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为筛选可高效降解菜籽饼粕中硫苷的细菌，给硫苷降解基因工程菌的构建提供实验数据，以堆放菜籽饼粕的土壤为分离源，通过硫苷选择培养基筛选及硫苷降解率测定，获得一株硫苷降解率最大的菌株C1，采用16S rRNA基因序列进行种属鉴定，通过第3代Illumina NovaSeq PE150测序，获得其基因组完成图并进行功能注释，借助比较基因组学探究C1降解硫苷的机制。结果表明：菌株C1为枯草芽孢杆菌 (Bacillus subtilis)，其硫苷降解率为 (68.98±4.74)%；C1菌株的基因组染色体全长为4 139 381 bp，鸟嘌呤和胞嘧啶含量为43.88%，编码区总长度占全基因组的比例为89.14%；C1菌株能编码大量的功能蛋白，主要涉及氨基酸转运与代谢；比较基因组学分析确认，C1与枯草芽孢杆菌的模式菌株 (NC000964.3) 共线性高，序列中未见大片段易位和倒置的区域，但C1序列中2.7 Mb处出现一个小片段的插入，2.1~2.4 Mb处出现小片段缺失，分别涵盖了2条黑芥子酶的相关基因，推测C1的硫苷降解机制为其可高效分泌黑芥子酶。C1菌株能够高效降解菜籽饼粕中的硫苷，是构建硫苷降解基因工程菌的可选材料。 In order to screen bacteria that can efficiently degrade glucosinolates in rapeseed meal and provide experimental data for the construction of glucosinolate-degrading genetically engineered bacteria, the soil stacked with rapeseed meal was used as the separation source, and a strain C1 with the greatest glucosinolate degradation rate was obtained by glucosinolate selection medium screening and glucosinolate degradation rate determination,and 16S rRNA gene sequence was used for C1 species identification and the third generation Illumina NovaSeq PE150 was used to sequencing the whole genome to obtain C1 genome completion map and functional annotation was carried out. The comparative genomics was used to explore the mechanism for glucosinolate degradation of strain C1. The results showed that strain C1 was Bacillus subtilis, and its glucosinolate degradation rate was (68.98±474)%. The genome chromosome of the strain C1 was 4 139 381 bp in length, and the guanine and cytosine content was 43.88%. The total length of the coding region accounted for 89.14% of the whole genome. Strain C1 could encode a large number of functional proteins, mainly related to amino acid transport and metabolism. Comparative genomics analysis confirmed that C1 was highly colinear with the model strain of Bacillus subtilis (NC000964.3), and there were no large fragment translocation and inversion regions in the sequence. However, a small fragment insertion was found at 2.7 Mb in C1 sequence, and a small fragment deletion appeared at 2.1-2.4 Mb,covering two gens of myrosinase, respectively, and the mechanism of glucosinolate degradation of C1 was presumed to efficiently secrete myrosinase. Strain C1 can efficiently degrade glucosinolates in rapeseed meal and is a potential material for constructing glucosinolate-degrading genetically engineered bacteria.

[Objective]To evaluate the flammability of branches and leaves of common tree species in Sichuan Province. [Method] Seventy common ecological/ timber/ economic tree species were selected. Ignition point, heat value, ash content, crude fat content, and moisture content of branches and leaves were measured. Principal component analysis was used to evaluate the flammability of tree species comprehensively. [Result]Flammability varied significantly across different species. High flammable species include Pinus tabuliformis, Pinus bungeana, etc., low flammable species include Mangifera indica and Malus spectabilis, etc. [Conclusion]This study provides data support for forest fire prevention in Sichuan Province, and tree species with low flammability can be used as potential fire-resistant species. [ABSTRACT FROM AUTHOR]

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Blood lipids are crucial for maintaining normal physiological homeostasis, and their levels are closely related to the occurrence, development, and metastasis of cardiovascular diseases and cancer. Statins are commonly used drugs to regulate blood lipids, resist inflammation, protect vascular endothelium, and prevent platelet aggregation. Their anti-tumor effects and effects are also receiving widespread attention. The article reviews the relationship between blood lipid levels and four common tumors, as well as the mechanisms of statin drugs in regulating blood lipid levels and anti-tumor effects. Statins have the potential to play a greater role as tumor drugs in tumor treatment, while also regulating blood lipid levels. [ABSTRACT FROM AUTHOR]

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LTE-A（增强型长期演进）系统上行引入了多天线传输技术来解决终端天线较少的问题。在这种技术背景下,设计了一种上行传输模式自适应方法以提高系统性能。从传输模式间自适应、单双流自适应和MU-MIMO（多用户多输入多输出）与单天线传输之间的自适应三个方面进行了论述。系统级仿真表明,基站根据信道条件的变化选择适当的传输方式可以有效地降低用户误码率,提高系统吞吐量。

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