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为更好地开发利用油橄榄叶，提高云南引种油橄榄的经济附加值，采用生化法及高效液相色谱法测定云南引种的16个品种油橄榄嫩叶中总黄酮、总酚含量及黄酮类和多酚类成分的含量，并对多酚类成分进行聚类分析，筛选不同用途优良茶叶品种。结果表明：16个品种油橄榄嫩叶中总黄酮含量为66.66~117.39 mg/g，总酚含量为35.63~55.81 mg/g，总黄酮和总酚含量均较高的5个品种为柯基、鄂植8号、豆果、城固32和尖叶木樨榄；16个品种油橄榄嫩叶的黄酮类成分为槲皮素、木犀草素、芹菜素、木犀草苷，含量分别为242.17~5 447.71 μg/g、818.48~2 707.34 μg/g、40.29~1 241.30 μg/g和1.35～5.17 mg/g，主要多酚类物质为橄榄苦苷（4.22～26.00 mg/g）、山楂酸（4.19~11.44 mg/g），其次为羟基酪醇（998.08~6 164.13 μg/g）、红景天苷（189.16~7 940.99 μg/g），其他多酚类成分含量较低。经聚类分析，16个品种油橄榄嫩叶分为5个亚类。柯基、鄂植8号、豆果3个品种油橄榄嫩叶中总黄酮、总酚及多酚类成分含量均较高，可作为抗氧化功能茶原料进行开发。In order to better exploit and utilize olive leaves and improve the economic added value of olive introduced to Yunnan, the contents of total flavonoids and total phenols，and contents of flavonoids and polyphenols components in the tender leaves of 16 varieties of olive introduced to Yunnan were determined by biochemical method and high performance liquid chromatography (HPLC), and the polyphenols components were also clustered and analyzed to screen the good varieties for tea.The results showed that the total flavonoids content in tender leaves of olive ranged from 66.66 mg/g to 117.39 mg/g, and the total phenols content ranged from 35.63 mg/g to 55.81 mg/g. The 5 varieties with high total flavonoids and total phenols contents were Keji, Ezhi 8, Douguo, Chenggu 32 and Olea cuspidate Wall. The flavonoids in 16 varieties of olive tender leaves were quercetin, luteolin, apigenin and luteoloside with contents of 242.17-5 447.71 μg/g, 818.48-2 707.34 μg/g, 40.29-1 241.30 μg/g and 1.35-5.17 mg/g, respectively, and the main polyphenols were oleuropein (4.22-26.00 mg/g) and maslinic acid (4.19-11.44 mg/g), followed by hydroxytyrosol (998.08-6 164.13 μg/g), rhodioloside (189.16-7 940.99 μg/g), and other polyphenols components with lower content. After cluster analysis, 16 varieties of olive tender leaves were divided into 5 subcategories. The tender leaves of 3 olive varieties Keji, Ezhi 8 and Douguo have higher total flavonoids, total phenols, and polyphenols components, which can be developed as raw materials for tea with antioxidant function.

为了对云南省引种油橄榄优异种质资源提供理论依据，收集云南省不同引种地不同品种的油橄榄果，用索氏法提取橄榄油，通过气相色谱法测定其脂肪酸组成及角鲨烯含量。结果表明：不同引种地同一品种的橄榄油脂肪酸组成具有一定的相似性，大多聚于一类；不同品种橄榄油具有不同的脂肪酸组成，同一品种不同成熟度油橄榄果制取的橄榄油脂肪酸组成差异不大；不同品种橄榄油中角鲨烯含量变化幅度较大，最低值与最高值之间相差8.2 mg/g。综上，同一品种橄榄油的脂肪酸组成总体上具有一定的遗传稳定性，不同品种橄榄油具有不同的脂肪酸组成，且角鲨烯含量差异显著。 In order to provide theoretical basis for the introduction of excellent germplasm resources of olive in Yunnan province, the oil was extracted by Soxhlet method from olive fruit with different varieties from different introduced areas in Yunnan province, and the fatty acid composition and squalene content of the oil were determined by gas chromatography. The results showed that the fatty acid composition of the olive oil from the same variety of olive fruit from different introduced areas was similar and mostly clustered in one group. The olive oil from different varieties of olive fruit had different fatty acid compositions, but the differences in the fatty acid composition of the olive oil from the same variety of olive fruit at different maturity levels were not significant. The squalene content of the olive oil obtained from different varieties varied considerably, and the difference between the lowest value and the highest value was 8.2 mg/g. In conclusion, the fatty acid composition of olive oils from the same variety of olive fruit is generally genetically stable, and the olive oil from different varieties of olive fruit has different fatty acid compositions and significant differences in the content of squalene.

