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To investigate the feasibility of livestock wastewater purification by the floating plant Pistia stratiotes L., an experimental study was conducted using P. stratiotes L. treatments of dairy wastewater to clarify the growth status and physiological responses of P. stratiotes L. in different types of wastewater and investigate the purifying effects on nitrogen, phosphorus, and organic matter. The changes in microbial communities were also analyzed using high-throughput sequencing to reveal the relationship between microbial communities and the removal of conventional pollutants. The results showed that the tolerance ranges were 0-2 000 mg·L-1 and 0-750 mg·L-1 for raw and anaerobic and oxidized pond wastewater, respectively[measured as chemical oxygen demand(CODCr)]. When P. stratiotes L. was subjected to high concentrations of wastewater, the chlorophyll content was markedly reduced, and the activities of three enzymes(catalase, peroxidase and superoxide dismutase) were significantly increased. Within the tolerance range, P. stratiotes L. showed good purification effects on the three wastewater types from dairy farms. Pistia stratiotes L. had a rapid purification speed in the first 10 days, which slowly inecreased within 10-20 days. After treatment with P. stratiotes L., the removal rates of total nitrogen, ammonia nitrogen, total phosphorus, and CODCr in raw wastewater were 84.7%-92.7%, 90.6%-96.7%, 30.0%-93.1%, and 67.3%-77.2%, respectively; however, the purification effect of P. stratiotes L. in anaerobic and oxidation ponds decreased slightly. Regarding the microbial communities, Proteobacteria, Firmicutes, and Bacteroidetes, were the main species in different dairy wastewaters. The microbial community structures in the P. stratiotes L. treatment group and non-plant control group were significantly different; in particular, the relative abundance of Cyanobacteria was distinct. The correlation between the microbial community and several indexes of wastewater was significant(P<0.05), indicating that microorganisms played an important role in the removal of pollutants. This study verified the feasibility of P. stratiotes L. in treating wastewater with high concentrations of pollutants and provides a theoretical basis and technical support for dairy farm wastewater treatment. [ABSTRACT FROM AUTHOR]

Because of state actor's bounded rationality,regional integration for institutional cooperation usually encounter double troubles in treaty making and compliance. But in a regional system with a heterogeneous distribution of power, countries participating in collective negotiation for integration will differentiate into three kind including leader(s), followers and free riders, in which the great power seeking leadership will offer credible deterrence to free-riding and treachery by small countries. So the authority of the treaty can be guaranteed. This paper will demonstrate the logic of great power's leadership and the unbalanced approach of regional integration by means of theoretical and empirical research after set up a model of heterogeneous collective activity and a model of social action. Conclusions in this paper can inspire China to participate regional integration and make geopolitical strategy effectively. [ABSTRACT FROM AUTHOR]

