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Objective: To analyze the characteristics of intrauterine microbiota in infertile women with poor endometrial receptivity (PER) and identify its biomarkers. Methods: A total of 63 infertile women who visited our gynecology department from July 2021 to May 2022 were selected. According to ultrasound evaluation of endometrial receptivity, 40 cases were assigned to the PER group, while 23 cases with good endometrial receptivity were assigned to the control group. Endometrial tissue samples were collected from two groups of patients for 16S rRNA sequencing, to compare the characteristics and differences of intrauterine microbiota between the two groups. Results: There was no significant difference in the α diversity of intrauterine microbiota between the two groups(P＞0.05). However, the individual difference of intrauterine microbiota in PER group was significantly higher than that in control group (P＜0.05). The relative abundance of the dominant phylum Proteobacteria in the intrauterine of the PER group was significantly higher than that in the control group (50.52% vs. 19.35%). The dominant genus in both groups was Lactobacillus, but the relative abundance of Lactobacillus in the intrauterine of the PER group was significantly lower than that of the control group (21.76% vs. 45.96%), while the relative abundance of Ralstonia was significantly higher than that in the control group (18.01% vs. 0.48%). The relative abundance of Ralstonia_unclassified in the intrauterine microbiota of the PER group was significantly higher than that in the control group (18.01% vs. 0.48%), while the relative abundance of Lactobacillus_crispatus was significantly lower than that in the control group (3.82% vs. 23.78%). The Biomakers at the phylum, family, and genus levels of the intrauterine microbiota in the PER group are Proteobacteria; Burkholderiaceae, Vibrionaceae, etc; Ralstonia, Vibrio, etc. Conclusions: Infertility women with PER may have dysbiosis of the intrauterine microbiota, with a decrease in the relative abundance of Lactobacillus and an increase in the relative abundance of characteristic pathogenic bacteria such as Burkholderia and Ralstonia possibly related to PER. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of cervicitisstatus on human papilloma virus(HPV) infection. Methods: The E6-c-Myc-P2A-E7-c-3Flag gene lentivirus was used to infect the control group (untreated human normal cervical epithelial cells) and the experimental group [human normal cervical epithelial cells induced with lipopolysaccharide (1 μg/mL) for 24 h], respectively, and observe the effect of cervicitis status on the expression of Myc-E6 and Flag-E7 tagged proteins. Results: Compared with the control group, the expression of human interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), IL -1β, and IL -17A mRNA was significantly increased in the experimental group, and the differences were statistically significant (both P＜0.05). After 72 h of infection with the E6-c-Myc-P2A-E7-c-3Flag gene lentivirus, the transfection efficiency of the experimental group and the control group was (89.05 ±0.16)% and (79.30 ±1.97)%, respectively, with a statistically significant difference (P＜0.05). The expression of Myc-E6 and Flag-E7 tagged proteins in the experimental group was significantly increased compared to the control group, and the difference was statistically significant (both P＜0.05). Conclusions: Cervical epithelial cells are more susceptible to HPV infection when they are in an inflammatory state. [ABSTRACT FROM AUTHOR]

Polycystic ovary syndrome (PCOS) is one of the most common reproductive endocrine disease in women of reproductive age. It is affected by traditional Chinese culture, fertility concepts, social, economic, family and the disease, PCOS infertility patients often have impaired psychological regulation function, showing more obvious negative emotional characteristics such as depression and anxiety. Negative emotion and disease are causal to each other, they influence each other and form a vicious circle, affecting or aggravating the clinical features, sex hormone level and disorder of glucose and lipid metabolism of PCOS patients, which in turn leads to the occurrence of infertility, and the adverse effects of pregnancy outcomes. The positive psychological intervention and treatment can improve the patients′ psychological state, eliminate or alleviate the negative emotions such as anxiety, thus improve the treatment outcome of PCOS infertility. This article summarizing from the above aspects, is expected to provide new ideas for clinical treatment of patients with PCOS infertility. