Search

Copyright of Biotechnology Bulletin is the property of Biotechnology Bulletin Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ｇｕａｎｇｘｉ Ｔｏｎｇｍｉａｎ ｉｓ ａｎ ｉｍｐｏｒｔａｎｔ ｓｕｐｅｒｉｏｒ ｐｒｏｖｅｎａｎｃｅ ｒｅｇｉｏｎ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ ｉｎ Ｃｈｉｎａ. Ｔｈｅ ｄｉｓｔｒｉｂｕｔｉｏｎ ａｒｅａ ｏｆ ｔｈｉｓ ｐｒｏｖｅｎａｎｃｅ ｈａｄ ｒｅａｃｈｅｄ １５ ６００ ｈｍ２. Ｉｔ ｈａｄ ａ ｈｉｇｈ-ｑｕａｌｉｔｙ ａｎｄ ｒｉｃｈ ｇｅｎｅｔｉｃ ｒｅｓｏｕｒｃｅｓ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ, ａｎｄ ｍａｄｅ ａ ｇｒｅａｔ ｃｏｎｔｒｉｂｕｔｉｏｎ ｔｏ ｔｈｅ ｇｅｎｅｔｉｃ ｉｍｐｒｏｖｅｍｅｎｔ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ. Ｏｖｅｒ ｔｈｅ ｌａｓｔ ３０ ｙｅａｒｓ, ｎａｔｕｒａｌ ｒｅｓｏｕｒｃｅｓ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ ｉｎ ｔｈｉｓ ｒｅｇｉｏｎ ｈａｖｅ ｓｕｆｆｅｒｅｄ ｓｅｒｉｏｕｓ ｄａｍａｇｅ. Ｔｏ ｕｎｄｅｒｓｔａｎｄ ｔｈｅ ｇｅｎｅｔｉｃ ｄｉｖｅｒｓｉｔｙ ｏｆ ｎａｔｕｒａｌ ｇｅｒｍｐｌａｓｍ ｒｅｓｏｕｒｃｅｓ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ ｉｎ ｔｈｉｓ ａｒｅａ, ＳＳＲ ｍｏｌｅｃｕｌａｒ ｍａｒｋｅｒｓ ｗｅｒｅ ｕｓｅｄ ｔｏ ａｎａｌｙｚｅ ｔｈｅ ｐｏｐｕｌａｔｉｏｎ ｇｅｎｅｔｉｃ ｓｔｒｕｃｔｕｒｅ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ ｉｎ Ｔｏｎｇ-ｍｉａｎ ｒｅｇｉｏｎ. Ｔｈｅ ｒｅｓｕｌｔｓ ｓｈｏｗｅｄ ｔｈａｔ ５３ ａｌｌｅｌｅｓ ｗｅｒｅ ｉｄｅｎｔｉｆｉｅｄ ｂｙ １６ ｐａｉｒｓ ｏｆ ＳＳＲ ｐｒｉｍｅｒｓ ｉｎ ２８５ ｓａｍｐｌｅｓ, ａｎｄ ｔｈｅ ｐｏｌｙ-ｍｏｒｐｈｉｓｍ ｒａｔｅ ｗａｓ １００％. Ｔｈｅ ｍｅａｎ ｎｕｍｂｅｒ ｏｆ ａｌｌｅｌｅｓ (Ｎａ), ｔｈｅ ｍｅａｎ ｎｕｍｂｅｒ ｏｆ ｅｆｆｅｃｔｉｖｅ ａｌｌｅｌｅｓ (Ｎｅ), Ｓｈａｎｎｏｎ'ｓ ｉｎ-ｆｏｒｍａｔｉｏｎ ｉｎｄｅｘ (Ｉ), ｏｂｓｅｒｖｅｄ ｈｅｔｅｒｏｚｙｇｏｓｉｔｙ (Ｈｏ), ｅｘｐｅｃｔｅｄ ｈｅｔｅｒｏｚｙｇｏｓｉｔｙ (Ｈｅ) ｗｅｒｅ ３.３１, １.６８, ０.６４, ０.３５ ａｎｄ ０.３６. Ｔｈｉｓ ｓｈｏｗｅｄ ｔｈａｔ ｔｈｅ Ｔｏｎｇｍｉａｎ ｐｏｐｕｌａｔｉｏｎ ｓｔｉｌｌ ｍａｉｎｔａｉｎｅｄ ｈｉｇｈ ｌｅｖｅｌ ｏｆ ｇｅｎｅｔｉｃ ｄｉｖｅｒｓｉｔｙ. Ａｎａｌｙｓｉｓ ｏｎ ｇｅｎｅｔｉｃ ｓｔｒｕｃ-ｔｕｒｅ ｏｆ ｅａｃｈ ｓｔａｎｄ ｉｎ Ｔｏｎｇｍｉａｎ ｐｏｐｕｌａｔｉｏｎ ｓｈｏｗｅｄ ｔｈａｔ ｃｏｅｆｆｉｃｉｅｎｔ ｏｆ ｇｅｎｅｔｉｃ ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ (ＧＳＴ), ｆｉｘａｔｉｏｎ ｉｎｄｅｘ (ＦＳＴ) ａｎｄ ｇｅｎｅ ｆｌｏｗ(Ｎｍ) ｗｅｒｅ ０.０４９, ０.０７２ ａｎｄ ３.