Pistia stratiotes L., one types of aquatic plants, has been commonly known as the water lettuce in the arum family. The absorption of Pistia stratiotes L. has been successfully used for the ecological treatment to dispose the urban wastewater in recent years. However, it is still lacking on the removal performance and driving factors of antibiotic resistance genes (ARGs) in the livestock and poultry wastewater. In this study, a systematic investigation was made to evaluate the purification effects and driving factors of ARGs during floating plant Pistia stratiotes L. treating the dairy farm wastewater. An analysis was also made to clarify the overall removal performances and occurrence regularity of ARGs in the different types of wastewater. Furthermore, the measurement was conducted to determine the diversity and structural difference of microbial communities in the ecosystem after different treatments. Thus, the driving factors of ARGs were obtained to combine the microbial communities, mobile genetic elements, and environmental factors. Three kinds of actual wastewaters were set in the dairy farm, including the raw water, anaerobic pond and oxidation pond wastewater. Sixteen kinds of ARGs and three kinds of MGEs were selected to widespread in the wastewater of three dairy farms. The results showed that the excellent overall purification effect of Pistia stratiotes L. was achieved during this time. A positive removal effect was found in the absolute abundance of the most ARGs in the wastewater. The best performance of Pistia stratiotes L. was also achieved for the removal of blaOXA-1 in the raw water, the oxidization pond, the low-concentration anaerobic tank in strB, and the high-concentration anaerobic tank, with the removal effect of 3.66. 3.51, 4.07, and 1.27, respectively. In the raw water, oxidation pond wastewater, and low-concentration anaerobic pond wastewater, the absolute abundance (lg value) of ARGs after the treatment of Pistia stratiotes L. decreased by 0.25 (ermA and fexA) to 3.66 (blaOXA-1), 0.08 (blaTEM-1) to 3.51 (strB), and 0.09 (fexA) to 4.07 (strB), respectively, after the treatment of large flies. By contrast, only 9/16 of the ARGs showed the positive removal for the high concentrations of anaerobic wastewater. There was also the variation in the microbial community in the wastewater after the treatment of Pistia stratiotes L. The high-throughput sequencing demonstrated that the microbial diversity and abundance increased significantly in the different wastewaters. The relative abundance of Proteobacteria and Firmicutes decreased dramatically, but that of Bacteroidetes and Actinobacteriota increased, in the wastewater, which was quite different, compared with the microbial community under plant-free treatment. The microbiome increased by 1.64 times in Flavobacterium at the genus level. The RDA analysis of the main drivers of ARGs showed that the interpretation rates were 9.6%, 6.0%, 7.0%, and 5.1%, respectively, combining with environmental factors with the changes in the ARGs in the combination with the dominant phylum and genus (Hydrogenophaga, Flavobacterium, Bacillus, and Gemmobacter). The network analysis concluded that there was the greatest correlation between the relative abundance of ARGS and the environmental factors in the TP and microbial communities in the Proteobacteria. The mantel test also demonstrated that there was a very significant relationship between the microbial dominant bacteria and individual ARGs. Therefore, the Pistia stratiotes L. can be expected to treat the livestock and poultry wastewater. The removal of ARGS by Macros was also driven by the microbial and environmental factors. The removal effect and driving factors of ARGS can greatly contribute to a theoretical basis for the prevention and control of ARGS pollution in the livestock and poultry sewage. The finding can provide a strong reference to promote the green and sustainable development of plant ecological treatment. [ABSTRACT FROM AUTHOR]

Uncontrollable hemorrhage induced by war or surgery is closely related to pain and high risk of death. Therefore, a timely and effective hemostatic material is highly required for life-threatening traumatic bleeding. However, current hemostatic materials still have various defects and side effects such as slow hemostasis and secondary infection. Herein, gelatin-dopamine-lysine (GDL) and oxidized dextran (ODE) were used to prepare sponge-like hemostasis materials through lyophilization. The results show that GDL/ODE is able to integrate with biological tissue and form physical barriers, which stops bleeding after 60 s of application. GDL/ODE composites show good cyto/hemo-compatibility and highly porous microscopic morphology and water absorption ratio. These results suggest that the developed GDL/ODE sponge is a promising multifunctional hemostasis material for biomedical fields. [ABSTRACT FROM AUTHOR]

Iran is an important political power and strategic hub in the Middle East, connecting Central Asia and West Asia, acting as a significant route from Asia to North Africa and Europe; it is an intersection of the "Silk Road Economic Belt" and the "21st Century Maritime Silk Road". Coupled with the enrichment of petroleum resources and the control of global oil security, Iran's geostrategic position is very important. This study constructs a geo-potential mathematical model and index system from a spatial perspective, including the geoweight evaluation index system, weight distribution, interdependence index, and geo-distance measurements while innovatively incorporating the element of geostrategic investment willingness. Based on these factors, this study conducts a quantitative, comparative analysis of the geo-potential of China, the USA, and Russia in Iran after September 11st, 2001, from two dimensions--"strong-weak" and "positive-negative." Subsequently, the driving factors and mechanisms of geo-potential are obtained through mathematical model construction and empirical research. Finally, by placing Iran on regional and global scales and thoroughly considering both international and regional geopolitical patterns around foreign policy and strategy, we analyze geopolitical relations between China, the USA, Russia, and Iran: diplomatic, economic, and cultural factors considered from a geo-potential perspective. We hope that this study will enrich research on Iran studies and geopolitical studies, through its understanding of the power interaction mechanism and geopolitical relations between global powers and Iran, while providing a research basis to promote the high-quality development of the Belt and Road Initiative. The following conclusions were drawn: (1) The USA's geo-potential in Iran fluctuates frequently in the negative high range, and Russia's geo-potential fluctuates slightly in the positive median range. Fluctuations between these two countries thus show a "strong - strong" interaction. The change of China's geo-potential is relatively separate from that of the USA and Russia, showing an "S" shaped curve trending upward, thus gradually narrowing the gap with Russia. Russia and China have a positive influence on Iran (attraction), while the USA has a negative influence on Iran (repulsion), which is much stronger than the attraction from Russia and China. (2) Geo-diplomacy is the decisive factor guiding the trend of geo-economy and geo-culture. Geo-economy is the direct driving force of the evolution of geo-potential. Geo-culture acts as a collaborative factor; geoeconomy and geo-culture both have a potential influence on geo-diplomacy. (3) Based on amicable geo-cultural relations, the strengthening of China's comprehensive national strength and the deepening of bilateral economic interdependence constantly enhances the intensity of geopolitical relations between China and Iran. The geopolitical relationship between the USA and Iran is occasionally eased but is generally poor. Volatility is largely due to changes in foreign policy triggered by the change of governments, which is the dual response of the domestic political system and the international political environment. The close geopolitical relationship between Russia and Iran is owing to the geographical proximity and the need for a geo-strategy. There is a stable but complex geopolitical relationship network between these two countries, composed of economic, political, and security factors. [ABSTRACT FROM AUTHOR]

