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Objective To explore the role of miR -103a-3p/regulator of calcineurinl (RCAN1) pathway in maltol aluminum induced memory impairment in rats. Methods In total 32 male SD rats were randomly divided into control group (0 (xmol/kg), low dose aluminum exposure group (10 (xmol/kg), medium dose aluminum exposure group (20 |Jimol/kg), and high dose aluminum exposure group (40 (xmol/kg), with 8 rats in each group. The rats in the control group were intraperitoneally injected with 0.9% normal saline, and the aluminum group was injected with maltol aluminum solution of corresponding concentration every other day for 3 months. After exposure, the learning and memory ability of rats was assessed by water maze test, the gene expression of miR-103a-3p and RCAN1 was detected by real-time fluorescence quantitative PCR, and the relative expressions of RCAN1, glycogen synthesis kinase-3 0 (GSK-3 |3), and phosphorylated tau protein in hippocampus were detected by Western blotting. Results The escape latency in the aluminum exposed group was higher than that in the control group in the first five days, and the residence time in the target quadrant and the number of times crossing the platform in the aluminum exposed group were lower than those in the control group (P<0.05) on the sixth day of spatial exploration. Compared with the control group, the expression level of miR103a-3p gene in hippocampus of aluminum exposed group decreased (P< 0.001), while the expression level of RCAN1 gene increased (P<0.001). Compared with the control group, the expression of RCAN1, GSK3 [3, and p-tau (ser396) protein in hippocampus of aluminum exposed group increased (P<0.001). Conclusion MiR-103a-3p/RCANl pathway is involved in the regulation of learning and memory impairment induced by aluminum. [ABSTRACT FROM AUTHOR]

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Bioprocess Engineering is the property of Chinese Journal of Bioprocess Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Materials Engineering / Cailiao Gongcheng is the property of Journal of Materials Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Over 90% of patients with osteoarthritis suffer from synovial lesions, and the occurrence of synovitis may also promote cartilage degeneration. To explore the potential mechanisms of synovial lesions is beneficial to find precise treatment targets and achieve good long-term prognosis. OBJECTIVE: To identify candidate hub genes in the synovial tissues during the development of osteoarthritis by bioinformatics analysis, and to further provide new insights for osteoarthritis study. METHODS: The osteoarthritis synovial-associated chip data sets GSE82107, GSE12021, GSE55457, and GSE55235 were downloaded from the public database GEO, including 40 cases of osteoarthritic synovial tissue and 36 cases of normal synovial tissue. R software was used to screen differentially expressed genes using Gene ontology function enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, and hub genes are selected by STRING online analysis tool and Cytoscape software. RESULTS AND CONCLUSION: Total 447 differentially expressed genes were selected between osteoarthritis and healthy control, including 201 up-regulated and 246 down-regulated genes. These differentially expressed genes were mainly enriched in inflammatory response, regulations of extracellular matrix, collagen, blood vessel development, as well as MAPK signaling pathway, tumor necrosis factor signaling pathway, arachidonic acid metabolism. Eight hub genes were screened by analyzing the protein interactions, which included matrix metalloproteinase 9, interleukin 6, vascular endothelial growth factor A, JUN, prostaglandin peroxide enzyme 2, CXCL8, MYC, epidermal growth factor receptor. The hub genes selected by bioinformatics analysis such as vascular endothelial growth factor A, matrix metalloproteinase 9, JUN, and prostaglandin peroxidase 2 may be biomarkers for the diagnosis of osteoarthritis and potential targets for treatment. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)