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目的比较近距离用眼前后黄斑区浅层视网膜微循环指标的变化，探讨近距离用眼影响近视的可能原因。方法采用近距离观看手机视频1 h作为近距离用眼的方式，通过光学相干断层扫描血管成像（OCTA）技术测量用眼前、后黄斑区6 mm × 6 mm范围内的浅层视网膜微循环指标，包括浅层（神经纤维层至外丛状层内界）视网膜血管长度密度（VLD）、血管灌注密度（VPD）以及黄斑中心凹无灌注区（FAZ）的面积（FAZ-A）、周长（FAZ-P）、形态指数（FAZ-CI），按区域划分为中心、内层、外层及整体，得出11项微循环指标，对比用眼前及用眼后各项指标的差异。结果近距离用眼后的黄斑区浅层视网膜微循环指标普遍较用眼前低，除了FAZ-A及FAZ-P以外（P = 0.148、P = 0.975），差异均具有统计学意义，VLD中以中心VLD（差异1.00）及内层VLD（差异0.80）的差异最大（P = 0.001、P = 0.008），VPD中以中心VPD（差异0.02）及内层VPD（差异0.02）最大（P = 0.001、P = 0.008），不同屈光度及眼轴的青年变化程度无差异。结论近距离用眼可能通过影响黄斑区浅层视网膜微循环的改变，导致局部缺血缺氧，影响近视的发生及发展。

【目的】探讨血小板源性生长因子-C（PDGF-C）蛋白治疗对视网膜光感受器细胞损伤模型中的神经保护作用。【方法】①检测体外条件下PDGF-C的作用，使用光感受器细胞系661W细胞及原代培养的Pde6brd1 视网膜细胞。在体外培养的661W细胞培养液中加入PDGF-C蛋白或者牛血清蛋白（BSA），分别予以同等强度相同时间长短的紫外线照射，然后做MTT 检测其细胞凋亡状况，检测PDGF-C 蛋白对661W细胞的保护作用。取Pde6brd1小鼠视网膜做原代细胞培养后，在培养液中分别加入PDGF-C蛋白或BSA，相同条件培养后通过免疫荧光染色检测光感受器细胞的生存情况，以检测PDGF-C 蛋白对该原代细胞的保护作用。②检测在体条件下PDGF-C的作用，在Pde6brd1小鼠的视网膜下分别注射PDGF-C蛋白及BSA，然后通过组织切片后苏木精-伊红（HE）染色观察视网膜光感受器细胞数量的变化来检测PDGF-C的神经保护作用。【结果】在紫外线诱导的视网膜光感受器细胞661W 损伤模型中，培养液中加入PDGF-C 蛋白的实验组中，MTT 实验显示490 nm 吸光度（0.2857 ± 0.054）较对照组（0.1726 ± 0.005）增高，P < 0.05。在Pde6brd1原代培养的视网膜细胞中，PDGF-C蛋白组中光感受器细胞的存活率明显高于对照组，P < 0.05。在Pde6brd1小鼠的视网膜下注射PDGF-C蛋白，实验组外核层中的每100 μm细胞计数（38.4769 ± 6.1）较对照组（27.2435 ± 5.3）增加，P < 0.05。【结论】PDGF-C蛋白在光感受器细胞损伤模型中具有较强的神经保护作用。

目的 对比观察锁骨上臂丛神经阻滞术完成即刻静脉注射 5, 10 mg地塞米松的上肢骨折手术患者反跳 痛发生情况, 为地塞米松更优剂量的选择提供参考依据. 方法 将 116 例上肢骨折患者随机分为 D1 组, D2 组, C 组, 分别为39, 39, 38例. 患者入室后均使用0.3%罗哌卡因30 mL完成超声引导下锁骨上臂丛神经阻滞. D1组在 完成阻滞后静脉注射地塞米松5 mg, D2组静脉注射地塞米松10 mg, C组静脉注射生理盐水5 mL. 比较三组神经阻 滞后24 h内反跳痛的发生率, 反跳痛发生时间, 反跳痛持续时间和最严重疼痛强度［疼痛数字评分 (NRS)], 神经阻 滞持续时间, 术前及术后8, 12, 24, 48 h静息时NRS评分, 术后48 h内镇痛泵自控按压次数，补救性镇痛概率以及并 发症发生率. 结果 与C组比较, D1组, D2组反跳痛发生率低, 反跳痛发生时间延迟 (P均<0. 05). D1组, D2组反 跳痛发生率, 发生时间比较, P均>0. 05. 三组反跳痛持续时间和最严重疼痛强度比较，P均>0. 05. 与C组相比, D1 组, D2组神经阻滞作用持续时间均延长, 且术后 24 h的 NRS评分低，补救性镇痛概率低（P均<0. 05). 三组不良反 应发率比较, P均>0. 05. 结论 5 mg和10 mg地塞米松静脉注射对上肢骨折手术患者锁骨上臂丛神经阻滞后反跳 痛均有预防作用, 可减少神经阻滞后反跳痛发生, 延缓反跳痛的发生时间, 降低术后24 h的疼痛程度, 减少术后补救 镇痛次数, 临床推荐地塞米松应用剂量为5 mg. [ABSTRACT FROM AUTHOR]