Objective To investigate the relationship between cerebrospinal fluid exosome-derived miR-630 and the function of brain microvascular endothelial cells （BMECs） .Methods The subarachnoid hemorrhage endothelial cell model was established to evaluate the effect of hemorrhagic cerebrospinal fluid （BCSF） on BMECs’ proliferation by MTT assay and cell cycle analysis. qRT-PCR and immunofluorescence staining were used to detect the expressions of endothelial cell tight junction protein （ZO-1） and adhesion molecule （ICAM-1 and VCAM-1）. Changes in NOx concentration were detected by radioimmunoassay. The cerebrospinal fluid exosomes in the experimental group （co-incubated with BCSF） and the control group （normal cerebrospinal fluid） were isolated and identified， and differences in the expressions of cerebrospinal fluid exosomal miR-630 between the two groups were compared. BMECs work changes after the intervention with miR-630 analogue were observed. Results The proliferation of BMECs was significantly inhibited in the experimental group； the mRNA and protein levels of ICAM-1， VCAM-1 and ZO-1 were significantly decreased， and the function of endothelial cells was significantly inhibited （P<0.05）. After the successful separation and identification of cerebrospinal fluid exosomes， the expression of miR-630 was significantly lower in the experimental group than in the control group （P<0.05）. The function of BMECs was significantly improved with miR-630 mimics. Conclusion The low expression of miR-630 in cerebrospinal fluid exosomes after subarachnoid hemorrhage is closely related to BMECs injury. [ABSTRACT FROM AUTHOR]

To investigate the coupling effects of unit additional lift duration angle and unit additional lift peak value with the original valve type line peak interval angle on the gas path state parameters, the DK4A diesel engine was taken as the research object, and the different valve type lines were introduced to simulate under the engine speed of 2 800 km·h-1 and 50% load condition.The effects of unit additional lift duration angle, additional lift peak value and original valve profile peak interval angle on the weight of gas path state parameters were analyzed and discussed. The results show that the interval angle between the unit additional lift peak value and the original valve profile peak value is from 100° to 140°, and the additional lift duration angle is from 92° to 132°.The increases of the additional lift peak value and the original valve profile peak interval angle are beneficial to the increase of the influence weight on the air path state parameters, and the influence weights of the unit additional lift peak value and the original valve profile peak interval angle on the change rate of air inlet state parameters are greater than that of the unit additional lift duration angle. [ABSTRACT FROM AUTHOR]

