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Copyright of Journal of Chongqing University of Technology (Natural Science) is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Big Data is the property of Journal of Agricultural Big Data Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To prepare ultrasound contrast agent microbubbles combining anti-MUCl monoclone antibody and evaluate its targeting capability in vitro. Methods: Ultrasound contrast agent microbubbles and anti-MUCl monoclone antibodies were combined via N-succinimide 3-(2-pyridyl dithio) propionate (SPDP). Laser particle size analyzer, scanning electron microscope (SEM) and laser scanning confocal microscope (LSCM) were used to assess the diameter, morphology and ratio of combination between microbubbles and antibodies. Mice EMT6 breast cancer cells were cultured in vitro, which were then mixed with MUCl-targeted microbubbles. Adhension rate were evaluated via LSCM. Results: SEM showed MUCl-antibody carrying microbubbles were regular spherical particles with the diameter of 2.88 ± 1.34 u.m. Via SPDP, MUC1-antibodies were conjugated onto the surface of microbubbles with the ratio of 77.3 ± 10.4% by LSCM. Adhension rate was 79.2 ± 13.2%, when MUCl-antibody carrying microbubbles were mixed with Mice EMT6 breast cancer cells cultured in vitro. Conclusions: MUCl-antibody carrying microbubbles were prepared successfully, and could adhere to breast cancer cells in vitro. [ABSTRACT FROM AUTHOR]