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鼻咽粘膜上皮细胞如何癌变、异型增生及鳞状化生与癌变的关系如何,目前仍不十分清楚,本文从形态发生的角度探讨其相互关系。材料和方法鼻咽活检1049例,全部均存有粘膜上皮,无上皮者不包括在内。按活检有无癌组织分成癌组(783例)和非瘤组(266例)。切片常规染色,观察粘膜上皮的类型、增生、化生及早期癌变的情况。

本文报告27例肺癌的角蛋白—爱新蓝染色结果并用电镜观察对照。角蛋白染色阳性率为92％,张力原纤维发现率89％。中～强度染色者鳞癌100％,腺癌27％。有助于判断肿瘤组织来源,辅助肿瘤分类,了解肿瘤浸润情况。

本文收集了94例慢性萎缩性胃炎病者的胃粘膜标本,从病理组化角度观察维酶素对萎缩和肠化生上皮的作用。 患者分为治疗组与对照组:前者口服维酶素每次1克,每日3次,共6～16周;后者口服谷维素,每次20mg,每日3次,疗程相同。观察结果表明维酶素对萎缩的和肠化生的胃粘膜上皮有明显的改善,两者的有效率分别为67％和85％。相比之下,对照组的有效率仪为18.7％和54％。结果提示维酶素可能有抑制萎缩性胃炎的粘膜上皮肠型化生,以及刺激上皮增生的作用。

正常鼻咽粘膜大部分由假复层纤毛柱状上皮披覆,只有小部分才是由鳞状上皮复盖。但是鼻咽癌却绝大多数属于鳞状细胞癌。鼻咽粘膜的假复层纤毛柱状上皮怎样转变成癌很值得研究。关于鼻咽癌的组织发生学以前有过一些报道。本文通过癌巢近旁鼻咽粘膜和非癌鼻咽粘膜上皮病变的观察,对鼻咽粘膜上皮癌变过程作进一步的探讨。

本文报告一例典型的肺硬化性血管瘤,女性,37岁,左下肺双发性肿瘤。基于细胞学,组织学、免疫组化及电镜的观察,确证肿瘤有向上皮性和纤维性双相分化的能力,认为是一种来源于间皮的肿瘤。

鼻咽恶性肿瘤病理组织学类型的构成比在高发区、中发区和低发区有所不同。这主要是指成人。本文研究的主要是儿童鼻咽恶性肿瘤的病理形态特点,探索儿童鼻咽恶性肿瘤病理组织学类型的构成比在高发区、中发区和低发区有无不同,以及儿童和成人鼻咽恶性肿瘤病理形态改变的差异。

