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对于带地下室的高层及超高层结构,如何确定结构的嵌固端位置和考虑地基回填土的侧向约束作用是结构设计人员应关注的重要问题。本文首先提出了带地下室高层结构的力学模型,然后根据经典的弹性理论推导了结构的侧移及内力计算公式,并利用该公式分析了地基回填土约束作用与地下室高度对地下室底板反力M（0）和Q（0）、地下室顶板处位移y（h）以及结构顶点位移y（H）的影响。本文提出的公式以严密理论为基础,其计算结果可靠,可供科研人员和设计人员参考和应用。

[目的]观察绿色荧光蛋白（GFP）标记的恶性组织细胞增生症CY15细胞在Balb/c鼠眼前房生长及全身转移的规律,探讨GFP作为活细胞标记追踪肿瘤细胞生长、转移的优势并初步探讨肿瘤转移的机制.[方法]将携带绿色荧光蛋白的逆转录病毒MP71-GFP-PRE转入CY15细胞,G418筛选阳性克隆.20只Balb/c小鼠（20只右眼）,手术显微镜下每只小鼠眼前房接种2μL细胞密度为2.0×10^6个/mL的CY15GFP细胞悬液.术后15 d、20 d和30 d分别处死5只小鼠在荧光显微镜下观察全身转移情况.采用流式细胞仪分析术后30 d处死的另外4只小鼠和4只对照小鼠外周血单个核细胞中瘤细胞的比率.[结果]稳定表达绿色荧光蛋白的CY15GFP细胞基本保持了亲代细胞的特征,在前房的成瘤率为100%.术后15 d处死小鼠在荧光显微镜下观察可见肝、肺、胃肠壁等多个脏器中有散在的单个荧光点分布,为转移的单个肿瘤细胞,这些荧光点在肝脏中分布的数量最多.术后20 d、30 d分别处死5只小鼠,发现随着时间的推移这些微小转移灶逐渐增大.流式细胞仪分析术后30 d处死的小鼠外周血单个核细胞中瘤细胞的平均比率为0.048%±0.021%.[结论]绿色荧光蛋白标记瘤细胞后,利用荧光显微镜能有效地观察到肿瘤发生远距离转移在继发脏器中分布的单个瘤细胞和普通显微镜下无法分辨的微小转移灶,这些微小转移灶逐渐增大,为肿瘤转移的克隆起源学说提供了有力的证据.流式细胞仪能准确定量瘤细胞在外周血中的分布情况.

Objective To study the therapeutic effect of isopsoralen on bone loss induced by simulated microgravity in mice. Methods C57BL/6J mice were randomly divided into 5 groups; ground control group (CON), tail suspension group (HU), low dose group (ISO-L), middle dose group (ISO-M) and high dose group (ISO-H), with 12 mice in each group. After 21 days of administration, all mice were sacrificed, and the five internal organs, serum, femur and tibia were taken for histopathological examination, Mirco-CT imaging analysis, serum index detection, femoral bone tissue protein analysis and alkaline phosphatase positive clone staining. Results Compared with CON group, bone mineral density (BMD) and trabecular thickness (Tb. Th) in HU group decreased significantly (P<0.01), bone volume fraction (BS/TV), bone surface area density (BV/TV%) decreased significantly (P<0.05), trabecular number (Tb. N) decreased, trabecular separation (Tb. Sp) increased; Compared with HU group, BMD, BV/TV%, Tb. Th, BS/TV increased significantly (P<0.05), Tb. N increased and Tb. Sp decreased after tail suspension administration, and the effect of high dose group was the best. Compared with CON, serum osteocalcin (OCN) in HU decreased (P< 0.01), and the concentration of tartrate-resistant acid phosphatase 5b (TRACP 5b) increased (P<0.01); When isopsoralen was administered during tail suspension, OCN increased (P<0.01) and TRACP-5 decreased (P<0.01). Compared with CON group, OPG protein expression in HU group decreased (P<0.05) and RANKL protein expression increased ( P < 0.05 ) Compared with HU group, OPG and RANKL protein expression increased and RANKL protein expression decreased (P < 0.05) after isopsoralen administration. Compared with the ground control groupthe density of blue-purple alkaline phosphatase particles in HU group was lower and lighter, and the density of blue-purple alkaline phosphatase particles increased after isopsoralen was added during tail hanging. Conclusion Isopsoralen can promote bone formation and inhibit bone resorption under simulated microgravity, and effectively prevent bone loss. [ABSTRACT FROM AUTHOR]