２１. Ｔｈｅ ｇｅｎｏｔｙｐｅ ｄｉｓｔｒｉｂｕｔｉｏｎ ｗａｓ ｃｌｏｓｅｒ ｔｏ Ｈａｒｄｙ-Ｗｅｉｎｂｅｒｇ ｅｑｕｉｌｉｂｒｉｕｍ, ａｎｄ ｈａｄ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｈｅｔｅｒｏｚｙｇｏｔｅ ｅｘｃｅｓｓ ｏｒ ｓｈｏｒｔａｇｅ. Ｉｎ Ｔｏｎｇｍｉａｎ ｐｏｐｕｌａｔｉｏｎ, ｍｏｓｔ ｇｅｎｅｔｉｃ ｖａｒｉａｔｉｏｎ ｍａｉｎｌｙ ｅｘｉｓｔｅｄ ｗｉｔｈｉｎ ｔｈｅ ｓｔａｎｄ, ａｎｄ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｇｅｎｅｔｉｃ ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ ｅｘｉｓｔｅｄ ａｍｏｎｇ ｓｔａｎｄｓ. Ｔｈｅ ｇｅｎｅ ｆｌｏｗ ｉｎ ｉｔ ｗａｓ ｓｍｏｏｔｈ. Ｍｅａｎ-ｗｈｉｌｅ, ｔｈｅ ｇｅｎｅｔｉｃ ｄｉｖｅｒｓｉｔｙ ｏｆ ｔｈｅ ｆｏｕｒ ｓｔａｎｄｓ ｉｎ ｔｈｅ ｃｏｒｅ ｒｅｇｉｏｎ ｗａｓ ｌｏｗｅｒ ｔｈａｎ ｇｅｎｅｔｉｃ ｄｉｖｅｒｓｉｔｙ ｏｆ ｔｈｒｅｅ ｓｔａｎｄｓ ｏｕｔｓｉｄｅ ｔｈｅ ｃｏｒｅ ｒｅｇｉｏｎ. Ｔｈｉｓ ｉｎｄｉｃａｔｅｄ ｔｈａｔ ｔｈｅ ｃｏｒｅ ｒｅｇｉｏｎ ｏｆ Ｔｏｎｇｍｉａｎ ｐｒｏｖｅｎａｎｃｅ ｓｕｆｆｅｒｅｄ ｍｏｒｅ ｓｅｒｉｏｕｓ ｍａｎ-ｍａｄｅ ｄａｍａｇｅ. Ｉｎ ｏｒｄｅｒ ｔｏ ｐｒｏｔｅｃｔ ｔｈｅ ｎａｔｕｒａｌ ｕｐｄａｔｉｎｇ ａｎｄ ｎａｔｕｒａｌ ｇｅｎｅｔｉｃ ｉｍｐｒｏｖｅｍｅｎｔ ａｂｉｌｉｔｙ ｏｆ ｔｈｅ ｎａｔｕｒａｌ ｐｏｐｕｌａｔｉｏｎｓ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ ｉｎ ｔｈｉｓ ａｒｅａ, ｗｅ ｓｈｏｕｌｄ ｐａｙ ａｔｔｅｎｔｉｏｎ ｔｏ ｔｈｅ ｓｃｉｅｎｔｉｆｉｃ ｍａｎａｇｅｍｅｎｔ ｏｆ ｎａｔｕｒａｌ ｆｏｒｅｓｔ. Ｏｎ ｔｈｅ ｏｎｅ ｈａｎｄ, ｇｅｎｅｔｉｃ ｒｅｓｏｕｒｃｅｓ ｓｈｏｕｌｄ ｂｅ ｃｏｌｌｅｃｔｅｄ ａｎｄ ｌａｒｇｅ-ｓｃａｌｅ ｇｅｒｍｐｌａｓｍ ｒｅｓｏｕｒｃｅｓ ｓｈｏｕｌｄ ｂｅ ｅｓｔａｂｌｉｓｈｅｄ. Ｏｎ ｔｈｅ ｏｔｈｅｒ ｈａｎｄ, ｗｅ ｓｈｏｕｌｄ ｅｓｔａｂｌｉｓｈ ｓｐｅｃｉａｌ ｎａｔｕｒｅ ｒｅｓｅｒｖｅ ａｎｄ ｐｒｏｈｉｂｉｔ ｉｌｌｅｇａｌ ｆｅｌｌｉｎｇ, ｆｉｎａｌ ｆｅｌｌｉｎｇ, ｉｌｌｅｇａｌ ｒｅｓｉｎ ｔａｐｐｉｎｇ ａｎｄ ｅｘｃｅｓｓｉｖｅ ｓｅｅｄｉｎｇ ｆｏｒ ｔｈｅ ｎａｔｕｒａｌ ｐｏｐｕｌａｔｉｏｎ ｌｉｋｅ Ｔｏｎｇｍｉａｎ ｗｈｉｃｈ ｈａｓ ｌａｒｇｅ ｄｉｓｔｒｉｂｕｔｉｏｎ ａｒｅａ, ｈｉｇｈ ｕｔｉｌｉｚａｔｉｏｎ ｖａｌｕｅ ａｎｄ ｎｏ ｓｅｒｉｏｕｓ ｄａｍａｇｅ ａｔ ｐｒｅｓｅｎｔ. Ｔｈｅ ｒｅｓｕｌｔｓ ｏｆ ｔｈｉｓ ｓｔｕｄｙ ｈａｖｅ ｉｍｐｏｒｔａｎｔ ｒｅｆｅｒｅｎｃｅ ｖａｌｕｅ ｆｏｒ ｔｈｅ ｒｅｓｅａｒｃｈ ａｎｄ ｐｒｏｔｅｃｔｉｏｎ ｏｆ ｔｈｅ ｎａｔｕｒａｌ ｇｅｒｍｐｌａｓｍ ｒｅｓｏｕｒｃｅｓ ｏｆ ｍａｓｓｏｎ ｐｉｎｅ. [ABSTRACT FROM AUTHOR]