Objective To evaluate the safety and efficacy of Willis covered stent implantation for treating complex vascular diseases in the distal internal carotid artery. Methods 14 patients with complex vascular diseases of the distal internal carotid arteries were treated with 15 Willis covered stents from July 2015 through January 2017. Clinical records including technical success, initial and final digital subtraction angiography (DSA)results, mortality, morbidity and clinical outcome within 3〜18months after treatment were retrospectively analyzed. Results The 14 vascular diseases of the distal internal carotid arteries included blood blister-like aneurysms (8), pseudoaneurysms (3), multiple aneurysms (1), and large wide-necked aneurysms (2). The release sites of Willis covered stents were C7 (3), C6 (8), C5 (2), and C4 (2) segments of the internal carotid arteries. The technical success rate was 100%. In 10 patients, angiography performed immediately after stent release showed complete disappearance of the aneurysm and the parent arteries remained patent. Endoleak was observed in 4 patients, which resolved completely (2) or partially with minor leak (1) after balloon dilatation. Another stent was implanted to eliminate the endoleak on the fourth patient. Occlusion of the ophthalmic artery (2) and anterior choroidal artery (1) occurred after stent implantation with no obvious neurologic symptoms. There were no significant procedural events during the perioperative period. No new neurological deficits were observed on follow-up with mRS score of 0 in 12 patients and 1in2 patients. Postoperative DSA showed patent(13) or mildly stenotic (1) parent arteries, completely occluded (13) or recurrent (1)aneurysms. Conclusion Willis covered stent implantation is safe and effective for treating complex vascular diseases in the distal internal carotid artery with good short and mid-term outcomes. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical characteristics of pseudo-progression occurrence after radiotherapy combined with concomitant temozolomide(TMZ) chemotherapy in glioma Methods The databases of Cochrane Library, Bandolier. PubMed, CNKI and VIP were retrieved by computer. The randomized controlled trials(RCT), cohort studies, case control and ret¬rospective case analysis studies on radiotherapy combined with concomitant TMZ chemotherapy caused pseudo-progression from 1990 to 2014 were collected. After evaluating the literature quality, the clinical characteristics of pseudo-progression were performed the conclusive description. Results One case-control study and 1 case analysis study were included. The results of docu¬ments could be believed. The tumor progress suspected by imaging abnormalities after radiotherapy combined with concomitant TMZ chemotherapy in glioma accounted for 42.4% (36/85 )-53.3%( 24/45) of total patients with radiotherapy and chemotherapy, in which the early pseudo-progression accounted for 50.0%( 18/36)-54.2%( 13/24) ;pseudo-progression was related with the oper¬ative range and had no relation with the radiotherapeutic dose and KPS scores, its relation with the age was uncertain; the median survival period and progression-free period in the patients with pseudo-progression were longer than those in the patients with tu¬mor progression. Conclusion Radiotherapy combined with TMZ chemotherapy has high possibility of psudo-progression, clinic needs to comprehensively consider, especially needs to combined with the patient 's clinical manifestations. [ABSTRACT FROM AUTHOR]

Objective To evaluate the efficacy and safety of percutaneous mechanical thrombectomy (PMT) with the Solitaire AB stent system in acute limb ischemia. Methods 22 patients (16 men, 6 women, age range: 36-87 years, mean 67.4±13.4 years). with acute arterial embolism or thrombosis of limbs demonstrated on digital subtraction angiography were enrolled. The severity of limb ischemia was stratified as Rutherford class I in 2/22 (9.1%), II a in 8/22 (36.4%), and II b in 12/22 (54.5%). All patients underwent PMT with the Solitaire AB stent system together with thrombolysis. Recanalization was assessed by angiography immediately after the procedure. In-hospital complication, 30-day, and long-term follow-up records were reviewed. Results In addition to PMT with the Solitaire AB stent system, 6/22 underwent catheter directed thrombolysis and stenting was performed on 2/22 patients. Successful revascularization defined as thrombolysis in myocardial infarction grade 2 (14/22, 63.6%) or 3 (6,27.3%) was achieved in 20/22 (90.9%) of patients. The 30-day limb salvage and mortality rates were 90.9%and 4.5%, respectively. The results according to Cooley efficacy standard included complete cure in 12/22 (54.5%), good response in 6/22 (27.3%), general improvement in 2/22 (9.1%), poor response or amputation in 2/22 (9.1%), and death in 1/22 (4.5%). Perioperative complications in 3 patients included vasospasm (2) and arterial dissection (1). In the 6-28 month follow-up, limb arterial embolism occurred in 1 patient within 3 months, which resolved with repeated interventional therapy. Toe or lower limb amputation was performed on 2 patients within 1 year with amputation-free survival rate of 81.8%. Conclusion PMT with Solitaire AB stent has favorable early and mid-term limb salvage rates as well as reduced disability and mortality , especially in patients with acute arterial embolism and contraindication to thrombolytic therapy. [ABSTRACT FROM AUTHOR]

