To investigate the physiological response characteristics of cucumber to iso-osmotic NaCl and Ca(NO3)2 stress and the differential regulation mechanism of exogenous putrescine, so as to provide a theoretical basis for applying putrescine to alleviate soil salinization and conduct stress resistant cultivation of cucumber. In this study, the effects of foliar spraying exogenous sapropamine(8 mmol/L) on the growth and physiological characteristics of cucumber seedlings under iso-osmotic salt stress were studied using Jinchun 3 cucumber as test material, using 75 mmol/L of NaCl and 50 mmol/L of Ca(NO3)2 to simulate the conditions of iso-osmotic salt stress. The results showed that under iso-osmotic salt stress, exogenous putrescine induced plant antioxidant response by increasing leaf relative water content and chlorophyll content, mitigated membrane lipid peroxidation damage to protect membrane integrity, improved cell osmotic potential and regulated the synthesis of endogenous osmotic substances, thus enhancing cucumber seedling tolerance to iso-osmotic salt stress. Compared with simple salt stress, exogenous putroamine induced significant increase of POD activity in cucumber seedling leaves and roots under iso-osmotic salt stress. The activity of superoxide dismutase(SOD) in leaves increased significantly. Under NaCl stress, catalase(CAT) activity in leaves significantly increased, and CAT activity in roots significantly decreased. However, under Ca(NO3)2 stress, leaf CAT activity decreased significantly, and root CAT activity increased significantly. Meanwhile, exogenous putroamine induced significant increase of osmotic potential and the contents of free amino acids and soluble protein in seedling leaves and roots under NaCl stress, the content of proline in leaves significantly increased. Under Ca(NO3)2 stress, the contents of free amino acids and soluble protein in leaves significantly increased, and osmotic potential and the contents of soluble protein and soluble sugar in roots significantly increased. Exogenous putrescine could significantly alleviate iso-osmotic NaCl and Ca(NO3)2 stress, but there were differences for exogenous putrescine to alleviate the two salt stresses in antioxidation and osmotic regulation mechanisms in cucumber seedlings. [ABSTRACT FROM AUTHOR]