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Stephania kwangsiensis, a vine plant of the Stephaniaceae family, is mainly spread in Guangxi, China. The medicinal part(root tuber)of S. kwangsiensis, "Jin Bu Huan" in Chinese, is often used for sedative, insecticidal, anti inflammatory, anti-tumor, antiviral and antibacterial, as well as for the treatment of Alzheimer's disease. In order to make full use of the plant resources, clarify its chemical constituents, and find the relevant active compounds, the methanol extracts of non-medicinal parts of this plant were isolated using various chromatographic methods such as positive phase Sephadex LH-20, positive phase silica gel, positive phase polyamide, and MCI gel CHP-20P. The structures of the isolated monomer compounds were determined by MS, ¹H-NMR, 13C-NMR, and other spectral data analysis, as well as comparison with relevant literatures. The activities of the isolated compounds from S. kwangsiensis against bacteria(Escherichia coli, Staphylococcus aureus, Bacillus subtilis)and fungi(Fusarium oxysporum)were evaluated using filter paper method. The results were as follows:(1)A total of 10 compounds obtained from the non-medicinal parts of this plant were identified as vomifoliol(1), grasshopper ketone(2), isocorydine(3),2-O-β-D-glucopyranosyl-D-arabinitol(4),(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5), magnoflorine(6), corchoionoside C(7), tormentic acid(8), corydine(9)and crebanine(10). Among them, compounds 1, 2, 4, 5, 6, 7 and 8 were isolated from this plant for the first time, compounds 1, 2, 4, 5, 7 and 8 were firstly obtained from this genus plants, and Compound 4 was found from natural products for the first time.(2)At concentration of 6 mg·mL-1, compounds 1, 2 and 5 showed weak antibacterial activities against Escherichia coli; Compound 2 exhibited weak antibacterial activities against Staphylococcus aureus; compounds 3 and 8 displayed weak antibacterial activities against Bacillus subtilis. In conclusion, the non-medicinal parts of Stephania kwangsiensis contain large numbers of active components and have the potential of development and utilization. [ABSTRACT FROM AUTHOR]

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更年期女性卵巢功能逐渐衰退、雌激素水平下降,出现机体功能紊乱和代谢异常。其中,脂代 谢紊乱等可能诱发脑卒中、冠心病等疾病,严重影响更年期女性的身体健康。因此,该文对更年期女性脂代谢 紊乱的病因及其药物治疗研究进展进行综述,以期为改善更年期脂代谢紊乱,提高更年期女性的生活质量提供 参考依据。 [ABSTRACT FROM AUTHOR]

The experiment was to study the effect of larval powder of Hermetia illucens L. on production performance, egg quality, meat quality and serum biochemical indexes of Jianshui brown duck. A total of 120 Jianshui brown ducks were randomly divided into five groups, with two replicates in each group and 12 ducks in each replicate (ten laying ducks, two male ducks). Ducks in control group were fed with basic diet. Group 1, group 2, group 3 and group 4 used larval powder of Hermetia illucens L. to replace 2.5%, 5.0%, 7.5% and 10.0% soybean meal, respectively. The pre-test lasted for 10 d and the formal test lasted for 60 d. The results showed that compared with control group, the hatchability of ducks in each test group increased significantly (P<0.05), and the egg production of group 4 was the highest, and the fertilization rate of group 4 was significantly decreased (P<0.05). Compared with control group, the egg shape index of group 4 was significantly reduced (P<0.05), the eggshell thickness and eggshell strength of group 3 and group 4 were significantly reduced (P<0.05), and the Hastelloy units of group 2, group 3 and group 4 were significantly reduced (P<0.05). Compared with control group, the pH value of muscle in group 1 was significantly decreased (P<0.05), the dripping loss of group 1 and group 3 was significantly increased (P<0.05), the live weight, slaughter weight, half clean bore weight and full clean bore weight of each test group were significantly increased (P<0.05), the abdominal fat weight of group 2, group 3 and group 4 was significantly increased (P<0.05). Compared with control group, the activity of AST in duck serum of each test group was significantly increased (P<0.05). The study indicates that it is appropriate to replace soybean meal with Hermetia illucens L. at 5.0% to 7.5% in Jianshui brown duck diet. [ABSTRACT FROM AUTHOR]

Mixed land use is an important step to realize intensive land use and to stimulate regional vitality. This study examined the theory of mixed land use, constructed an analytical framework for the relationship between mixed use of rural residential land and rural vitality, and explored the characteristics of the impact of mixed use of rural residential land on rural vitality using Simpson ′ s diversity index, a multi-factor comprehensive evaluation method, and a correlation test of Shandong Province as an example. The results showed that the growth rates of rural vitality indexes in the high, medium, and low zones of mixed use of rural residential land in Shandong Province from 2010 to 2018 were 84.70%, 60.74%, and 50.33%, respectively. Thus, the faster the mixed use of rural residential land improved, the faster the development of rural vitality. The regional averages of the rural vitality index in the low, medium, and high zones of rural mixed use of residential land in Shandong Province were 0.13, 0.23, and 0.29 in 2010, respectively, and 0.19, 0.24, and 0.38 in 2018, respectively. The higher the degree of mixed use of rural settlement land, the higher the level of rural vitality. The Spearman correlation analysis and Kruskal–Wallis test results showed that the mixed use of rural residential land was related to rural vitality and stimulated rural vitality. This study concludes that mixed use of rural residential land promotes rural vitality and provides new ideas for innovative village planning and rural revitalization. [ABSTRACT FROM AUTHOR]