Wet (underwater) adhesive materials have wide applications in biomedical and engineering fields. However, a hydration layer on the substrate surface severely prevents the interface interaction between adhesives and substrates, resulting in sharp reduce or even disappearance of the wet adhesion property. Therefore, it is of great significance to prepare adhesive materials with wet adhesion property by removal of the hydration layer via hydrophobic interaction. In this paper, amino acid based monomers, N-acrylylglycine (ACG) and N-acrylyl-L-phenylalanine (ACP) were synthesized, and then poly(Nacrylylglycine-N-acrylyl-L-phenylalanine) hydrogels (P(ACGx-ACPy)) were further prepared via hydrogen cross-linking and chemical cross-linking. The intermolecular interactions and properties of the P(ACGx-ACPy) hydrogels were regulated by adjusting the composition ratio of ACG to ACP. Their adhesive properties were then detected by lap-shear and tensile tests, respectively. The results showed that the hydrogels had well adhesive properties. The maximum wet lap-shear adhesive strength to pig skin could reach 80.2 kPa, when the molar ratio of ACG to ACP of hydrogel was 1∶1. Moreover, the highest compression strength of the hydrogels was 2.2 MPa. Cytotoxicity tests showed that hydrogels had good biocompatibility. The prepared P(ACGx-ACPy) adhesives has the potential applications to bio-tissue and other wet materials repairing. [ABSTRACT FROM AUTHOR]

目的观察2型糖尿病患者腹腔镜Roux-en-Y胃旁路术（LRYGB）后血清胃肠道激素水平变化,并探讨其与手术效果的关系。方法行LRYGB治疗的2型糖尿病患者29例,根据手术疗效分为完全缓解组（组1,16例）和改善组（组2,13例）。收集患者术前及术后12个月血清胃肠道激素[胰高血糖素样肽1（GLP-1）、葡萄糖依赖性促胰岛素激素（GIP）、生长激素释放肽（Ghrelin）和瘦素（Leptin）]检测结果、BMI、FPG、糖化血红蛋白(HbA1C)空腹C肽、空腹胰岛素、胰岛素抵抗指数、胰岛素释放指数等资料,观察手术前后疗效指标及血清胃肠道激素水平变化,比较不同疗效患者血清胃肠道激素水平。结果术后12个月,29例患者BMI、空腹血糖、C肽、HbA1C、空腹胰岛素、胰岛素抵抗指数、血清Leptin水平均较术前降低（P均<0.05）。术后12个月组1血清Leptin水平较术前降低（P<0.05）,组2各项胃肠道激素水平手术前后相比差异无统计学意义。结论 2型糖尿病患者LRYGB术后血清Leptin水平下降,Leptin下降明显的患者手术效果更好。 [ABSTRACT FROM AUTHOR]

In order to improve the finishing process of panel parts, improve the different surface finishing effects, and solve the defects in the finishing process, taking regular thin MDF as the substrate of panel parts, the application of two UV roller coating technologies, closure and opening, in MDF was studied, and the influence factors on the performance of MDF coating film after finishing were analyzed. The results showed that the two roller coating processes of closure and opening gave MDF different surface characteristics, making MDF smooth and flat or with natural wood grain. Due to the different pore sizes of MDF surface after veneer overlaying and plain panel, the parameters of sanding, roller coating and drying process were affected. According to the needs of the surface color of the workpiece, the choice of pigment in the UV coating affected the drying degree of the workpiece. When the UV light absorption intensity of the coating color was large, the curing energy of the drying unit should be increased to supplement the loss of pigment to the UV light. Each working procedure of the process affected the coating film quality of the panel parts after finishing. The process flow and parameters should be flexibly adjusted according to the substrate pore, paints color and technological types. [ABSTRACT FROM AUTHOR]

Based on a certain type of marine diesel electro-hydraulic variable valve system, through the combination of experiment and simulation, the pressure fluctuation evaluation system was established when the simulation results and experimental data were in good agreement. The effects of the opening slope, the maximum opening, the opening phase and the supply pressure of the oil return solenoid valve in plunge interval, stable interval and free drop interval on the characteristic time of instantaneous pressure for the hydraulic oil circuit after solenoid valve were investigated. The results show that the characteristic time of plunge interval is decreased with the increasing of opening slope of oil return solenoid valve and increased with the increasing of the largest opening. The characteristic time of stable interval is increased with the increasing of opening slope of oil return solenoid valve and decreased with the increasing of the largest opening. The effects of the oil return valve open slope and the largest opening on characteristic time of plunge interval and stable interval are greater than those of open phase position and oil-supply pressure. The oil return solenoid valve open slope, the largest opening, the open phase position and the oil-supply pressure have slight effects on the characteristic time of free drop interval. [ABSTRACT FROM AUTHOR]

