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Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Chitosan material has good biocompatibility, biodegradability and low immunogenicity, but its activity is poor; mineralized collagen material has good biocompatibility, but its mechanical properties are insufficient, so their combination may show better mechanical properties and osteogenic activity. OBJECTIVE: To construct a chitosan/mineralized collagen porous scaffold with different compositions and to show surface characteristics and biocompatibility. METHODS: The porous scaffolds with different mass ratios (2:1, 1:1) of chitosan and mineralized collagen were prepared by freeze-drying. Scanning electron microscope, energy dispersive spectrometer, X-ray diffraction, and Fourier infrared Spectrum were used to show the surface morphology, element composition, and structure of porous scaffolds in each group. The mouse osteoblast precursor cells were directly inoculated on the surface of the scaffolds of three groups, and the cell adhesion was observed by scanning electron microscope. The blank control and three groups of material extracts were co-cultured with mouse osteogenic precursor cells. CCK-8 assay, phalloidin staining, and alkaline phosphatase analysis were applied to analyze the effect of materials on proliferation and differentiation of mouse osteogenic precursor cells. RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that there were uniform pores on the surface of chitosan composite materials. The interface of chitosan/mineralized collagen porous scaffold was tightly bonded. Simultaneously, as the content of chitosan in the scaffold decreased, the agglomeration of mineralized collagen particles in the scaffold became more obvious. Energy dispersive spectrometer displayed that elements on the surface of the simple chitosan scaffold were mainly carbon, nitrogen and oxygen. The calcium and phosphorous elements on the surface of the scaffold material gradually increased after mineralized collagen was added. X-ray diffraction and Fourier infrared Spectrum exhibited that chitosan and mineralized collagen were physically combined. (2) Scanning electron microscopy showed that the cells adhered and spread normally on the surface of the three groups of scaffold materials. Among them, the number of cells attached to the surface of the simple chitosan scaffold was less. (3) CCK-8 assay displayed that compared with the simple chitosan porous scaffold extract, two ratios of chitosan/mineralized collagen porous scaffold extract could promote the proliferation of mouse osteogenic precursor cells. Cytoskeleton staining showed that two ratios of chitosan/mineralized collagen porous scaffold had high cell density of the extract liquid group; the cell filament feet were connected to each other; and the expression of microfilament actin was clear. (4) The alkaline phosphatase activity test demonstrated that compared with the simple chitosan porous scaffold extract, the two ratios of chitosan/mineralized collagen porous scaffold extract can promote osteogenic differentiation of mouse osteogenic precursor cells. (5) It is concluded that chitosan/mineralized collagen porous scaffold can effectively promote proliferation and differentiation of mouse osteoblast precursor cells. [ABSTRACT FROM AUTHOR]

BACKGROUND: The structure of mineralized collagen/Mg-Ca composite prepared by freeze-drying method is loose, and the structural stability of the composite can be enhanced by using polymethyl methacrylate bone cement as the outer strengthening layer. OBJECTIVE: To investigate the effects of polymethyl methacrylate/mineralized collagen/Mg-Ca composite on osteogenic differentiation of MC3T3-E1 cells. METHODS: The polymethyl methacrylate/mineralized collagen/Mg-Ca and polymethyl methacrylate/mineralized collagen composites were prepared respectively. The MC3T3-E1 cells were directly inoculated on the surface of polymethyl methacrylate/mineralized collagen/Mg-Ca composite (group A), polymethyl methacrylate/mineralized collagen composite (group B) and Mg-Ca alloy (group C). Cells cultured alone were blank controls (group D). The cell adhesion morphology on the material surface was observed under the scanning electron microscope. CCK-8, alkaline phosphatase activity, enzyme linked immunosorbent assay, RT-PCR, and western blot assay were used to analyze the effects of three materials on MC3T3-E1 cell proliferation and osteogenic differentiation. RESULTS AND CONCLUSION: (1) Scanning electron microscope showed that the largest adhesion number of MC3T3-E1 cells on the surface of polymethyl methacrylate/mineralized collagen/Mg-Ca composite, grew well, stretched obviously, pseudopodia extended more, and connected with each other to form a network structure. (2) CCK-8 assay showed that compared with the group D, the three materials could promote the proliferation of MC3T3-E1 cells (P < 0.05), and the composite had the most significant effect in group A (P < 0.05). (3) Compared with the group D, the three materials could promote the alkaline phosphatase activity in MC3T3-E1 cells (P < 0.05), and the group A was the most significant (P < 0.05). (4) Enzyme linked immunosorbent assay showed that the three materials could promote the secretion of osteocalcin and type I collagen in MC3T3-E1 cells, and the promotion effect of group A was the most significant (P < 0.05). (5) RT-PCR showed that the three materials could increase the mRNA expression of RUNX2, OSX, alkaline phosphatase and osteocalcin, especially in group A (P < 0.05). (6) Western blot assay showed that the three materials could increase the protein expression of RUNX2, OSX, alkaline phosphatase and osteocalcin, and the group A had the most significant increase (P < 0.05). (7) The results showed that polymethyl methacrylate/mineralized collagen/Mg-Ca composite could promote the expression of osteogenic differentiation related proteins and genes in MC3T3-E1 cells, and had a positive effect on the osteogenic differentiation. