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Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the role of AlkB homologue 5 (ALKBH5) mediated m6A methylation modifications in the chemoradiotherapy resistance of cutaneous malignant melanoma (CMM). Methods From May 2019 to January 2021, the expression of ALKBH5 and NFE2L1 (nuclear factor, erythroid 2 like 1) in CMM cells was detected by real-time polymerase chain reaction (RT-PCR). TCGA was used to analyze the expression and correlation of ALKBH5 and NFE2L1. ALKBH5 was knocked down in CMM cells and the changes in m6A methylation expression of NFE2L1 was detected by immunoprecipitation. The role of ALKBH5 and NFE2L1 in chemoradiotherapy resistance of CMM cells was detected by MTT. Results The expression of both ALKBH5 and NFE2L1 in CMM cells was significantly higher than that in normal human epidermal melanocytes (P<0.05). The m6A methylation level of NFE2L1 mRNA was regulated by ALKBH5. In CMM cells with ALKBH5 knocked down, the survival rate of cells decreased and the chemoradiotherapy resistance reduced after irradiation and cisplatin treatment (P<0.05). After overexpression of NFE2L1, the survival rate of cells increased after treatment and the chemoradiotherapy resistance of CMM was induced (P<0.05). Conclusion ALKBH5 promoted chemoradiotherapy resistance of CMM by removing the m6A methylation modification of NFE2L1 and upregulating NFE2L1 expression. The effect of ALKBH5 on malignant phenotype of CMM was mediated through NFE2L1. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the effect of obesity-associated protein (FTO) and human homeobox protein 13(HOXB13) in cutaneous malignant melanoma (CMM) invasion and proliferation. Methods From January 2019 to December 2020, the expression of FTO and HOXB13 in CMM cells was detected by real-time polymerase chain reaction (RT-PCR). The expression and correlation of FTO and HOXB13 was analyzed by TCGA. The changes in expressionof n6-methyladenosine (m6A) of HOXB13 were detected by immunoprecipitation. The biological effects of FTO and HOXB13 on CMM cells proliferation and invasion was analyzed by CCK-8 and Transwell. Results Compared with normal cutaneous malignant melanoma, the expression level of FTO and HOXB13 in CMM cells was significantly increased (P<0.05). The methylation level of m6A of HOXB13 was regulated by FTO. After knocking down FTO in CMM cells, the proliferation and invasion ability was significantly reduced (P<0.05). After overexpression of HOXB13, the proliferation and invasion ability was significantly restored (P<0.05). The effect of FTO on malignant phenotype of CMM was achieved through HOXB13. Conclusion FTO can promote the proliferation and invasion of CMM cells by removing methylation modification of m6A of HOXB13 and up-regulating the expression of HOXB13. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nursing of Integrated Traditional Chinese & Western Medicine is the property of Journal of Clinical Nursing in Practice (Editorial Board, Shanghai Jiao Tong University Press) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nursing of Integrated Traditional Chinese & Western Medicine is the property of Journal of Clinical Nursing in Practice (Editorial Board, Shanghai Jiao Tong University Press) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

王炳元，教授，博士生导师，中国医科大学附属第一医院老年消化内分泌 科主任。中国医师协会脂肪肝专家委员会副主任委员，中国老年医学学会消化分会常委，中 国医药生物技术协会慢病管理分会常委，中华消化学会老年协作组副组长，中华消化学会肝 胆协作组委员，辽宁省免疫学会老年免疫分会主任委员。 [ABSTRACT FROM AUTHOR]

王炳元，教授，博士生导师，中国医科大学附属第一医院老年消化内分泌 科主任。中国医师协会脂肪肝专家委员会副主任委员，中国老年医学学会消化分会常委，中 国医药生物技术协会慢病管理分会常委，中华消化学会老年协作组副组长，中华消化学会肝 胆协作组委员，辽宁省免疫学会老年免疫分会主任委员。 [ABSTRACT FROM AUTHOR]

王炳元，教授，博士生导师，中国医科大学附属第一医院老年消化内分泌 科主任。中国医师协会脂肪肝专家委员会副主任委员，中国老年医学学会消化分会常委，中 国医药生物技术协会慢病管理分会常委，中华消化学会老年协作组副组长，中华消化学会肝 胆协作组委员，辽宁省免疫学会老年免疫分会主任委员。 [ABSTRACT FROM AUTHOR]

王炳元，教授、博士生导师，中国医科大学附属第一医院老年消化内分泌科 主任。中国医师协会脂肪肝专家委员会副主任委员，中华肝病学会脂肪肝和酒精性肝病学组 副组长，中华消化学会老年协作组副组长，中华消化学会肝胆协作组组员，辽宁省消化分会 和辽宁省中西医结合肝病学副主委，辽宁省医学会肝病分会常委，辽宁省免疫学会老年免疫 分会主任委员。 [ABSTRACT FROM AUTHOR]