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目的建立一套五色荧光复合扩增体系，并调查中国北方汉族遗传多态性和父子家系突变情况。方法根据文献筛查出在中国某一群体中突变率为高变异和快速变异的Y-STR基因座，建立一套五色荧光Y-STR复合扩增体系，计算在500个北方汉族无关男性个体中的基因多样性，观察每个样本的Y-STR单倍型，并对中国北方汉族500个父子对进行检测，统计父子间的突变情况。结果成功构建了包含21个Y-STR基因座复合扩增检验体系，在北方汉族群体中基因多态性在0.402 3～0.990 4之间，500个无关个体中未发现有相同的单倍型。在500个父子对中观察到21个Y-STR基因座共发生134次突变，其中突变率最高的基因座是DYF399S1，为7.40×10-2；在DYS612、DYS547、DYS627、DYS526b、DYS576、DYS630、DYS449、DYF404S1、DYS390、DYS570和DYS518基因座上突变率≥1.00×10-2。此外，DYS626、DYS458和DYS439基因座突变率≥4.00×10-3。有97个父子家系出现1个基因座突变，16个家系同时出现2个基因座突变，仅有1个家系同时出现3个基因座突变，家系突变的概率为0.268。其中127次为一步突变，7次为两步突变，符合逐步突变模式。结论高变异Y-STR基因座能够有效区分具有父系亲缘关系的男性个体，可作为联合DNA索引系统（combined DNA index system，CODIS）常染色体和常规低突变Y-STR系统基因座的补充。

[目的]对比研究Amplatzer堵闭器（ADO）与外科手术治疗动脉导管未闭（PDA）的疗效,探讨ADO治疗PDA的临床价值.[方法]1998年11月-2004年1月在我院采用Amplatzer堵闭器介入治疗的PDA患者31例,同期采用外科手术法治疗的PDA患者32例.采用病例对照研究的方法,比较两种方法的疗效、主要并发症等.[结果]两种疗法的成功率（介入组93.3%、外科组100%）、主要并发症发生率（介入组14.3%、外科组12.5%）无显著性差异（P＞0.05）.外科组接受输血、呼吸机辅助通气各8例,介入组无1例接受输血和呼吸机辅助通气;术后住院时间介入组2～7（4.7±1.4）d,外科组7～23（10.9±3.5）d.两组比较均有显著性差异（P＜0.05）.[结论]Amplatzer法治疗PDA安全可靠,疗效与外科手术相似,较外科方法创伤小、术后住院时间短,在适应证范围内,可作为治疗PDA的合适方法.

【目的】提高对川崎病的诊断水平及治疗效果。【方法】对 91 例川崎病的临床表现,影象学特征,治疗及预后进行回顾分析。【结 果】本病以发热、皮疹、肢端变化、黏膜改变、颈淋巴结肿大为临床特征, 67%有冠状动脉病变。本病主要用阿司匹林及大剂量静脉注射丙种球 蛋白治疗。早期误诊率 60%,复发率 4%。【结论】早期诊断、及时治疗、长期随访有重要意义。

【目的】评价Amplatzer 堵闭器对动脉导管未闭(PDA)的治疗效果。【方法】应用Amplatzer 堵闭器和主动脉造影及 6F 长鞘输送器置入Amplatzer 堵闭器, 治疗11 例动脉导管未闭患者。【结果】堵闭成功率100%;随访2 月～ 1 年, 全部病例无 残余分流和任何并发症。【结论】应用Amplatzer 堵闭器治疗PDA 操作简便, 安全, 有效。

To summarize the comprehensive nursing experience in a case of typhoid fever complicated with intestinal bleeding. Based on the nursing risk assessment, comprehensive nursing interventions including guidelines on rest and exercise programs, disinfection protection, psychological nursing, observation and nursing of high fever, observation and nursing of diarrhea, diet nursing and continuous nursing were carried out. Comprehensive nursing interventions were implemented to relieve the symptoms of diarrhea, relieve the pain of the patients, shorten the course of the disease, and prevent cross-infection of the disease. (本文总结1例伤寒并发肠出血患者的综合护理措施。在完善护理评估的基础上, 为患者制定常规的休息与活动计划, 加强消毒防护、心理护理、高热观察及护理、腹泻观察及护理、饮食护理及延续性护理, 旨在缓解患者腹泻症状, 减轻患者痛苦, 缩短病程, 防止疾病交叉感染。)

This paper summarized the whole process of nursing intervention for a patient with oculomotor paralysis caused by systemic lupus erythematosus. Responsible nurses were involved throughout the process, in which overall evaluation of disease condition, routine nursing management, auricular point needle combined with auricular point pressing therapy were carried out. Targeted nursing plan should be made based on the overall evaluation and Traditional Chinese Medicine syndrome differentiation, in order to improve the rehabilitation of the patient. (本文总结1例系统性红斑狼疮(SLE)致动眼神经麻痹患者的护理经验, 包括整体评估、常规护理、耳针联合耳穴贴压等, 根据患者的评估结果和中医辨证, 制定针对性的护理方案, 有助于促进患者康复。)