Objective The performances of PTZ laser gas leakage detection system in natural gas stations are analyzed and evaluated. Methods Fully considering the influence factors such as light intensity attenuation, various field environments, and human interference, a set of operable test methods was designed and actual tests were carried out. Results The detection data under different scenes, different distances and different interference factors were obtained. Conclusions Using the test method proposed in this paper to carry out the field test data shows that the PTZ laser gas leakage detection system has high detection accuracy and strong anti-interference ability. It is generally applicable to all kinds of field environments, and has a good application prospect in the field of gas leakage detection in natural gas stations. [ABSTRACT FROM AUTHOR]

Pistia stratiotes L., a hydrophyte with excellent abilities in removing nitrogen or phosphorus and absorbing heavy metals, has been used widely in sewage purification treatment. To summarize the purification effect of P. stratiotes on pollutants in sewage systematically, this study mainly adopted the literature investigation method and focused on discussing the removal efficiency and analyzing the influencing factors and removal mechanism of P. stratiotes. The results showed that P. stratiotes had strong capacities of nutrient transport, carbon sequestration, and pollutant absorption. Because of the combination of the biological characteristics of P. stratiotes and the role of the system environment and microbes within it, proper usage of the plant provides good application value for the treatment of many kinds of sewage. In addition, the authors also searched for studies on salt or antibiotics removal by P. stratiotes, with the hope of establishing or improving a nutrient absorption and transport model, which could provide guidance for using P. stratiotes in sewage purification treatment. [ABSTRACT FROM AUTHOR]

As the melting of Arctic Sea ice because of global warming creates new shipping routes and energy resources, the post-Cold War peace and stability of the Arctic has been shattered. It has once again become a strategic location for multinational rivalries, attracting widespread attention in international geopolitical circles. Compared to other regions of the world, studying the Arctic region from a Western geopolitical perspective is at the forefront of international research. Although China, as a near-Arctic country, has developed geopolitical research on the Arctic in response to its practical needs, domestic research in this field started late, with room for improvement in its breadth and depth. Therefore, 98 papers in the Web of Science core database that are highly relevant to Arctic geopolitics are selected as the object of the study. Through a literature review, we summarize the development of Arctic research from a Western geopolitical perspective, and the shift in perspective and hot topics; we adopt a forward-looking perspective of future research development trends to provide reference for innovative research in this field in China. The results reveal the following: (1) Research on the geopolitics of the Arctic has been conducted in a wide range of fields and topics in Western academia, with some correlation among the topics, although the links among the studies are not strong, and the research scale generally shows a fluctuating growth trend. (2) The research content, composed of six major fields (boundary and territorial politics, Arctic shipping routes, Arctic resource development, Arctic geo-strategy, Arctic security and governance, and indigenous population issues), focus on politics and economics but is lack of cultural research. (3) Under the influence of aesthetic humanism and the "cultural and institutional turn," the trend in the cross-fertilization of geopolitical, geo-economic, and geo-cultural perspectives has increasingly become more obvious. (4) Research on the Arctic from a geopolitical perspective has been relatively fruitful, although there remain shortcomings in the depth of theoretical research, breadth of research scale, diversity of research methods, and geo-economic and geo-cultural research paradigms, which require further development. Future research will generate new ideas and directions in immaterial areas such as theoretical framework studies, interdisciplinary studies, and emotional culture, and will focus more on the role of multiple actors, such as indigenous peoples and NGOs, in geopolitical knowledge production and power competition. [ABSTRACT FROM AUTHOR]

