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Objective: To explore the expression level of NDRG2 protein in the lens tissues of the nuclear age related cataract patients and the possible mechanism. Methods: Collect lens without lens capsules from patients with nuclear age-related cataract undergoing surgical in First Affiliated Hospital, Harbin Medical University. Lens was grading though Emery method. And preliminarily adjust the concentration of each sample based on the protein concentration results. Detection of NDRG2 and Laminin γ2 expression level in different nuclear grading lenses by Western blot. HLE B-3 lens epithelial cell line was induced by 1200 μM H2O2 for 96h to establish the aging-realated cataract model. HLE B-3 cells without treated with H2O2 were set as the control group. Collect lysates from the experimental group and the control group, and detect NDRG2 and Lamininγ 2 in the two groups by Western blot. Further pCDNA3.1-NDRG2/ Lamin γ2 plasmids were transfected into HLE B-3 cells to explore the possible mechanism of NDRG2 involvement in different nuclear age related cataracts. Results: NDRG2 and Laminin γ2 were much higher in nuclear age-related cataract. The higher the nuclear grade, the more protein expression. In the H2O2 treated HLE B-3 cell aging model, the expression level of NDRG2 and Laminin γ2 were much higher than the control group. NDRG2 overexpression couled up-regulate expression of Lamin γ2, but Laminin γ2 overexpression had little influence to expression level of NDRG2. Conclusion: The expression of NDRG2 in nuclear age-related cataract lens tissues were increased and positively correlated with the nuclear grade, which might take effect by the upregulation of Laminin γ2. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To observe and compare the clinical efficacy of phacoemulsification and intraocular lens implantation combined with goniosynechialysis or trabeculectomy in the treatment of acute primary angle-closured glaucoma with cataract. Methods:62 cases of patients diagnosed with acute angle-closure glaucoma combined with cataract in the Eye Hospital of the First Affiliated Hospital of Harbin Medical University from January 2017 to November 2017 were selected and randomly divided into group A and B according to different operation methods, group A(31 cases, Phaco+IOL combined goniosynechialysis); group B(31 cases, Phaco + IOL combined trabeculectomy). The best corrected visual acuity(BCVA), intraocular pressure(IOP), anterior chamber depth(ACD), the changes of corneal endothelial cell density(ECD) after operation were compared between two groups of patients. Results: The BCVA and IOP of both groups of patients at 3 days and 3 months after operation were significantly improved(P<0.05), the IOP returned to the normal range. Furthermore, at 1 week after operation, the visual acuity of group A was better than that of group B(P<0.05). At three months after operation, the ACD and ECD of both groups changed significantly compared with those preoperation(P<0.05), and the ACD of group A was significantly deeper than that of group B(P<0.05). Conclusions: Phaco+IOL combined goniosynechialysis can effectively reduce intraocular pressure, obtain good clinical effect at the early satge in the treatment of acute primary angle-closured glaucoma with cataract. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effectiveness and security of anterior chamber intraocular lens implantation for high myopia in short-term. Methods: Thirty-six eyes of eighteen high myopia patients received ICL implantation were selected. The uncorrected visual acuity(UCVA), best corrected visual acuity(BCVA), spherical equivalent(SE), anterior chamber depth(ACD), intraocular pressure(IOP)and endothelial cell density(ECD) were detected preoperation and at 1 week, 1 month and 3 months postoperation. Results: After operation, the UCVA and SE were significantly improved(P<0.05), the mean ACD and ECD were significantly decreased(P<0.05). IOP showed no variation postoperation(P>0.05). Conclusions: ICL implantation appeared to be an effective and safe method for high myopic. [ABSTRACT FROM AUTHOR]

Copyright of Research of Environmental Sciences is the property of Research of Environmental Sciences Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Engineering Geology / Gongcheng Dizhi Xuebao is the property of Journal of Engineering Geology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the effect of truncated rhtBIGH3 protein in inhibiting the biological activities of HUVECS.Methods: Human umbilical vein endothelial cells（HUVECS） were cultured with VEGF in vitro, supplemented with 0 μg/mL and 100μg/mL rhtBIGH3-（RGD)2 protein. HUVECS were incubated for 24 h, 48 h, 72 h, and testing Caspase-3 activities and miR-126 expression level. Added miR-126 mimic and miR-126 inhibitor when the rhtBIGH3-（RGD)2 concentration was 0 μg/mL and 100 μg/mL,after 48 h, use Real-time PCR testing the miR-126 expression level, and test the apoptosis though the activity of Caspase-3. Results: In the VEGF-HUVEC supplemented with 100 μg/mL rhtBIGH3-（RGD)2 protein, the expression of both Caspase-3 and miR-126 raised, and reach the peak at 48 h. In VEGF-HUVEC, when the final concentration of rhtBIGH3-（RGD)2 protein was 100 μg/mL, after the addition of mimic miR-126, miR-126 and Caspase-3 expression increased, after adding inhibitor 48 h, miR-126 and Caspase-3 expression decreased. Conclusions: The truncated rhtBIGH3-（RGD)2 protein promote apoptosis and inhibit HUVEC activity by increasing miR-126 expression, thus inhibiting corneal neovascularization. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)