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Objective To investigate the effects of dexmedetomidine (Dex) on osteoporosis (OP) rats and possible mechanisms. Methods The rats were divided into sham operation group, osteoporosis model group (OP, replicating the OP rat model with bilateral ovariectomies), Dex-L, M, and H (Dex low, medium, and high dose treatments) groups and Dex-H+XAV-939 group (Wnt/β-catenin pathway inhibitor). Micro-CT was applied to measure bone mineral density (BMD) and bone microstructure of rat femurs. The three-point bending experiment was applied to analyze the biomechanics of the femur (maximum load, fracture deflection, elastic modulus). HE staining was applied to observe pathological changes in the femur of rats. ELISA method was applied to evaluate bone metabolism indicators such as alkaline phosphatase (ALP), typeⅠ procollagen amino-terminal peptide (PINP) and typeⅠcollagen cross-linked C-telopeptide (CTX-Ⅰ). The expression of Runx2 and Wnt3a was examined by Immunohistochemistry. Western blot was applied to detect the protein expression of Runx2 and Wnt3a/β-catenin pathway in femoral tissue. Results Compared to the Sham group, the bone volume and number of trabeculae in OP group were obviously reduced, the maximum load, fracture deflection, elastic modulus, BMD, Tb.Th, Tb.N, BV/TV, ALP, PINP, Runx2, Wnt3a, β-catenin expression decreased, CTX-Ⅰ increased (P＜0.05). Compared to the OP group, the bone trabecular structure in the Dex-L, M, and H groups was restored, the maximum load, fracture deflection, elastic modulus, BMD, Tb.Th, Tb.N, BV/TV, ALP, PINP, Runx2, Wnt3a, β-catenin expression all increased but CTX-Ⅰ decreased (P＜0.05). Compared to the Dex-H group, the bone trabecular injury in the Dex-H+XAV-939 group showed a more severe damage. The maximum load, fracture deflection, elastic modulus, BMD, Tb.Th, Tb.N, BV/TV, ALP, PINP, Runx2, Wnt3a, β-catenin expression decreased while CTX-Ⅰ increased (P＜0.05). Conclusions Dex may antagonize OP effects by improving bone density, biomechanical properties and microstructure. The underlying mechanism might be related to the activation of the Wnt/β-catenin signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Archives Science Bulletin is the property of Editorial Office of Archives Science Bulletin, Renmin University of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Harbin University of Science & Technology is the property of Journal of Harbin University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

白内障是临床常见的致盲性眼病，白内障超声乳化吸除联合人工晶状体植入术是其最主要的治疗手段。 以往临床植入的晶状体多为单焦点人工晶状体，这种晶状体对患者术后远距离视力恢复效果较好，而对中近距离视 力恢复效果不明显。景深延长型（EDOF）人工晶状体通过延长单一焦点而加强景深，不仅能够提供良好的远、中、 近距离视力，而且术后对比敏感度下降较少，也不增加术后眩光、光晕现象。但 EDOF人工晶状体近距离视力要略 逊于多焦点人工晶状体。未来研究的重点应是寻找最佳全程裸眼视力、清晰成像质量和最小眼内散射光现象之间 的平衡，从而为白内障患者提供最佳的全程视力。 关键词：白内障；景深延长型人工晶状体；临床应用 [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To probe into the effects of dezocine on the proliferation and differentiation of osteoblasts MC3T3-E1 and the Wnt/ β-catenin signaling pathway. METHODS: MC3T3-E1 cells were treated with dezocine at concentrations of 0, 5, 10, 20, 40 and 80 μg/ mL for 48 hours, cell counting kit-8 (CCK-8) was used to detect the cell survival rate and calculate the half inhibitory concentration (IC50 ). MC3T3-E1 cells were divided into control group, dezocine low concentration group (10 μg/ mL), dezocine medium concentration group (20 μg/ mL), dezocine high concentration group (40 μg/ mL) and dezocine high concentration+ lithium chloride (LiCl) group (40 μg/ mL+ 20 μmol/ L), the CCK-8 was used to detect cell survival rate; alkaline phosphatase (ALP) detection kit was used to detect the ALP activity; the alizarin red staining was used to detect cellular calcified nodules; the Western blot was used to detect the expression levels of osteogenic differentiation related protein [Runt-related transcription factor 2 (RUNX2), osteopontin ( OPN), osteocalcin ( OCN)] and Wnt/ β-catenin pathway related proteins ( Wnt3a, β-catenin) in the cells. RESULTS: The IC50 of MC3T3-E1 cells to dezocine was about 20 μg/ mL; compared with the control group, the cell survival rate, ALP activity, number of calcified nodules, expression levels of RUNX2, OPN, OCN, Wnt3a and β-catenin protein in dezocine low, medium and high concentration groups were decreased successively, with statistically significant difference (P<0. 05); compared with dizocine high concentration group, the cell survival rate, ALP activity, number of calcified nodules, the expression levels of RUNX2, OPN, OCN, Wnt3a and β-catenin protein in dezocine high concentration+LiCl group were significantly higher, with statistically significant difference ( P < 0. 05). CONCLUSIONS: Dezocine can inhibit the proliferation and differentiation of osteoblasts MC3T3-E1 by inhibiting the Wnt/ β-catenin signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Petroleum Machinery is the property of China Petroleum Machinery Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Petroleum Machinery is the property of China Petroleum Machinery Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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