Objective: To explore the distribution features and relationship between HR-HPV genotypes in Jiangsu women and normal cervix and precancerous lesions (CIN I, CINII, CIN III) and cervical cancer, thus providing theoretical basis for prevention of cervical cancer. Methods: 424 Jiangsu women underwent pathological examination of cervical tissues from December 2014 to December 2015 in our hospital were analyzed by HPV typing detection. They were divided into normal group, CIN I group, CIN II group, CIN III group and cervical cancer group according to the pathological examination results. Chi-square test was applied to study the relationship between HR-HPV infection of Jiangsu women and different cervical lesion groups. Results: The prevalence of high-risk HPV infection in normal group, CIN I group, CIN II group, CIN III group and cervical cancer group were 24.3 %, 50.0 %, 71.5 %, 75.9 % and 82.1 %, respectively. There was statistically difference in high-risk HPV prevalence between normal group and four cervical lesion groups (P< 0.01). There was no difference between the prevalence of single infection and multiple infection (P=0.973); the distribution of HR-HPV types in cervical lesion groups differed with the aggravation of the degree of cervical lesions. In CIN I, the common genotypes were HPV 16, 52, 58 and 51. In CIN II/III group, the most common genotypes were HPV 16, 58, 52, 33 and 31. In cervical cancer group, the most common genotypes were HPV 16, 53, 18 and 58. The prevalence of high-risk HPV was found to be highest at the age over 49 years old, and to be lowest at the age of 20-29 (P<0.01). Conclusions: The severity of cervical lesions in Jiangsu women was positively correlated with the prevalence of HR- HPV infection, but it was negatively correlated with the number of high-risk HPV subtypes infection. Besides HPV16, HPV58, 52, 33 and 31 were all closely related with cervical cancer and precancerous lesions. The prevalence of high-risk HPV had age differences, Jiangsu women over 49 years old should be paid more attention, HPV genotyping detection and cervical lesions screening should be done termly. [ABSTRACT FROM AUTHOR]