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Based on the comprehensive index including Taobao village economic force, rural environment foundation, e-commerce support, electricity information security, this paper analyzes the temporal-spatial pattern and its influencing factors of rural e-commerce development level in Zhejiang province by using the comprehensive evaluation method and ESDA method. The results indicate that: (1) At the provincial level, rural e-commerce in Zhejiang province is on the first class in China and plays an obvious leading and demonstration role. In terms of temporal characteristics, it shows a growth trend of "fast-stable-fast". It is worth mentioning that Zhejiang and its surrounding areas induce an agglomeration effect in the Yangtze River Delta Region. (2) At the city level, the high-level areas are Hangzhou, Ningbo, Jiaxing, Taizhou and Wenzhou, demonstrating a positive agglomeration effect in the coastal areas. The rural e-commerce level of Jinhua, Shaoxing and Huzhou is relatively not so high, and the peripheral areas near Jiangxi are on the relatively low e-commerce level. (3) At the county level, the high-level areas are mainly distributed in two places, one is in the central-northern area with Hangzhou urban area, Ningbo urban area and Yiwu city as the fulcrum, and the other is located in the southeast Zhejiang with Wenzhou urban area and Taizhou urban area as the core. The low-level areas are mainly distributed in the southwest Zhejiang with low-value agglomeration effect. (4) At the township level, the distribution of taobao village agglomeration areas are similar to the overall pattern at city and county level, they are mainly located in the central-northern regions and the southeastern coastal areas. The internal specialized organization and proximity diffusion effect of high-level units are obvious, while the peripheral areas in the Western and Southwest China are not well developed. High-level taobao villages have formed three core areas with the product of hardware appliances, small household appliances and clothing and fur industries in northern, central and southeast Zhejiang respectively. (5) From the perspective of influencing factors, at the city and county level, the innovative talents, economic support and other indicators of positive impact as well as industrial level of negative impact is prominent, at the county level, the industry and wholesale and retail also play a role together, while at the township level, the taobao village agglomeration and the basis for the growth are more explanatory.(从淘宝电商经济、乡村经济环境、电商经济支撑、电商服务保障4个维度构建指标体系，运用综合评价法、探索性空间数据分析（exploring spatial data analysis，ESDA）法，从省、市、县、乡镇4种尺度对浙江省农村电商发展水平的时空分异规律进行探索，并运用空间回归分析法进行影响因素分析。研究表明：（1）从省域尺度看，浙江省农村电商水平处于全国第一梯队且引领示范作用明显，在时间上表现为“快-稳-快”的增长趋势。江浙沪区域，在空间上与周边沿海城市产生了“近朱者赤”的高-高集聚效果。（2）从市域尺度看，高水平区主要分布在杭州市、宁波市、嘉兴市、台州市和温州市，并在沿海地区形成正向集聚，金华市、绍兴市和湖州市集聚效应相对不高，靠近江西省的外围区城市发展水平相对较低。（3）从县域尺度看，高水平区主要分布在两处，一处是以杭州市、宁波市、义乌市为支点的中北部区域，另一处是在以温州市、台州市为支撑的东南部集聚片区。低水平区主要分布在浙西南地区并产生了低值集聚效应，形成塌陷区。（4）从乡镇尺度看，淘宝村集聚区与市域、县域尺度类似，主要分布在浙中北地区和浙东南沿海一带，内部的专业化组织、临近扩散效应明显，而在浙西、浙西南外围区发育不佳。高、较高水平淘宝村在浙北、浙中、浙东南地区分别形成了以五金电器、小家电和服装皮草业为核心的“一主两副”3个核心区。（5）从影响因素看，市、县尺度均表现为创新人才、经济支撑等指标的正向影响和工业水平的负向影响突出，县域尺度主要受工业和批发零售业共同作用，乡镇尺度下淘宝村的集聚水平与成长基础更具解释性。)

对2根螺栓连接波纹腹板钢梁试件进行疲劳试验研究，获得了钢梁试件的疲劳性能指标以及失效破坏形态，分析了钢梁截面的应力分布以及循环荷载作用下的应力和刚度变化。汇总现有的焊接波纹腹板钢梁疲劳试验结果，比较分析了焊接钢梁与螺栓连接钢梁的疲劳寿命差异。采用有限元软件ABAQUS建立了螺栓连接波纹腹板钢梁的精细数值模型，根据疲劳试验结果验证了数值模型的准确性。利用经验证的数值模型，对疲劳破坏位置附近的应力分布进行有限元分析，结果表明螺栓孔边应力集中是疲劳裂纹萌生和引发疲劳破坏的主要原因。基于有限元分析结果，利用FE-Safe软件对螺栓连接波纹腹板钢梁试件的疲劳寿命进行分析，计算得到的疲劳寿命和疲劳破坏位置与试验结果吻合良好。

