Objective To study the application of agglutinin chip to detect the changes in cell surface glycoprotein levels of suspended red blood cells stored in MAP during storage. Methods Partial samples were taken at 1,7,14,21,28, and 35 days after the red blood cells were stored in suspension, and red blood cell membrane proteins were extracted to prepare lectin chips. The fluorescence intensity changes of ACA, PNA, DSA, ECA, EEL, GSL-I-B4, HHL and NPL after binding to corresponding glycoproteins on the surface of red blood cells were detected. Results When suspended red blood cells were stored for 7 days, the fluorescence intensity of PNA and NPL combined with corresponding glycoproteins on the surface of red blood cells was lower than that of 1 day. When suspended red blood cells were stored for 14 days, the fluorescence intensity of ACA, DSA, ECA, GSL-I-B4 and HHL combined with corresponding glycoproteins on the surface of red blood cells was lower than that of 1 day. When suspended red blood cells were stored for 21 days, the fluorescence intensity of EEL combined with corresponding glycoproteins on the surface of red blood cells was lower than that of 1 day. Conclusion During the storage of suspended red blood cells stored with MAP, the glyco-protein level on the surface of the red blood cells will gradually decrease, and most of them will decrease significantly after 21 days of storage. During the preservation process, the level of certain immune-related glycoproteins on the surface of red blood cells is significantly reduced, which may affect its immune function. [ABSTRACT FROM AUTHOR]