The Arctic has an important strategic position owing to its rich resources. The world's major nations want to enter the Arctic to participate in Arctic affairs and establish influence in the Arctic; it has become one of the current international hot spots. The current research on geo-setting has turned to the perspective of the national state, but there is little research on this region. Under the background of global warming, China proposed the Arctic policy to build the "Polar Silk Road" with all parties as responsible nations. Based on the framework of geo-setting, this study analyzes the geo-setting of the Arctic and the influence of the "Polar Silk Road" on the geosetting of the Arctic. First, the geo-setting of the Arctic is complex because it is located at the northernmost end of the earth. It contains rich resources and possesses high strategic value, which has led to competition among major nations in the world. As such, the geo-relations in the Arctic involve many subjects and issues. The subject relations include three aspects: the relation among Arctic nations, the relation between the Near-Arctic nations and the Arctic nations, and the relationship between Arctic aborigines and Arctic nations. These issues focus on territory, economy, energy, and the benefits of Arctic aborigines. The geo-structure of the Arctic has experienced three stages of evolution, and now presents a complex structure with the characteristics of "Central-Subcenter-Periphery." Second, the "Polar Silk Road" will influence the geo-settings in the Arctic in two aspects: on the one hand, although some nations disagree with the "Polar Silk Road," it will improve the tense geo-relations in the Arctic, promote the sub-regional cooperation, enable the participation of the Near-Arctic nations in Arctic affairs, and protect the Arctic indigenous people in general. On the other hand, it will make the core of the geo-structure more stable, the tightness among geo-entities closer, and further extend the geo-relations network. [ABSTRACT FROM AUTHOR]

The demand for new engineering talents training has challenged the traditional experimental teaching mode. How to speed up the innovation of engineering education has become an important part of the laboratory reform in universities. This paper, aiming at the existing problems of the traditional experimental teaching and laboratory management, analyzes the necessity of establishing the open engineering laboratory under the new situation, defines the basic principles of open laboratory management and guidance. From the establishment of information management system, introducing students to participate in practical activities, and improving the laboratory management policy three aspects, this paper describes the specific implementation measures and suggestions for construction of open laboratories. This mode not only fully mobilizes students’ initiative in practice and innovation, but also reduces the intensity of laboratory management, and explores a new way for training high-quality engineering talents. [ABSTRACT FROM AUTHOR]

Objective: In order to clone human SLC25A6 into vector pcDNA3.0, and add HA tag to the N terminal, Myc tag to the C terminal respectively, lay the foundation for further study of SLC25A6 as a tool to monitor the mitochondrial import ability. Methods:The PCR primers were designed based on the SLC25A6 mRNA sequence deposited in NCBI and then they were digested by double restriction enzymes and inserted into vector pcDNA3.0. After verification by digestion and sequencing, the constructed plasmid pcDNA3.0-1xHA-SLC25A6-1xMy was transfected into human Hela cells. SLC25A6 expression was verified by Western-blot. Results: The cloned recombination plasmid as tool pcDNA3.0-1xHA-SLC25A6-1xMyc was confirmed by enzyme digestion and DNA sequencing. SLC25a6 gene can be successfully expressed in human Hela cells. Conclusion: Human SLC25A6 tagged with HA in N terminal and Myc in C terminal respectively pcDNA3.0-1xHA-SLC25A6-1xMyc were successfully cloned, and it was well expressed in Hela cells, which provides basic tools for the following studies about SLC25A6 as a tool to monitor mitochondrial import ability and study mitochondrial crucial role in acute kidney injury. [ABSTRACT FROM AUTHOR]

Objective: In order to clone human SLC25A6 into vector pcDNA3.0, and add HA tag to the N terminal, Myc tag to the C terminal respectively, lay the foundation for further study of SLC25A6 as a tool to monitor the mitochondrial import ability. Methods: The PCR primers were designed based on the SLC25A6 mRNA sequence deposited in NCBI and then they were digested by double restriction enzymes and inserted into vector pcDNA3.0. After verification by digestion and sequencing, the constructed plasmid pcDNA3.0-1xHA-SLC25A6-1xMy was transfected into human Hela cells. SLC25A6 expression was verified by Western-blot. Results: The cloned recombination plasmid as tool pcDNA3.0-1xHA-SLC25A6-1xMyc was confirmed by enzyme digestion and DNA sequencing. SLC25a6 gene can be successfully expressed in human Hela cells. Conclusion: Human SLC25A6 tagged with HA in N terminal and Myc in C terminal respectively pcDNA3.0-1xHA-SLC25A6-1xMyc were successfully cloned, and it was well expressed in Hela cells, which provides basic tools for the following studies about SLC25A6 as a tool to monitor mitochondrial import ability and study mitochondrial crucial role in acute kidney injury. [ABSTRACT FROM AUTHOR]