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To investigate the peripheral B lymphocyte phenotype in long-term survival allograft kidney transplant recipients. Peripheral B lymphocyte phenotype was detected in 54 Allograft kidney transplant recipients. The number (/μL) and percentage of total B cell, unswitched memory B cell, switched B cell, double negative B cell CD19+CD27-IgD- were recorded, respectively. The average estimated Glomerular Filtration Rate (GFR) was 91.63± 11.28 mL/min/1.73 m². The average time(years) after kidney transplant was 16.33 ± 5.98years. All patients had the same immunosuppressant treatment including cyclosporine. 1 Naïve B cells predominated in the peripheral B lymphocytes(37.92%± 22.06%), while unswitched B cells were the least(16.23%± 11.10%). The number of B lymphocytes subtypes correlated with the number of peripheral leukocytes and the percentage of neutrophils. 2 Correlation analysis indicated that, the percentage of switched B cells related to GFR (r=-0.279, P=0.045). Besides, the number of double negative B cells related to the trough level of cyclosporine (r=-0.300, P=0.029). Linear regression analysis indicated that the number of double negative B cells correlated with cyclosporine(R²=0.123, P=0.049). 3 Patients in the impaired allograft function team(GFR<90 mL/min/1.73 m², n=19) had higher percentage of switched memory B cells(23.61%± 10.96% vs.17.48%± 8.91%, P=0.030) than the normal allograft function team (GFR≥ 90 mL/min/1.73 m² n=35). Patients with lower cyclosporine trough level (<64 mmol/L, n=28) had more double negative B cells (13.74± 10.70 vs. 8.14± 6.72/μL, P=0.027) than the higher cyclosporine trough level patients(≥ 64 mmol/L, n=26). The percentage of switched memory B cells correlated with the normal/impaired allograft function team(r=-0.326, P=0.018). The number of double negative B cells correlated with the higher/lower cyclosporine team(r=-0.350, P=0.01). In the long-term survival kidney transplant patients with stable allograft function, naïve B cells predominated in the peripheral B lymphocytes. Switched memory B cells correlate with impaired allograft kidney function. Increased double negative B cells correlate with low cyclosporine trough level. [ABSTRACT FROM AUTHOR]

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Copyright of Telecommunications Science is the property of Beijing Xintong Media Co., Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Computer-Aided Design & Computer Graphics / Jisuanji Fuzhu Sheji Yu Tuxingxue Xuebao is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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This paper aimed to screen probiotics capable of inhibiting adhesion of enterohemorrhagic Escherichia coli(EHEC) and induction of IL-8 production by Caco-2 cells.[Methods] Strains were isolated from the intestines of healthy chickens and Lactobacillus with potential inhibition ability against E.coli were screened, and 16S rDNA sequencing was employed to identify the isolates. By formulating the environment of gastrointestinal fluid,the tolerance of Lactobacillus was evaluated. The inhibition of adhesion was detected by fluorescent labelling technique, and E.coli-induced IL-8 production was determined using an ELISA kit.[Results] The bacteriostatic circle diameter of ten Lactobacillus strains against tested E.coli isolates were all greater than 10 mm. 16S rDNA sequencing analysis got one L.johnsonii, one L. paraplanta and eight L. plantarum. Among them, Lactobacillus johnsonii NJ13 had the best tolerance to formulated gastric fluid with a gastric fluid survival rate of 73.19% for 3 hours, and could grow in the formulated intestinal fluid. L.johnsonii NJ13 was proved to inhibit the adhesion of E.coli ATCC35150 on Caco-2 cells and production of IL-8 induced by it. Besides, it can reduce the proportion of Enterobacteriaceae in infected mice to 0.000 205% and increase the proportion of Lactobacillus to 3.114 40%.[Conclusions] L.johnsonii NJ13 can inhibit the adhesion and damage of E.coli ATCC35150 to Caco-2 cells, placing a clue for the development and utilization of beneficial Lactobacillus resources and inhibitory way of EHEC infection. [ABSTRACT FROM AUTHOR]