Objective To explore the clinical effect of diversified nursing intervention on the prevention and treatment of postoperative urinary retention in patients with mixed hemorrhoids. Methods From October 2018 to June 2019, 60 patients undergoing surgery for mixed hemorrhoids were selected and randomly divided into control group (n=30) and observation group (n=30)according to the random number table method. The control group received conventional nursing intervention, and the observation group was treated with diversified nursing interventions. The total effective rate, time of first urination, volume of first urination, incidence of postoperative urinary retention and bladder residual urine volume (RUV) were evaluated respectively. Results The time of first urination in the observation group was shorter than that in the control group(P＜0. 01). The volume of first urination in the observation group was higher than that of the control group(P＜0. 01). Postoperative bladder RUV in the observation group was lower than that in control group (P＜0. 01). The incidence of urinary retention in the observation group was lower than that in the control group(P＜0. 01). The total effective rate of the observation group was higher than that of the control group (96. 67% vs. 70. 00%, P＜0. 01). Conclusion Diversified nursing interventions are potentially effective to relieve dysuresia symptoms and reduce the risk of urinary retention after surgery for mixed hemorrhoids. (目的 探讨多元化护理干预防治混合痔术后尿潴留的效果。方法 选取2018年5月—2019 年6月收治的60例行混合痔手术治疗的患者为研究对象, 采用随机数字表法分为对照组与观察组。对照组30例采用常规护理干预, 观察组30例采用多元化护理干预。评价干预效果, 对比两组患者术后首次排尿时间、术后首次尿量、术后尿潴留发生率、膀胱内残余尿量。结果 观察组术后首次排尿时间短于对照组, 术后首次尿量高于对照组, 术后膀胱内残余尿量(RUV)低于对照组, 尿潴留发生率低于对照组, 差异有统计学意义(P＜0. 01)。观察组总有效率96. 67%, 高于对照组的70. 00%, 差异有统计学意义(P＜0. 01)。结论 多元化护理干预可有效改善患者排尿困难, 降低混合痔术后尿潴留发生风险。)

This paper summarized the illness observation and nursing experience in a case of mass diarrhea. Key issues of nursing interventions for diarrhea included illness condition observation, auricular point pressing therapy, moxibustion; psychological care; diet guidance; disinfection and protection, health education and extended care. The integrated traditional Chinese and western medicine nursing is effective to relieve the diarrhea symptoms, reduce the pain degree and improve the rehabilitation of patients. (本文总结1起群体性腹泻的病情观察及护理体会, 包括病情观察、耳穴贴压、艾条灸、心理护理、饮食指导、消毒防护、健康宣教、延续性护理等措施。中西医结合护理能有效缓解患者腹泻症状, 减轻痛苦, 促进患者康复。)

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In view of the security and privacy protection issues faced in sharing sensing information data between sensing devices in the internet of things, this study proposes a blockchain based multi domain data sharing mechanism. The mechanism treats the data sharing problem as a data transaction problem and introduces a trusted regulator to guarantee the interests of both buyers and sellers of the transaction. The mechanism provides a data publishing method in which buyers can securely publish data to a cloud storage service and do not disclose information about the data during the publishing process. A ring signature is used to sign and verify the buyer's licence in order to protect the buyer's privacy from being compromised during the data transaction. In addition, the mechanism provides an anonymous evaluation method that allows buyers to evaluate transactions and monitor seller behaviour without revealing their identity. The security analysis and simulation experiments results show that the mechanism is secure and efficient, meets the requirements for secure data sharing in the internet of things. [ABSTRACT FROM AUTHOR]

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As is well known, experimental teaching is crucial for cultivating studentsʼ practical and innovative abilities. How to overcome the spatial and temporal limitations of classroom teaching and ensure the open utilization of experimental resources is an important task for all the experimental teaching centers in Chinese universities. This article takes the National Experimental Teaching Demonstration Center for Chemistry (Beijing Normal University) as an example to introduce the multi-level and multidimensional laboratory access strategies. The center incorporates the well-organized “second classroom activities” into the experimental teaching system for supporting undergraduates to actively participate various innovation and entrepreneurship activities, academic competitions, scientific research, and science popularization. These initiatives provide students with diverse, self-driven, and innovative-focused practical training opportunities, effectively strengthening the role of experimental teaching centers in cultivating innovative talents and serving as the demonstration base for basic education. These efforts have proven to be effective and have significant educational outcomes, making them worthy of wider adoption. [ABSTRACT FROM AUTHOR]

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Zinc deficiency during pregnancy is common and has been significantly associated with adverse pregnancy outcomes such as spontaneous preterm birth(PTB), recurrent abortion(RA), low birth weight(LBW), small-for-gestational-age infant(SGA), preeclampsia(PE), and gestational diabetes mellitus(GDM). Unfortunately, there is no specific biomarkers which are sensitive, easy-to-collect and detect available for the clinical evaluation of zinc nutritional status. However, recent studies have identified metallothionein and the ratio of oleic acid to dihydroxymethyl gamma-linolenic acid(LA/DGLA) as potentially candidates of biomarkers.This article focuses on summarizing and discussing the progress of domestic and international researches on zinc deficiency during pregnancy and adverse pregnancy outcomes, potential zinc biomarkers, and zinc deficiency treatments, aiming at providing ideas for perinatal nutritional guidance. [ABSTRACT FROM AUTHOR]