Copyright of CT Theory & Applications is the property of Editorial Department of CT Theory & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Patients with chronic liver disease frequently exhibit thrombocytopenia, the etiology of which is intricate and not yet fully understood. As research into the association between chronic liver disease and thrombocytopenia has progressed in recent years, investigators have recognized that the thrombocytopenia in these patients is not solely attributed to splenic hyperfunction but is also modulated to a certain extent by thrombopoietin (TPO) . The article posits the possibility of a “liver-derived thrombopoietincentered gut-liver regulatory network mechanism”, providing a comprehensive review of the role of TPO in platelet regulation, the biological functions of platelets in patients with chronic liver disease, the diagnostic implications of TPO in chronic liver disease and cirrhosis, and the therapeutic applications of TPO and TPO receptor agonists in the treatment of thrombocytopenia in chronic liver disease. This review offers novel insights for both basic science and clinical research. [ABSTRACT FROM AUTHOR]

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Carbonate reservoirs in the Tahe oilfield are developed with fractures and vugs, drilling into vugs and lost circulation which in turn inducing blowout, borehole wall collapse and stuck pipe have frequently been encountered, and conventional bridging techniques cannot be used to effectively control the mud losses. To deal with the mud loss problem encountered in well workover and completion of old wells, a high temperature polymer gel lost circulation material (LCM) has been developed. Evaluation of the performance of the polymer gel LCM shows that before gelling, the viscosity of the polymer base fluid is in a range of 13 – 14.5 mPa∙s. At 150 ℃, the gelling time of the polymer can be adjusted between 30 min and 150 min. After gelling, the polymer gel loses its mobility and forms a high-strength gel plug. The polymer gel is tested on a 5 cm long iron-made core with 3 mm fractures, which simulates fractures found in the carbonate reservoirs, and a pressure bearing capacity of 2.1 MPa/42 cm is obtained. The polymer gel has good contamination resistance and good stability. The gel of the polymer can be easily broken, by adding alkaline solution into the gel to change the pH value, the gel becomes a low viscosity fluid in 48 h. After gel breaking, the permeability recovery of the rock cores that have been plugged with the gel can be greater than 85%. This high temperature polymer gel can be used to effectively plug formation pores and vugs, it can also be effectively removed away, thus making it a good choice for severe mud losses controlling in workover operations. [ABSTRACT FROM AUTHOR]

This paper presents a comprehensive chemistry experiment that translates a research result into undergraduate laboratory teaching. The experiment focuses on the synthesis of a highly sensitive electrochemical sensor for tert-butylhydroquinone (TBHQ) using ZIF-67 metal-organic framework (MOF) materials as a foundation. ZIF-67 MOF material was synthesized in solution at room temperature, followed by the preparation of Co nanoparticles and nitrogen-doped carbon nanotubes (Co/NCNTs) composite material through reduction atmosphere pyrolysis. The glass-carbon electrode was then modified with this material to investigate its detection capability for TBHQ, a common antioxidant component in edible oil. The morphology, structure, and elemental composition of the prepared materials were characterized using Scanning Electron Microscope (SEM), X-ray diffraction (XRD), Transmission Electron Microscope (TEM) and Energy Dispersive X-Ray Spectrometer (EDX). This experiment offers readily available reagents, simple operation, and a high success rate, providing students with comprehensive training in scientific research procedures, familiarity with the concept of metal-organic framework materials, and an understanding of structure-property relationships. It not only enhances students’ comprehensive laboratory skills but also cultivates their interest in scientific research and expands their perspectives. [ABSTRACT FROM AUTHOR]

