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The study was conducted to investigate the effect of dietary supplementation of different levels of vitamin D3 on contents of vitamin D3, calcium, phosphorus and egg quality in Luhua eggs. A total of 252 healthy hens aged 40 weeks were randomly divided into seven groups with 36 chickens in each group. Hens in control group were 也d a basal diet （vitamin D3 level 3 600 IU/kg）, and hens in experimental groups were fed with diets supplemented with different levels of vitamin D3 （vitamin D3 level 6 600, 9 600, 15 600, 27 600, 51 600, 99 600 IU/kg）. On the 10th, 20th and 30th day, eggs were randomly selected from each group to determine the content of vitamin D3, calcium, phosphorus in eggs and egg quality. The results showed that the vitamin D3 content in yolk was increased with increasing of dietary vitamin D3 level. The content of vitamin D3 in egg yolk of 99 600 IU/kg vitamin D3 group reached the highest on the 30th day, which was four times that of the control group. The phosphorus content in egg white of 27 600, 51 600 and 99 600 IU/kg vitamin D3 groups was significantly higher than that of control group （P<0.05）. During the experiment, the yolk color of 99 600 IU/kg vitamin D3 group was significantly higher than that of control group （P<0.05）. The experiment indicates that increasing dietary vitamin D3 content can significantly increase its enrichment in egg yolk, increase phosphorus content in egg white in high concentration group significantly, and improve egg yolk color. [ABSTRACT FROM AUTHOR]

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