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To analyze and evaluate the application value of dry fluorescent luminescence in the diagnosis and treatment of HBV infection. Methods A total of 397 serum samples were collected from patients attending Liver Diseases Department of the Third People's Hospital of Kunming from June 1, 2020 to December 31, 2020. Among these samples, 360 were detected for HBsAg, HBsAb, HBeAg, HBeAb and HBcAb by dry fluorescent luminescence method and ELISA, and 37 HBsAg positive samples in HBeAg seroconversion period detected by electrochemiluminescence method were tested for HBsAg, HBeAg and HBeAb by three methods: dry fluorescent luminescence method, enzyme-linked immunosorbent assay and colloidal gold method. The positive coincidence rate, negative coincidence rate and total coincidence rate of each test item by dry fluorescent luminescence method in diagnosis and treatment of HBV infection were compared and analyzed. Results The S/C value of ELISA was consistent with the trend of S/C value of dry fluorescent luminescence method, showing a certain positive correlation (r = 0.819, 0.913, 0.906, 0.732, 0.842, P < 0.001) . The positive coincidence rate, negative coincidence rate and total coincidence rate of the five items of HBV by the two detection techniques were more than 90%, with high consistency (Kappa = 0.949, 0.944, 0.955, 0.842, 0.949). The results of 37 serum samples from HBsAg positive HBeAg seroconversion period showed that the coincidence rate between dry fluorescent luminescence method and Roche electrochemiluminescence HBeAb was 100% (37/37), much higher than that of ELISA 18.92% (7/37) and colloidal gold 5.40% (2/37). Conclusion Dry fluorescent luminescence method can effectively reflect the serological conversion period of HBeAg. Conventional HBV screening test and enzyme-linked immunosorbent assay have a high consistency. At the same time, it has the advantages of short detection time, no frequent manual operation and simple operation, so it has a good application prospect. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Preliminary study has shown that the composite materials composed of magnesium-based materials and mineralized collagen have a good supporting effect on repairing the critical defects, which can improve the mechanical strength of mineralized collagen and premature collapse during bone healing to some extent. However, magnesium-based metals degrade fast in chloride-containing solutions (including human body fluids or plasma), and the effects of releasing magnesium ions on the proliferation and differentiation of osteoblasts are unknown. OBJECTIVE：To investigate the effects of magnesium ion combined with mineralized collagen on osteogenic differentiation of mouse preosteoblasts in vitro. METHODS: Mineralized collagen exacts were prepared from complete medium with magnesium ion concentration of 0, 5, 10, and 20 mmol/L. Mouse preosblasts were cultured with four mineralized collagen extracts, respectively, which were divded into mineralized collagen group, and 5, 10 and 20 mmol/L Mg2++ mineralized collagen groups. The mouse preosblasts cultured in complete medium were used as control group. The cell morphology, proliferation, apoptosis，intracellular microfilament actin, and the activity of alkaline phosphatase and expression level of the osteogenic gene Runx2 after osteogenic differentiation were detected. RESULTS AND CONCLUSION: (1) After 24 hours of culture, the cells in the mineralized collagen group, and 5 and 10 mmol/L Mg2++ mineralized collagen groups adhered well, which showed no significant difference from the blank control group, and the elongated spindle cells with many synapses linked to the adjacent cells were observed. The cells in the 20 mmol/L Mg2++ mineralized collagen group showed obvious pyknosis. (2) After 1, 3 and 5 days of culture, the cell viability in the 10 mmol/L Mg2++mineralized collagen group was significantly higher than that in the other four groups (P < 0.05). There was no significant difference among mineralized collagen, 5 mmoVL Mg2++ mineralized collagen and blank control groups (P > 0.05). The cell viability in the 20 mmol/L Mg2++ mineralized collagen group was significantly lower than that in the mineralized collagen group (P < 0.05). (3) After 3 days of culture, DAPI staining showed that 20 mmol/L Mg2++ mineralized collagen group had obvious nuclear disintegration, the other four groups had no obvious nuclear disintegration. (4) After 24 hours of culture, phalloidin staining showed that except the blank control and 20 mmol/L Mg2++mineralized collagen groups, the other three groups showed completely extended cell structure, and clear actin microfilaments, especially the 10 mmol/L Mg2++mineralized collagen group. (5) After 7 days of osteogenic differentiation, except for 20 mmol/L Mg2++ mineralized collagen group, the activity of alkaline phosphatase and the expression level of Runx2 gene in the other three groups were significantly higher than those in the blank control group (P < 0.05), and those in the 10mmol/L Mg2++ mineralized collagen group was significantly higher than those in the 5 mmol/L Mg2++mineralized collagen and mineralized collagen groups (P < 0.05). (6) These results suggest that the combination of magnesium ion with mineralized collagen should be applied with appropriate concentration range of magnesium ion （≤ 10mmol/L). [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan University of Science & Technology, Natural Science is the property of Editorial Office of Journal of Henan University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan University of Science & Technology, Natural Science is the property of Editorial Office of Journal of Henan University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)