Copyright of Current Biotechnology (2095-2341) is the property of Chinese Academy of Agricultural Sciences, Institute of Biotechnology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Traditional Chinese Drug Research & Clinical Pharmacology is the property of Traditional Chinese Drug Research & Clinical Pharmacology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: The type of graft selected during anterior cruciate ligament revision is considered one of the main factors affecting the postoperative outcome, but there are few reports on the comparison between different graft materials. OBJECTIVE: To explore the medium-to-long-term clinical efficacy after anterior cruciate ligament revision with autologous ligament, allogeneic ligament, and LARS artificial ligament. METHODS: A total of 67 patients with the first anterior cruciate ligament revision admitted to the Department of Joint and Sports Medicine, The Second Hospital of Tangshan from May 2011 to May 2020 were selected, including 41 males and 26 females, with a mean age of (45.83±7.39) years. They were divided into three groups according to different grafts used: autologous ligament group (n=24), allogeneic ligament group (n=22), and LARS artificial ligament group (n=21). Follow-up for more than 36 months after revision was performed to evaluate the effect of revision. RESULTS AND CONCLUSION: (1) International Knee Documentation Committee (IKDC) score, Lysholm knee score, and Tegner motor score 1 year after surgery and at the last follow-up in the three groups were higher than those before surgery (P < 0.05). There were no significant differences in IKDC score, Lysholm knee score, and Tegner motor score among the three groups 1 year after surgery and the last follow-up (P > 0.05). (2) The lateral differences of KT-1000 at 1 year after surgery and the last follow-up among the three groups were lower than those before surgery (P < 0.05). The lateral difference of KT-1000 and the positive rate of the axial shift test in the last follow-up of the LARS artificial ligament group were higher than those in the autologous ligament group and allogeneic ligament group (P < 0.05). (3) At the last follow-up, X-ray films showed no obvious enlargement of the reconstructed bone tunnel and no obvious failure of the graft fixation device. There was no obvious aggravation of osteoarthritis, but bone density decreased significantly in some elderly patients. (4) These findings suggest that anterior cruciate ligament revision with LARS artificial ligaments can obtain good initial stability, but with the extension of time, the stability of partial cases tends to decrease, even with reconstructed ligament failure. Both allogeneic and autogenous ligaments can obtain good clinical efficacy in anterior cruciate ligament revision. [ABSTRACT FROM AUTHOR]

Objective: To explore the molecular mechanism of miR-194-5p targeting down regulation of CD44 and inhibiting epithelial mesenchymal transition (EMT) in gastric cancer stem cells (CSCs). Methods: qRT-PCR was used to detect the expression levels of miR-194-5p, CD44, Snail, and membrane matrix metalloproteinase-1 (MT1-MMP) in gastric adenocarcinoma cell lines (SGC-7901, MGC-803, SPAG-9, and MNK-45) and gastric mucosal cells (GES-1). Western blot was used to detect the expression levels of CD44, Snail, and MT1-MMP proteins. miR-194-5p over-expression and low-expression plasmid vectors were constructed in vitro then to transfect with lentivirus. The experiment was divided into over-expression group, control group, and low-expression group. qRT-PCR and Western blot were used to detect changes of miR-194-5p, CD44, Snail, and MT1-MMP; CCK8 assay was used to detect cell proliferation rate, flow cytometry was used to detect cell apoptosis rate, Transwell assay was used to detect cell invasiveness, and Western blot was used to detect the expression levels of EMT markers E-cadherin and N-cadherin proteins. Results: The expression of miR-194-5p was significantly lower in gastric adenocarcinoma cell lines than gastric mucosal cells, while the expression levels of CD44, Snail, and MT1-MMP were significantly higher; what's more, the expression differences of SGC-7901 and MGC-803 compared to gastric mucosal cells were the most (P<0.05). Compared with the control group, miR-194-5p expression in the over-expression group was significantly more, but CD44, Snail, and MT1-MMP expressions were significantly less, cell proliferation and invasiveness were lower, apoptosis rate was higher, E-cadherin was upregulated, and N-cadherin was downregulated (P<0.05). Compared with the control group, the expression level of miR-194-5p was significantly lower in the low-expression group, while the expression levels of CD44, Snail, and MT1-MMP were significantly higher, the cell proliferation rate and invasiveness were more, while the apoptosis rate was less, E-cadherin was down-regulated, and N-cadherin was upregulated (P<0.05). Conclusion: Low expression of miR-194-5p in gastric cancer may exert an anti-tu-mor effect by downregulating the expression of CD44 in CSCs, thereby inhibiting the occurrence of EMT. [ABSTRACT FROM AUTHOR]

