In the experiment, different concentrations of N-carbamylglutamic (NCG) were added to the diet to determine the production performance, egg quality, plasma nitric oxide (NO) and nitric oxide synthase ( NOS) level changes, to explore the effect of NCG-NO pathway on egg production and egg quality functions of Dagu chicken hens. A total of 12-week-old 192 Dagu chicken hens were randomly divided into four groups with four replicates in each group and 12 replicates in each group. The control group Dagu chicken hens were fed with basic diet, and the experimental groups Dagu chicken hens were fed with 0.08% and 0.12%, 0.16% level of NCG diet. The pre-test period was 1 w, and the experiment lasted for 25 w. The results showed that the egg production rate of 0.08% NCG group and 0.12% NCG group was significantly higher than that of control group (P<0.05). Among all groups, 0.12% NCG group had the lowest feed to egg ratio, and yolk color in NCG groups was significantly higher than that in control group (P<0.05). The egg shape index and the protein height of the 0.12% NCG group were significantly higher than that of control group and other experimental groups (P<0.05). NO content of 0.12% NCG group was significantly higher than other groups (P<0.05). The experiment indicates that dietary supplementation of 0.08% to 0.16% NCG can improve performance and egg quality of broilers during peak laying period, and the effect of NCG at 0.12% NCG level is the best. [ABSTRACT FROM AUTHOR]