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Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives] This paper was aimed to confirm the interaction between bip130(BR11-interacting protein 130) and OSA7(plasma membrane H+-ATPase 7) and verify that bip130 could regulate the activity of plasma membrane H+-ATPase in rice roots under ABA treatment. [Methods] Yeast two-hybrid technology(Y2H),firefly luciferase complementary imaging system(LCI),GST pull-down and bimolecular fluorescence complementary technology(BiFC) were used to verify whether bip130 interacts with OSA7. Then,Y2H and LCI had been used to identify the interaction region between bip130 and OSA7 and the relationship between bip130 and OSA7 homologous proteins. RT-qPCR had been used to analyze the upstream and downstream relationship between bip130 and OSA7 under ABA treatment. The bip130 mutant materials have been used in the rice protoplast transient expression system to detect whether bip130 could affect the activity of plasma membrane H+-ATPase. [Results] The results indicated that bip130 interacted with the cytoplasmic N-domain of OSA7,but did not interact with the cytoplasmic N-domain of these five homologous proteins. In the ABA signaling pathway,bip130 was located in the upstream of OSA7. Mutant analysis showed that bip130 overexpression could further enhance the activity of ABA-induced plasma membrane H+-ATPase,while bip130-RNAi prevented this process. The analysis of the protoplast transient expression system indicated that bip130 could regulate the activity of plasma membrane H+-ATPase in the ABA signaling pathway. [Conclusions] bip130 interacted with the cytoplasmic N-domain of OSA7 and bip130 participated in the regulation of plasma membrane H+-ATPase activity in ABA signaling pathway. [ABSTRACT FROM AUTHOR]