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To analyze the distribution characteristics of vaginal microecology and cervical human papilloma virus (HPV) in patients with vulvovaginal pruritus, dryness, pain and/or abnormal secretion, and to explore the relationship between vaginal microecology disorders and HPV infection. Methods: From February 1, 2019 to December 31, 2019, 483 patients with vulvovaginal pruritus, dryness, pain and/or abnormal secretion in the gynecological clinic of the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine were selected as the study subjects. Simultaneously, vaginal microecology and cervical HPV were detected and the results were analyzed. Results: Among the 483 patients, only 4 cases had normal vaginal microecology (0.8%) and 479 cases had microecology disorders (99.2%). of which 350 cases (73.1%) had microecological disorders (i.e. vaginal infections) with clear pathogens and 129 cases (26.9%) without clear pathogens. Among 350 cases of vaginal infection, 287 cases (82.0%) were single infection and 63 cases (18.0%) were mixed infection. The single infection of VVC (38.7%, 111/287) and mixed infection (79.4%, 50/63) with VVC had the highest incidence. The proportion of patients with decreased Lactobacillus and decreased H2O2 in vaginal infection was higher than that in vaginal microecological disorder without clear pathogens, and the difference was statistically significant (P＜0.05). Of the 483 patients, 162 were infected with HPV, at a rate of 33.5%. The HPV infection rate in vaginal infection (37.1%, 130/350) was significantly higher than that in vaginal microecological disorders without clear pathogens (24.0 %, 31/129). Among them, the HPV infection rate of patients with trichomonal vaginitis (TV), bacterial vaginosis (BV), aerobic vaginitis (AV), VVC, and BV intermediate were 73.3% (11/15), 52.9% (72/136), 34.5% (10/29), 29.2% (47/161) and 27.4% (20/73), respectively. Multivariate regression analysis showed that TV and BV were positively correlated with HPV infection (OR were 5.325 and 3.298, respectively, P＜0.05 ). Conclusions: Vaginal microecological disorders are common in patients with vulvovaginal pruritus, dryness, pain, and/or abnormal secretion, and they are susceptible to HPV infection. When vaginal infection occurs (especially TVand BV), the risk of HPV infection is greatly increased. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Embryonic development begins from the zygote. After multiple cleavages, the embryonic morphology changed, and the internal cells differentiated into the different lineages that laid the foundation for the subsequent formation of gastrula. The various signal pathways participate in the fine regulation of early embryonic development. For example, the differential activation of Hippo signal pathway leads to the differentiation of trophectoderm (TE) and inner cell mass (ICM); fibroblast growth factor/extracellular signal-regulated kinase (FGF/ ERK) signaling pathway further differentiated ICM into primitive endoderm (PE) and epiblast (EPI); bone morphogenetic protein (BMP) signaling pathway and classic Wnt signaling pathway are not only related to the pluripotent of embryonic cells, but also play an important role in the establishment of hypocotyls; Notch signaling pathway and Nodal signaling pathway are mainly involved in the formation of left-right asymmetry of embryos. Here we review the role and regulation mechanism of the main signal pathways in early embryonic development, so as to provide reference for the in -depth understanding of the process of early embryonic development and regulation. [ABSTRACT FROM AUTHOR]

