Objective To investigate HPV infection, subtype distribution, and age distribution in females in Kunming. Methods Flow fluorescence hybridization was used to detect HPV genotypes in 6 091 female cervical exserted cells in Kunming Maternal and Child Health Hospital in 2021. Infection rate, gene subtypes and age distribution were obtained after classification and statistics. Results 1 301 people wereinfected with HPV, the infection rate was 21.36%. Single infected persons accounted for the majority (78.94%). In all positive specimens, the number of high-risk infection cases was more than that of low-risk infection cases. The highest infection rates were HPV52 (21.83%), HPV16 (11.38%), and HPV58 (10.76%), respectively. Participants were divided into ≤ 20 years old group, 21 to 30 years old group, 31 to 40 years old group, 41 to 50 years old group, 51 to 60 years old group and > 60 years old group according to age. The HPV infection rate was highest in the > 60 years old group, followed by the 21 to 30 years old group and the 51 to 60 years old group. Conclusions HPV infection in women in Kunming is mainly a hhigh-risk type. Elderly and people under 30 years old need to strengthen prevention and treatment education, and it is recommended that women of appropriate age receive multivalent cervical cancer vaccine as soon as possible. [ABSTRACT FROM AUTHOR]