为明确杂交油橄榄品种最佳采收期，以云杂1号、云杂2号和云杂3号为研究对象，于2018年6—10月期间观测果实表型性状、含油率及油质的变化，并采用主成分分析进行综合评价。结果表明：随着时间推移，3个品种横径、纵径、单果质量、果肉率、成熟度指数、含油率、亚油酸含量总体呈上升趋势，果形指数、含水量、油酸和亚麻酸含量变化趋势与之相反。不同采收时间各指标差异明显，9月末到10月末，3个品种果实生长趋于稳定，成熟度指数为4.56~5.52，果形指数在1.4左右，单果质量为1.25~1.41 g，果肉率为68.82%~71.94%，鲜果含水量为50.01%~51.53%、含油率为1610%~21.61%。从油质看，脂肪酸组成主要为油酸、亚油酸、棕榈酸、硬脂酸、亚麻酸和十六碳烯酸，占总量的98%以上，其中不饱和脂肪酸含量占82%以上。主成分综合评价结果显示，云杂1号最佳采收时间为9月末，云杂2号和云杂3号在10月末采收较为适宜。在最佳采收期综合性状最好的品种依次为云杂2号、云杂3号和云杂1号。In order to determine the best harvest time of hybrid olive varieties, three olive hybrid varieties Yunza No. 1, Yunza No. 2 and Yunza No. 3 were observed based on fruit phenotype characters, oil content and oil quality from June to October in 2018, and the principal component analysis was used for comprehensive evaluation. The results showed that with the delay of harvest time, the transverse diameter, longitudinal diameter, single fruit weight, pulp rate, maturity index, oil content and linoleic acid content of the three varieties showed an upward trend, while the change trends of fruit shape index, water content, oleic acid and linolenic acid content were opposite. There were significant differences in various indexes at different harvest time. From the end of September to the end of October, the fruit growth of the three varieties tended to be stable, the maturity index was 4.56-5.52, the fruit shape index was about 1.4, the single fruit weight was 1.25-1.41 g, the pulp rate was 6882%-7194%, the water content of fresh fruit was 5001%-51.53%, and the oil content of fresh fruit was 16.10%-21.61%. In terms of oil quality, the main fatty acid were oleic acid, linoleic acid, palmitic acid, stearic acid, linolenic acid and hexadecenoic acid, accounting for more than 98% of the total fatty acids, of which the unsaturated fatty acids content accounted for more than 82%. The principal component analysis showed that the best harvest time of Yunza No. 1 was the end of September, and Yunza No.2 and Yunza No.3 were more suitable to harvest at the end of October. The overall performance at the best harvest time decreased in the order: Yunza No. 2, Yunza No. 3 and Yunza No. 1.

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[Objective] To identify the members of the basic leucine zipper (bZIP) family of Rhododendron henanense subsp. lingbaoense genome, and to explore their expression patterns under abiotic stress. [Method] We identified the RhlbZIPs (bZIP TFs in R. henanense subsp. lingbaoense) using bioinformatic methods, and investigated their physical and chemical properties, gene structures, conserved motifs, evolutionary relationships, cis-acting elements, and expression patterns. In addition, real-time quantitative fluorescent PCR (RT-qPCR) was used to analyze the expression of RhlbZIP gene in abiotic stress. [Result] A total of 57 RhlbZIP genes were identified and divided into 11 subfamilies, and the same subfamily members having similar gene structure and conserved motifs. RhlbZIP genes were unevenly distributed in 13 chromosomes, and collinearity analysis revealed 45 replication events (3 pairs of tandem replications and 42 pairs of fragment replication). Cis-acting elements in the promoter region of RhlbZIP genes included four categories responding to transcription, development, abiotic or biological stress and hormone. GO annotation analysis showed that RhlbZIP gene family was associated with transcriptional regulation, metabolism, and biological processes. Transcriptome data showed that most bZIP family genes (82.46%) were expressed in 5 tissues of root, stem, leaf, flower, and hypocotyl, but there were significant differences in expressions. RT-qPCR analyses demonstrated that RhlbZIP4 was significantly up-regulated under low temperature treatment. RhlbZIP24 was significantly upregulated under drought and ABA treatment. RhlbZIP16 was significantly upregulated under high salt and MeJA treatment. [Conclusion] A total of 57 RhlbZIP genes have been identified in the R. henanense subsp. lingbaoense genome, and all selected genes are expressed under different treatments, and the major genes showing increase in expression in response to low temperature, drought, high salt, ABA and MeJA stress are RhlbZIP4, RhlbZIP24, RhlbZIP16, RhlbZIP24 and RhlbZIP16, respectively. [ABSTRACT FROM AUTHOR]

