[Objective] 95 germplasm resources from Qinghai province were identified for resistance to stripe rust at seedling stage and adult stage, and their resistance genes were screened by molecular detection, which provided a basis for resistant breeding and rational distribu tion. [ Methods ]95 wheat varieties in Qinghai province were inoculated with CYR32, CYR33 and CYR34 for resistance identification at seed ling and adult stage, meanwhile, the closely linked markers of 7 resistance genes of Yr5, Yr9, Yr JO, Yrl5, Yr 17, Yrl8 and Yr26 were ana lyzed. [ Results] Identification of disease resistance at seedling stage: The results showed that 10 ( 11 % ) of CYR32 was for immune materials, and 85 ( 89%) was for high resistance ;44 ( 46% ) of CYR33 was for immune materials, 50 ( 53 % ) was for high resistance, and 1 ( 1 % ) was for medium resistance; 3 ( 3 % ) of CYR34 was for immune materials, 11 (12 % ) was for disease resistance, and 81 ( 85 % ) was susceptible materials. 12 varieties (lines) were resistant to CYR32, CYR33 and CYR34 simultaneously. Identification of disease resistance in adult stage: The comparison of resistance in two years showed that 12 wheat varieties ( 13%) were more resistant ;8 ( 8% ) were still re sistant, but the resistance was gradually weakened; 74 (78 % ) were less resistantand gradually evolved from resistant to susceptible. Molecu lar detection: Yr5 (78%), Y,9 (17%), YrlO (53%), Yr15 (76%), Yrll (78%), Yr18 (73%) and Yr26 (81%) were the materials which might carry the Yr series genes in the tested wheat varieties. [ Conclusion] Molecular detection results showed that genes Yr5, Yrl 5 and Yr26 were used frequently in wheat breeding at present, especially Yr26 was over-dependent. However, with the emergence of CYR34, many varieties had reduced or lost resistance to stripe rust. [ABSTRACT FROM AUTHOR]