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This experimental case aims to address the limitations of traditional Fe(OH)3 colloid electrophoresis experiments, such as the waste of Fe(OH)3 colloids, the lack of exploration, and the difficulty in incorporating ideological and political education. To do so, we have developed a colloidal dissolution method, introduced AgI colloids with different surface charges and designed colloid surface charge exploring assays, thus rendering this experiment with the features of resource conservation, green development, and richness of inquiry. It is particularly important that this case has integrated multiple teaching modes (e.g., task-driven self-learning, flipped classroom, and deliberative teaching) to seamlessly combine this basic experiment with the improvement of the recycling ratio of China’s steel resources and the correction to students’ inherent knowledge on the surface charges of colloids. As a result, green chemistry concept, sustainability responsibility, scientific dialectical thinking skills, and innovation spirit can be implanted into the mind of students in a nature way during this experiment. This case has been initially applied to the colloid electrophoresis experimental taught in our university, achieving excellent teaching responses and mutual promotion effect between value shaping, knowledge imparting and ability cultivation. Hopefully, this case can provide helpful reference for enhancing the effect of professional education and ideological and political education in basic experiments. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical characteristics and postoperative follow-up of gliosarcoma (GSM). Methods The clinical data of 13 patients with GSM who underwent surgery at the Affiliated Hospital of Guizhou Medical University from August 2012 to July 2020 were retrospectively analyzed, including basic clinical features, preoperative imaging features, and postoperative molecular pathological findings. Follow-up was performed, until the patient died. Results 8 male and 5 female patients with GSM were collected. The average age was 51.69 years old, ranging from 23 to 70 years old. The chief complaints of most patients were dizziness and headache, and the mainly located on the supratentorial region. Preoperative MRI showed that the lesions were mainly lumpy in T1WI and T2WI. T1 enhanced MRI revealed annular enhancement or irregular enhancement. All the pathological diagnosis after operation indicated both glioblastoma components and sarcoma components, and reticulofibers staining was positive, staining by positive cells of glial fibrillary acidic protein, vimentin, S-100 and Olig-2. Postoperative radiotherapy was 3 cases and temozolomide chemotherapy was 8 cases. Postoperative follow-up revealed recurrence in 5 cases, including 3 of primary focus recurrence, 1 of extracranial metastasis via cerebrospinal fluid, and 1 of metastasis to medulla oblongata and the fourth ventricle, with 8.47 months of the overall median survival. Conclusions GSM,mainly diagnosed by pathological features, was rare and relapsed easily. Generally, GSM transfers to adjacent brain tissues and metastasized natural anatomical pathways, with poor prognosis. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Drainage & Irrigation Machinery Engineering / Paiguan Jixie Gongcheng Xuebao is the property of Editorial Department of Drainage & Irrigation Machinery Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The aims of this study are to explore the ecosystem service trade-offs and synergistic relationships in Ruili-Daying River basin, and to provide a theoretical basis for land management and ecosystem service optimization in this region. [Method] The InVEST model was used to quantitatively evaluate the ecosystem service of water yield, soil retention, and carbon storage in Ruili-Daying River Basin based on multi-source data including the land use and land cover, topography, meteorology and soil conditions covering 2000, 2010 and 2020. Moreover, the characteristics of trade-offs and synergies among different ecosystem services were analyzed by using the spatial overlay analysis method. [Results] (1) From 2000 to 2020, the areas of forest land, cropland, grassland, wetland and bare land decreased in Ruili-Daying River basin, while the shrubland, construction land and water bodies increased gradually. (2) The mean water supply depth, unit amount of soil retention and carbon storage were 749.60 mm, 365.78 t/hm-2, 137.51 t/hm-2, respectively. In the past 20 years, the water yield and soil retention increased at first and then decreased, and the change of carbon storage was not obvious. (3) The ecosystem services provided by different land use types differentiated, with construction land, shrubland and forest land providing the highest water yield, soil retention capacity and carbon storage per unit area, respectively. (4) The ecosystem service relationship in Ruili-Daying River Basin was dominated by trade-offs, in which the proportion of severe trade-off areas was as high as 55%, the proportion of weak trade-off areas was about 4%～8%, the proportion of areas with poor synergy was about 24%～30%, and the proportion of areas with good synergy was about 10%～13%. During the study period, the proportion of with poor synergistic areas decreased by 5.78%, while the proportion of weakly trade-off, severely trade-off and well synergistic areas increased by 2.79%, 0.79% and 2.2%, respectively. [Conclusion] The degree of ecosystem service trade-offs in Ruili-Daying River basin was enhanced while the overall level of services was on an upward trend. [ABSTRACT FROM AUTHOR]