Objective: To investigate the effects of Shugan Jianpi Huoxue prescription on intestinal mucosal barrier function, oxidative stress response and T helper cell subsets (Th1/Th2) cytokines in patients with ulcerative colitis (UC). Methods: 80 patients with UC who were admitted to our hospital from April 2020 to March 2022 were selected. According to the random number table method, 80 patients with UC were divided into control group (n=40, mesalazin enteric-coated tablets treatment) and study group (n=40, mesalazin enteric-coated tablets combined with Shugan Jianpi Huoxue prescription treatment). The changes of intestinal mucosal barrier function, curative effect, oxidative stress response related indexes, traditional Chinese medicine syndrome score, and Th1/Th2 cytokines were observed in the two groups. Results: The total effective rate in the study group was higher than that in the control group (p<0.05). 2 months after treatment, the scores of diarrhea, chest and hypochondriac distension, god is tired and lazy and belching in the two groups were decreased, and the study group was lower than the control group (P<0.05). Diamine oxidase (DAO), D-lactic acid and endotoxin in the two groups at 2 months after treatment were decreased, and the study group was lower than the control group (P<0.05). 2 months after treatment, the superoxide dismutase (SOD) in the study group was higher than that in the control group, and the malondialdehyde (MDA) was lower than that in the control group (P<0.05). 2 months after treatment, interleukin-4 (IL-4) in the study group was higher than that in the control group, interferon-y (INF-γ) and INF-γ/IL-4 were lower than those in the control group (P<0.05). There was no significant difference in the incidence of adverse reactions in the two groups (P>0.05). Conclusion: Shugan Jianpi Huoxue adjuvant therapy for patients with UC, which can regulate the immune balance of Th1/Th2, improve the body's oxidative stress response and intestinal mucosal barrier function, and promote the improvement of clinical symptoms. [ABSTRACT FROM AUTHOR]