average. The rates of EGFR and RAS mutations are similar to the domestic average. The rates of ROS1, ALK and RET genes rearrangements and the rates of BRAF, HER2 and MET gene mutations are slightly lower than the national average. EGFR, RAS and ALK genes in the NSCLC patients from Yunnan-Kweichow Plateau have high positive rates, and display different demographic and clinical characteristics, which are of great significance in the selection of targeted therapy populations. [ABSTRACT FROM AUTHOR]

Objective To analyze the EGFR mutation profile of lung cancer patients in Yunnan, and to provide evidence for clinical personalized treatment. Methods Demographic and clinical data of 2 967 lung cancer patients undergoing EGFR identification were collected and analyzed from January 2014 to August 2019 in Yunnan Cancer Hospital. Results The proportion of EGFR mutation in 2 967 patients with lung cancer was 46.2%. Univariate analysis showed that the proportion of EGFR mutation in women was higher than that in men (P<0.001) and displayed a downward trend with age (P=0.03). The mutation rate of ethnic minorities was higher than Han (P=0.012). Mutation rate in patients without smoking history was higher than those with smoking history (P<0.001), and patients without drinking history was higher than patients with drinking history (P<0.001). Mutation rate in patients without family history of lung cancer was higher than those with family history (P=0.008). The mutation rate of adenocarcinoma was higher than other pathological types (P<0.001). The mutation rate was different among stages, and it was higher in early patients than that in advanced patients (P<0.001). The mutation rate of tissue specimens was higher than those of cytology and peripheral blood samples (P<0.001). The mutation rate of Xuanwei area was lower than that in non-Xuanwei area (P<0.001). Multivariate analysis showed that gender (P<0.001), age (P=0.036), smoking history (P<0.001), pathological type (P<0.001), specimen type (P<0.001), and whether or not Xuanwei area (P<0.001) were the independent factors of EGFR mutation.The EGFR mutation was more common in female, non-smokers, adenocarcinoma, non-Xuanwei area, tissue specimen and young lung cancer patients.The mutation types of EGFR in 1 370 cases mainly included 19-Del and L858R. The predominant mutation of EGFR in Xuanwei area was L858R, while in non-Xuanwei area was 19-Del.The mutation rates of G719X, G719X+ L861Q, G719X+ S768I, and S768I in Xuanwei were higher while the mutation rates of 19-Del, L858R, and 20-ins were lower than non-Xuanwei area (P<0.05). The 19-Del mutation rate of ethnic minorities is higher than that of Han (P<0.001). The combined mutation rate of G719X, L861Q in Han was higher than that of ethnic minorities (P=0.005). Conclusions The EGFR mutation rate in lung cancer patients in Yunnan is similar to Asian and Chinese, and higher in female, non-smokers, adenocarcinomas, young and non-Xuanwei area patients. The most common types of EGFR mutation in Yunnan are 19-Del and L858R. The predominant mutation of EGFR in Xuanwei area is L858R, while in non-Xuanwei area is 19-Del. The mutation rates of G719X, G719X+ L861Q, G719X+ S768I and S768I are higher in Xuanwei patients than those in non-Xuanwei patients. The combined mutation rate of G719X and L861Q in Han nationality is higher than that of ethnic minorities. [ABSTRACT FROM AUTHOR]

In this paper, we aim to mine the interesting locations and the frequent travel sequences in a given geo-spatial region, by taking into account the users' historic travel experiences as well as the correlation between locations. First, a new partition method is proposed to divide the trajectories into a set of line segments (in which contains the stationary moving sequence), the start and end points of those line segments are collected as characteristic points. Then clustering method is introduced to cluster the geographical-similar endpoints into groups for detecting fixed territories. Finally an abstract path network is generated to show the linkage relations between the mined fixed territories. The proposed method can be used to detect a user's frequent movement paths as well as fixed territories for better personalized geographical service. [ABSTRACT FROM AUTHOR]

Objective To determine the prevalence of sarcopenia according to fracture sites and to evaluate the associated risk factors in female patients with osteoporotic fractures. Methods A total of 112 patients aged 50 years or older with an osteoporotic fracture(hip, spine, or wrist) were enrolled in this study. A diagnosis of sarcopenia was confirmed using whole-body densitometry for skeletal muscle mass measurement. Logistic regression analysis was used to analyze the risk factors for sarcopenia. Results Of 112 female patients treated for osteoporotic fractures between April 2015 and August 2017, sarcopenia was diagnosed in 42 cases(37.5%). Among those, 41.8%(18/43) had hip fractures, 36.7%(15/41) had spine fractures, and 32.1.6%(9/28) had distal radius fractures. Body mass index(BMI; P=0.005) and prevalence of chronic kidney disease(CKD; P=0.002) and rheumatoid arthritis(P=0.001) were significantly different between the groups. In multivariable analysis, BMI, CKD, and RA were associated with an increased risk of sarcopenia, and there were statistically significant differences(P<0.05). Conclusion This study evaluated the prevalence of sarcopenia according to the fracture site and identified associated risk factors such as BMI, CKD, and RA in patients with osteoporotic fractures. [ABSTRACT FROM AUTHOR]

Based on comparison between classical decision-making issues and crisis decision-making issues, specified constrain conditions of crisis decision-making are analyzed, which behaves more like a complex system. In this paper, a decision-making method for complex crisis is developed, which consists of three principles, a cross-simulation model, and a decision-making model based on cross-simulation. The three principles include the dynamic anchoring principle, the effective non-optimal principle, and the bottom line principle. The three principles aim at three major difficulties of crisis decision-making, which are no-fixed objective, lack of evaluation method, and hard to adjust. The cross-simulation model contributes to accuracy and reliability of simulation with integration of data and models. The decision-making model based on cross-simulation provides effective decision support for complex crisis. The method developed in this paper may be applied to more complex issues not limited to crisis. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25 (OH)2D3] on cell proliferation in human glomerular mesangial cells and it′s effects on the regulation of mTOR/p70s6K signaling pathway in this cell line. Methods The cultured human mesangial cells at passage 3-7 were divided into four groups:control group,VD group (addition of 10-8 mol/L of 1,25-dihydroxyvitamin D3 ),R group (addition of 5 mg/L of rapamycin) and R+VD group(addition of 5 mg/L rapamycin combined with 10-8 mol/L of 1,25-dihydroxyvitamin D3). Drug incubation last 48 h. The effect of mesangial cell proliferation was measured by CCK-8 colorimetric assay. The cell cycles were measured by flow cytometry. The expression of mTOR and p70s6K were detected by immunofluorescence. Results (1) The absorbance of A450 was higher in control group than that in VD group than that in R group than that in R+VD group. But the inhibition rate (IR) was lower in control group than that in VD group than that in R group than that in R+VD group. All comparisons were of statistic significance. (2) Cells in G1 phase were higher while cells in G2/M and S phases as well as proliferation rate (PI) were lower in control group than those in VD group than those in R group than those in R+VD group. All comparisons were of statistic significance except in?dexes between group R and group VD. (3) mTOR and p30s6K expressions in mesangial cells were higher in control group than those in VD group than those in R group than those in R+VD group. All comparisons were of statistic significance except indexes between group R and group VD. Conclusion 1,25-dihydroxyvitamin D3 might inhibit mesangial cell proliferation significantly through mTOR/p70s6K signaling pathways. [ABSTRACT FROM AUTHOR]