Search

Copyright of Journal of Xinyang Normal University Natural Science Edition is the property of Journal of Xinyang Normal University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Urban Mass Transit is the property of Urban Mass Transit Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shenyang Pharmaceutical University is the property of Shenyang Pharmaceutical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shenyang Pharmaceutical University is the property of Shenyang Pharmaceutical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electroplating & Finishing is the property of Electroplating & Finishing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Laser Technology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

With the advent of CRISPR technologies, almost at any specific locus in any genome of animal or plant, large-size DNA fragment can be inserted or replaced seamlessly following the occurrence of CRISPR nuclease-produced double-strand break (DSB) and donor DNA, and the induction of homology-directed repair (HDR) can be realized. At present, the homologous recombination (HR)-based CRISPR precision gene editing plays increasingly important roles in crop gene functional analysis and new techniques breeding. In this review, aiming at effective realization of HR-based CRISPR precision gene editing in plant cells, we briefly review the two HR-based mechanisms underlying CRISPR precision gene editing, i.e., synthesis-dependent strand annealing (SDSA) and non-homologous end joining (NHEJ) assisted single strand annealing (SSA). Based on this, we elaborate the DSB-producing and HDR-inducing CRISPR nucleases and DNA/RNA donors, mainly including Cas9/12 and fusion proteins, sgRNA/crRNA and modifiers, and donor DNA/ RNA and modifiers. Furthermore, we summarize the adopted delivery systems of CRISPR components and donor DNA/ RNA during plant genetic transformation, in light of keeping spatiotemporal consistency of DSB and donor DNA/RNA to improve HR efficiency. Finally, we provide an outlook for the promising applications in crop functional genomics study and new techniques breeding of HRbased CRISPR precision gene editing. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Irrigation & Drainage is the property of Journal of Irrigation & Drainage Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electroplating & Finishing is the property of Electroplating & Finishing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Evaluation & Analysis of Drug-Use in Hospitals of China is the property of Evaluation & Analysis of Drug-Use in Hospitals of China Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pharmaceutical Practice & Service is the property of Journal of Pharmaceutical Practice Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Research & Exploration in Laboratory is the property of Research & Exploration in Laboratory and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives] To provide technical supports for the detecting of myostatin gene (MSTN) knockout pigs and their derived food,a rapid multiplex PCR technique was established in this study. [Methods] Three pairs of specificity primers were designed according to the sequences of β2-microglobulin in pig, neomycin phosphotransferaseⅡ and the flanking sequences of the myostatin knockout pigs, respectively. The system of the multiplex PCR reaction was optimized by adjusting the amount of primers. The sensitivity of the technique was evaluated with transgenic pig samples. The bovine, sheep, mouse and untransgenic pig samples were used for analysing the specificity of the method. The spleen and lung samples of pig (n=32 )were collected, and tested using the method established. [Results] The optimized conditions were an annealing temperature at 56 ℃. The method could detect pig samples containing 1% MSTN knockout pig genome. There is only B2M amplified bands in test of 32 samples. [Conclusions] The results showed that the multiplex PCR technique established in the study had high sensitivity and specificity, in which only one reaction was necessary to detect multiple target fragments. It could be reliably used to identify with myostatin knockout pigs. This work lays a foundation for the establishment of detecting standard on the transgenic animals. [ABSTRACT FROM AUTHOR]

目的探讨2型糖尿病合并阻塞性睡眠呼吸暂停综合征(OSAS)患者的综合护理方法。方法选取该院 2015年1~12月收治的86例2型糖尿病合并OSAS患者实施综合护理,包括合理饮食、运动护理、曰常监测、并发症护 理、生活护理、健康教育及心理护理等,观察比较护理前后患者的血糖指标变化情况。结果护理后患者空腹血糖[(7.0± 0.5 )mmol/L]、餐后2小时血糖[(11.3 ±0.9)mmol/L]及糖化血红蛋白[(7.2±0.7) G ]水平均较护理前[(9.2 ±0.8 )& (13.3±1.3)mmol/L,(8.8±0.6)%]明显改善,差异均有统计学意义(!<0.05)。2型糖尿病合并OSAS与高血压、冠心病、糖 化血红蛋白水平升高及体质量指数增加有关,其中糖化血红蛋白水平升高是其独立风险因素。结论综合护理可明显改善 2型糖尿病合并OSHS患者的血糖水平。 [ABSTRACT FROM AUTHOR]