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Copyright of Fly Ash Comprehensive Utilization is the property of Hebei Fly Ash Comprehensive Utilization Magazine Co., Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Management & Technology Discussion is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The holding pole is a special lifting device for the construction of transmission towers. The optimization design model for the holding pole was established. The minimum mass was set as the optimization objective. The cross-section sizes of members, the connection modes of auxiliary members and the coordinates of the rocker joint were set the optimization variables. The allowable stress, displacement and buckling coefficient were taken as the constraining conditions. An improved sine cosine algorithm (ISCA) was proposed to carry out the size, shape and topology optimization designs of the holding pole. For the ISCA, the Lévy flight was introduced to enhance the global search ability, elite guidance strategy was applied to enhance the local search ability, and the greedy selection strategy was used to update the optimal solution. The example shows that, the ISCA can effectively solve the optimization design problems of spatial truss structures. [ABSTRACT FROM AUTHOR]

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OBJECTIVE: To probe into the clinical efficacy of Huanglian Wendan decoction in the treatment of ulcerative colitis of large intestine with damp-heat type. METHODS: Totally 60 patients with ulcerative colitis of large intestine with damp-heat type admitted into Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine from Oct. 2021 to Feb. 2023 were extracted to be divided into the control group and the observation group via the random number table method, with 30 cases in each group. The control group was treated with Mesalazine enteric-coated tablets alone, while the observation group received Mesalazine enteric-coated tablets combined with Huanglian Wendan decoction. The treatment course was 30 d. Changes of white blood cells (WBC), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), interleukin 6 ( IL-6) and other indicators of infection were compared before and after treatment in two groups. Colonoscopy Mayo score and ulcerative colitis endoscopic severity index ( UCEIS ) score were performed to record the disappearance time of diarrhea, bloody stool and abdominal pain during treatment and statistical analysis was performed. RESULTS: (1) After treatment, the overall effective rate of observation group was 83. 33% (25 / 30), significantly higher than that of control group 66. 67% ( 20 / 30), the difference was statistically significant ( P < 0. 05). ( 2) After treatment, the levels of WBC, CRP, ESR and IL-6 in both groups were significantly lower than those before treatment, with statistically significant difference (P<0. 05). There was no significant difference in infection indicators between two groups after treatment (P>0. 05). (3) The disappearance time of diarrhea, pus and blood stools, and abdominal pain in the control group was significantly longer than that in the observation group, the difference was statistically significant (P<0. 05). (4) After treatment, intestinal mucosal damage was significantly repaired in 25 patients in the observation group and 20 patients in the control group. Mayo and UCEIS scores in the observation group were significantly lower than those in the control group, with statistically significant difference (P<0. 05). CONCLUSIONS: Huanglian Wendan decoction can effectively improve the clinical symptoms of ulcerative colitis of large intestine with damp-heat type, promote intestinal mucosal repair, and improve the quality of life of patients, and has no obvious adverse drug reactions. [ABSTRACT FROM AUTHOR]

Endoplasmic reticulum (ER) is the predominant membrane structure in eukaryotic cells and is a key organelle for the occurrence of important intracellular physiological processes. Under the action of various internal and external factors, the homeostasis of ER is disrupted to block protein processing and transport, and the accumulation of unfolded or misfolded proteins in the ER lumen, forms endoplasmic reticulum stress (ERS) and triggers the unfolded protein response (UPR). Moderate ERS reduces protein synthesis, promotes protein degradation, and increases molecular chaperones that assist protein folding through the UPR signaling pathway, ultimately relieving ER stress. However, if the ERS is too strong or prolonged and exceeds the cell􀆳s ability to regulate itself, the UPR can initiate apoptosis and lead to diseases. Numerous studies have shown that ERS is closely associated with the development of several cardiovascular diseases (CVD). This review focuses on the research progress of UPR in several common types of CVD and targets UPR as a potential therapeutic approach for CVD. [ABSTRACT FROM AUTHOR]