Patients with ovarian cyst treated by gynecologic surgery in the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine from May 2013 to May 2017 were selected. Patients with ovarian endometriosis cysts confirmed by pathology after surgery were the observation group, patients with simple cysts were the control group. The parameters of two groups were compared: platelet count (PLT), plateletcrit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), large platelet count (P-LCR), prothrombin time (PT), thrombin time (TT), activated partial thrombin time (APTT), fibrinogen (FIB), prothrombin international ratio (INR), plasma D-dimer and serum CA125. To evaluate the diagnostic value of platelet and coagulation parameters in ovarian endometriosis cysts. Results: Compared with the control group, PLT, PCT, CA125 were higher and PDW, APTT were lower in the observation group, the difference was statistically significant (P＜0.05). Compared with the observation groupⅠ, Ⅱ, PLT, PCT and MPV were higher and PDW、P-LCR、APTT were lower in the observation group Ⅲ, Ⅳ, the difference was statistically significant (P＜0.05). There was a positively correlated between CA125 and MPV，negative correlation between CA125 and PDW, P-LCR in the observation group. Compared with preoperative, postoperative PLT, PCT and MPV were lower, PDW, P-LCR were higher in observation group, the difference was statistically significant (P＜0.05). [ABSTRACT FROM AUTHOR]

The cesarean scar defects（CSD） is the hypoechoic area of the lower segment of the uterus under ultrasound. It is the absence or rupture of the myometrium at the incision of the previous cesarean section, which affects about 30% of women. At present, the cause of CSD is not clear, but studies have found that low incision position, single suture, using chrome gut, psychological pressure, posterior position of uterus, multiple cesarean section are risk factors of CSD. Double layer suture, peritonealsuture and absorbable suture are good for incision healing. In addition, the formation of CSD is related to the longterm use of corticosteroids, history of adenomyosis, history of vaginal delivery, history of pre-eclampsia. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective：To investigate the demand of treatment and health care of menopausal syndrome in middle-aged and old women, and the menopause-related knowledge, so as to provide the scientific guidance for the clinical health care of menopause. Methods：A stratified random cluster sampling was used to select women aged 40 to 60 years in Tianjin, to conduct a questionnaire survey. Results：A total of 2 400 eligible women were investigated in this study, and 2 018 eligible questionnaires were collected (the eligible rate, 84.08%). Of the 2 018 women interviewed, 1 212 were naturally menopausal, with an average age of (49.73±3.53) years. A survey of 1 623 cases of menopausal transition, early and late menopause showed that 9.61% (156/1 623) of women were receiving the treatment of menopausal syndrome, 69.23% (108/156) of whom received traditional Chinese medicine, and only 9.62% (15/156) received hormone therapy. 42.27% (686/1 623) women thought that menopausal syndrome needed treatment, 72.01% (494/686) of them tended to be treated with traditional Chinese medicine, but not with hormone alone. 65.06% (1 056/1 623) women received the education on menopause-related health knowledge, 61.27% (647/1 056) women got health knowledge from the media, 15.53% (164/1 056) women from medical institutions. Totally, 66.67% (1 082/1 623) women wanted to know about the health care-releted knowledge. Conclusions： Middle -aged and old women are eager to know about menopause. However, the professional guidance from medical institutions is insufficient. Compared with hormone replacement therapy, traditional Chinese medicine therapy has a higher acceptance in Chinese women. [ABSTRACT FROM AUTHOR]

Endometriosis is a common and frequently encountered disease of gynaecology, it is a kind of chronic benign disease, affecting 10% to 15% of the women of childbearing age, with main symptoms of dysmenorrhea, pelvic adhesions and infertility, but its pathogenesis has not yet known clearly, many of the hypothesis. At home and abroad in recent years, scholars have found that platelets play an important role in the incidence of endometriosis, the main performance of platelet adhesion, aggregation function in patients with endometriosis was flawed, platelet count in patients with endometriosis, its relatively high blood coagulation state, and can be mediated inflammatory response and platelet associated factors such as P-select element, CD40L, platelet growth factors in the pathogenesis of endometriosis have different degrees of promoting function, platelet can affect patients with endometriosis and the expression of estrogen and its receptors, so the review on platelet abnormal function with the pathogenesis of endometriosis may exist, for the clinical application of platelet and provide theoretical basis for treatment of endometriosis. