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Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Hainan Medical University is the property of Journal of Hainan Medical College Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Chinese Teaching is the property of Beijing Language & Culture University Press Co. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Discuss the effect of catalpol on brain-derived neurotrophic factor (BDNF) expression in 茁-Amyloid peptide (A茁) intoxicated SH-SY5Y cells and the mechanism of regulating BDNF expression. Methods: The SH-SY5Y neuroblastoma cells induced by all-trans retinoic acid were used as the research object, and the effect of catalpol on A茁-injured cells was studied. The cell survival rate was determined by MTT assay, and the content of BDNF was determined by ELISA. The Western blot was used to detect the expression levels of transcription factors cAMP response element binding protein (CREB) and phosphorylated CREB (pCREB, activated form of CREB). The mRNA expression levels of BDNF and its series of transcripts were determined by RT-PCR. CREB expression was blocked by RNA interference and the expression of BDNF mRNA was determined by RT-PCR. Results Catalpol pre-protection increased the survival rate of SH-SY5Y cells after A茁 injury, significantly increased the content of BDNF in cell culture supernatant and intracellular BDNF mRNA level, promoted the expression of BDNF transcription factor IV and its key regulatory transcription factor pCREB. After interfered with CREB expression, the upregulation effect of catalpol on BDNF mRNA expression partially disappeared. Conclusion Catalpol may play a neuroprotective role by upregulating the phosphorylation of CREB and promoting the expression of BDNF. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Liquid Crystal & Displays is the property of Chinese Journal of Liquid Crystal & Displays and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To establish a prognostic prediction model for hepatocellular carcinoma (HCC) patients based on cuproptosis-related long non-coding RNA (LncRNA) and to validate its efficacy. Methods We obtained RNA expression data and clinical data of HCC from The Cancer Genome Atlas (TCGA) . Getting cuproptosis-related gene sets from literatures. Correlation analysis was used to screen cuproptosis-related LncRNAs. We used Lasso regression and Cox regression to screen LncRNAs with clinical prognostic significance and constructed a prognosis prediction model. The predictive efficacy of the model was evaluated by survival analysis, receiver operating characteristic (ROC) curve, and calibration curve. Results A total of 6 LncRNAs with independent prognostic significance (PICSAR, AC026356.1, MKLN1-AS, AL928921.1, LINC00479, and AC026412.3) were included to construct the prognostic prediction model. The survival analysis of the whole data set, test set and training set was carried out according to the high and low risk groups. The results showed that in three data sets, the overall survival time of the high risk group was shorter than that of the low risk group, and in the whole data set, the progression-free survival time of the high risk group was also shorter than that of the low risk group (both P<0. 05) . The number of sample deaths increased with the increase of risk score, and this conclusion was verified in the test set and the whole data set. The area under the curve of the risk model in predicting 1-, 3- and 5-year survival rates was 0.765, 0.664, and 0.649, respectively; compared with the other clinical factors [TNM stage (0.667), age (0.538), sex (0.512), and differentiation (0.507)], the area under the curve of the risk score model (0.765) was the largest. Univariate and multivariate Cox regression analysis showed that TNM stage and risk score were independent prognostic factors. According to the nomogram, its consistency index was 0.679 (95% CI：0.652-0. 705) . Combined with the calibration curve, the predicted survival rate was basically consistent with the observed survival rate. Conclusions A prognostic prediction model for HCC patients is successfully constructed based on 6 cuproptosis-related LncRNAs, and the model has high predictive efficacy after verification. [ABSTRACT FROM AUTHOR]

Objective: M1 muscarinic receptors are functional impairment in Alzheimer's disease (AD) and fail to respond to ligands properly. This study simulated the pathological state of Aβ oligomers enrichment in early AD, and investigated the effects on the activities of key molecules in the downstream signaling pathway of M1 muscarinic receptors, in order to provide new insights into the mechanism of M1 muscarinic receptors coupling disorder in AD. Methods: Aβ oligomers were successfully prepared in vitro. CHO-M1cells were pretreated with 1 μM Aβ oligomers for 30 min, 24 h, and 48 h, and then ACh or CCh agonists were adopted to activate the M1 muscarinic receptors. Calcium mobilization assay, IP-One-Gq KIT and Western blot were utilized to detect the downstream key signaling molecules Ca2+, IP1 and the dynamic changes of ERK phosphorylation after receptors activation. Results: Aβ oligomers preconditioning resulted in reduced Ca2+ and IP1 release and earlier activation of ERK in the downstream of the activated M1 muscarinic receptors. Conclusion: Aβ oligomers pretreatment lead to abnormal release and phosphorylation levels of key signaling molecules downstream of activated M1 muscarinic receptors, revealing that M1 muscarinic receptors are dysfunctional in the early stages of AD and cannot correctly activate signaling transduction pathways. [ABSTRACT FROM AUTHOR]

Copyright of China Textile Leader is the property of China Textile Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Northeastern University (Natural Science) is the property of Dongbei Daxue Xuebao and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Cotton Textile Technology is the property of Cotton Textile Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shanghai Jiaotong University (Medical Science) is the property of Journal of Shanghai Jiaotong University (Medical Science) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Research & Exploration in Laboratory is the property of Research & Exploration in Laboratory and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To study the protective effects of α-lipoic acid on oxidative stress and apoptosis of mouse ovary granulose cells cultured in vitro. Methods Granulosa cells were collected from PMSG-treated mice and cultured in DMEM/F12 medium for 24h; then the cell medium was supplemented with 100μmol/L α-lipoic acid for 24h culture. After α-lipoic acid treatment, SOD, MDA and GSH-Px activities in cell supernatant were measured. Additionally, RT-PCR, immunocytochemistry and Western blot were performed to detect the expressions of caspase-3 and caspase-9. Immunofluorescence and ELISA were used to evaluate the expressions of caspase-3 and caspase-9 at the protein level in whole granulosa cell lysate protein in both groups. Results α-lipoic acid supplementation increased SOD and GSH-Px activities and decreased MDA content notably (P<0.05). PCR products of caspase-3 and caspase-9 in granulosa cells were analyzed using RT-PCR and Real-time PCR. The expected 180 bp product was verified for caspase-3 and 152bp for caspase-9 by electrophoresis and gel imager in 'α-lipoic' acid group and control group. Expressions of caspase-3 and caspase-9 at mRNA level showed a decreased tendency in α-lipoic acid group (P<0.05). For immunofluorescence analysis, caspase-3 and caspase-9 protein of granulosa cells was detected. Cell-specific positive staining was located in the cytoplasm of the granulosa cells which showed green fluorescence for caspase-3 and caspase-9 protein; cell nuclei showed blue fluorescence. In α-lipoic acid group it has a lower bands density values than the control group by Western blot analysis (P<0.05). ELISA assay showed a significant decrease of caspase-3 and caspase-9 activities in α-lipoic acid group (P<0.05). Conclusion α-lipoic acid can significantly change the anti-oxidation ability of mouse ovarian granulosa cells, which suggests that it has protective and antioxidant effects on oxidative stress and apoptosis during the culture of mouse ovarian granulosa cells. [ABSTRACT FROM AUTHOR]