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Protein S-acylation is a common and unique reversible way in posttranslational lipid modification, thus confers diverse physiological functions on target proteins, such as DHHC(Asp-His-His-Cys)protein family. There was evidence that the DHHC domain was directly involved in the palmitoyl transfer reaction. In this paper, we reports gene cloning and expression analysis of DHHC protein family genes in Liriodendron chinense. Three full-length cDNA of DHHC gene were cloned from Liriodendron leaf buds using the rapid amplification of cDNA ends(RACE)strategy, named LcPAT7, LcPAT22 and LcPAT23 respectively. The full-length cDNAs of LcPAT7, LcPAT22 and LcPAT23 were 1 933 bp, 2 592 bp and 2 217 bp, and contain 1 332 bp, 1 839 bp and 1 662 bp ORF, encoding 433, 612 and 533 amino acids, respectively. The predicted molecular weights of the proteins encoded by LcPAT7, LcPAT22 and LcPAT23 were 40.04, 67.3 and 60.57 kDa, respectively. The predicted isoelectric points are 9.15, 9.03 and 7.29, respectively. All the three proteins contained four putative transmembrane(TM)domain structures, and contained a typically DHHC-CRD domain between TM2 and TM3 as most known DHHC protein. Homology analysis showed that the three genes showed high similarity with predicted PAT(protein S-acyltransferase)of other plants. Tissue expression profile by Real-time PCR showed that all the three genes were expressed in various tissues, although the expression levels varied significantly in different tissues. Changes in gene expression patterns in the same gene family indicated their functionally non-redundancy. These results above will provide clues for exploring the underlying mechanism of gene regulation on growth, development, morphogenesis and signal transduction of stress response in L. chinense. [ABSTRACT FROM AUTHOR]