Carnivores play a key role in maintaining the stability of food webs and the balance of ecosystem functions, and understanding the temporal niches between predators and their prey can contribute to the rare species conservation. During September 2018 to August 2020, we deployed 60 camera traps to monitor seasonal variation in the daily activity rhythm and overlap patterns of leopard cats (Prionailurus bengalensis) with their potential prey in the Xinglong Mountains National Nature Reserve of Gansu Province. We identified a total of 370 independent effective events for leopard cats, 390 for woolly hare(Lepus oiostolus), 760 for rats and 834 for common pheasant(Phasianus colchicus)over 33, 521 camera trapping days. Kernel density estimation and coefficient of overlap analyses showed that：(1)The daily activity rhythms of leopard cats and rats were bimodal nocturnal, while woolly hare mainly occurred at night and common pheasant were bimodal diurnal.(2)Leopard cats and their major prey (except for common pheasant) had obvious seasonal variation, which all tended to be active in the early morning during the hay period, and the leopard cat also increased its activity hours in the afternoon with flexible adaptability. (3)Leopard cats had a higher overlap coefficient with rats during the grass period(∆4=0. 779)and with woolly hare during the hay period(∆1=0. 720), but had the lowest overlap with common pheasant during both grass period (∆4=0. 356)and hay period(∆1=0. 453).This study is useful for evaluation the response of leopard cats as top predators to their potential prey in the temporal dimension, and provide scientific basis for the conservation of leopard cat and predator-prey coexistence in the Xinglong Mountains. [ABSTRACT FROM AUTHOR]