Copyright of Machine Tool & Hydraulics is the property of Guangzhou Mechanical Engineering Research Institute (GMERI) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The receptor-binding domain (RBD) of SARS-CoV-2 is a key target for recombinant protein vaccines. Efficient, high-quality expression of RBD protein is crucial for developing effective recombinant protein vaccines. Compared to RBD vaccines produced in CHO cells, plant expression systems offer advantages of higher safety and lower cost. However, current RBD antigen expression levels in plants are relatively low, and methods for extraction and quality optimization have not been fully established. This study used amino acids R319~K537 of the SARS-CoV-2 prototype strain RBD as a template for transient expression in Nicotiana benthamiana using the plant expression vector pCAMBIA1300.The initial expression levels were low at 23.3 μg/g fresh weight, accounting for 0.23% of total soluble plant protein. There was a 2.61-fold increase of RBD expression when it was co-expressed with the efficient gene silencing suppressor pGD-VSRs. By fusing green fluorescent protein to the C-terminus, we localized RBD expression to the apoplastic region in plants. Further introduction of the αAmy3 signal peptide improved RBD accumulation with a 2.1-fold compared to the PR1a signal peptide. Ultimately, RBD expression in N. benthamiana was enhanced to 128.6 μg/g fresh weight, constituting 1.29% of total soluble plant protein. Subsequently, we employed an apoplastic extraction and purification method to obtain RBD protein with over 90% purity. Mouse immunization experiments demonstrated that this plant-derived RBD has comparable immunogenicity to CHO-derived RBD. This study provides important theoretical foundation and technical support for the development of plant-derived RBD vaccines. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Zhejiang University (Science Edition) is the property of Journal of Zhejiang University (Science Edition) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Urban Architecture & Development is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Jilin University (Science Edition) / Jilin Daxue Xuebao (Lixue Ban) is the property of Zhongguo Xue shu qi Kan (Guang Pan Ban) Dian zi Za zhi She and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Arid Zone Research / Ganhanqu Yanjiu is the property of Arid Zone Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Liaoning Technical University (Natural Science Edition) / Liaoning Gongcheng Jishu Daxue Xuebao (Ziran Kexue Ban) is the property of Journal of Liaoning Technical University (Natural Science) Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective The transcriptome sequencing results of brain tissues of olanzapine-treated mice were analyzed to screen out differentially-expressed genes and explore potential targets of atypical antipsychotics leading to body weight gain. Methods Twenty female C57BL/6 mice were randomly divided into control group (Ctrl) and Olanzapine administration group (Olz), which were given saline and Olanzapine solution by gavage, respectively. The whole brain tissues were collected 8 weeks later for Transcriptome sequencing (RNA-Seq). The possible targets of olanzapine-induced body weight gain were identified by the Gene Ontology (GO) functional annotation analysis, the Kyoto Encyclopedia of Genes and Gnomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) network analysis. Differential expression levels of mRNAs were further verified by real-time quantitative fluorescence PCR (RT-qPCR). Results Compared with Ctrl group, 591 differentially expressed genes were screened in Olz group, including 251 upregulated genes and 340 down-regulated genes. GO analysis showed that differential genes were widely involved in transcriptional process, among which the expression of genes related to the regulation of digestive system and cold-induced thermogenesis were significantly enriched. KEGG analysis showed that differential genes were widely involved in the interaction between neuroactive ligands and receptors, and the differential genes were significantly enriched in oxytocin signaling, fat digestion and absorption, and cholesterol metabolism pathways. RT-qPCR were performed to verify the expression levels of genes enriched in feeding regulation, gastric kinesis, thermogenesis, fat metabolism and other processes (Oxt, Trpv1, Adipoq, Phox2b, Abcg5, Mogat2, Dbh, Plac8 and Neurog1) as well as hub genes in PPI network (Fos, Dusp1 and Egr2), and the results were consistent with the trend of RNA-Seq. Conclusion Olanzapine administration resulted in changes in central feeding regulation, gastrointestinal motility, thermogenesis and other physiological processes in mice, which might be involved in body weight gain induced by olanzapine. [ABSTRACT FROM AUTHOR]

Copyright of Cyber Security & Data Governance is the property of Editorial Office of Information Technology & Network Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Silk is the property of Zhejiang Sci-Tech University Magazines and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Yellow River is the property of Editorial Board of Yellow River and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the beneficial role and potential mechanism of intermittent fasting in olanzapine-induced metabolic disorders. Methods C57BL/6J mice were randomly divided into four groups: Saline + ad libitum (Saline+Ad libitum), Saline + intermittent fasting (Saline +IF), olanzapine administration + ad libitum (Olanzapine+ Ad libitum), and olanzapine administration + intermittent fasting (Olanzapine+IF)， with eight mice in each group. The IF group adopted the 5∶2 scheme, that is, fasting on Monday and Thursday every week, and eating freely in the rest of the time. Ad libitum feeding as the control of intermittent fasting, Saline gavage as the control of olanzapine administration. The experiment lasted for 12 weeks. The differences of body mass, liver mass and epididymal adipose tissue mass were compared between the olanzapine-treated group and the control group after IF intervention. The body fat mass, lean body mass, and visceral fat infiltration of mice were analyzed by nuclear magnetic resonance and HE staining, respectively. Furthermore, the levels of fasting blood glucose, insulin, and insulin resistance index (HOMA-IR) in the process of glucose metabolism were also measured by glucose oxidase method and radioimmunoassay, respectively. The effects of IF on H2O2 release and the level of cytochrome C mRNA, a marker related to mitochondrial damage, were detected by ELISA and real-time fluorescence quantitative PCR. Results After 12 weeks of treatment, olanzapine induced a significant increase in body mass, body fat, lean body mass and visceral fat infiltration (P<0.05), as well as fasting blood glucose, insulin, and HOMA-IR (P<0.05); however, IF significantly reduced the above indicators (P<0.05). Further studies showed that the release of H2O2 and the expression of Cytochrome C mRNA in adipose tissue of mice after intermittent fasting treatment were significantly decreased (P<0.05). Conclusion Intermittent fasting therapy can alleviate olanzapine-induced metabolic disorders in mice. The underlying mechanism may involve the inhibition of oxidative stress level and the maintenance of mitochondrial functions. [ABSTRACT FROM AUTHOR]

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Copyright of Science Technology & Engineering is the property of Chinese Society of Technology Economics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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