To fullfill the demand of rolling accuracy of magnesium alloy sheets， the symmetric problem during rolling process and the microstructural evolution of the AZ31 sheets with the thickness of 0.5 and 1.0mm， which made by symmetric and asymmetric rolling process using small rollers， were investigated. The results showed that the average grain size in the 0.5mm sheets by symmetric and asymmetric rolling was 10.1 and 8.8μm， respectively， that of the 1.0mm was 16.7 and 13.6μm， respectively. The element equivalent strain of the 0.5mm sheet was up to 0.42 by asymmetric rolling and 0.29 by symmetric rolling and that of the 1.0mm was 0.75 and 0.66， respectively. In symmetric rolling， the distributions of the nodal equivalent Mises stress and shear stress in the 0.5mm sheet were more symmetric by symmetric rolling process， in contrast with those by asymmetric rolling. Grain growth in the 0.5mm sheet by asymmetric rolling was much slower than that by symmetric rolling during annealing for 1h at 250， 300 and 350℃. At 350℃， the average grain size in the 0.5mm sheet by asymmetric rolling was 9.8μm and that by symmetric rolling was 24.9μm. [ABSTRACT FROM AUTHOR]

Submerged spur dike is a kind of typical navigation project regulating structure. During the project implementation, the protection of local scouring near the head is especially important. In order to study the characteristics of local scouring of submerged spur dike head under bottom protection condition, this study adopted undistorted model experiment method and focused on studying the variation response rules of the form of the local scour pit of submerged spur dike head under bottom protection condition, its depth and the bottom protection width. The result indicates that the local scour pit of the head under bottom protection condition is near the bottom protection edge. When bottom protection edge is less than 20 m, the variation of the maximum scour depth is relatively small, but the scout pit's position is away from the dam body. The variation rule of the maximum scour depth with the bottom protection width can be expressed with exponential relation. Based on principle of dimension analysis, this study established a formula to calculate the maximum local scour depth of submerged spur dike under bottom protection condition on riverbed made of sand. [ABSTRACT FROM AUTHOR]

Objective: To clone human Nox4 into a lentiviral over-expression vector pLenti6.3, lay the foundation for further study of Nox4 functions in ROS production. Methods: Based on the Nox4 mRNA sequence deposited in NCBI. Primers were designed, and then they were digested by double restriction enzymes and inserted into the Entry clone pENTR3 C plasmid. After successfully constructing p ENTR3C-Nox4 plasmid, the Nox4 and EGFP tag were inserted into pLenti6.3 expression vector by LR reactions. After verification by digestion and sequencing, the constructed plasmids was transfected into human Hela cells. Nox4 expression were verified by Western-blot and Nox4 cellular localization wes verified by IF based on the EGFP tag signal. Results: The cloned recombination plasmid were confirmed by enzyme digestion and DNA sequencing. Nox4 gene could express in human Hela cells, besides, Nox4 mainly localized in endoplasmic reticulum (ER). Conclusions: The lentiviral vector over-expressed human Nox4 tagged with EGFP were successfully cloned, and it was well expressed in Hela cells, besides, it was mainly localized in the ER, which provided basic tools for the following studies about Nox4 roles for ROS production. [ABSTRACT FROM AUTHOR]

目的探讨早期及术中使用硫酸镁(MgSO4)对重型颅脑损伤患者预后改善的作用。方法选取该院2011 年1月至2014年1月符合标准的重型颅脑损伤患者62例随机纳入对照组（19例）、常规组（18例）和早期及术中应用组 (25例）。对照组不使用任何Mg2+制剂，常规组于入院时使用MgSO4,早期及术中应用组于入院时及术中使用MgSO4;三组 均在入院时、入院后24、48、72h监测患者血清Mg2+浓度，对比患者术前、术后CT平扫结果及伤后3个月的格拉斯哥预 后评分（H0S)。结果早期及术中应用组仅提升伤后24 h血清Mg2+;浓度，且较其他两组高，差异均有统计学意义(P<0.05); 早期及术中应用组患者脑水肿改善情况优于其他两组，差异均有统计学意义(P<0.05);早期及术中应用组患者伤后3个月 H0S中各项目所占比例优于其他两组，差异均有统计学意义(P<0.01或0.05)。结论重型颅脑损伤患者宜在早期及术中应 用MgSO4,可改善患者预后。 [ABSTRACT FROM AUTHOR]