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BACKGROUND: Atlantoaxial dislocation, because of its high difficulty and high risk of surgery, has been regarded as the “surgical restricted area” by the international orthopedic community. However, with the rapid development of intelligent digitization in orthopedics, robot-assisted navigation screw placement technology has been widely used in clinical practice, which significantly reduces the difficulty and risk of surgery and improves the safety of surgery. However, there are few reports on the application of this technique in the treatment of atlantoaxial dislocation. OBJECTIVE: To explore the application value of robot-assisted pedicle screw internal fixation in the treatment of atlantoaxial dislocation. METHODS: The medical records of five patients with atlantoaxial dislocation treated with C1-C2 pedicle screw fixation under robot-assisted navigation in Zhuhai Hospital of Guangdong Provincial Hospital of Chinese Medicine from October 2021 to July 2022 were retrospectively analyzed. Operation time, length of neck incision, blood loss, postoperative drainage volume, and length of hospital stay were recorded. Attention should be paid to cerebrospinal fluid leakage, vertebral artery injury, nerve injury, operative area infection and other complications. The visual analog scale score of neck pain, the spinal cord injury grade of the American Spinal Injury Association, the cervical spine score of the Japanese Orthopaedic Association, and the imaging indicators were collected before surgery and at the last follow-up. Screw placement accuracy was assessed. RESULTS AND CONCLUSION: (1) Five patients were successfully completed surgery, without vascular, nerve injury or other complications, and were followed up for 12-20 months. (2) A total of 20 cervical pedicle screws were placed in 5 patients, including 9 type A screws, 10 type B screws, and 1 type C screw. The accuracy of screw placement was 95%. (3) At the last follow-up, the visual analog scale score was (0.80±0.71) points, which was significantly lower than that before operation (4.00±2.83) points; the Japanese Orthopaedic Association score was (14.80±0.84) points, which was significantly higher than that before operation (8.00±0.71) points. Anterior atlantodental interval decreased from (7.86±3.25) mm to (2.82±0.93) mm; space available of the spinal cord increased from (6.74±1.99) mm to (12.10±3.51) mm; cervicomedullary angle increased from (133.32±13.55)° to (153.44±9.53)°; clivus-canal angle increased from (128.02±9.92)° to (143.25±12.99)°. The results of the last follow-up indexes were improved compared with those before operation, and the differences were significant (all P < 0.05). (4) Postoperative imaging follow-up showed that all patients had bone fusion in the bone graft area, and no internal fixation loosening, fracture or pull-out occurred. (5) This method can avoid relying on the doctor’s experience and hand feeling, ensure the accuracy of upper cervical screw placement, reduce the risk of surgery, and obtain satisfactory results in mid-term follow-up. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this paper, type-Ib diamond single crystals, using two different sizes of synthesis cavities of ϕ15 mm and ϕ30 mm, were synthesized under 5. 7 GPa, 1 560 ~ 1 600 K, and the temperature field distributions of the two sizes of synthesis cavities were studied using finite element method (FEM). Firstly, the temperature field distributions of two different sizes of synthesis cavities were simulated using finite element method. The average radial temperature gradient and average axial temperature gradient of the ϕ15 mm synthesis cavity are 0. 573 and 5. 700 K/mm, respectively. The average radial temperature gradient and average axial temperature gradient of the ϕ30 mm cavity are 0. 093 and 2. 280 K/mm, respectively. The simulation results reveal that the uniformity of the temperature field in the ϕ30 mm synthesis cavity is significantly better than that in the ϕ15 mm synthesis cavity. Secondly, the reproducible growth of high-quality type-Ib diamond large single crystals was achieved using both synthesis cavities mentioned above. The ϕ15 mm synthetic cavity is difficult to achieve the growth of high-quality type-Ib diamond single crystals weighing more than 1. 2 ct (1 ct =0. 2 g), while the ϕ30 mm synthetic cavity is more suitable for the growth of large-size high-quality type-Ib diamond single crystals. Furthermore, the scanning electron microscopy (SEM) test results show that the surface flatness of high-quality type-Ib diamond single crystals grown using two different sizes of synthesis cavities in this study is good, and the expansion of the synthesis cavity has no significant effect on the crystal quality of Ib diamond single crystal surface. Finally, Raman spectrum test results show that the crystal quality of polycrystalline diamond deteriorates, and other diamond single crystal samples synthesized by two kinds of synthesis cavity in this study have better crystal quality. This research has certain academic reference value for the design of large size synthesis cavity of gem grade diamond single crystal, the growth of large size diamond single crystal, and the perfection of polycrystalline diamond single crystal synthesis technology. [ABSTRACT FROM AUTHOR]

