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Objective To investigate the expression and signaling network of LncRNA FAM83 H-AS1 in endometrial carcinoma (EC) . Methods Cancer tissues and adjacent tissues from 82 patients with esophageal cancer (EC) were collected for detection of the expression levels of FAM83 H-AS1. The diagnostic value of FAM83 H-AS1 in EC was analyzed by ROC curve, and the correlation between FAM83 H-AS1 and clinical pathological data of EC patients was also analyzed. Long non-coding RNA (LncRNA) SNP sites were used to construct an LncRNA-miRNA interaction network. The GEPIA website was used to predict the co-expressed genes of FAM83 H-AS1, and KEGG enrichment analysis was conducted to determine the pathways enriched in co-expressed genes of FAM83 H-AS1. The correlations between FAM83 H-AS1, patient prognosis, and immunity was predicted. The expression of FAM83 H-AS1 in EC cells was intervened, and cell proliferation activity was detected using the Cell Counting Kit-8 (CCK-8) method, while cell invasion was assessed using Transwell. Results Compared with adjacent tissues, FAM83 H-AS1 expression was enhanced in cancer tissues (P＜0.05), and FAM83 H-AS1 could serve as an effective molecular marker for EC diagnosis (AUC=0.841, P＜0.01) . The expression of FAM83 H-AS1 was related to the clinical staging and depth of muscle invasion in EC patients (both P＜0.05) . Multiple miRNAs and mRNAs were found to be associated with FAM83 H-AS1, and the co-expressed genes of FAM83 H-AS1 were mainly enriched in pathways such as the cell cycle, endocytosis, and RNA degradation. UALCAN website predicted that patients with high expression of FAM83 H-AS1 had a poorer prognosis, and Timer website predicted that the expression of FAM83 H-AS1 was associated with CD8+ T cells and macrophages. Compared with the Blank group, overexpression of FAM83 H-AS1 promoted the proliferation and invasion of EC cells, while knockdown of FAM83 H-AS1 inhibited the proliferation and invasion of EC cells (all P＜0.05) . Conclusion FAM83 H-AS1 may be a carcinogenic driver in EC and could serve as a potential therapeutic target for treating EC. [ABSTRACT FROM AUTHOR]

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