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The purpose of the study was to study the effects of different levels of glucose oxidase on the egg laying performance, immunity and antioxidant capacity of Hyline brown laying hens in the late laying period. 1 000 healthy Hyline brown laying hens were selected and randomly divided into five groups, with 10 replicates per group and 20 hens per replicate. The Hyline brown laying hens in the control group, test group 1, test group 2, test group 3, and test group 4 were fed with 0, 100, 200, 400 and 800 mg/kg of glucose oxidase. The pre-test period was seven days, and the formal test period was 56 days. The results showed that the egg laying rate and average daily egg weight of Hyline brown laying hens in the test group 2, test group 3 and, test group 4 were significantly higher than those in the control group （P<0.05）, and the feed-to-egg ratio was significantly lower than that in the control group and the test group 1 （P<0.05）. The Huff units of eggs in the test group 3 and test group 4 were significantly higher than those in the control group and test group 1 （PcO.05）. The serum levels of immunoglobulin A and immunoglobulin M in the test group 3 and test group 4 were significantly higher than those in the control group and test group 1 （P<0.05）, and the serum levels of immunoglobulin G in the test group 2, test group 3, and test group 4 were significantly higher than those in the control group （JkO.05）. The serum levels of interferon-y, interleukin-2, and interleukin-10 in the test group 2, test group 3, and test group 4 were significantly higher than those in the control group （P<0.05）. The level of tumor necrosis factor-a was significantly lower than that of the control group and the test group 1 （Jk0.05）. The activities of total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in the serum of laying henS in血teSt gr°Up 2, test group 3 and test group 4 were significantly higher than those in the control group （P<0.05）, and the serum activities of superoxide dismutase in the test group 3 and test group 4 were significantly higher than those in the group 1 (P<0.05). The serum malondialdehyde level in all glucose oxidase groups was significantly lower than that in the control group (fk0.05). The study indicates that glucose oxidase can improve egg production performance, immunity, and antioxidant function of laying hens, and the appropriate addition level of glucose oxidase is 400 mg/kg.. [ABSTRACT FROM AUTHOR]

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Since the concept of dynamic covalent bonding was introduced, sulfur-containing dynamic polymers, as an important class of polymer in dynamic chemistry, have made breakthroughs in many fields such as chemistry, biology, and materials. The rapid development of sulfur-containing dynamic polymers is attributed to the various fascinating properties of elemental sulfur. Sulfur, as a common element in nature, is related to biological origins and essential to biological metabolism, such as sulfur in DNA and amino acids. Sulfur exists widely in nature, such as sulfur in crude oil, and sulfide ores in the geological environment. Sulfur-containing compounds are more active and diverse in physicochemical properties and participate in a variety of organic chemical reactions. In this review, different types of sulfur-containing dynamic covalent bonds (e.g., dynamic disulfide bonds, dynamic thioester bonds, dynamic thioacetal bonds, dynamic thiosemicarbazone bonds, and some unusual sulfur-containing dynamic bonds) are described. The effects of the reaction conditions and the chemical structure on the dynamic behaviors are briefly summarized. The mechanisms of dynamic behaviors of some sulfur-containing dynamic polymers are analyzed. In addition, the applications of various sulfur-containing dynamic covalent bonds to polymer design and applications in the fields of materials chemistry, electronics, and biomedicine are introduced. Finally, the current difficulties and challenges in the development of sulfur-containing dynamic polymer materials are analyzed, and an outlook on their future development is given. [ABSTRACT FROM AUTHOR]

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Ergothioneine (ERG), a rare natural sulfur-containing histidine derivative, has been proved to have strong antioxidant property and many biological functions. Therefore, ERG has been received much more attention from researchers and product developers. Currently, ERG has been widely used in food, cosmetics and medical industries. Research shows that ERG only can be synthesized by a few bacteria and fungi. Plants, animals and humans cannot synthesize ERG directly, but it could be obtained by other sources. ERG can be obtained by bioextraction, chemical synthesis, and biosynthesis. However, because of the low yield and poor production efficiency of the traditional methods (bioextraction and chemical synthesis), the large-scale production and application of ERG is limited. Therefore, there is an urgent need for an efficient, economical, safe and reliable ERG synthesis method to meet market needs. With the rapid development of synthetic biology, the use of genetic engineering, protein engineering and metabolic engineering to improve the ability of ERG biosynthesis has gradually become an increasingly favored method. This paper will elaborate the biological characteristics and functions, briefly introduce the biosynthetic pathways of ERG and application prospects of ERG in food, cosmetics and medical industries, compare the main production methods of ERG, and summarize and sort the research progress of adopting various engineering strategies to produce ERG by biosynthesis in recent years, and propose several engineering strategies on how to use genetic engineering, protein engineering and metabolic engineering to increase the yield of ERG, which aims to provide theoretical reference and research ideas for biosynthesis high-yield of ERG. [ABSTRACT FROM AUTHOR]