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Copyright of Chinese Journal of Inorganic Analytical Chemistry / Zhongguo Wuji Fenxi Huaxue is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Inorganic Analytical Chemistry / Zhongguo Wuji Fenxi Huaxue is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The experiment was to investigate the mechanism of improvement and protective effect of sodium acetate on MAC-T cell steatosis by adding different concentrations of sodium acetate to bovine MAC-T cell steatosis model. MAC-T cells were stimulated by LPS to establish a steatosis model of MAC-T cells. Control group, LPS treatment group (cells were stimulated by 1 000 μg/L LPS for 9 h) and sodium acetate + LPS treatment group with different concentrations (2, 4 and 8 mmol/L sodium acetate were added to LPS treatment group, respectively) were established. The content of TG and the expression levels of ACC, FAS, SCD-1, CPT-1, CPT-2 and ACO of key genes of lipid anabolic metabolism were determined, and the content of TNF-α, IL-6 and IL-8 were detected. The results showed that after LPS stimulation for 9 h, the TG content of MAC-T cells was extremely decreased (P<0.01), and the total lipid drop area was significantly decreased (P<0.05). Compared with control group, the TG content of cells in LPS treatment group was significantly decreased (P<0.05), and the mRNA expression levels of ACC, FAS and SCD-1 genes related to lipid anabolic metabolism were extremely decreased (P<0.01). The mRNA expression levels of CPT-1, CPT-2 and ACO related to lipid catabolism were significantly up-regulated (P<0.05), the content of inflammatory factors TNF-α, IL-6 and IL-8 were significantly increased (P<0.05). Compared with LPS treatment group, the mRNA expression levels of ACC, FAS and SCD-1 were significantly up-regulated after adding 4 mmol/L sodium acetate (P<0.05), CPT-1 was significantly down-regulated (P<0.05), and the content of TNF-α and IL-6 were significantly decreased (P<0.05). TG content was significantly increased (P<0.05). After adding 8 mmol/L sodium acetate, the mRNA expression levels of ACC and FAS were significantly up-regulated (P<0.05), the mRNA expression levels of CPT-1 and CPT-2 were significantly down-regulated (P<0.05), and the content of TNF-α, IL-6 and IL-8 were significantly decreased (P<0.05). TG content was significantly increased (P<0.05). The study indicates that 4, 8 mmol/L sodium acetate can promote the lipid anabolic metabolism of MAC-T cells, promote TG synthesis, and have a certain protective effect on the inflammatory damage of MAC-T cells. [ABSTRACT FROM AUTHOR]