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Objective: To establish a PC-3M cell line stably expressing LBH gene and explore the effect of LBH gene on the proliferation of PC-3M cells. Methods: Recombinant lentiviral expression vector containing LBH gene was constructed and lentivirus was produced. After lentivirus infection of human prostatic carcinoma PC-3M cells, PC-3M-LBH cell line was obtained by screening with puromycin; Expression of LBH in different cells was examined by RT-PCR and Western blot; Cell proliferation was detected by CCK-8assay. Results: Lentiviral expression plasmid plenti-LBH was constructed and lentivirus particle was successfully packed. After infected with lentivirus and screened by puromycin, a cell line PC-3M-LBH was obtained. LBH expression in PC-3M-LBH was significantly higher compared with the NC control group and parental cells. The proliferation of PC-3M-LBH cells decreased 19.7 % compared with NC control and PC-3M at the 6th day after cells seeding. Conclusions: LBH gene expression can inhibit the proliferation of prostate cancer PC-3M cells. [ABSTRACT FROM AUTHOR]