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To get the endometrium stem cells （ＥＳＣ） for the eutopic endometrial from women with endometriosis （ＥＭｓ） by using colony-forming assay and provide the experimental basis for the further study of EMs. Methods: The endometrial samples from women with EMs who treated in Department of Obstetrics and Gynecology, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine in 2012 -2014 undergoing fractional curettage respectively. Colony-forming cells （ＣＦＣｓ） were isolated from individual colony-forming units （ＣＦＵ） which visible macroscopically and contained ＞4 000 cells. We focused on the characteristics of stem cells between CFCs and N -CFCs, including high proliferative potential （ＭＴＴ ａｓｓａｙ）, serial clone formation, mammosphere formation of self-renewal, differentiation potential in vitro, and tissue reconstitution in vivo. Results: The optical densities in cell proliferation were more significantly higher in the CFCs than N-CFCs （Ｐ＜０．０５）. The cloning efficiency （CE） of CFCs from P1-5 are higher than N-CFCs [CFCs: （１２．３０±３．７５）%, （１３．１１±３．２３）%, （11.86±2.21）%, （１２．０８±３．０１）%, （１１．０１±３．９８）%; N-CFCs: （５．５７±２．１３）%, （４．２１±２．１２）%, （２．２３±１．０２）%, （０．１２±０．０７）%, 0%]. There are statistical significances between the two groups （Ｐ＜０．０５）. CFCs originated spheres with a diameter were at least 60 m, spheres were continuously generated at 5-6 day intervals for at least 3 passages with a bigger and bigger size，in contrast, the spheres of N-CFCs cannot be passaged serial [Count the cells of each passage: CFCs: （０．８２±０．３７）× 104 , （１．４５±０．６４）×104 , （３．０６±１．７３）×104 , N-CFCs: （０．４８±０．２７）×104, （０．３７±０．１５）×104, 0]. There are statistical significances between the two groups （P＜0.05）. The protein expression of CFCs was negative for CK-19 before induction culture, but were expressed at CFCs, which cultured with the induction medium for 21 days. The endometriotic lesion-forming efficiency （１００％） in the CFCs group was higher than that of N -CFCs group （４０．６３％）, when CFCs and N -CFCs were inoculated into the abdominal subcutaneous of nude mice, respectively. Reconstruction of endometriotic lesions in the CFCs group with an average volume at （30.95 ±12.13）mm3 was bigger than the N-CFCs group （１９．１２±１０．９８）mm3. Conclusions: There are stem cells in the function layer of endometrium in EMs patients and it can be isolated from eutopic endometrium using colony-forming assays. The study has given a simple method to get ESC easily for researching EMs. Endometrial stem cells can be cryopreserved and subcultured, and have the ability of unlimited proliferation, self-renewal, directional differentiation and ectopic lesion fomation. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To analyze the invasion and metastasis of endometrial stromal cells in endometriosis through assessing the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA. Methods Ectopic and eutopic endometrial tissues from patients with EMs (endometriosis) or uterine fibroids were collected and cultured. The fourth generation of purified endometrial stromal cells were used for research, and the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA were analyzed by RT-PCR. Results There were no statistical differences in transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA in eutopic endometrial stromal cells from patients with EMs or uterine fibroids between secration stage and proliferation stage（P＞0.05）. The transcription levels of COX-2, PGE2 and Snail mRNA were significantly in⁃ creased while transcription level of E-cadherin mRNA was decreased in eutopic and ectopic endometrial stromal cells in patients with EM compared with those in patients with uterine fibroid. And the difference was statistically significant（P＜0.05 or P＜0.01）. The transcription levels of COX-2, PGE2 and Snail mRNA were obviously higher while transcription level of E-cadherin mRNA was lower in ectopic endometrial stromal cells than those in eutopic endometrial stromal cells from patients with EMs. And the difference was statistically significant（P＜0.05 or P＜0.01）. Conclusion The invasion and metastasis of endometrial stromal cells of patients with EMs were remarkable than those cell of patients without EMs. What's more, the invasion and metastasis in ectopic endometrial stromal cells were more obvious than those cells in the pathogenesis of EMs. [ABSTRACT FROM AUTHOR]