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In this study, the impact of climate change on the agronomic traits of winter wheat in Shangluo dryland in recent years was researched, aiming to provide theoretical guidance for the breeding for new varieties of Shangluo dryland winter wheat that adapts to climate change. Using the agronomic traits data of wheat variety Xiaoyan15 at the Shangzhou site of the wheat regional trial in the Shangluo sub-region of the hilly areas of southern Shaanxi from 2010 to 2023 and meteorological data of Shangluo, the trend of wheat agronomic traits and climate change were statistically analyzed, and correlation, multiple regression, and path analysis were conducted. The results showed that the annual precipitation and average temperature of Shangluo, as well as the precipitation and temperature during the wheat growth period in Shangluo, had all shown an upward trend in recent years. At different growth stages of wheat, the precipitation from sowing to overwintering, overwintering, and from turning green to booting all showed an upward trend, while the precipitation from heading to maturity showed a downward trend. The temperature at each growth stage of wheat showed an upward trend. The agronomic traits of wheat, such as spike length, grains per spike, thousand grain weight, effective spike number, and yield, all showed an upward trend, while the growth period and plant height showed a downward trend. Correlation analysis found that the average temperature during the whole growth period had extremely significantly positive correlation with spike length, significantly positive correlation with grains per spike and yield, and extremely significantly negative correlation with growth period. The temperature from turning green to booting had significantly negative correlation with the growth period. The precipitation during overwintering had significantly positive correlation with spike length. And there was an extremely significantly positive correlation between yield and spike length and grains per spike. Multiple regression and path analysis revealed that spike length, thousand grain weight, grains per spike, and average temperature during the whole growth period were the four main factors determining yield, collectively determining 90.80% of the variation in yield. Based on the above analysis, in order to adapt to climate change in the Shangluo region, the breeding process of new varieties of dryland wheat should focus on the traits such as spike length, grains per spike, and growth period. [ABSTRACT FROM AUTHOR]

To address the low utilization of raw data and insufficient feature extraction capability of multi-dimensional data in existing remaining useful life prediction methods, this paper proposed a convolutional neural network model based on feature enhancement and spatio-temporal information embedding. Firstly, it adopted a feature enhancement module to extract additional operating condition features and manual features from raw data as auxiliary features. Then, it introduced the spatio-temporal embedding module to encode the spatio-temporal information, embedding the time series information and spatial feature information into the original data. Finally, it concatenated the aforementioned features, and it employed a regression prediction module to capture the inherent relationships in the data and obtain regression prediction results. It evaluated the predictive effectiveness of the proposed model on the commonly used commercial modular aero-propulsion system simulation(C-MAPSS) dataset. The experimental results show that the root mean square error of the proposed model decreases by 8.8% on average over the four subsets compared with other mainstream deep learning methods, and it also outperforms existing state-of-the-art algorithms in prediction accuracy under multiple operating conditions and fault types. The experiments fully verify the effectiveness and accuracy of the proposed model in predicting the remaining useful life of turbofan engines. [ABSTRACT FROM AUTHOR]

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本文深入探讨了医疗设备的质量控制措施与标准化管理体系建设的重要性、核心内容和实施策略。医疗设 备的质量控制是确保医疗安全、提高医疗效率的关键环节, 而标准化管理体系的建设则为医疗设备管理提供了系统性的 框架。本文旨在为医疗行业的设备管理提供理论支持和实践指导, 以促进医疗设备管理的规范化、科学化和高效化。 [ABSTRACT FROM AUTHOR]

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Annexin (ANX) are a family of intracellular proteins that bind membrane phospholipids in a calcium ion(Ca2+)-concentration-dependent manner and are widely expressed in various cell types.ANXA11 is a member of the Ca2+-regulated phospholipid-dependent and membrane-bound ANX multigene family. Previous studies have shown that ANXA11 has important roles in cell division, Ca2+ signaling, vesicle trafficking, and apoptosis. Recent studies have shown that dysregulation and mutation of ANXA11 are associated with the development of systemic autoimmune diseases and cancers, chemotherapy resistance, and recurrence, etc.ANXA11,which is highly expressed in a variety of tumors, can be involved in tumorigenesis and development as a pro-carcinogenic gene, and is expected to be a potential molecular marker for cancer diagnosis, treatment, and prognosis. This article reviews the biological function and the research progress of ANXA11 in tumors in recent years.  [ABSTRACT FROM AUTHOR]

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