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[Objective] Pineapple is a common tropical fruit, and its ripeness has an important impact on the storage and marketing. It is particularly important to analyze the maturity of pineapple fruit before picking. Deep learning technology can be an effective method to achieve automatic recognition of pineapple maturity. To improve the accuracy and rate of automatic recognition of pineapple maturity, a new network model named MobileNet V3-YOLOv4 was proposed in this study. [Methods] Firstly, pineapple maturity analysis data set was constructed. A total of 1580 images were obtained, with 1264 images selected as the training set, 158 images as the validation set, and 158 images as the test set. Pineapple photos were taken in natural environment. In order to ensure the diversity of the data set and improve the robustness and generalization of the network, pineapple photos were taken under the influence of different factors such as branches and leaves occlusion, uneven lighting, overlapping shadows, etc. and the location, weather and growing environment of the collection were different. Then, according to the maturity index of pineapple, the photos of pineapple with different maturity were marked, and the labels were divided into yellow ripeness and green ripeness. The annotated images were taken as data sets and input into the network for training. Aiming at the problems of the traditional YOLOv4 network, such as large number of parameters, complex network structure and slow reasoning speed, a more optimized lightweight MobileNet V3-YOLOv4 network model was proposed. The model utilizes the benck structure to replace the Resblock in the CSPDarknet backbone network of YOLOv4. Meanwhile, in order to verify the effectiveness of the MobileNet V3-YOLOv4 network, MobileNet V1-YOLOv4 model and MobileNet V2-YOLOv4 model were also trained. Five different single-stage and two-stage network models, including R-CNN, YOLOv3, SSD300, Retinanet and Centernet were compared with each evaluation index to analyze the performance superiority of MobileNet V3-YOLOv4 model. [Results and Discussions] MobileNet V3-YOLOv4 was validated for its effectiveness in pineapple maturity detection through experiments comparing model performance, model classification prediction, and accuracy tests in complex pineapple detection environments. The experimental results show that, in terms of model performance comparison, the training time of MobileNet V3-YOLOv4 was 11,924 s, with an average training time of 39.75 s per round, the number of parameters was 53.7 MB, resulting in a 25.59% reduction in the saturation time compared to YOLOv4, and the parameter count accounted for only 22%. The mean average precision (mAP) of the trained MobileNet V3-YOLOv4 in the verification set was 53.7 MB. In order to validate the classification prediction performance of the MobileNet V3-YOLOv4 model, four metrics, including Recall score, F1 Score, Precision, and average precision (AP), were utilized to classify and recognize pineapples of different maturities. The experimental results demonstrate that MobileNet V3- YOLOv4 exhibited significantly higher Precision, AP, and F1 Score the other. For the semi-ripe stage, there was a 4.49% increase in AP, 0.07 improvement in F1 Score, 1% increase in Recall, and 3.34% increase in Precision than YOLOv4. As for the ripe stage, there was a 6.06% increase in AP, 0.13 improvement in F1 Score, 16.55% increase in Recall, and 6.25% increase in Precision. Due to the distinct color features of ripe pineapples and their easy differentiation from the background, the improved network achieved a precision rate of 100.00%. Additionally, the mAP and reasoning speed (Frames Per Second, FPS) of nine algorithms were examined. The results showed that MobileNet V3-YOLOv4 achieved an mAP of 90.92%, which was 5.28% higher than YOLOv4 and 3.67% higher than YOLOv3. The FPS was measured at 80.85 img/s, which was 40.28 img/s higher than YOLOv4 and 8.91 img/s higher than SSD300. The detection results of MobileNet V3-YOLOv4 for pineapples of different maturities in complex environments indicated a 100% success rate for both the semi-ripe and ripe stages, while YOLOv4, MobileNet V1-YOLOv4, and MobileNet V2-YOLOv4 exhibited varying degrees of missed detections. [Conclusions] Based on the above experimental results, it can be concluded that MobileNet V3-YOLOv4 proposed in this study could not only reduce the training speed and parameter number number, but also improve the accuracy and reasoning speed of pineapple maturity recognition, so it has important application prospects in the field of smart orchard. At the same time, the pineapple photo data set collected in this research can also provide valuable data resources for the research and application of related fields. [ABSTRACT FROM AUTHOR]